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- PDB-6uze: Anthrax toxin protective antigen channels bound to edema factor -

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基本情報

登録情報
データベース: PDB / ID: 6uze
タイトルAnthrax toxin protective antigen channels bound to edema factor
要素
  • Calmodulin-sensitive adenylate cyclase
  • Protective antigen
キーワードTRANSLOCASE / anthrax toxin / protective antigen / edema factor
機能・相同性
機能・相同性情報


positive regulation of apoptotic process in another organism / calcium- and calmodulin-responsive adenylate cyclase activity / : / adenylate cyclase / cAMP biosynthetic process / adenylate cyclase activity / host cell cytosol / negative regulation of MAPK cascade / Uptake and function of anthrax toxins / catalytic complex ...positive regulation of apoptotic process in another organism / calcium- and calmodulin-responsive adenylate cyclase activity / : / adenylate cyclase / cAMP biosynthetic process / adenylate cyclase activity / host cell cytosol / negative regulation of MAPK cascade / Uptake and function of anthrax toxins / catalytic complex / small molecule binding / host cell endosome membrane / protein homooligomerization / metallopeptidase activity / toxin activity / calmodulin binding / host cell plasma membrane / extracellular region / ATP binding / identical protein binding / membrane / metal ion binding
類似検索 - 分子機能
: / Oedema factor (EF), alpha-helical domain / Protective antigen domain 4 / : / Anthrax protective antigen, immunoglobulin-like domain / Anthrax toxin, edema factor, central / Anthrax toxin, edema factor, C-terminal / Anthrax toxin, edema factor, central domain superfamily / Anthrax toxin LF subunit / Anthrax toxin, lethal/endema factor ...: / Oedema factor (EF), alpha-helical domain / Protective antigen domain 4 / : / Anthrax protective antigen, immunoglobulin-like domain / Anthrax toxin, edema factor, central / Anthrax toxin, edema factor, C-terminal / Anthrax toxin, edema factor, central domain superfamily / Anthrax toxin LF subunit / Anthrax toxin, lethal/endema factor / : / Anthrax toxin lethal factor (ATLF)-like domain profile. / Anthrax toxin, lethal/endema factor, N-/C-terminal / Anthrax toxin lethal factor, N- and C-terminal domain / Bacterial exotoxin B / Protective antigen, heptamerisation domain / Protective antigen, Ca-binding domain / Clostridial binary toxin B/anthrax toxin PA, domain 3 / Protective antigen, heptamerisation domain superfamily / Clostridial binary toxin B/anthrax toxin PA Ca-binding domain / Clostridial binary toxin B/anthrax toxin PA domain 2 / Clostridial binary toxin B/anthrax toxin PA domain 3 / PA14/GLEYA domain / PA14 domain profile. / PA14 domain / PA14 / PA14 domain / Metallopeptidase, catalytic domain superfamily
類似検索 - ドメイン・相同性
Protective antigen / Calmodulin-sensitive adenylate cyclase
類似検索 - 構成要素
生物種Bacillus anthracis (炭疽菌)
手法電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.4 Å
データ登録者Hardenbrook, N.J. / Liu, S. / Zhou, K. / Zhou, Z.H. / Krantz, B.A.
資金援助 米国, 3件
組織認可番号国
National Science Foundation (NSF, United States)DMR-1548924 米国
National Institutes of Health/National Center for Research Resources (NIH/NCRR)R01GM071940/AI094386/DE025567 米国
National Institutes of Health/National Center for Research Resources (NIH/NCRR)R21AI124020 米国
引用ジャーナル: Nat Commun / 年: 2020
タイトル: Atomic structures of anthrax toxin protective antigen channels bound to partially unfolded lethal and edema factors.
著者: Nathan J Hardenbrook / Shiheng Liu / Kang Zhou / Koyel Ghosal / Z Hong Zhou / Bryan A Krantz /
要旨: Following assembly, the anthrax protective antigen (PA) forms an oligomeric translocon that unfolds and translocates either its lethal factor (LF) or edema factor (EF) into the host cell. Here, we ...Following assembly, the anthrax protective antigen (PA) forms an oligomeric translocon that unfolds and translocates either its lethal factor (LF) or edema factor (EF) into the host cell. Here, we report the cryo-EM structures of heptameric PA channels with partially unfolded LF and EF at 4.6 and 3.1-Å resolution, respectively. The first α helix and β strand of LF and EF unfold and dock into a deep amphipathic cleft, called the α clamp, which resides at the interface of two PA monomers. The α-clamp-helix interactions exhibit structural plasticity when comparing the structures of lethal and edema toxins. EF undergoes a largescale conformational rearrangement when forming the complex with the channel. A critical loop in the PA binding interface is displaced for about 4 Å, leading to the weakening of the binding interface prior to translocation. These structures provide key insights into the molecular mechanisms of translocation-coupled protein unfolding and translocation.
履歴
登録2019年11月15日登録サイト: RCSB / 処理サイト: RCSB
改定 1.02020年3月4日Provider: repository / タイプ: Initial release
改定 1.12024年3月6日Group: Data collection / Database references / Derived calculations
カテゴリ: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id

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構造の表示

ムービー
  • 登録構造単位
  • Jmolによる作画
  • ダウンロード
  • EMマップとの重ね合わせ
  • マップデータ: EMDB-20958
  • UCSF Chimeraによる作画
  • ダウンロード
ムービービューア
構造ビューア分子:
MolmilJmol/JSmol

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集合体

登録構造単位
A: Protective antigen
B: Protective antigen
C: Protective antigen
D: Protective antigen
E: Protective antigen
F: Protective antigen
G: Protective antigen
H: Calmodulin-sensitive adenylate cyclase
I: Calmodulin-sensitive adenylate cyclase
ヘテロ分子


分子量 (理論値)分子数
合計 (水以外)757,85423
ポリマ－757,2939
非ポリマー56114
水00
1


  • 登録構造と同一
  • 登録者が定義した集合体
  • 根拠: assay for oligomerization
タイプ名称対称操作数
identity operation1_5551

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要素

#1: タンパク質
Protective antigen / PA / Anthrax toxins translocating protein / PA-83 / PA83


分子量: 82768.828 Da / 分子数: 7 / 由来タイプ: 組換発現 / 由来: (組換発現) Bacillus anthracis (炭疽菌) / 遺伝子: pagA, pag, pXO1-110, BXA0164, GBAA_pXO1_0164
発現宿主: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (大腸菌)
参照: UniProt: P13423
#2: タンパク質 Calmodulin-sensitive adenylate cyclase / ATP pyrophosphate-lyase / Adenylyl cyclase / Anthrax edema toxin adenylate cyclase component / ...ATP pyrophosphate-lyase / Adenylyl cyclase / Anthrax edema toxin adenylate cyclase component / Edema factor / EF


分子量: 88955.578 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) Bacillus anthracis (炭疽菌) / 遺伝子: cya, pXO1-122, BXA0141, GBAA_pXO1_0142
発現宿主: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (大腸菌)
参照: UniProt: P40136, adenylate cyclase
#3: 化合物
ChemComp-CA / CALCIUM ION / カルシウムジカチオン


分子量: 40.078 Da / 分子数: 14 / 由来タイプ: 合成 / 式: Ca
研究の焦点であるリガンドがあるかN

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実験情報

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実験

実験手法: 電子顕微鏡法
EM実験試料の集合状態: PARTICLE / ３次元再構成法: