Mass: 18.015 Da / Num. of mol.: 153 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 2.61 Å3/Da / Density % sol: 52.84 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6.7 Details: Crystals are formed by mixing in a 1:1 ratio endonuclease (5 mg/ml) with crystallization buffer containing 200 mM MES, 27% PEG8000, 200 mM ammonium sulfate, 1 mM manganese chloride, 10 mM ...Details: Crystals are formed by mixing in a 1:1 ratio endonuclease (5 mg/ml) with crystallization buffer containing 200 mM MES, 27% PEG8000, 200 mM ammonium sulfate, 1 mM manganese chloride, 10 mM magnesium acetate, 10 mM taurine, and 50 mM sodium fluoride. Crystals form within a few hours and grow to maximum size in one to two weeks
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Data collection
Diffraction
Mean temperature: 100 K Ambient temp details: . X-ray diffraction data collection was performed at the Cornell High Energy Synchrotron Source (CHESS) F1 beamline. The diffraction data were indexed, processed, scaled and ...Ambient temp details: . X-ray diffraction data collection was performed at the Cornell High Energy Synchrotron Source (CHESS) F1 beamline. The diffraction data were indexed, processed, scaled and merged using HKL2000.29 The structure was solved and refined using the software PHENIX.30
Diffraction source
Source: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.917 Å
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