登録情報 データベース : PDB / ID : 6foo 構造の表示 ダウンロードとリンクタイトル Structure of Ryanodine Receptor 1 in nanodiscs in the presence of calcium and ATP 要素Ryanodine receptor 1 詳細 キーワード MEMBRANE PROTEIN / calcium release channel / nanodiscs機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
ATP-gated ion channel activity / terminal cisterna / ryanodine receptor complex / ryanodine-sensitive calcium-release channel activity / release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / ossification involved in bone maturation / skin development / cellular response to caffeine / outflow tract morphogenesis / intracellularly gated calcium channel activity ... ATP-gated ion channel activity / terminal cisterna / ryanodine receptor complex / ryanodine-sensitive calcium-release channel activity / release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / ossification involved in bone maturation / skin development / cellular response to caffeine / outflow tract morphogenesis / intracellularly gated calcium channel activity / organelle membrane / toxic substance binding / smooth endoplasmic reticulum / voltage-gated calcium channel activity / skeletal muscle fiber development / striated muscle contraction / release of sequestered calcium ion into cytosol / sarcoplasmic reticulum membrane / cellular response to calcium ion / sarcoplasmic reticulum / muscle contraction / calcium ion transmembrane transport / calcium channel activity / sarcolemma / Z disc / intracellular calcium ion homeostasis / disordered domain specific binding / protein homotetramerization / transmembrane transporter binding / calmodulin binding / calcium ion binding / ATP binding / identical protein binding / membrane 類似検索 - 分子機能 : / Ryanodine receptor junctional solenoid repeat / Ryanodine receptor, SPRY domain 2 / Ryanodine Receptor TM 4-6 / Ryanodine receptor / Ryanodine receptor, SPRY domain 1 / Ryanodine receptor, SPRY domain 3 / Ryanodine Receptor TM 4-6 / Ryanodine receptor Ryr / RyR domain ... : / Ryanodine receptor junctional solenoid repeat / Ryanodine receptor, SPRY domain 2 / Ryanodine Receptor TM 4-6 / Ryanodine receptor / Ryanodine receptor, SPRY domain 1 / Ryanodine receptor, SPRY domain 3 / Ryanodine Receptor TM 4-6 / Ryanodine receptor Ryr / RyR domain / RyR/IP3 receptor binding core, RIH domain superfamily / : / RyR/IP3R Homology associated domain / Inositol 1,4,5-trisphosphate/ryanodine receptor / RIH domain / RyR and IP3R Homology associated / Inositol 1,4,5-trisphosphate/ryanodine receptor / RIH domain / MIR motif / MIR domain / MIR domain profile. / Domain in ryanodine and inositol trisphosphate receptors and protein O-mannosyltransferases / Mir domain superfamily / SPRY domain / B30.2/SPRY domain / B30.2/SPRY domain profile. / SPRY domain / B30.2/SPRY domain superfamily / Domain in SPla and the RYanodine Receptor. / Ion transport domain / Ion transport protein / EF-hand domain pair / Concanavalin A-like lectin/glucanase domain superfamily 類似検索 - ドメイン・相同性生物種 Oryctolagus cuniculus (ウサギ)手法 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 8.2 Å 詳細データ登録者 Willegems, K. / Efremov, R.G. 資金援助 ベルギー, 2件 詳細 詳細を隠す組織 認可番号 国 IWT 131261 ベルギー FWO G.0266.15N ベルギー
引用ジャーナル : Structure / 年 : 2018タイトル : Influence of Lipid Mimetics on Gating of Ryanodine Receptor.著者 : Katrien Willegems / Rouslan G Efremov / 要旨 : Understanding gating principles of ion channels at high resolution is of great importance. Here we investigate the conformational transition from closed to open state in ryanodine receptor 1 (RyR1) ... Understanding gating principles of ion channels at high resolution is of great importance. Here we investigate the conformational transition from closed to open state in ryanodine receptor 1 (RyR1) reconstituted into lipid nanodiscs. RyR1 is a homotetrameric giant ion channel that couples excitation of muscle cells to fast calcium release from the sarcoplasmic reticulum. Using single-particle cryo-EM we show that RyR1 reconstituted into lipid nanodiscs is stabilized in the open conformation when bound to the plant toxin ryanodine, but not in the presence of its physiological activators, calcium and ATP. Further, using ryanodine binding assays we show that membrane mimetics influence RyR1 transition between closed and open-channel conformations. We find that all detergents, including fluorinated detergents added to nanodiscs, stabilize closed state of RyR1. Our biochemical results correlate with available structural data and suggest optimal conditions for structural studies of RyR1 gating. 履歴 登録 2018年2月8日 登録サイト : PDBE / 処理サイト : PDBE改定 1.0 2018年8月8日 Provider : repository / タイプ : Initial release改定 1.1 2018年8月15日 Group : Data collection / Database references / Structure summaryカテゴリ : citation / entity / entity_name_comItem : _citation.journal_id_ISSN / _citation.pdbx_database_id_PubMed ... _citation.journal_id_ISSN / _citation.pdbx_database_id_PubMed / _citation.title / _entity.pdbx_description / _entity_name_com.name 改定 1.2 2018年8月29日 Group : Data collection / Database references / カテゴリ : pdbx_database_related / Item : _pdbx_database_related.db_id改定 1.3 2018年10月10日 Group : Data collection / Database references / Structure summaryカテゴリ : citation / entityItem : _citation.journal_volume / _citation.page_first ... _citation.journal_volume / _citation.page_first / _citation.page_last / _entity.formula_weight 改定 1.4 2018年11月28日 Group : Data collection / Database references / Structure summaryカテゴリ : entity / pdbx_database_relatedItem : _entity.formula_weight / _pdbx_database_related.content_type改定 1.5 2019年11月6日 Group : Data collection / Refinement description / カテゴリ : software / Item : _software.name改定 1.6 2024年5月15日 Group : Data collection / Database references / カテゴリ : chem_comp_atom / chem_comp_bond / database_2Item : _database_2.pdbx_DOI / _database_2.pdbx_database_accession
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