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- PDB-6c7j: Crystal structure of human phosphodiesterase 2A with 1-(5-tert-bu... -

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Basic information

Entry
Database: PDB / ID: 6c7j
TitleCrystal structure of human phosphodiesterase 2A with 1-(5-tert-butoxy-2-chloro-phenyl)-N-isobutyl-4-methyl-[1,2,4]triazolo[4,3-a]quinoxaline-8-carboxamide
ComponentscGMP-dependent 3',5'-cyclic phosphodiesterase
KeywordsHYDROLASE / phosphodiesterase
Function / homology
Function and homology information


regulation of cGMP-mediated signaling / cellular response to 2,3,7,8-tetrachlorodibenzodioxine / cellular response to macrophage colony-stimulating factor stimulus / cellular response to cGMP / negative regulation of cAMP-mediated signaling / negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / positive regulation of vascular permeability / heart valve development / cellular response to granulocyte macrophage colony-stimulating factor stimulus / negative regulation of vascular permeability ...regulation of cGMP-mediated signaling / cellular response to 2,3,7,8-tetrachlorodibenzodioxine / cellular response to macrophage colony-stimulating factor stimulus / cellular response to cGMP / negative regulation of cAMP-mediated signaling / negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway / positive regulation of vascular permeability / heart valve development / cellular response to granulocyte macrophage colony-stimulating factor stimulus / negative regulation of vascular permeability / regulation of mitochondrion organization / establishment of endothelial barrier / aorta development / cGMP-mediated signaling / ventricular septum development / 3',5'-cGMP-stimulated cyclic-nucleotide phosphodiesterase activity / 3',5'-cyclic-nucleotide phosphodiesterase / negative regulation of cGMP-mediated signaling / TPR domain binding / cGMP catabolic process / phosphate ion binding / cGMP effects / monocyte differentiation / cGMP binding / 3',5'-cyclic-GMP phosphodiesterase activity / 3',5'-cyclic-AMP phosphodiesterase activity / regulation of cAMP-mediated signaling / cAMP binding / cellular response to transforming growth factor beta stimulus / cellular response to cAMP / cAMP-mediated signaling / adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway / synaptic membrane / cellular response to mechanical stimulus / positive regulation of inflammatory response / cellular response to xenobiotic stimulus / presynaptic membrane / G alpha (s) signalling events / mitochondrial outer membrane / mitochondrial inner membrane / mitochondrial matrix / positive regulation of gene expression / perinuclear region of cytoplasm / Golgi apparatus / negative regulation of transcription by RNA polymerase II / magnesium ion binding / endoplasmic reticulum / protein homodimerization activity / zinc ion binding / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Catalytic domain of cyclic nucleotide phosphodiesterase 4b2b / 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain / 3'5'-cyclic nucleotide phosphodiesterase / 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain / 3'5'-cyclic nucleotide phosphodiesterase, conserved site / 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain superfamily / 3'5'-cyclic nucleotide phosphodiesterase / 3'5'-cyclic nucleotide phosphodiesterase domain signature. / 3'5'-cyclic nucleotide phosphodiesterase domain profile. / GAF domain ...Catalytic domain of cyclic nucleotide phosphodiesterase 4b2b / 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain / 3'5'-cyclic nucleotide phosphodiesterase / 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain / 3'5'-cyclic nucleotide phosphodiesterase, conserved site / 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain superfamily / 3'5'-cyclic nucleotide phosphodiesterase / 3'5'-cyclic nucleotide phosphodiesterase domain signature. / 3'5'-cyclic nucleotide phosphodiesterase domain profile. / GAF domain / Domain present in phytochromes and cGMP-specific phosphodiesterases. / GAF domain / GAF-like domain superfamily / Metal dependent phosphohydrolases with conserved 'HD' motif. / HD/PDEase domain / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Chem-EPV / cGMP-dependent 3',5'-cyclic phosphodiesterase
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 1.85 Å
AuthorsXu, R. / Aertgeerts, K.
CitationJournal: J. Med. Chem. / Year: 2018
Title: Mathematical and Structural Characterization of Strong Nonadditive Structure-Activity Relationship Caused by Protein Conformational Changes.
Authors: Gomez, L. / Xu, R. / Sinko, W. / Selfridge, B. / Vernier, W. / Ly, K. / Truong, R. / Metz, M. / Marrone, T. / Sebring, K. / Yan, Y. / Appleton, B. / Aertgeerts, K. / Massari, M.E. / Breitenbucher, J.G.
History
DepositionJan 22, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 15, 2018Provider: repository / Type: Initial release
Revision 1.1Aug 29, 2018Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.title / _citation_author.identifier_ORCID / _citation_author.name
Revision 1.2Sep 26, 2018Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.3Mar 13, 2024Group: Data collection / Database references / Derived calculations
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_conn_angle / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: cGMP-dependent 3',5'-cyclic phosphodiesterase
B: cGMP-dependent 3',5'-cyclic phosphodiesterase
C: cGMP-dependent 3',5'-cyclic phosphodiesterase
D: cGMP-dependent 3',5'-cyclic phosphodiesterase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)161,86216
Polymers159,6394
Non-polymers2,22312
Water16,304905
1
A: cGMP-dependent 3',5'-cyclic phosphodiesterase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,4664
Polymers39,9101
Non-polymers5563
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: cGMP-dependent 3',5'-cyclic phosphodiesterase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,4664
Polymers39,9101
Non-polymers5563
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: cGMP-dependent 3',5'-cyclic phosphodiesterase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,4664
Polymers39,9101
Non-polymers5563
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: cGMP-dependent 3',5'-cyclic phosphodiesterase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,4664
Polymers39,9101
Non-polymers5563
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)55.930, 73.450, 91.030
Angle α, β, γ (deg.)109.120, 91.250, 91.560
Int Tables number1
Space group name H-MP1

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Components

#1: Protein
cGMP-dependent 3',5'-cyclic phosphodiesterase / Cyclic GMP-stimulated phosphodiesterase / cGSPDE


Mass: 39909.824 Da / Num. of mol.: 4 / Fragment: phosphodiesterase 2A (UNP residues 323-661)
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PDE2A / Plasmid: pFastbac-HT / Production host: Spodoptera frugiperda (fall armyworm) / Strain (production host): Sf9
References: UniProt: O00408, 3',5'-cyclic-nucleotide phosphodiesterase
#2: Chemical
ChemComp-EPV / 1-(5-tert-butoxy-2-chlorophenyl)-4-methyl-N-(2-methylpropyl)[1,2,4]triazolo[4,3-a]quinoxaline-8-carboxamide


Mass: 465.975 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C25H28ClN5O2
#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn
#4: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 905 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.21 Å3/Da / Density % sol: 44.38 % / Mosaicity: 0 °
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.4
Details: 17-19% PEG3350, 0.2 M magnesium chloride, 0.1 M Tris, pH 8.4

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 5.0.1 / Wavelength: 0.97741 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 19, 2016
RadiationMonochromator: Single crystal, cylindrically bent, Si(220) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97741 Å / Relative weight: 1
ReflectionResolution: 1.85→55.88 Å / Num. obs: 111170 / % possible obs: 95.2 % / Redundancy: 2 % / Biso Wilson estimate: 22.26 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.07 / Rpim(I) all: 0.07 / Rrim(I) all: 0.099 / Net I/σ(I): 10.2 / Num. measured all: 218706
Reflection shell

Diffraction-ID: 1 / Redundancy: 2 %

Resolution (Å)Rmerge(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) all% possible all
1.85-1.90.82181260.4910.8211.16194.2
8.27-55.880.01412370.9990.0140.0295

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
Aimless0.5.23data scaling
PHASERphasing
PHENIXrefinement
PDB_EXTRACT3.24data extraction
DIALSdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.85→55.88 Å / SU ML: 0.26 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 24.63
RfactorNum. reflection% reflection
Rfree0.2302 5562 5 %
Rwork0.1761 --
obs0.1787 111152 95.2 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 116.18 Å2 / Biso mean: 29.2075 Å2 / Biso min: 9 Å2
Refinement stepCycle: final / Resolution: 1.85→55.88 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11003 0 140 905 12048
Biso mean--23.06 32.4 -
Num. residues----1345
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00711413
X-RAY DIFFRACTIONf_angle_d1.0515420
X-RAY DIFFRACTIONf_chiral_restr0.0391639
X-RAY DIFFRACTIONf_plane_restr0.0051999
X-RAY DIFFRACTIONf_dihedral_angle_d13.8864224
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
1.85-1.8710.3521730.30563481365494
1.871-1.89310.36531800.29823490367094
1.8931-1.91610.35071980.29753435363394
1.9161-1.94040.34832040.28733482368694
1.9404-1.96590.32271760.26453489366595
1.9659-1.99290.31561920.24853561375395
1.9929-2.02130.31931940.24543447364195
2.0213-2.05150.281730.243513368695
2.0515-2.08360.29751820.22673476365894
2.0836-2.11770.28821660.2193487365395
2.1177-2.15430.24371760.2073603377995
2.1543-2.19340.25691810.19463457363895
2.1934-2.23560.25061720.19233579375195
2.2356-2.28120.26171840.19953473365794
2.2812-2.33090.2341610.17663587374896
2.3309-2.38510.23551900.1783480367095
2.3851-2.44470.2442240.17263521374596
2.4447-2.51080.25232020.17393519372196
2.5108-2.58470.23132330.1663503373696
2.5847-2.66810.23021670.16923595376296
2.6681-2.76350.2111900.17133526371696
2.7635-2.87410.22752130.16723502371596
2.8741-3.00490.19751850.16443567375296
3.0049-3.16330.21281880.1563557374596
3.1633-3.36150.22441830.15423596377997
3.3615-3.6210.19261790.15273518369796
3.621-3.98530.19982040.13933495369995
3.9853-4.56180.18151190.13853577369695
4.5618-5.74640.18441780.14963532371096
5.7464-55.90510.1931950.16173542373796
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.8711.28221.08953.60411.47191.76210.0721-0.216-0.15610.1621-0.0340.02140.257-0.0681-0.01360.2291-0.03170.03270.22840.03940.229-8.2512-16.05243.3071
22.49170.10161.59981.4934-0.71435.10770.1960.0739-0.1739-0.0679-0.0390.21340.2377-0.4081-0.20040.1654-0.05160.01670.19690.00170.2211-12.2909-11.9899-4.4168
36.08022.17582.69032.13531.19022.72970.09010.0074-0.4846-0.03450.0804-0.08770.1462-0.0182-0.23820.16550.01090.00620.09190.02130.16231.8765-12.6351-4.6985
42.59050.23480.1141.08670.14981.4712-0.01270.06260.0219-0.08610.0090.0418-0.02030.0124-0.00070.1365-0.0185-0.00960.08210.00690.11660.1378-2.4555-7.8819
54.95781.05492.80383.33031.88866.2173-0.37010.6650.3453-0.68790.1760.3457-0.3393-0.02950.18510.2456-0.0187-0.0170.24270.05710.1693-4.66381.405-16.2593
63.1104-3.7553-0.30125.34310.12373.8437-0.07510.13240.8268-0.05710.0139-0.1135-0.7240.1923-0.01080.3158-0.0307-0.020.163-0.02550.2901-1.347417.1571-3.9551
75.4727-2.48343.61125.1741-3.38286.9051-0.04110.04470.3122-0.13010.03460.2367-0.2169-0.16530.04140.1126-0.0055-0.00060.1096-0.02390.18-7.64588.9864-3.0408
82.2902-0.7095-2.86881.45881.01874.1849-0.0023-0.7620.09490.1350.0936-0.15040.19580.5656-0.01390.18420.0068-0.05080.2930.02210.161514.3475-0.76459.2002
94.1127-0.87590.76271.6910.15412.4142-0.0532-0.4170.59610.13120.0256-0.1253-0.22270.17280.03240.2107-0.0136-0.00920.1361-0.03670.20048.86059.97258.5159
108.7308-1.67645.8920.5151-1.16794.52160.0465-0.3238-0.10030.19070.05170.0099-0.1071-0.1258-0.03350.3240.01380.0250.3486-0.10050.24229.0825.577622.0986
111.3439-0.96121.04323.4138-1.51083.17370.09890.0861-0.3584-0.1958-0.0405-0.08790.61660.2384-0.03290.33380.0798-0.01110.1948-0.03560.253125.2413-19.8323-40.87
122.7744-0.96830.45433.2957-1.33134.2421-0.0422-0.4324-0.16880.2194-0.0838-0.58730.25860.56370.02650.27590.1034-0.01280.3118-0.0030.313530.2717-15.5109-33.7172
134.4593-1.28581.29052.6065-0.35312.9346-0.0943-0.1717-0.30120.12660.1129-0.05150.3178-0.0448-0.05640.2177-0.00170.01240.1065-0.00220.157516.4154-14.1945-31.3118
143.0364-0.1532-0.09431.71660.37242.24890.0085-0.03610.0489-0.02130.0107-0.0982-0.02780.0747-0.01180.16170.0104-0.0050.07040.00810.11120.2627-3.3352-34.4688
155.8576-0.8834-0.45024.72361.94555.6646-0.0675-0.42370.36190.61120.09770.4208-0.401-0.25920.00750.20060.01850.05550.1706-0.02490.166313.1092-0.3443-24.5408
163.32120.22741.61841.63440.82713.9268-0.0427-0.2371-0.15190.35880.0167-0.23920.07780.33140.030.19380.01770.01740.18940.00570.151126.5445-4.1459-21.9833
172.94091.22840.48062.19070.59171.83580.054-0.05990.2916-0.10080.0645-0.157-0.32750.0916-0.08350.21910.00080.04050.1080.01610.193625.53558.6968-38.3489
183.4061-1.8087-3.89212.52711.37745.0712-0.00360.3127-0.3563-0.1013-0.25020.27730.0362-0.37290.21570.21960.0141-0.0710.3296-0.03130.18012.9336-3.4387-45.0379
194.3450.99310.37161.29950.32471.9409-0.06460.29770.5562-0.2350.01350.1793-0.3529-0.31980.00240.29020.0546-0.01570.19870.03870.19119.57936.3961-47.3615
209.31522.0445.63670.69151.17433.3565-0.12050.2899-0.1358-0.26070.15190.0342-0.10530.0457-0.01610.40620.02980.02240.38320.04620.20867.89-0.5571-59.6875
211.03770.28270.37191.5433-0.33642.17910.03140.3009-0.3219-0.1693-0.0786-0.41350.44720.77160.06760.27160.13780.01470.4118-0.04730.306354.3896-45.34-36.7691
223.5402-0.23320.70090.97750.02182.34640.08780.11020.00270.054-0.0025-0.1324-0.0280.278-0.0870.17-0.0064-0.00290.1214-0.01940.143945.7189-37.2618-25.2521
233.1590.48390.77741.64680.28023.59950.20950.11480.1535-0.0727-0.13510.0487-0.3748-0.2524-0.07990.25550.04840.04420.2453-0.00520.157839.8422-30.0155-41.4498
245.12973.04034.14482.0831.96163.9308-0.28210.15740.0373-0.30680.19730.0095-0.24270.26860.04630.34450.06160.04180.4405-0.01620.216334.9504-35.9027-59.4156
252.37761.00550.62623.1031-0.14151.31980.1623-0.3905-0.51120.1937-0.14890.38140.5617-0.5651-0.02180.3903-0.15690.03790.44350.08890.342915.5527-45.35479.4135
261.28050.59710.28881.50340.86461.78160.1993-0.266-0.28410.1128-0.1950.2350.3213-0.39920.00370.2231-0.0767-0.01870.21350.0530.249819.8258-42.63521.0427
274.2738-2.37411.01787.65250.60793.16680.175-0.1843-0.244-0.1266-0.22840.627-0.0443-0.40220.06660.1128-0.03780.00260.2031-0.00180.188915.5826-30.7592-3.3368
285.19171.01930.68251.15850.1481.08930.05870.1216-0.2028-0.09230.0238-0.01290.10040.07-0.08330.17190.0081-0.02580.10580.01020.140833.7958-38.9927-9.7878
299.06013.39936.1954.93773.03687.4614-0.18660.6470.3092-0.37580.18050.1812-0.41830.0350.05230.17980.01730.00940.18820.06210.204521.9081-32.9047-13.6263
305.6979-5.47620.96165.2764-0.92810.15650.15310.44830.6057-0.1577-0.196-0.5016-0.00960.07150.01870.20260.00690.03060.25410.01180.277629.1735-17.287-2.7922
314.1012-3.30123.49026.1677-4.86975.81610.1255-0.0703-0.0613-0.19070.06640.21240.0564-0.1104-0.17660.0948-0.01970.02090.1639-0.00730.12721.3763-23.8692-0.9957
328.548-1.8574-3.20272.05571.30923.2846-0.002-0.541-0.37760.1749-0.0006-0.01660.33530.35520.01850.2161-0.0091-0.080.26020.05860.18242.0561-36.221610.9811
334.3107-2.13571.03622.54120.0572.2773-0.11-0.51990.14710.14980.1848-0.2121-0.02240.2061-0.0810.1683-0.0324-0.00060.20670.02720.158838.1007-24.72659.7372
349.3516-3.11476.26371.7003-1.8915.19350.0163-0.2692-0.11530.19470.0471-0.00370.1134-0.1844-0.01730.2829-0.01120.02840.3245-0.02240.2238.2558-28.304423.5138
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 576 through 611 )A576 - 611
2X-RAY DIFFRACTION2chain 'A' and (resid 612 through 635 )A612 - 635
3X-RAY DIFFRACTION3chain 'A' and (resid 636 through 657 )A636 - 657
4X-RAY DIFFRACTION4chain 'A' and (resid 658 through 750 )A658 - 750
5X-RAY DIFFRACTION5chain 'A' and (resid 751 through 768 )A751 - 768
6X-RAY DIFFRACTION6chain 'A' and (resid 769 through 786 )A769 - 786
7X-RAY DIFFRACTION7chain 'A' and (resid 787 through 815 )A787 - 815
8X-RAY DIFFRACTION8chain 'A' and (resid 816 through 840 )A816 - 840
9X-RAY DIFFRACTION9chain 'A' and (resid 841 through 878 )A841 - 878
10X-RAY DIFFRACTION10chain 'A' and (resid 879 through 917 )A879 - 917
11X-RAY DIFFRACTION11chain 'B' and (resid 578 through 611 )B578 - 611
12X-RAY DIFFRACTION12chain 'B' and (resid 612 through 635 )B612 - 635
13X-RAY DIFFRACTION13chain 'B' and (resid 636 through 657 )B636 - 657
14X-RAY DIFFRACTION14chain 'B' and (resid 658 through 718 )B658 - 718
15X-RAY DIFFRACTION15chain 'B' and (resid 719 through 738 )B719 - 738
16X-RAY DIFFRACTION16chain 'B' and (resid 739 through 768 )B739 - 768
17X-RAY DIFFRACTION17chain 'B' and (resid 769 through 815 )B769 - 815
18X-RAY DIFFRACTION18chain 'B' and (resid 816 through 840 )B816 - 840
19X-RAY DIFFRACTION19chain 'B' and (resid 841 through 878 )B841 - 878
20X-RAY DIFFRACTION20chain 'B' and (resid 879 through 916 )B879 - 916
21X-RAY DIFFRACTION21chain 'C' and (resid 590 through 675 )C590 - 675
22X-RAY DIFFRACTION22chain 'C' and (resid 676 through 768 )C676 - 768
23X-RAY DIFFRACTION23chain 'C' and (resid 769 through 878 )C769 - 878
24X-RAY DIFFRACTION24chain 'C' and (resid 879 through 917 )C879 - 917
25X-RAY DIFFRACTION25chain 'D' and (resid 582 through 611 )D582 - 611
26X-RAY DIFFRACTION26chain 'D' and (resid 612 through 674 )D612 - 674
27X-RAY DIFFRACTION27chain 'D' and (resid 675 through 695 )D675 - 695
28X-RAY DIFFRACTION28chain 'D' and (resid 696 through 750 )D696 - 750
29X-RAY DIFFRACTION29chain 'D' and (resid 751 through 768 )D751 - 768
30X-RAY DIFFRACTION30chain 'D' and (resid 769 through 786 )D769 - 786
31X-RAY DIFFRACTION31chain 'D' and (resid 787 through 815 )D787 - 815
32X-RAY DIFFRACTION32chain 'D' and (resid 816 through 840 )D816 - 840
33X-RAY DIFFRACTION33chain 'D' and (resid 841 through 878 )D841 - 878
34X-RAY DIFFRACTION34chain 'D' and (resid 879 through 917 )D879 - 917

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