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Yorodumi- PDB-5zji: Structure of photosystem I supercomplex with light-harvesting com... -
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-Basic information
Entry | Database: PDB / ID: 5zji | |||||||||||||||||||||||||||||||||
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Title | Structure of photosystem I supercomplex with light-harvesting complexes I and II | |||||||||||||||||||||||||||||||||
Components |
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Keywords | MEMBRANE PROTEIN / Photosystem / Antenna / Supercomplex / State transition | |||||||||||||||||||||||||||||||||
Function / homology | Function and homology information plastoglobule / thylakoid membrane / photosynthesis, light harvesting in photosystem I / photosynthesis, light harvesting / chloroplast envelope / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / : / photosystem I ...plastoglobule / thylakoid membrane / photosynthesis, light harvesting in photosystem I / photosynthesis, light harvesting / chloroplast envelope / photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / : / photosystem I / photosystem II / plastid / chlorophyll binding / chloroplast thylakoid membrane / response to light stimulus / photosynthesis / chloroplast / 4 iron, 4 sulfur cluster binding / electron transfer activity / membrane => GO:0016020 / magnesium ion binding / metal ion binding Similarity search - Function | |||||||||||||||||||||||||||||||||
Biological species | Zea mays (maize) Zea mays subsp. mays (maize) | |||||||||||||||||||||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.3 Å | |||||||||||||||||||||||||||||||||
Authors | Pan, X.W. / Ma, J. / Su, X.D. / Cao, P. / Liu, Z.F. / Zhang, X.Z. / Li, M. | |||||||||||||||||||||||||||||||||
Funding support | China, 10items
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Citation | Journal: Science / Year: 2018 Title: Structure of the maize photosystem I supercomplex with light-harvesting complexes I and II. Authors: Xiaowei Pan / Jun Ma / Xiaodong Su / Peng Cao / Wenrui Chang / Zhenfeng Liu / Xinzheng Zhang / Mei Li / Abstract: Plants regulate photosynthetic light harvesting to maintain balanced energy flux into photosystems I and II (PSI and PSII). Under light conditions favoring PSII excitation, the PSII antenna, light- ...Plants regulate photosynthetic light harvesting to maintain balanced energy flux into photosystems I and II (PSI and PSII). Under light conditions favoring PSII excitation, the PSII antenna, light-harvesting complex II (LHCII), is phosphorylated and forms a supercomplex with PSI core and the PSI antenna, light-harvesting complex I (LHCI). Both LHCI and LHCII then transfer excitation energy to the PSI core. We report the structure of maize PSI-LHCI-LHCII solved by cryo-electron microscopy, revealing the recognition site between LHCII and PSI. The PSI subunits PsaN and PsaO are observed at the PSI-LHCI interface and the PSI-LHCII interface, respectively. Each subunit relays excitation to PSI core through a pair of chlorophyll molecules, thus revealing previously unseen paths for energy transfer between the antennas and the PSI core. | |||||||||||||||||||||||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 5zji.cif.gz | 1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb5zji.ent.gz | 913.5 KB | Display | PDB format |
PDBx/mmJSON format | 5zji.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zj/5zji ftp://data.pdbj.org/pub/pdb/validation_reports/zj/5zji | HTTPS FTP |
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-Related structure data
Related structure data | 6932MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
-Chlorophyll a-b binding protein, ... , 5 types, 5 molecules 1234Y
#1: Protein | Mass: 26335.127 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6SSN3 |
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#2: Protein | Mass: 29237.975 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6T892 |
#3: Protein | Mass: 29011.234 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6SUC4 |
#4: Protein | Mass: 27406.082 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6SZR1 |
#20: Protein | Mass: 24780.900 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6T1H1, UniProt: B6SZT9*PLUS |
-Photosystem I P700 chlorophyll a apoprotein ... , 2 types, 2 molecules AB
#5: Protein | Mass: 83193.156 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: P04966, photosystem I |
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#6: Protein | Mass: 82648.797 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays subsp. mays (maize) / References: UniProt: A0A059Q6U2, photosystem I |
-Protein , 3 types, 4 molecules COXZ
#7: Protein | Mass: 8909.345 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays subsp. mays (maize) / References: UniProt: A0A172FJJ4*PLUS |
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#17: Protein | Mass: 14381.669 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6SQZ7 |
#19: Protein | Mass: 24879.148 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: P12329 |
-Photosystem I reaction center subunit ... , 10 types, 10 molecules DEFGHIJKLN
#8: Protein | Mass: 21603.672 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B4FAW3 |
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#9: Protein | Mass: 14429.237 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: A0A1D6HY75 |
#10: Protein | Mass: 23833.559 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6SRI3 |
#11: Protein | Mass: 15329.372 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6U534 |
#12: Protein | Mass: 14948.259 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: O65101 |
#13: Protein/peptide | Mass: 4029.889 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: P30980 |
#14: Protein/peptide | Mass: 4747.620 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: P62596 |
#15: Protein | Mass: 13668.812 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6TR16 |
#16: Protein | Mass: 22347.809 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6STT2 |
#18: Protein | Mass: 15663.827 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Zea mays (maize) / References: UniProt: B6TXS5 |
-Sugars , 2 types, 4 molecules
#31: Sugar | #32: Sugar | |
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-Non-polymers , 11 types, 267 molecules
#21: Chemical | ChemComp-CHL / #22: Chemical | ChemComp-CLA / #23: Chemical | ChemComp-LUT / ( #24: Chemical | ChemComp-XAT / ( #25: Chemical | ChemComp-BCR / #26: Chemical | ChemComp-LHG / #27: Chemical | ChemComp-LMG / #28: Chemical | ChemComp-CL0 / | #29: Chemical | #30: Chemical | #33: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Supercomplex of plant PSI-LHCI-LHCII from Zea Mays / Type: COMPLEX / Entity ID: #1-#20 / Source: NATURAL |
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Source (natural) | Organism: Zea mays (maize) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy |
Image recording | Electron dose: 50 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.12_2829: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 635845 / Symmetry type: POINT | ||||||||||||||||||||||||
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