+
Open data
-
Basic information
Entry | Database: PDB / ID: 6bab | ||||||
---|---|---|---|---|---|---|---|
Title | The structure of human CamKII with bound inhibitor | ||||||
![]() | Calcium/calmodulin-dependent protein kinase type II subunit delta | ||||||
![]() | TRANSFERASE/TRANSFERASE Inhibitor / Kinase / CamKII / inhibitor / TRANSFERASE / TRANSFERASE-TRANSFERASE Inhibitor complex | ||||||
Function / homology | ![]() Phase 0 - rapid depolarisation / regulation of relaxation of cardiac muscle / HSF1-dependent transactivation / Interferon gamma signaling / Ion transport by P-type ATPases / regulation of cellular localization / negative regulation of sodium ion transmembrane transport / Unblocking of NMDA receptors, glutamate binding and activation / Trafficking of AMPA receptors / RAF activation ...Phase 0 - rapid depolarisation / regulation of relaxation of cardiac muscle / HSF1-dependent transactivation / Interferon gamma signaling / Ion transport by P-type ATPases / regulation of cellular localization / negative regulation of sodium ion transmembrane transport / Unblocking of NMDA receptors, glutamate binding and activation / Trafficking of AMPA receptors / RAF activation / protein serine/threonine kinase activity => GO:0004674 / regulation of cell communication by electrical coupling / cell growth involved in cardiac muscle cell development / calcium- and calmodulin-dependent protein kinase complex / RAF/MAP kinase cascade / negative regulation of sodium ion transmembrane transporter activity / Ca2+/calmodulin-dependent protein kinase / axon initial segment / regulation of membrane depolarization / intracellular potassium ion homeostasis / Ion homeostasis / cardiac muscle cell contraction / endoplasmic reticulum calcium ion homeostasis / sodium channel inhibitor activity / relaxation of cardiac muscle / regulation of calcium ion transmembrane transport via high voltage-gated calcium channel / calcium/calmodulin-dependent protein kinase activity / regulation of cardiac muscle cell action potential / positive regulation of DNA biosynthetic process / nitric-oxide synthase binding / positive regulation of cardiac muscle hypertrophy / positive regulation of cardiac muscle cell apoptotic process / positive regulation of smooth muscle cell migration / positive regulation of Rac protein signal transduction / intercalated disc / regulation of protein localization to plasma membrane / regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / cardiac muscle contraction / regulation of sodium ion transport / titin binding / positive regulation of vascular associated smooth muscle cell proliferation / T-tubule / sarcoplasmic reticulum membrane / positive regulation of G2/M transition of mitotic cell cycle / sarcoplasmic reticulum / peptidyl-threonine phosphorylation / neuromuscular junction / G1/S transition of mitotic cell cycle / calcium ion transport / peptidyl-serine phosphorylation / transmembrane transporter binding / protein autophosphorylation / positive regulation of ERK1 and ERK2 cascade / calmodulin binding / response to hypoxia / protein kinase activity / neuron projection / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / neuronal cell body / perinuclear region of cytoplasm / protein homodimerization activity / protein-containing complex / ATP binding / identical protein binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Somoza, J.R. / Villasenor, A.G. | ||||||
![]() | ![]() Title: TBD Authors: Koltun, D. / Somoza, J.R. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 256.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 215.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 53.4 KB | Display | |
Data in CIF | ![]() | 76.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||||
2 | ![]()
| ||||||||||
3 | ![]()
| ||||||||||
4 | ![]()
| ||||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 34459.574 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q6PHZ2, Ca2+/calmodulin-dependent protein kinase #2: Chemical | ChemComp-D0S / #3: Chemical | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.58 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion Details: Sitting drop vapor diffusion droplets were assembled with 250 nL of 12 mg/mL CamKII, 0.6 mM inhibitor and 250 nL of reservoir solution 24% peg 3350, 0.2 M ammonium tartrate, 0.1 M arginine. |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Feb 21, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 1.91→54.2 Å / Num. obs: 80037 / % possible obs: 87.4 % / Redundancy: 2 % / Biso Wilson estimate: 22.25 Å2 / Net I/σ(I): 7.6 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Stereochemistry target values: GEOSTD + MONOMER LIBRARY + CDL V1.2
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.33 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.91→54.18 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
|