+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6n89 | |||||||||
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タイトル | Cryo-EM structure of the Importin beta:Histone H1.0 complex | |||||||||
要素 |
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キーワード | TRANSPORT PROTEIN / Imp7:ImpB:H1.0 / Importin / Histone H1 / nuclear import / disordered interactions | |||||||||
機能・相同性 | 機能・相同性情報 RNA import into nucleus / Inhibition of nitric oxide production / mitotic chromosome movement towards spindle pole / endoplasmic reticulum tubular network / astral microtubule organization / establishment of mitotic spindle localization / positive regulation of transcription regulatory region DNA binding / negative regulation of DNA recombination / Transport of Ribonucleoproteins into the Host Nucleus / Regulation of cholesterol biosynthesis by SREBP (SREBF) ...RNA import into nucleus / Inhibition of nitric oxide production / mitotic chromosome movement towards spindle pole / endoplasmic reticulum tubular network / astral microtubule organization / establishment of mitotic spindle localization / positive regulation of transcription regulatory region DNA binding / negative regulation of DNA recombination / Transport of Ribonucleoproteins into the Host Nucleus / Regulation of cholesterol biosynthesis by SREBP (SREBF) / importin-alpha family protein binding / ribosomal protein import into nucleus / Initiation of Nuclear Envelope (NE) Reformation / NS1 Mediated Effects on Host Pathways / NLS-dependent protein nuclear import complex / Apoptosis induced DNA fragmentation / Nuclear import of Rev protein / chromosome condensation / Postmitotic nuclear pore complex (NPC) reformation / nuclear import signal receptor activity / Formation of Senescence-Associated Heterochromatin Foci (SAHF) / mitotic metaphase chromosome alignment / nuclear localization sequence binding / NLS-bearing protein import into nucleus / minor groove of adenine-thymine-rich DNA binding / nucleosome binding / mitotic spindle assembly / nuclear pore / nucleosomal DNA binding / transcription repressor complex / Assembly of the ORC complex at the origin of replication / Hsp90 protein binding / euchromatin / heterochromatin formation / chromatin DNA binding / ISG15 antiviral mechanism / small GTPase binding / cytoplasmic stress granule / specific granule lumen / protein import into nucleus / structural constituent of chromatin / SARS-CoV-1 activates/modulates innate immune responses / Interferon alpha/beta signaling / nucleosome / actin cytoskeleton / nucleosome assembly / nuclear envelope / double-stranded DNA binding / nuclear membrane / ficolin-1-rich granule lumen / nuclear body / protein domain specific binding / Neutrophil degranulation / chromatin / Golgi apparatus / enzyme binding / RNA binding / extracellular exosome / zinc ion binding / extracellular region / nucleoplasm / membrane / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | Homo sapiens (ヒト) | |||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 7.5 Å | |||||||||
データ登録者 | Bilokapic, S. / Ivic, N. / Halic, M. | |||||||||
資金援助 | European Union, クロアチア, 2件
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引用 | ジャーナル: Mol Cell / 年: 2019 タイトル: Fuzzy Interactions Form and Shape the Histone Transport Complex. 著者: Nives Ivic / Mia Potocnjak / Victor Solis-Mezarino / Franz Herzog / Silvija Bilokapic / Mario Halic / 要旨: Protein transport into the nucleus is mediated by transport receptors. Import of highly charged proteins, such as histone H1 and ribosomal proteins, requires a dimer of two transport receptors. In ...Protein transport into the nucleus is mediated by transport receptors. Import of highly charged proteins, such as histone H1 and ribosomal proteins, requires a dimer of two transport receptors. In this study, we determined the cryo-EM structure of the Imp7:Impβ:H1.0 complex, showing that the two importins form a cradle that accommodates the linker histone. The H1.0 globular domain is bound to Impβ, whereas the acidic loops of Impβ and Imp7 chaperone the positively charged C-terminal tail. Although it remains disordered, the H1 tail serves as a zipper that closes and stabilizes the structure through transient non-specific interactions with importins. Moreover, we found that the GGxxF and FxFG motifs in the Imp7 C-terminal tail are essential for Imp7:Impβ dimerization and H1 import, resembling importin interaction with nucleoporins, which, in turn, promote complex disassembly. The architecture of many other complexes might be similarly defined by rapidly exchanging electrostatic interactions mediated by disordered regions. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6n89.cif.gz | 132.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6n89.ent.gz | 80.8 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6n89.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 6n89_validation.pdf.gz | 730.9 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 6n89_full_validation.pdf.gz | 734.5 KB | 表示 | |
XML形式データ | 6n89_validation.xml.gz | 25 KB | 表示 | |
CIF形式データ | 6n89_validation.cif.gz | 39.7 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/n8/6n89 ftp://data.pdbj.org/pub/pdb/validation_reports/n8/6n89 | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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-要素
#1: タンパク質 | 分子量: 97257.812 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: KPNB1, NTF97 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q14974 |
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#2: タンパク質 | 分子量: 20927.182 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: H1F0, H1FV / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P07305 |
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: Imp7:ImpB:H1.0 / タイプ: COMPLEX / Entity ID: all / 由来: RECOMBINANT |
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由来(天然) | 生物種: Homo sapiens (ヒト) |
由来(組換発現) | 生物種: Escherichia coli (大腸菌) |
緩衝液 | pH: 7 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
試料支持 | 詳細: unspecified |
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 80 e/Å2 フィルム・検出器のモデル: GATAN K2 BASE (4k x 4k) |
-解析
ソフトウェア | 名称: PHENIX / バージョン: 1.11.1_2575: / 分類: 精密化 | ||||||||||||||||||||||||
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3次元再構成 | 解像度: 7.5 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 15800 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
拘束条件 |
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