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Basic information
Entry | Database: PDB / ID: 6z9q | ||||||||||||
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Title | Transcription termination intermediate complex 2 | ||||||||||||
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![]() | TRANSCRIPTION / Termination / Helicase / RNA Polymerase | ||||||||||||
Function / homology | ![]() bacterial-type RNA polymerase core enzyme binding / ATP-dependent activity, acting on RNA / transcription elongation-coupled chromatin remodeling / RNA polymerase complex / submerged biofilm formation / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / bacterial-type flagellum-dependent cell motility ...bacterial-type RNA polymerase core enzyme binding / ATP-dependent activity, acting on RNA / transcription elongation-coupled chromatin remodeling / RNA polymerase complex / submerged biofilm formation / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / regulation of DNA-templated transcription initiation / bacterial-type flagellum assembly / bacterial-type flagellum-dependent cell motility / nitrate assimilation / translation elongation factor activity / DNA-directed RNA polymerase complex / helicase activity / transcription elongation factor complex / regulation of DNA-templated transcription elongation / transcription antitermination / DNA-templated transcription initiation / cell motility / DNA-templated transcription termination / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / ribosome biogenesis / protein complex oligomerization / response to heat / protein-containing complex assembly / intracellular iron ion homeostasis / protein dimerization activity / DNA-binding transcription factor activity / protein domain specific binding / response to antibiotic / nucleotide binding / DNA-templated transcription / magnesium ion binding / ATP hydrolysis activity / DNA binding / RNA binding / zinc ion binding / ATP binding / identical protein binding / membrane / cytosol / cytoplasm Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() synthetic construct (others) | ||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 5.7 Å | ||||||||||||
![]() | Said, N. / Hilal, T. / Loll, B. / Wahl, M.C. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase ρ. Authors: Nelly Said / Tarek Hilal / Nicholas D Sunday / Ajay Khatri / Jörg Bürger / Thorsten Mielke / Georgiy A Belogurov / Bernhard Loll / Ranjan Sen / Irina Artsimovitch / Markus C Wahl / ![]() ![]() ![]() ![]() Abstract: Factor-dependent transcription termination mechanisms are poorly understood. We determined a series of cryo-electron microscopy structures portraying the hexameric adenosine triphosphatase (ATPase) ...Factor-dependent transcription termination mechanisms are poorly understood. We determined a series of cryo-electron microscopy structures portraying the hexameric adenosine triphosphatase (ATPase) ρ on a pathway to terminating NusA/NusG-modified elongation complexes. An open ρ ring contacts NusA, NusG, and multiple regions of RNA polymerase, trapping and locally unwinding proximal upstream DNA. NusA wedges into the ρ ring, initially sequestering RNA. Upon deflection of distal upstream DNA over the RNA polymerase zinc-binding domain, NusA rotates underneath one capping ρ subunit, which subsequently captures RNA. After detachment of NusG and clamp opening, RNA polymerase loses its grip on the RNA:DNA hybrid and is inactivated. Our structural and functional analyses suggest that ρ, and other termination factors across life, may use analogous strategies to allosterically trap transcription complexes in a moribund state. | ||||||||||||
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-Validation report
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-Related structure data
Related structure data | ![]() 11088MC ![]() 6z9pC ![]() 6z9rC ![]() 6z9sC ![]() 6z9tC ![]() 7adbC ![]() 7adcC ![]() 7addC ![]() 7adeC M: map data used to model this data C: citing same article ( |
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Components
-Transcription termination/antitermination protein ... , 2 types, 2 molecules GA
#1: Protein | Mass: 20560.523 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: nusG, A6592_25585, A6V01_22710, A8C65_22140, A9819_22730, A9P13_25180, A9R57_05240, A9X72_23950, AC067_25565, AC789_1c43770, ACN002_4069, ACN68_05355, ACN81_23140, ACU57_23215, ACU90_26230, ...Gene: nusG, A6592_25585, A6V01_22710, A8C65_22140, A9819_22730, A9P13_25180, A9R57_05240, A9X72_23950, AC067_25565, AC789_1c43770, ACN002_4069, ACN68_05355, ACN81_23140, ACU57_23215, ACU90_26230, AM270_23415, AM446_26285, AM464_13235, AML07_02765, AML35_27155, APT94_08685, APZ14_17375, ARC77_16990, AU473_00875, AUQ13_12310, AUS26_26490, AW059_24245, AW106_22725, AWB10_24980, AWG90_024840, AWP75_07120, B7C53_21495, B9M99_23250, B9T59_11605, BANRA_02652, BANRA_02965, BANRA_04868, BANRA_05054, BB545_03125, BE963_11245, BEN53_24665, BER14_24185, BHF03_13055, BHF46_22020, BHS81_23795, BHS87_22260, BIQ87_22825, BIU72_18845, BIZ41_20425, BJJ90_25150, BK248_06370, BK292_28055, BK296_22625, BK334_23885, BK373_27240, BK375_25285, BK383_27750, BMT91_03315, BN17_39461, BOH76_00570, BON63_20075, BON65_15050, BON66_14345, BON69_07640, BON71_08905, BON72_17240, BON75_02205, BON76_16185, BON83_01585, BON86_02250, BON87_25635, BON92_00555, BON94_22220, BON95_12895, BON96_11035, BTQ04_24460, BTQ06_27300, BUE81_05395, BvCms12BK_01423, BvCms2454_04763, BvCms28BK_04512, BvCms35BK_02101, BvCmsC61A_02144, BvCmsHHP001_05230, BvCmsHHP019_04457, BvCmsHHP056_02613, BvCmsKKP036_03586, BvCmsKKP061_02988, BvCmsKSNP073_02860, BvCmsKSNP081_04473, BvCmsKSNP120_03442, BvCmsKSP011_02762, BvCmsKSP024_02914, BvCmsKSP026_03368, BvCmsKSP045_04193, BvCmsKSP058_05150, BvCmsKSP067_02572, BvCmsKSP076_03394, BvCmsNSNP036_01151, BvCmsNSP006_02609, BvCmsNSP007_05025, BvCmsNSP047_00265, BvCmsNSP072_02887, BvCmsOUP014_03194, BvCmsSINP011_03926, BvCmsSINP022_04149, BvCmsSIP019_00806, BvCmsSIP044_03280, BVL39_05395, BW690_26720, BWI89_17415, BWP17_22740, BXT93_25190, BZL31_04420, BZL69_05760, C2M16_25145, C2U48_12750, C4M78_26060, C5715_03885, C5N07_24575, C5P01_26085, C5P44_24205, C6669_07630, C6B13_24055, C7235_23740, C7B02_25750, C7B06_24590, C7B07_24515, C7B08_25795, C7B18_24885, C9098_22125, C9114_22875, C9141_23580, C9160_23410, C9162_26315, C9201_22170, C9306_18330, C9E25_22050, C9E67_28690, C9Z03_20290, C9Z23_22460, C9Z28_21960, C9Z29_22205, C9Z37_20890, C9Z39_11545, C9Z68_22005, C9Z70_22895, C9Z78_23210, C9Z89_21250, CA593_06055, CDC27_21525, CDL37_18180, CF006_23900, CG692_19490, CI641_013295, CI693_09880, CI694_22430, CIG45_23855, CJU63_23510, CMR93_21685, CO706_19475, COD30_25685, COD46_23680, CQB02_21475, CQP61_26065, CR538_24245, CR539_01640, CRD98_23200, CRE06_24015, CRM83_18660, CRX46_23015, CSB64_25615, CV83915_02071, CVH05_03840, CWM24_27465, CWS33_24585, D0X26_26090, D2184_25125, D2185_24315, D2188_26625, D3821_09130, D3822_07045, D3O91_23455, D3P01_24300, D3Y67_18010, D4M06_24755, D4V08_22465, D6T60_24420, D6X76_25425, D7K63_24265, D7K66_23575, D9D20_23600, D9D31_17400, D9D43_22285, D9E34_21740, D9E35_23840, D9F17_24210, D9G11_22910, D9G42_23575, D9G48_16785, D9H36_23700, D9H53_22650, D9H68_20300, D9H70_22035, D9H94_18920, D9I18_21340, D9I87_21115, D9I88_18975, D9I97_19355, D9J03_21550, D9J11_22250, D9J44_24490, D9J60_24680, D9J78_24465, D9K48_24855, D9K54_12200, D9L89_22830, D9X97_23540, DAH18_24890, DAH26_25975, DAH30_23810, DAH32_23215, DAH34_21590, DAH37_22535, DAH43_24845, DB359_25595, DBQ99_24575, DD762_22315, DEN86_24465, DEN89_26545, DEN97_23745, DEO04_24340, DEO19_24590, DIV22_03710, DJ492_21155, DJ503_21480, DL455_22945, DL545_23715, DL800_27635, DM102_24845, DM129_21405, DMI04_23160, DN627_23120, DN700_22935, DNB37_22335, DND16_22055, DNQ45_05150, DNX30_24180, DOY56_23490, DP258_23400, DP277_21350, DQE83_24500, DQF57_23755, DQO13_23745, DS732_01515, DTL43_13735, DTL90_24565, DTM10_25890, DTM45_24265, DU309_24235, DU321_23455, DWB25_23615, DXT69_22770, DXT71_22275, DXT73_22975, DXX80_014070, E0I42_21930, E0K84_22915, E0L12_22075, E2112_20390, E2115_21525, E2116_21360, E2119_22370, E2127_20870, E2128_03155, E2129_21325, E2134_22760, E2135_19300, E2855_04995, E2863_04087, E4K55_23315, E5P22_12620, E5P28_08855, E5P37_21480, E5S42_21570, E5S46_22920, E5S47_23055, E5S58_21655, E5S61_22405, EA223_18780, EAI42_07555, EAI46_11730, EAI52_22765, EAX79_24725, EB509_19910, EB510_23015, EB515_23215, EC1094V2_4288, EC3234A_76c00020, EC3426_00293, EC382_20035, EC95NR1_03447, ECONIH1_23440, ECs4905, ECTO6_04524, ED225_22815, ED600_17570, ED607_18465, ED611_23905, ED648_18875, ED903_15555, ED944_23285, EEA45_21070, EEP23_09520, EF173_24425, EHH55_00685, EHJ36_22675, EI021_22100, EI028_22550, EI032_21280, EIA08_23380, EIA21_22555, EIZ93_16305, EJC75_19605, EKI52_13975, EL75_4207, EL79_4413, EL80_4320, ELT20_20690, ELT49_22095, ELT58_20800, ELU85_23620, ELV08_18720, ELV15_18010, ELV28_22220, ELY05_18135, EO241_25805, EPT01_21710, EPU41_22430, EQ820_22900, EQ823_21595, EQ825_24495, EQ830_22075, ERL57_22710, ERS085365_04268, ERS085366_03833, ERS085374_04594, ERS085379_04702, ERS085386_04623, ERS085404_04521, ERS085416_04255, ERS139211_00029, ERS150873_04413, ERS150876_04842, EST51_23890, EVY14_16870, EXM29_02430, ExPECSC019_03691, ExPECSC038_04333, EXX06_24555, EXX13_24135, EXX23_22335, EXX24_23200, EXX53_20975, EXX55_24470, EXX71_22285, EXX78_23980, EXX87_25010, EYD11_21620, EYX82_16660, EYY27_22340, EYY34_19835, EYY78_22630, F1E13_23780, F1E19_21855, F7F18_24025, F7F23_23365, F7F29_25900, F7G01_23380, F7G03_24045, FAF34_028990, FKO60_24845, FNJ69_19080, FNJ83_24770, FORC82_4527, FQ915_26180, FQR64_21830, FRV13_16245, FV293_23400, FV295_22235, FV438_22405, FWK02_12320, FY127_17780, FZ043_01110, GJ11_25170, HmCms184_03867, HmCmsJML074_03188, HmCmsJML079_04908, HmCmsJML204_03263, HMPREF3040_01771, HW43_01555, MJ49_09455, MS6198_46010, NCTC10082_02087, NCTC10090_02786, NCTC10429_00094, NCTC10764_02978, NCTC10766_02647, NCTC10865_05901, NCTC10974_05357, NCTC11022_04295, NCTC11112_02275, NCTC11126_05446, NCTC11181_01971, NCTC11341_02768, NCTC12950_05220, NCTC13127_06170, NCTC13148_04401, NCTC13846_04507, NCTC7922_04992, NCTC7927_05255, NCTC8009_08439, NCTC8179_05103, NCTC8450_00357, NCTC8500_05331, NCTC8622_01622, NCTC8960_02346, NCTC9007_05346, NCTC9036_04655, NCTC9044_02298, NCTC9045_05548, NCTC9050_02824, NCTC9055_01673, NCTC9058_02141, NCTC9062_03438, NCTC9077_05942, NCTC9111_04961, NCTC9117_05910, NCTC9119_05061, NCTC9701_05002, NCTC9702_05645, NCTC9703_04220, NCTC9706_02009, NCTC9777_01124, NCTC9969_04938, PGD_00616, PU06_15460, RG28_25315, RK56_017435, RX35_03749, SAMEA3472043_04873, SAMEA3472044_04880, SAMEA3472047_03493, SAMEA3472055_04940, SAMEA3472056_05316, SAMEA3472070_05100, SAMEA3472080_04808, SAMEA3472090_05013, SAMEA3472108_04978, SAMEA3472110_05075, SAMEA3472112_05160, SAMEA3472114_04960, SAMEA3472147_04113, SAMEA3484427_04977, SAMEA3484429_04980, SAMEA3484434_04732, SAMEA3485101_05504, SAMEA3485113_04488, SAMEA3752372_05072, SAMEA3752553_04898, SAMEA3752557_04837, SAMEA3752559_04862, SAMEA3752620_04706, SAMEA3753064_05336, SAMEA3753097_04838, SAMEA3753164_04793, SAMEA3753290_00011, SAMEA3753300_04891, SK85_04313, UC41_25620, UN86_14210, UN91_25475, WQ89_24645, WR15_01460, YDC107_2696 Production host: ![]() ![]() References: UniProt: C3SID2, UniProt: P0AFG0*PLUS |
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#3: Protein | Mass: 55060.812 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: nusA, A6581_13525, A8C65_02280, A9819_18195, A9R57_12710, A9X72_02950, AC789_1c35390, ACN002_3262, ACN68_12470, ACN81_18560, ACU57_14555, ACU90_11675, AJ318_05130, AM270_15620, AM464_08470, ...Gene: nusA, A6581_13525, A8C65_02280, A9819_18195, A9R57_12710, A9X72_02950, AC789_1c35390, ACN002_3262, ACN68_12470, ACN81_18560, ACU57_14555, ACU90_11675, AJ318_05130, AM270_15620, AM464_08470, AM465_07745, AMK83_06450, AML35_13995, ARC77_12295, AU473_05845, AUQ13_02965, AW059_15285, AWB10_02965, AWE53_007755, AWF59_011800, AWG78_015835, AZZ83_001443, B6V57_17910, B7C53_06460, B9N33_16970, BANRA_01789, BANRA_03826, BB545_09890, BFD68_02465, BHF03_08610, BHF46_07205, BHS87_17855, BIQ87_17925, BIU72_05295, BIZ41_14490, BJJ90_02880, BK373_10285, BK375_17175, BK383_15855, BMT91_09130, BN17_31141, BOH76_04800, BON63_14350, BON65_19800, BON66_12095, BON69_26670, BON71_25285, BON72_14050, BON75_23260, BON76_05640, BON94_12450, BON95_22130, BON96_06470, BTQ04_16590, BTQ06_11760, BUE81_19575, BvCms12BK_01027, BvCms2454_04844, BvCms28BK_03352, BvCms35BK_01521, BvCmsC61A_01481, BvCmsHHP001_01224, BvCmsHHP019_03131, BvCmsHHP056_02506, BvCmsKKP036_00266, BvCmsKKP061_00866, BvCmsKSNP073_02140, BvCmsKSNP081_00264, BvCmsKSNP120_03552, BvCmsKSP011_02072, BvCmsKSP024_00305, BvCmsKSP026_00480, BvCmsKSP045_01628, BvCmsKSP058_01862, BvCmsKSP067_01442, BvCmsKSP076_01608, BvCmsNSNP036_02129, BvCmsNSP006_04618, BvCmsNSP007_02229, BvCmsNSP047_04140, BvCmsOUP014_03610, BvCmsSINP011_04480, BvCmsSINP022_03541, BvCmsSIP019_00385, BvCmsSIP044_00115, BW690_05715, BWI89_22180, BWP17_10040, BXT93_11865, BZL31_05810, C2U48_17660, C4K41_03670, C5N07_18180, C5P44_00540, C7B06_16690, C7B07_10710, C9098_07900, C9114_15345, C9162_04220, C9E25_11380, C9E67_03485, C9Z23_02315, C9Z28_00495, C9Z29_07080, C9Z37_14770, C9Z39_14385, C9Z69_11065, C9Z70_06875, C9Z78_03425, CCZ14_12145, CCZ17_15255, CF006_16780, CG692_07185, CI693_03560, CI694_20950, CIG45_16160, CMR93_08950, CO706_14520, COD46_10645, CR538_03185, CR539_21135, CRD98_04825, CRM83_23610, CRT43_19205, CT146_02945, CUB99_16245, CVH05_10890, D2184_10395, D2185_00555, D3821_08435, D3C88_08620, D3O91_07735, D3Y67_18400, D4M06_11115, D6X76_04225, D7K63_12110, D7Y10_03480, D9D20_06165, D9D31_09845, D9D33_00520, D9E34_10725, D9E35_01735, D9E49_09485, D9F17_07090, D9G42_16790, D9H53_01085, D9H68_07450, D9H70_01530, D9I18_07065, D9I87_04950, D9I88_16540, D9I97_11390, D9J11_01020, D9J44_07740, D9J46_20540, D9J78_05020, D9K54_17930, D9L99_02550, DAH26_17205, DAH30_12985, DAH34_01870, DAH37_04365, DAH43_16670, DD762_19130, DEN89_09295, DEN97_16025, DEO04_19495, DEO19_06320, DJ487_12875, DJ503_10865, DL455_12590, DL545_03770, DMI04_04160, DMZ50_12310, DN627_01240, DN700_04485, DNB37_13595, DNR41_11075, DOY56_12135, DP258_01950, DQF57_03505, DQO13_01185, DS732_23585, DS966_19180, DTL43_04185, DTL90_07460, DTM10_07095, DU333_16810, DW236_11975, DXT69_05630, DXT71_00550, DXT73_13270, DXX80_009480, E0I42_14230, E0L04_19775, E2119_05760, E2127_02465, E2128_02605, E2129_05650, E2134_15705, E2135_07970, E5P22_12885, E5P28_13020, E5P37_13245, E5S42_11905, E5S47_02870, E5S61_05815, EA223_04935, EA250_06525, EA834_14925, EAI42_15045, EAI52_10000, EAX79_13120, EB509_07090, EB510_12295, EC1094V2_475, EC3234A_53c00480, EC382_14545, ECs4050, ECTO124_00620, ECTO6_00587, ED225_04160, ED607_10315, ED611_04170, ED648_02805, EF173_16895, EGT48_12340, EI021_03955, EI028_15160, EI032_08695, EIA21_02770, EIZ93_15765, EL75_0516, EL79_0538, EL80_0529, ELT20_05155, ELT23_06875, ELT33_08795, ELT34_10430, ELT48_07010, ELU82_09405, ELU96_15075, ELV05_02165, ELV13_06455, ELV24_06465, ELX79_02850, ELX83_01985, ELY05_03160, ELY23_12925, ELY24_06185, ELY50_10980, EO241_09470, EPS76_01475, EPS94_08345, EPT01_00525, EQ825_16425, ERS085365_00562, ERS085374_01906, ERS085379_00395, ERS085383_00719, ERS085386_00510, ERS085404_02209, ERS085406_02909, ERS085416_00332, ERS139211_00573, ERS150873_04324, EWK56_02885, EXM29_19885, ExPECSC065_00153, EXX06_09580, EXX13_07225, EXX55_08945, EXX71_13290, EXX78_16840, EXX87_04480, EYD11_02755, EYX82_02345, EYX99_26665, EYY34_08675, F1E19_22155, F7F18_01095, F7G01_14090, F7G03_16260, FKO60_06130, FNJ67_03685, FORC82_0590, FQ915_16570, FQR64_03145, FQU83_04620, FRV13_20655, FV293_08485, FV438_11000, FY127_14855, FZ043_21925, HmCms184_00231, HmCmsJML079_04422, HmCmsJML146_01153, HmCmsJML204_02228, HW43_20805, MJ49_14455, MS8345_03518, NCTC10089_00629, NCTC10090_03662, NCTC10767_01660, NCTC10865_00813, NCTC10974_00712, NCTC11022_03334, NCTC11126_02933, NCTC11341_01740, NCTC13127_00931, NCTC8179_06097, NCTC8621_00649, NCTC8959_03134, NCTC9045_00717, NCTC9055_02514, NCTC9058_01244, NCTC9062_02552, NCTC9075_00900, NCTC9081_01077, NCTC9111_00998, NCTC9119_00655, NCTC9434_00566, NCTC9701_00709, NCTC9703_05100, NCTC9706_02868, NCTC9969_00791, PGD_03947, PU06_19960, RG28_20965, RK56_014720, RX35_04256, SAMEA3472044_03332, SAMEA3472047_01872, SAMEA3472055_00400, SAMEA3472056_03072, SAMEA3472080_00945, SAMEA3472090_00548, SAMEA3472108_00102, SAMEA3472114_00483, SAMEA3472147_01878, SAMEA3485101_01710, SAMEA3752553_01187, SAMEA3752557_01875, SAMEA3752559_03273, SAMEA3752620_02341, SAMEA3753064_02936, SAMEA3753097_03302, SAMEA3753164_02300, SAMEA3753290_00148, SAMEA3753300_02131, SK85_03482, SY51_18275, U12A_03405, U14A_03402, UC41_24435, UN86_15490, UN91_15505, WQ89_06750, WR15_24550 Production host: ![]() ![]() References: UniProt: C3SSN7, UniProt: P0AFF6*PLUS |
-DNA-directed RNA polymerase subunit ... , 4 types, 5 molecules UVWXY
#4: Protein | Mass: 36558.680 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() References: UniProt: P0A7Z4, DNA-directed RNA polymerase #5: Protein | | Mass: 10249.547 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() References: UniProt: P0A800, DNA-directed RNA polymerase #6: Protein | | Mass: 150820.875 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() References: UniProt: P0A8V4, UniProt: P0A8V2*PLUS, DNA-directed RNA polymerase #7: Protein | | Mass: 156537.031 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: rpoC, A6581_23995, A6592_25550, A6V01_22675, A8C65_22170, A9P13_25145, A9R57_05210, A9X72_23915, AC789_1c43830, ACN002_4075, ACN68_05320, ACN81_23175, ACU57_23185, ACU90_26200, AM270_23445, ...Gene: rpoC, A6581_23995, A6592_25550, A6V01_22675, A8C65_22170, A9P13_25145, A9R57_05210, A9X72_23915, AC789_1c43830, ACN002_4075, ACN68_05320, ACN81_23175, ACU57_23185, ACU90_26200, AM270_23445, AM446_26250, AM464_13270, AMK83_15405, AML07_02735, AML35_27125, APT94_08715, APZ14_17345, ARC77_17020, AU473_00845, AUQ13_12340, AUS26_26460, AW059_24210, AWB10_24945, AWE53_003330, AWG90_024870, AZZ83_004736, B7C53_21460, B9M99_23215, BANRA_02658, BANRA_02959, BANRA_04874, BANRA_05060, BB545_03160, BE963_11280, BHF03_13085, BHF46_21990, BHS81_23830, BHS87_22295, BIQ87_22860, BIU72_18810, BIZ41_20460, BJJ90_25120, BK292_28025, BK296_22655, BK373_27210, BK375_25315, BK383_27720, BMT49_21005, BMT91_03285, BN17_39521, BOH76_00535, BON63_20110, BON65_15085, BON66_14380, BON69_07675, BON71_08940, BON72_17275, BON75_02240, BON76_16220, BON83_01620, BON87_25670, BON91_27070, BON92_00590, BON94_22185, BON95_12860, BON96_11000, BTQ04_24495, BTQ06_27265, BUE81_05360, BvCms12BK_01429, BvCms2454_04769, BvCms28BK_04518, BvCms35BK_02095, BvCmsC61A_02150, BvCmsHHP001_05236, BvCmsHHP019_04451, BvCmsHHP056_02607, BvCmsKKP061_02982, BvCmsKSNP073_02854, BvCmsKSNP081_04479, BvCmsKSNP120_03436, BvCmsKSP011_02756, BvCmsKSP024_02908, BvCmsKSP026_03362, BvCmsKSP045_04187, BvCmsKSP058_05144, BvCmsKSP067_02566, BvCmsNSNP036_01157, BvCmsNSP006_02615, BvCmsNSP007_05019, BvCmsNSP047_00271, BvCmsNSP072_02893, BvCmsOUP014_03200, BvCmsSINP011_03932, BvCmsSINP022_04155, BvCmsSIP019_00812, BvCmsSIP044_03274, BVL39_05360, BW690_26755, BWI89_17380, BWP17_22705, BXT93_12730, BZL31_04385, BZL69_05725, C2U48_12715, C5715_03850, C5N07_24540, C5P01_26050, C5P44_24170, C7235_23705, C7B02_25785, C7B06_24625, C7B07_24550, C7B08_25760, C9098_22155, C9114_22905, C9141_23610, C9160_23380, C9162_26345, C9201_22200, C9306_18300, C9E25_22085, C9E67_28655, C9Z03_20320, C9Z23_22490, C9Z28_21990, C9Z29_22175, C9Z37_20920, C9Z39_11515, C9Z43_22460, C9Z69_21605, C9Z70_22865, CA593_06020, CDC27_21560, CF006_23865, CI641_013325, CI693_09915, CI694_22465, CIG45_23820, CJU63_23545, CMR93_21720, CO706_19510, COD30_25650, COD46_23645, CR538_24210, CR539_01675, CRD98_23235, CRE06_23980, CRM83_18625, CRX46_23050, CV83915_02065, CVH05_03875, CWS33_24550, D0X26_26125, D2184_25090, D2185_24280, D2188_26590, D3821_09095, D3822_07080, D3O91_23490, D3P01_24335, D3Y67_18045, D4M06_24790, D6T60_24455, D6X76_25460, D7K63_24300, D7K66_23610, D9D20_23630, D9D31_17430, D9D43_22315, D9E34_21770, D9E35_23870, D9F17_24240, D9G48_16815, D9H53_22620, D9H68_20330, D9H70_22065, D9H94_18950, D9I18_21370, D9I87_21145, D9I88_19005, D9I97_19385, D9J03_21580, D9J11_22220, D9J44_24520, D9J60_24650, D9J78_24495, D9K48_24885, D9L89_22865, D9X97_23575, DAH18_24925, DAH26_25940, DAH30_23845, DAH34_21555, DAH37_22570, DAH43_24880, DBQ99_24540, DD762_22280, DEN89_26510, DEN97_23710, DEO04_24305, DEO19_24555, DIV22_03745, DJ487_24740, DJ503_21515, DL455_22980, DL545_23680, DLW88_24480, DM102_24810, DMI04_23195, DN627_23155, DN700_22970, DNB37_22370, DND16_22020, DOY56_23525, DP258_23435, DP277_21385, DQE83_24465, DQF57_23790, DQO13_23780, DTL43_13700, DTL90_24530, DTM10_25855, DU309_24205, DU321_23425, DW236_24545, DXT69_22805, DXT71_22310, DXT73_22940, DXX80_014100, E0I42_21900, E0K84_22885, E0L12_22045, E2112_20360, E2119_22340, E2127_20900, E2128_03185, E2129_21295, E2134_22790, E2135_19330, E4K55_23345, E5P22_12650, E5P28_08825, E5P37_21450, E5S42_21540, E5S47_23025, E5S58_21685, E5S61_22375, EA223_18745, EA410_23470, EAI42_07590, EAI46_11695, EAI52_22800, EAX79_24760, EB509_19940, EB510_23045, EB515_23245, EC1094V2_4282, EC3234A_76c00080, EC3426_00300, EC382_20005, ECs4911, ECTO6_04517, ED225_22845, ED607_18495, ED611_23935, ED648_18905, ED903_15585, ED944_23315, EEA45_21100, EEP23_09490, EF173_24455, EHH55_00715, EHJ36_22705, EI021_22070, EI028_22520, EI032_21250, EIA21_22525, EIZ93_16335, EJC75_19635, EL75_4201, EL79_4407, EL80_4314, ELT20_20720, ELT23_22660, ELU85_23590, ELV08_18690, ELV28_22250, ELY05_18165, EO241_25775, EPT01_21680, EQ820_22870, EQ823_21565, ERS085365_04275, ERS085366_03840, ERS085374_04587, ERS085379_04695, ERS085383_04736, ERS085386_04630, ERS085404_04514, ERS085416_04248, ERS139211_00022, ERS150873_04406, ERS150876_04849, EST51_23860, EVY14_16840, EXM29_02400, EXX06_24525, EXX23_22305, EXX24_23170, EXX53_21005, EXX55_24440, EXX71_22255, EXX78_24010, EXX87_24980, EYD11_21590, EYX82_16690, EYY34_19805, EYY78_22660, F1E13_23750, F1E19_21825, F7F11_23815, F7F18_24055, F7F29_25930, F7G01_23350, F7G03_24075, FAF34_028960, FNJ69_19110, FNJ83_24800, FORC82_4521, FQ915_26150, FQR64_21800, FRV13_16215, FV293_23430, FV438_22435, FWK02_01680, FY127_17750, FZ043_01140, GJ11_25200, HmCms184_03861, HmCmsJML074_03182, HmCmsJML079_04902, HmCmsJML204_03257, HMPREF3040_01778, HW43_01585, MJ49_09425, NCTC10082_02080, NCTC10090_02779, NCTC10429_00081, NCTC10865_05894, NCTC10974_05350, NCTC11181_01964, NCTC12950_05213, NCTC13127_06163, NCTC13148_04414, NCTC7927_05248, NCTC8500_05324, NCTC8960_02339, NCTC9036_04648, NCTC9044_02305, NCTC9045_05541, NCTC9050_02817, NCTC9055_01666, NCTC9058_02148, NCTC9062_03445, NCTC9077_05932, NCTC9111_04954, NCTC9119_05054, NCTC9701_04995, NCTC9706_02002, NCTC9777_01117, NCTC9969_04931, PGD_00622, PU06_15490, RG28_25345, RK56_017400, RX35_03755, SAMEA3472043_04866, SAMEA3472044_04873, SAMEA3472047_03500, SAMEA3472055_04947, SAMEA3472056_05309, SAMEA3472070_05093, SAMEA3472080_04815, SAMEA3472090_05006, SAMEA3472108_04971, SAMEA3472110_05082, SAMEA3472112_05167, SAMEA3472114_04967, SAMEA3472147_04120, SAMEA3484427_04984, SAMEA3484429_04987, SAMEA3484434_04725, SAMEA3485101_05497, SAMEA3485113_04495, SAMEA3752372_05079, SAMEA3752553_04905, SAMEA3752557_04844, SAMEA3752559_04855, SAMEA3752620_04699, SAMEA3753064_05329, SAMEA3753097_04845, SAMEA3753164_04786, SAMEA3753290_00018, SAMEA3753300_04884, SK85_04319, UC41_25650, UN86_14180, UN91_25445, WQ89_24675, WR15_01490, YDC107_2702 Production host: ![]() ![]() References: UniProt: C3SIA2, UniProt: P0A8T7*PLUS, DNA-directed RNA polymerase |
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-DNA chain , 2 types, 2 molecules KL
#8: DNA chain | Mass: 15541.960 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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#9: DNA chain | Mass: 15211.717 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
-Protein / RNA chain , 2 types, 7 molecules fabcdeR
#10: RNA chain | Mass: 31693.904 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) / References: GenBank: 1227111474 |
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#2: Protein | Mass: 47070.168 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: rho, AC789_1c41660, ACN002_3874, EL75_4398, EL79_4648, EL80_4555 Production host: ![]() ![]() References: UniProt: A0A0A0GPI6, UniProt: P0AG30*PLUS, Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement |
-Non-polymers , 5 types, 33 molecules ![](data/chem/img/ADP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/BEF.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/BEF.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/HOH.gif)
#11: Chemical | ChemComp-ADP / #12: Chemical | ChemComp-MG / #13: Chemical | ChemComp-BEF / #14: Chemical | #15: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Transcription termination intermediate complex 2 / Type: COMPLEX / Entity ID: #1-#10 / Source: RECOMBINANT |
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Molecular weight | Value: 0.82 MDa / Experimental value: NO |
Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.6 |
Specimen | Conc.: 5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Tecnai Polara / Image courtesy: FEI Company |
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Microscopy | Model: FEI POLARA 300 |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 50 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||
Symmetry | Point symmetry: C1 (asymmetric) | ||||||||||||||||||||||||||||
3D reconstruction | Resolution: 5.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 8321 / Symmetry type: POINT |