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- PDB-7c89: Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) C50S/F80C/C207S/C2... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7c89 | |||||||||
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Title | Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) C50S/F80C/C207S/C213S mutant modified with 2-bromoacetophenone(Ph@ApPrx*) | |||||||||
![]() | Peroxiredoxin | |||||||||
![]() | OXIDOREDUCTASE / Peroxiredoxin | |||||||||
Function / homology | ![]() thioredoxin-dependent peroxiredoxin / peroxiredoxin activity / thioredoxin peroxidase activity / antioxidant activity / cell redox homeostasis / hydrogen peroxide catabolic process / cellular response to hydrogen peroxide / identical protein binding / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Himiyama, T. / Nakamura, T. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Rebuilding Ring-Type Assembly of Peroxiredoxin by Chemical Modification. Authors: Himiyama, T. / Tsuchiya, Y. / Yonezawa, Y. / Nakamura, T. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 525.4 KB | Display | ![]() |
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PDB format | ![]() | 419.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7c87C ![]() 7c8aC ![]() 7cqjC ![]() 6krkS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28651.797 Da / Num. of mol.: 10 / Mutation: C50S, F80C, C207S, C213S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-FLC / #3: Chemical | ChemComp-FL0 / #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.61 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: The reservoir solution contained 0.10 M sodium citrate (pH5.5) 0.20 M lithium sulfate 15% (v/v) reagent alcohol. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 12, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→50 Å / Num. obs: 168919 / % possible obs: 98.3 % / Redundancy: 3.6 % / CC1/2: 0.996 / Rmerge(I) obs: 0.08 / Net I/σ(I): 10.1 |
Reflection shell | Resolution: 2.1→2.14 Å / Rmerge(I) obs: 0.391 / Mean I/σ(I) obs: 3 / Num. unique obs: 8244 / CC1/2: 0.889 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6KRK Resolution: 2.1→49.168 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.939 / SU B: 4.406 / SU ML: 0.115 / Cross valid method: THROUGHOUT / ESU R: 0.179 / ESU R Free: 0.163 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL PLUS MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.297 Å2
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Refinement step | Cycle: LAST / Resolution: 2.1→49.168 Å
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Refine LS restraints |
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LS refinement shell |
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