- PDB-5iv4: Crystal structure of the human soluble adenylyl cyclase in comple... -
+
Open data
ID or keywords:
Loading...
-
Basic information
Entry
Database: PDB / ID: 5iv4
Title
Crystal structure of the human soluble adenylyl cyclase in complex with the allosteric inhibitor LRE1
Components
Adenylate cyclase type 10
Keywords
LYASE / human soluble Adenylyl Cyclase complex LRE1 inhibitor
Function / homology
Function and homology information
negative regulation of cardiac muscle cell contraction / mitochondrial ATP transmembrane transport / epithelial cilium movement involved in extracellular fluid movement / bicarbonate binding / astrocyte end-foot / neuron projection retraction / : / central region of growth cone / positive regulation of glycogen catabolic process / glucose catabolic process ...negative regulation of cardiac muscle cell contraction / mitochondrial ATP transmembrane transport / epithelial cilium movement involved in extracellular fluid movement / bicarbonate binding / astrocyte end-foot / neuron projection retraction / : / central region of growth cone / positive regulation of glycogen catabolic process / glucose catabolic process / regulation of mitophagy / regulation of membrane repolarization / adenylate cyclase / basal part of cell / positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway / cAMP biosynthetic process / positive regulation of ossification / adenylate cyclase activity / positive regulation of protein targeting to mitochondrion / positive regulation of reactive oxygen species biosynthetic process / neuron projection extension / positive regulation of vascular associated smooth muscle cell apoptotic process / positive regulation of mitochondrial depolarization / positive regulation of ATP biosynthetic process / positive regulation of cardiac muscle hypertrophy / positive regulation of cardiac muscle cell apoptotic process / negative regulation of mitochondrial membrane potential / spermatid development / positive regulation of axon extension / Hedgehog 'off' state / negative regulation of reactive oxygen species biosynthetic process / neuron projection maintenance / positive regulation of peptidyl-threonine phosphorylation / cilium / manganese ion binding / ATPase binding / cytoskeleton / intracellular signal transduction / apical plasma membrane / neuronal cell body / dendrite / perinuclear region of cytoplasm / magnesium ion binding / mitochondrion / extracellular region / ATP binding / nucleus / cytosol / cytoplasm Similarity search - Function
Mass: 54269.664 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Cys255 modified by BME which is in the purification buffer Source: (gene. exp.) Homo sapiens (human) / Gene: ADCY10, SAC / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q96PN6, adenylate cyclase
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.918007 Å / Relative weight: 1
Reflection
Resolution: 1.79→44.42 Å / Num. obs: 599809 / % possible obs: 99.9 % / Redundancy: 11.4 % / Rmerge(I) obs: 0.115 / Net I/σ(I): 16.3
Reflection shell
Resolution: 1.79→1.9 Å / Redundancy: 11.2 % / Rmerge(I) obs: 1.192 / Mean I/σ(I) obs: 2.07 / % possible all: 99.5
-
Processing
Software
Name
Version
Classification
REFMAC
5.8.0131
refinement
XDS
datareduction
Coot
modelbuilding
MOLREP
phasing
XSCALE
datascaling
Refinement
Resolution: 1.79→44.42 Å / Cor.coef. Fo:Fc: 0.971 / Cor.coef. Fo:Fc free: 0.953 / SU B: 2.647 / SU ML: 0.08 / Cross valid method: THROUGHOUT / ESU R: 0.103 / ESU R Free: 0.109 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.20626
2621
5 %
RANDOM
Rwork
0.15972
-
-
-
obs
0.162
49787
99.92 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK