+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-7341 | |||||||||
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タイトル | Conformation of methylated GGQ in the peptidyl transferase center during translation termination | |||||||||
マップデータ | non-stop termination complex | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 translation release factor activity, codon specific / negative regulation of cytoplasmic translational initiation / stringent response / ribosomal large subunit binding / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation ...translation release factor activity, codon specific / negative regulation of cytoplasmic translational initiation / stringent response / ribosomal large subunit binding / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / misfolded RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis / negative regulation of cytoplasmic translation / four-way junction DNA binding / translational termination / translation repressor activity / negative regulation of translational initiation / regulation of mRNA stability / mRNA regulatory element binding translation repressor activity / rescue of stalled ribosome / ribosome assembly / positive regulation of RNA splicing / assembly of large subunit precursor of preribosome / transcription elongation factor complex / regulation of DNA-templated transcription elongation / cytosolic ribosome assembly / DNA endonuclease activity / response to reactive oxygen species / transcription antitermination / translational initiation / regulation of cell growth / DNA-templated transcription termination / maintenance of translational fidelity / response to radiation / mRNA 5'-UTR binding / ribosomal small subunit biogenesis / large ribosomal subunit / small ribosomal subunit rRNA binding / ribosome biogenesis / ribosome binding / regulation of translation / ribosomal small subunit assembly / ribosomal large subunit assembly / small ribosomal subunit / transferase activity / large ribosomal subunit rRNA binding / 5S rRNA binding / cytosolic small ribosomal subunit / cytoplasmic translation / cytosolic large ribosomal subunit / tRNA binding / molecular adaptor activity / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / viral translational frameshifting / translation / response to antibiotic / negative regulation of DNA-templated transcription / mRNA binding / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | Escherichia coli (大腸菌) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.24 Å | |||||||||
データ登録者 | Zeng F / Jin H | |||||||||
資金援助 | 米国, 1件
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引用 | ジャーナル: Sci Rep / 年: 2018 タイトル: Conformation of methylated GGQ in the Peptidyl Transferase Center during Translation Termination. 著者: Fuxing Zeng / Hong Jin / 要旨: The universally conserved Gly-Gly-Gln (GGQ) tripeptide in release factors or release factor-like surveillance proteins is required to catalyze the release of nascent peptide in the ribosome. The ...The universally conserved Gly-Gly-Gln (GGQ) tripeptide in release factors or release factor-like surveillance proteins is required to catalyze the release of nascent peptide in the ribosome. The glutamine of the GGQ is methylated post-translationally at the N position in vivo; this covalent modification is essential for optimal cell growth and efficient translation termination. However, the precise conformation of the methylated-GGQ tripeptide in the ribosome remains unknown. Using cryoEM and X-ray crystallography, we report the conformation of methylated-GGQ in the peptidyl transferase center of the ribosome during canonical translational termination and co-translation quality control. It has been suggested that the GGQ motif arose independently through convergent evolution among otherwise unrelated proteins that catalyze peptide release. The requirement for this tripeptide in the highly conserved peptidyl transferase center suggests that the conformation reported here is likely shared during termination of protein synthesis in all domains of life. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_7341.map.gz | 202.5 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-7341-v30.xml emd-7341.xml | 121.7 KB 121.7 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_7341_fsc.xml | 13.6 KB | 表示 | FSCデータファイル |
画像 | emd_7341.png | 95.4 KB | ||
その他 | emd_7341_half_map_1.map.gz emd_7341_half_map_2.map.gz | 202.3 MB 202.3 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-7341 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-7341 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_7341_validation.pdf.gz | 941.3 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_7341_full_validation.pdf.gz | 940.8 KB | 表示 | |
XML形式データ | emd_7341_validation.xml.gz | 21.2 KB | 表示 | |
CIF形式データ | emd_7341_validation.cif.gz | 28.1 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-7341 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-7341 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_7341.map.gz / 形式: CCP4 / 大きさ: 216 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | non-stop termination complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-ハーフマップ: non-stop termination complex, half map #1
ファイル | emd_7341_half_map_1.map | ||||||||||||
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注釈 | non-stop termination complex, half map #1 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: non-stop termination complex, half map #2
ファイル | emd_7341_half_map_2.map | ||||||||||||
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注釈 | non-stop termination complex, half map #2 | ||||||||||||
投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : nonstop ribosomal complex with ArfA/RF2
+超分子 #1: nonstop ribosomal complex with ArfA/RF2
+超分子 #2: 50S subunit
+超分子 #3: 30S subunit
+超分子 #11: 30S ribosomal proteins
+超分子 #12: 50S ribosomal proteins
+超分子 #4: Peptide chain release factor 2
+超分子 #5: Alternative ribosome-rescue factor A
+超分子 #6: E-site or P-site tRNA fMet
+超分子 #7: nonstop mRNA
+超分子 #8: 23S rRNA
+超分子 #9: 5S rRNA
+超分子 #10: 16S rRNA
+超分子 #13: 50S ribosomal protein L2
+超分子 #14: 50S ribosomal protein L3
+超分子 #15: 50S ribosomal protein L4
+超分子 #16: 50S ribosomal protein L5
+超分子 #17: 50S ribosomal protein L6
+超分子 #18: 50S ribosomal protein L9
+超分子 #19: 50S ribosomal protein L10
+超分子 #20: 50S ribosomal protein L11
+超分子 #21: 50S ribosomal protein L13
+超分子 #22: 50S ribosomal protein L14
+超分子 #23: 50S ribosomal protein L15
+超分子 #24: 50S ribosomal protein L16
+超分子 #25: 50S ribosomal protein L17
+超分子 #26: 50S ribosomal protein L18
+超分子 #27: 50S ribosomal protein L19
+超分子 #28: 50S ribosomal protein L20
+超分子 #29: 50S ribosomal protein L21
+超分子 #30: 50S ribosomal protein L22
+超分子 #31: 50S ribosomal protein L23
+超分子 #32: 50S ribosomal protein L24
+超分子 #33: 50S ribosomal protein L25
+超分子 #34: 50S ribosomal protein L27
+超分子 #35: 50S ribosomal protein L28
+超分子 #36: 50S ribosomal protein L29
+超分子 #37: 50S ribosomal protein L30
+超分子 #38: 50S ribosomal protein L31
+超分子 #39: 50S ribosomal protein L32
+超分子 #40: 50S ribosomal protein L33
+超分子 #41: 50S ribosomal protein L34
+超分子 #42: 50S ribosomal protein L35
+超分子 #43: 50S ribosomal protein L36
+超分子 #44: 30S ribosomal protein S2
+超分子 #45: 30S ribosomal protein S3
+超分子 #46: 30S ribosomal protein S4
+超分子 #47: 30S ribosomal protein S5
+超分子 #48: 30S ribosomal protein S6
+超分子 #49: 30S ribosomal protein S7
+超分子 #50: 30S ribosomal protein S8
+超分子 #51: 30S ribosomal protein S9
+超分子 #52: 30S ribosomal protein S10
+超分子 #53: 30S ribosomal protein S11
+超分子 #54: 30S ribosomal protein S12
+超分子 #55: 30S ribosomal protein S13
+超分子 #56: 30S ribosomal protein S14
+超分子 #57: 30S ribosomal protein S15
+超分子 #58: 30S ribosomal protein S16
+超分子 #59: 30S ribosomal protein S17
+超分子 #60: 30S ribosomal protein S18
+超分子 #61: 30S ribosomal protein S19
+超分子 #62: 30S ribosomal protein S20
+超分子 #63: 30S ribosomal protein S21
+分子 #1: 23S rRNA
+分子 #2: 5S rRNA
+分子 #34: 16S rRNA
+分子 #57: E-site or P-site tRNA fMet
+分子 #58: mRNA
+分子 #3: 50S ribosomal protein L2
+分子 #4: 50S ribosomal protein L3
+分子 #5: 50S ribosomal protein L4
+分子 #6: 50S ribosomal protein L5
+分子 #7: 50S ribosomal protein L6
+分子 #8: 50S ribosomal protein L9
+分子 #9: 50S ribosomal protein L10
+分子 #10: 50S ribosomal protein L11
+分子 #11: 50S ribosomal protein L13
+分子 #12: 50S ribosomal protein L14
+分子 #13: 50S ribosomal protein L15
+分子 #14: 50S ribosomal protein L16
+分子 #15: 50S ribosomal protein L17
+分子 #16: 50S ribosomal protein L18
+分子 #17: 50S ribosomal protein L19
+分子 #18: 50S ribosomal protein L20
+分子 #19: 50S ribosomal protein L21
+分子 #20: 50S ribosomal protein L22
+分子 #21: 50S ribosomal protein L23
+分子 #22: 50S ribosomal protein L24
+分子 #23: 50S ribosomal protein L25
+分子 #24: 50S ribosomal protein L27
+分子 #25: 50S ribosomal protein L28
+分子 #26: 50S ribosomal protein L29
+分子 #27: 50S ribosomal protein L30
+分子 #28: 50S ribosomal protein L31
+分子 #29: 50S ribosomal protein L32
+分子 #30: 50S ribosomal protein L33
+分子 #31: 50S ribosomal protein L34
+分子 #32: 50S ribosomal protein L35
+分子 #33: 50S ribosomal protein L36
+分子 #35: 30S ribosomal protein S2
+分子 #36: 30S ribosomal protein S3
+分子 #37: 30S ribosomal protein S4
+分子 #38: 30S ribosomal protein S5
+分子 #39: 30S ribosomal protein S6
+分子 #40: 30S ribosomal protein S7
+分子 #41: 30S ribosomal protein S8
+分子 #42: 30S ribosomal protein S9
+分子 #43: 30S ribosomal protein S10
+分子 #44: 30S ribosomal protein S11
+分子 #45: 30S ribosomal protein S12
+分子 #46: 30S ribosomal protein S13
+分子 #47: 30S ribosomal protein S14
+分子 #48: 30S ribosomal protein S15
+分子 #49: 30S ribosomal protein S16
+分子 #50: 30S ribosomal protein S17
+分子 #51: 30S ribosomal protein S18
+分子 #52: 30S ribosomal protein S19
+分子 #53: 30S ribosomal protein S20
+分子 #54: 30S ribosomal protein S21
+分子 #55: Peptide chain release factor 2
+分子 #56: Alternative ribosome-rescue factor A
+分子 #59: MAGNESIUM ION
+分子 #60: ZINC ION
+分子 #61: water
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.5 構成要素:
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グリッド | モデル: C-flat-2/0.5 4C / 前処理 - タイプ: GLOW DISCHARGE | |||||||||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV / 詳細: Grids were blotted for 3.5 seconds.. |
-電子顕微鏡法
顕微鏡 | JEOL 3200FS |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 検出モード: SUPER-RESOLUTION / 平均電子線量: 20.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 倍率(補正後): 83822 / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
試料ステージ | ホルダー冷却材: NITROGEN |