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Open data
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Basic information
Entry | Database: PDB / ID: 4f8z | ||||||
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Title | Carboxypeptidase T with Boc-Leu | ||||||
![]() | Carboxypeptidase T | ||||||
![]() | HYDROLASE | ||||||
Function / homology | ![]() carboxypeptidase T / metallocarboxypeptidase activity / proteolysis / extracellular space / zinc ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Timofeev, V.I. / Kuznetsov, S.A. / Akparov, V.K. / Kuranova, I.P. | ||||||
![]() | ![]() Title: Three-dimensional structure of carboxypeptidase T from Thermoactinomyces vulgaris in complex with N-BOC-L-leucine. Authors: Timofeev, V.I. / Kuznetsov, S.A. / Akparov, V.K.h. / Chestukhina, G.G. / Kuranova, I.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 168 KB | Display | ![]() |
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PDB format | ![]() | 141.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 36641.277 Da / Num. of mol.: 1 / Fragment: UNP residues 99-421 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 6 types, 450 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/BL2.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/BL2.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-ZN / | ||||||
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#3: Chemical | ChemComp-BL2 / | ||||||
#4: Chemical | #5: Chemical | ChemComp-CA / #6: Chemical | ChemComp-GOL / #7: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | pH: 6 Details: COUNTER-DIFFUSION. protein solution: 250 mM NaCl, 1 mM CaCl2, 10 mM Mes/NaOH, pH 6.0. precipitant solution: 1.4 M (NH4)2SO4, 5% MPD, 50 mM, Mes/NaOH, pH 6.0, 2.4 M Boc-L-Leu |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX225HE / Detector: CCD / Date: Dec 13, 2010 / Details: mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8 Å / Relative weight: 1 |
Reflection | Resolution: 1.12→30 Å / Num. all: 286940 / Num. obs: 286940 / % possible obs: 100 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 17.185 Å2
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Refinement step | Cycle: LAST / Resolution: 1.38→14 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.38→1.415 Å / Total num. of bins used: 20
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