+Open data
-Basic information
Entry | Database: PDB / ID: 4czg | ||||||
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Title | C. crescentus MreB, single filament, ADP, A22 inhibitor | ||||||
Components | ROD SHAPE-DETERMINING PROTEIN MREB | ||||||
Keywords | STRUCTURAL PROTEIN / BACTERIAL ACTIN / BACTERIAL CYTOSKELETON | ||||||
Function / homology | Function and homology information | ||||||
Biological species | CAULOBACTER VIBRIOIDES (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||
Authors | Lowe, J. / van den Ent, F. | ||||||
Citation | Journal: Elife / Year: 2014 Title: Bacterial Actin Mreb Forms Antiparallel Double Filaments. Authors: Van Den Ent, F. / Izore, T. / Bharat, T.A. / Johnson, C.M. / Lowe, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4czg.cif.gz | 201.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4czg.ent.gz | 160.3 KB | Display | PDB format |
PDBx/mmJSON format | 4czg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 4czg_validation.pdf.gz | 760.9 KB | Display | wwPDB validaton report |
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Full document | 4czg_full_validation.pdf.gz | 764.1 KB | Display | |
Data in XML | 4czg_validation.xml.gz | 18.7 KB | Display | |
Data in CIF | 4czg_validation.cif.gz | 29 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cz/4czg ftp://data.pdbj.org/pub/pdb/validation_reports/cz/4czg | HTTPS FTP |
-Related structure data
Related structure data | 4czeC 4czfC 4czhC 4cziC 4czjC 4czkC 4czlC 4czmC 1jceS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36865.496 Da / Num. of mol.: 1 / Fragment: RESIDUES 9-347 / Mutation: YES Source method: isolated from a genetically manipulated source Details: M-I9-CCMREB(F102S, V103G)-A347-GSHHHHHH / Source: (gene. exp.) CAULOBACTER VIBRIOIDES (bacteria) / Plasmid: PHIS17 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): C41 / References: UniProt: B8H609, UniProt: A0A0H3C7V4*PLUS |
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#2: Chemical | ChemComp-QH3 / |
#3: Chemical | ChemComp-ADP / |
#4: Chemical | ChemComp-MG / |
#5: Water | ChemComp-HOH / |
Nonpolymer details | A22, S-(3,4-DICHLOROBE |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.14 % / Description: NONE |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU FR-E / Wavelength: 1.5418 |
Detector | Type: MARESEARCH / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→50 Å / Num. obs: 52173 / % possible obs: 87.8 % / Observed criterion σ(I): 0 / Redundancy: 6.8 % / Biso Wilson estimate: 20.8 Å2 / Rmerge(I) obs: 0.05 / Net I/σ(I): 19.8 |
Reflection shell | Resolution: 1.5→1.58 Å / Redundancy: 5.4 % / Rmerge(I) obs: 0.69 / Mean I/σ(I) obs: 1.1 / % possible all: 77 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1JCE Resolution: 1.5→20.397 Å / SU ML: 0.45 / σ(F): 1.34 / Phase error: 23.25 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.77 Å / VDW probe radii: 0.9 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 60.873 Å2 / ksol: 0.459 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.5→20.397 Å
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Refine LS restraints |
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LS refinement shell |
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