ジャーナル: Acta Crystallogr.,Sect.D / 年: 2015 タイトル: Biochemical and structural investigation of two paralogous glycoside hydrolases from Zobellia galactanivorans: novel insights into the evolution, dimerization plasticity and catalytic ...タイトル: Biochemical and structural investigation of two paralogous glycoside hydrolases from Zobellia galactanivorans: novel insights into the evolution, dimerization plasticity and catalytic mechanism of the GH117 family. 著者: Ficko-Blean, E. / Duffieux, D. / Rebuffet, E. / Larocque, R. / Groisillier, A. / Michel, G. / Czjzek, M.
温度: 291 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: The optimized condition for Zg3615 was: 0.1 M sodium acetate pH 5.0, 25% PEG 3350, in a 1:1 ratio of protein (at 10 mg/mL) to mother liquor.
解像度: 1.7→30 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.944 / SU B: 1.947 / SU ML: 0.066 / 交差検証法: THROUGHOUT / ESU R: 0.106 / ESU R Free: 0.105 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
反射数
%反射
Selection details
Rfree
0.21888
4838
5 %
RANDOM
Rwork
0.18485
-
-
-
obs
0.18655
91803
99.96 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK