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- EMDB-4475: Structure of tmRNA SmpB bound in A site of T. thermophilus 70S ri... -

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Basic information

Entry
Database: EMDB / ID: EMD-4475
TitleStructure of tmRNA SmpB bound in A site of T. thermophilus 70S ribosome
Map data
Sample
  • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • Protein or peptide: x 1 types
      • RNA: x 1 types
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • Protein or peptide: x 1 types
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • Protein or peptide: x 1 types
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • RNA: x 3 types
      • Protein or peptide: x 50 types
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • RNA: x 2 types
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • RNA: x 1 types
  • Ligand: x 2 types
Function / homology
Function and homology information


trans-translation / large ribosomal subunit / regulation of translation / small ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome ...trans-translation / large ribosomal subunit / regulation of translation / small ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / zinc ion binding / metal ion binding / cytoplasm
Similarity search - Function
SsrA-binding protein / SsrA-binding protein, conserved site / Small protein B / SmpB protein / SsrA-binding protein. / Ribosomal protein L1, bacterial-type / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / Ribosomal protein L25, long-form ...SsrA-binding protein / SsrA-binding protein, conserved site / Small protein B / SmpB protein / SsrA-binding protein. / Ribosomal protein L1, bacterial-type / 30S ribosomal protein Thx / 30S ribosomal protein Thx / 30S ribosomal protein / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S14, type Z / Ribosomal protein L1, conserved site / Ribosomal protein L1 / Ribosomal protein L1 signature. / Ribosomal protein L1, 3-layer alpha/beta-sandwich / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein S14/S29 / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16
Similarity search - Domain/homology
Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL19 ...Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL25 / Small ribosomal subunit protein bTHX / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL31 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein uL1 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein bL36 / SsrA-binding protein / Large ribosomal subunit protein bL17
Similarity search - Component
Biological speciesThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria) / Escherichia coli (E. coli) / Thermus thermophilus HB27 (bacteria) / Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.7 Å
AuthorsRae CD
Funding support United Kingdom, 2 items
OrganizationGrant numberCountry
Medical Research Council (United Kingdom)MC_U105184332 United Kingdom
Wellcome TrustWT096570 United Kingdom
CitationJournal: Science / Year: 2019
Title: How a circularized tmRNA moves through the ribosome.
Authors: Christopher D Rae / Yuliya Gordiyenko / V Ramakrishnan /
Abstract: During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo- ...During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA.
History
DepositionDec 17, 2018-
Header (metadata) releaseFeb 27, 2019-
Map releaseFeb 27, 2019-
UpdateNov 6, 2019-
Current statusNov 6, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.05
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6q95
  • Surface level: 0.05
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4475.png

Downloads & links

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Map

FileDownload / File: emd_4475.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.34 Å
Density
Contour LevelBy AUTHOR: 0.05 / Movie #1: 0.05
Minimum - Maximum-0.20200269 - 0.4199737
Average (Standard dev.)0.0009820506 (±0.01193797)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 482.40002 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.341.341.34
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z482.400482.400482.400
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ364364364
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-0.2020.4200.001

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Supplemental data

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Sample components

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Entire : 70S ribosome bound by tmRNA-SmpB in the A site

EntireName: 70S ribosome bound by tmRNA-SmpB in the A site
Components
  • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • Protein or peptide: SsrA-binding protein
      • RNA: transfer-messenger RNA (tmRNA)
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • Protein or peptide: Nascent peptide MET-ALA
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • Protein or peptide: 50S ribosomal protein L36
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • RNA: 16S RIBOSOMAL RNA
      • Protein or peptide: 30S ribosomal protein S2
      • Protein or peptide: 30S ribosomal protein S3
      • Protein or peptide: 30S ribosomal protein S4
      • Protein or peptide: 30S ribosomal protein S5
      • Protein or peptide: 30S ribosomal protein S6
      • Protein or peptide: 30S ribosomal protein S7
      • Protein or peptide: 30S ribosomal protein S8
      • Protein or peptide: 30S ribosomal protein S9
      • Protein or peptide: 30S ribosomal protein S10
      • Protein or peptide: 30S ribosomal protein S11
      • Protein or peptide: 30S ribosomal protein S12
      • Protein or peptide: 30S ribosomal protein S13
      • Protein or peptide: 30S ribosomal protein S14 type ZRibosome
      • Protein or peptide: 30S ribosomal protein S15
      • Protein or peptide: 30S ribosomal protein S16
      • Protein or peptide: 30S ribosomal protein S17
      • Protein or peptide: 30S ribosomal protein S18
      • Protein or peptide: 30S ribosomal protein S19
      • Protein or peptide: 30S ribosomal protein S20
      • Protein or peptide: 30S ribosomal protein ThxRibosome
      • Protein or peptide: 50S ribosomal protein L27
      • Protein or peptide: 50S ribosomal protein L28
      • Protein or peptide: 50S ribosomal protein L29
      • Protein or peptide: 50S ribosomal protein L30
      • Protein or peptide: 50S ribosomal protein L31
      • Protein or peptide: 50S ribosomal protein L32
      • Protein or peptide: 50S ribosomal protein L33
      • Protein or peptide: 50S ribosomal protein L34
      • Protein or peptide: 50S ribosomal protein L35
      • RNA: 23S RIBOSOMAL RNA
      • RNA: 5S RIBOSOMAL RNA
      • Protein or peptide: 50S ribosomal protein L1
      • Protein or peptide: 50S ribosomal protein L2
      • Protein or peptide: 50S ribosomal protein L3
      • Protein or peptide: 50S ribosomal protein L4
      • Protein or peptide: 50S ribosomal protein L5
      • Protein or peptide: 50S ribosomal protein L6
      • Protein or peptide: 50S ribosomal protein L9
      • Protein or peptide: 50S ribosomal protein L13
      • Protein or peptide: 50S ribosomal protein L14
      • Protein or peptide: 50S ribosomal protein L15
      • Protein or peptide: 50S ribosomal protein L16
      • Protein or peptide: 50S ribosomal protein L17
      • Protein or peptide: 50S ribosomal protein L18
      • Protein or peptide: 50S ribosomal protein L19
      • Protein or peptide: 50S ribosomal protein L20
      • Protein or peptide: 50S ribosomal protein L21
      • Protein or peptide: 50S ribosomal protein L22
      • Protein or peptide: 50S ribosomal protein L23
      • Protein or peptide: 50S ribosomal protein L24
      • Protein or peptide: 50S ribosomal protein L25
      • Protein or peptide: 50S RIBOSOMAL PROTEIN L10 AND L11Ribosome
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • RNA: tRNA-fMet
      • RNA: tRNA-Phe
    • Complex: 70S ribosome bound by tmRNA-SmpB in the A site
      • RNA: mRNAMessenger RNA
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: 70S ribosome bound by tmRNA-SmpB in the A site

SupramoleculeName: 70S ribosome bound by tmRNA-SmpB in the A site / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#60

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Supramolecule #2: 70S ribosome bound by tmRNA-SmpB in the A site

SupramoleculeName: 70S ribosome bound by tmRNA-SmpB in the A site / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #56, #60
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #3: 70S ribosome bound by tmRNA-SmpB in the A site

SupramoleculeName: 70S ribosome bound by tmRNA-SmpB in the A site / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #57
Source (natural)Organism: Escherichia coli (E. coli)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #4: 70S ribosome bound by tmRNA-SmpB in the A site

SupramoleculeName: 70S ribosome bound by tmRNA-SmpB in the A site / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #58
Source (natural)Organism: Thermus thermophilus HB27 (bacteria)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #5: 70S ribosome bound by tmRNA-SmpB in the A site

SupramoleculeName: 70S ribosome bound by tmRNA-SmpB in the A site / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #1-#21, #25-#55, #59
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)

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Supramolecule #6: 70S ribosome bound by tmRNA-SmpB in the A site

SupramoleculeName: 70S ribosome bound by tmRNA-SmpB in the A site / type: complex / ID: 6 / Parent: 1 / Macromolecule list: #22-#23
Source (natural)Organism: Escherichia coli (E. coli)

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Supramolecule #7: 70S ribosome bound by tmRNA-SmpB in the A site

SupramoleculeName: 70S ribosome bound by tmRNA-SmpB in the A site / type: complex / ID: 7 / Parent: 1 / Macromolecule list: #24
Source (natural)Organism: Escherichia coli (E. coli)
Recombinant expressionOrganism: synthetic construct (others)

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Macromolecule #1: 16S RIBOSOMAL RNA

MacromoleculeName: 16S RIBOSOMAL RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 493.958281 KDa
SequenceString: UUUGUUGGAG AGUUUGAUCC UGGCUCAGGG UGAACGCUGG CGGCGUGCCU AAGACAUGCA AGUCGUGCGG GCCGCGGGGU UUUACUCCG UGGUCAGCGG CGGACGGGUG AGUAACGCGU GGGUGACCUA CCCGGAAGAG GGGGACAACC CGGGGAAACU C GGGCUAAU ...String:
UUUGUUGGAG AGUUUGAUCC UGGCUCAGGG UGAACGCUGG CGGCGUGCCU AAGACAUGCA AGUCGUGCGG GCCGCGGGGU UUUACUCCG UGGUCAGCGG CGGACGGGUG AGUAACGCGU GGGUGACCUA CCCGGAAGAG GGGGACAACC CGGGGAAACU C GGGCUAAU CCCCCAUGUG GACCCGCCCC UUGGGGUGUG UCCAAAGGGC UUUGCCCGCU UCCGGAUGGG CCCGCGUCCC AU CAGCUAG UUGGUGGGGU AAUGGCCCAC CAAGGCGACG ACGGGUAGCC GGUCUGAGAG GAUGGCCGGC CACAGGGGCA CUG AGACAC GGGCCCCACU CCUACGGGAG GCAGCAGUUA GGAAUCUUCC GCAAUGGGCG CAAGCCUGAC GGAGCGACGC CGCU UGGAG GAAGAAGCCC UUCGGGGUGU AAACUCCUGA ACCCGGGACG AAACCCCCGA CGAGGGGACU GACGGUACCG GGGUA AUAG CGCCGGCCAA CUCCGUGCCA GCAGCCGCGG UAAUACGGAG GGCGCGAGCG UUACCCGGAU UCACUGGGCG UAAAGG GCG UGUAGGCGGC CUGGGGCGUC CCAUGUGAAA GACCACGGCU CAACCGUGGG GGAGCGUGGG AUACGCUCAG GCUAGAC GG UGGGAGAGGG UGGUGGAAUU CCCGGAGUAG CGGUGAAAUG CGCAGAUACC GGGAGGAACG CCGAUGGCGA AGGCAGCC A CCUGGUCCAC CCGUGACGCU GAGGCGCGAA AGCGUGGGGA GCAAACCGGA UUAGAUACCC GGGUAGUCCA CGCCCUAAA CGAUGCGCGC UAGGUCUCUG GGUCUCCUGG GGGCCGAAGC UAACGCGUUA AGCGCGCCGC CUGGGGAGUA CGGCCGCAAG GCUGAAACU CAAAGGAAUU GACGGGGGCC CGCACAAGCG GUGGAGCAUG UGGUUUAAUU CGAAGCAACG CGAAGAACCU U ACCAGGCC UUGACAUGCU AGGGAACCCG GGUGAAAGCC UGGGGUGCCC CGCGAGGGGA GCCCUAGCAC AGGUGCUGCA UG GCCGUCG UCAGCUCGUG CCGUGAGGUG UUGGGUUAAG UCCCGCAACG AGCGCAACCC CCGCCGUUAG UUGCCAGCGG UUC GGCCGG GCACUCUAAC GGGACUGCCC GCGAAAGCGG GAGGAAGGAG GGGACGACGU CUGGUCAGCA UGGCCCUUAC GGCC UGGGC GACACACGUG CUACAAUGCC CACUACAAAG CGAUGCCACC CGGCAACGGG GAGCUAAUCG CAAAAAGGUG GGCCC AGUU CGGAUUGGGG UCUGCAACCC GACCCCAUGA AGCCGGAAUC GCUAGUAAUC GCGGAUCAGC CAUGCCGCGG UGAAUA CGU UCCCGGGCCU UGUACACACC GCCCGUCACG CCAUGGGAGC GGGCUCUACC CGAAGUCGCC GGGAGCCUAC GGGCAGG CG CCGAGGGUAG GGCCCGUGAC UGGGGCGAAG UCGUAACAAG GUAGCUGUAC CGGAAGGUGC GGCUGGAUCA CCUCCUUU C U

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Macromolecule #22: tRNA-fMet

MacromoleculeName: tRNA-fMet / type: rna / ID: 22 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.816811 KDa
SequenceString:
CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UCGG(5MU)UCAAA UCCGGCCCCC GCAACC A

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Macromolecule #23: tRNA-Phe

MacromoleculeName: tRNA-Phe / type: rna / ID: 23 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 24.400418 KDa
SequenceString:
GCCCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUUUUAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA

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Macromolecule #24: mRNA

MacromoleculeName: mRNA / type: rna / ID: 24 / Number of copies: 1
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 1.853133 KDa
SequenceString:
UUCAUG

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Macromolecule #34: 23S RIBOSOMAL RNA

MacromoleculeName: 23S RIBOSOMAL RNA / type: rna / ID: 34 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 947.935438 KDa
SequenceString: GGUCAAGAUG GUAAGGGCCC ACGGUGGAUG CCUCGGCACC CGAGCCGAUG AAGGACGUGG CUACCUGCGA UAAGCCAGGG GGAGCCGGU AGCGGGCGUG GAUCCCUGGA UGUCCGAAUG GGGGAACCCG GCCGGCGGGA ACGCCGGUCA CCGCGCUUUU G CGCGGGGG ...String:
GGUCAAGAUG GUAAGGGCCC ACGGUGGAUG CCUCGGCACC CGAGCCGAUG AAGGACGUGG CUACCUGCGA UAAGCCAGGG GGAGCCGGU AGCGGGCGUG GAUCCCUGGA UGUCCGAAUG GGGGAACCCG GCCGGCGGGA ACGCCGGUCA CCGCGCUUUU G CGCGGGGG GAACCUGGGG AACUGAAACA UCUCAGUACC CAGAGGAGAG GAAAGAGAAA UCGACUCCCU GAGUAGCGGC GA GCGAAAG GGGACCAGCC UAAACCGUCC GGCUUGUCCG GGCGGGGUCG UGGGGCCCUC GGACACCGAA UCCCCAGCCU AGC CGAAGC UGUUGGGAAG CAGCGCCAGA GAGGGUGAAA GCCCCGUAGG CGAAAGGUGG GGGGAUAGGU GAGGGUACCC GAGU ACCCC GUGGUUCGUG GAGCCAUGGG GGAAUCUGGG CGGACCACCG CCUAAGGCUA AGUACUCCGG GUGACCGAUA GCGCA CCAG UACCGUGAGG GAAAGGUGAA AAGAACCCCG GGAGGGGAGU GAAAUAGAGC CUGAAACCGU GGGCUUACAA GCAGUC ACG GCCCCGCAAG GGGUUGUGGC GUGCCUAUUG AAGCAUGAGC CGGCGACUCA CGGUCGUGGG CGAGCUUAAG CCGUUGA GG CGGAGGCGUA GGGAAACCGA GUCCGAACAG GGCGCAAGCG GGCCGCACGC GGCCCGCAAA GUCCGCGGCC GUGGACCC G AAACCGGGCG AGCUAGCCCU GGCCAGGGUG AAGCUGGGGU GAGACCCAGU GGAGGCCCGA ACCGGUGGGG GAUGCAAAC CCCUCGGAUG AGCUGGGGCU AGGAGUGAAA AGCUAACCGA GCCCGGAGAU AGCUGGUUCU CCCCGAAAUG ACUUUAGGGU CAGCCUCAG GCGCUGACUG GGGCCUGUAG AGCACUGAUA GGGCUAGGGG GCCCACCAGC CUACCAAACC CUGUCAAACU C CGAAGGGU CCCAGGUGGA GCCUGGGAGU GAGGGCGCGA GCGAUAACGU CCGCGUCCGA GCGCGGGAAC AACCGAGACC GC CAGCUAA GGCCCCCAAG UCUGGGCUAA GUGGUAAAGG AUGUGGCGCC GCGAAGACAG CCAGGAGGUU GGCUUAGAAG CAG CCAUCC UUUAAAGAGU GCGUAAUAGC UCACUGGUCG AGUGGCGCCG CGCCGAAAAU GAUCGGGGCU UAAGCCCAGC GCCG AAGCU GCGGGUCUGG GGGAUGACCC CAGGCGGUAG GGGAGCGUUC CCGAUGCCGA UGAAGGCCGA CCCGCGAGGG CGGCU GGAG GUAAGGGAAG UGCGAAUGCC GGCAUGAGUA ACGAUAAAGA GGGUGAGAAU CCCUCUCGCC GUAAGCCCAA GGGUUC CUA CGCAAUGGUC GUCAGCGUAG GGUUAGGCGG GACCUAAGGU GAAGCCGAAA GGCGUAGCCG AAGGGCAGCC GGUUAAU AU UCCGGCCCUU CCCGCAGGUG CGAUGGGGGG ACGCUCUAGG CUAGGGGGAC CGGAGCCAUG GACGAGCCCG GCCAGAAG C GCAGGGUGGG AGGUAGGCAA AUCCGCCUCC CAACAAGCUC UGCGUGGUGG GGAAGCCCGU ACGGGUGACA ACCCCCCGA AGCCAGGGAG CCAAGAAAAG CCUCUAAGCA CAACCUGCGG GAACCCGUAC CGCAAACCGA CACAGGUGGG CGGGUGCAAG AGCACUCAG GCGCGCGGGA GAACCCUCGC CAAGGAACUC UGCAAGUUGG CCCCGUAACU UCGGGAGAAG GGGUGCUCCC U GGGGUGAU GAGCCCCGGG GAGCCGCAGU GAACAGGCUC UGGCGACUGU UUACCAAAAA CACAGCUCUC UGCGAACUCG UA AGAGGAG GUAUAGGGAG CGACGCUUGC CCGGUGCCGG AAGGUCAAGG GGAGGGGUGC AAGCCCCGAA CCGAAGCCCC GGU GAACGG CGGCCGUAAC UAUAACGGUC CUAAGGUAGC GAAAUUCCUU GUCGGGUAAG UUCCGACCUG CACGAAAAGC GUAA CGACC GGAGCGCUGU CUCGGCGAGG GACCCGGUGA AAUUGAACUG GCCGUGAAGA UGCGGCCUAC CCGUGGCAGG ACGAA AAGA CCCCGUGGAG CUUUACUGCA GCCUGGUGUU GGCUCUUGGU CGCGCCUGCG UAGGAUAGGU GGGAGCCUGU GAACCC CCG CCUCCGGGUG GGGGGGAGGC GCCGGUGAAA UACCACCCUG GCGCGGCUGG GGGCCUAACC CUCGGAUGGG GGGACAG CG CUUGGCGGGC AGUUUGACUG GGGCGGUCGC CUCCUAAAAG GUAACGGAGG CGCCCAAAGG UCCCCUCAGG CGGGACGG A AAUCCGCCGG AGAGCGCAAG GGUAGAAGGG GGCCUGACUG CGAGGCCUGC AAGCCGAGCA GGGGCGAAAG CCGGGCCUA GUGAACCGGU GGUCCCGUGU GGAAGGGCCA UCGAUCAACG GAUAAAAGUU ACCCCGGGGA UAACAGGCUG AUCUCCCCCG AGCGUCCAC AGCGGCGGGG AGGUUUGGCA CCUCGAUGUC GGCUCGUCGC AUCCUGGGGC UGAAGAAGGU CCCAAGGGUU G GGCUGUUC GCCCAUUAAA GCGGCACGCG AGCUGGGUUC AGAACGUCGU GAGACAGUUC GGUCUCUAUC CGCCACGGGC GC AGGAGGC UUGAGGGGGG CUCUUCCUAG UACGAGAGGA CCGGAAGGGA CGCACCUCUG GUUUCCCAGC UGUCCCUCCA GGG GCAUAA GCUGGGUAGC CAUGUGCGGA AGGGAUAACC GCUGAAAGCA UCUAAGCGGG AAGCCCGCCC CAAGAUGAGG CCUC CCACG GCGUCAAGCC GGUAAGGACC CGGGAAGACC ACCCGGUGGA UGGGCCGGGG GUGUAAGCGC CGCGAGGCGU UGAGC CGAC CGGUCCCAAU CGUCCGAGGU CUUGACCCCU C

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Macromolecule #35: 5S RIBOSOMAL RNA

MacromoleculeName: 5S RIBOSOMAL RNA / type: rna / ID: 35 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 38.553 KDa
SequenceString:
UCCCCCGUGC CCAUAGCGGC GUGGAACCAC CCGUUCCCAU UCCGAACACG GAAGUGAAAC GCGCCAGCGC CGAUGGUACU GGGCGGGCG ACCGCCUGGG AGAGUAGGUC GGUGCGGGGG

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Macromolecule #60: transfer-messenger RNA (tmRNA)

MacromoleculeName: transfer-messenger RNA (tmRNA) / type: rna / ID: 60 / Number of copies: 1
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 112.818945 KDa
SequenceString: GGGGGUGAAA CGGUCUCGAC GGGGGUCGCC GAGGGCGUGG CUGCGCGCCG AGGUGCGGGU GGCCUCGUAA AAACCCGCAA CGGCAUAAC UGCCAACACC AACUACGCUC UCGCGGCUUA AUGACCGCGA CCUCGCCCGG UAGCCCUGCC GGGGGCUCAC C GGAAGCGG ...String:
GGGGGUGAAA CGGUCUCGAC GGGGGUCGCC GAGGGCGUGG CUGCGCGCCG AGGUGCGGGU GGCCUCGUAA AAACCCGCAA CGGCAUAAC UGCCAACACC AACUACGCUC UCGCGGCUUA AUGACCGCGA CCUCGCCCGG UAGCCCUGCC GGGGGCUCAC C GGAAGCGG GGACACAAAC CCGGCUAGCC CGGGGCCACG CCCUCUAACC CCGGGCGAAG CUUGAAGGGG GCUCGCUCCU GG CCGCCCG UCCGCGGGCC AAGCCAGGAG GACACGCGAA ACGCGGACUA CGCGCGUAGA GGCCCGCCGU AGGGACCUUC GGA CGGGGG UUCGACUCCC CCCACCUCCA CCA

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Macromolecule #2: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 27.116385 KDa
SequenceString: VKELLEAGVH FGHERKRWNP KFARYIYAER NGIHIIDLQK TMEELERTFR FIEDLAMRGG TILFVGTKKQ AQDIVRMEAE RAGMPYVNQ RWLGGMLTNF KTISQRVHRL EELEALFASP EIEERPKKEQ VRLKHELERL QKYLSGFRLL KRLPDAIFVV D PTKEAIAV ...String:
VKELLEAGVH FGHERKRWNP KFARYIYAER NGIHIIDLQK TMEELERTFR FIEDLAMRGG TILFVGTKKQ AQDIVRMEAE RAGMPYVNQ RWLGGMLTNF KTISQRVHRL EELEALFASP EIEERPKKEQ VRLKHELERL QKYLSGFRLL KRLPDAIFVV D PTKEAIAV REARKLFIPV IALADTDSDP DLVDYIIPGN DDAIRSIQLI LSRAVDLIIQ ARGGVVEPSP SYALVQE

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Macromolecule #3: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 22.975588 KDa
SequenceString: GNKIHPIGFR LGITRDWESR WYAGKKQYRH LLLEDQRIRG LLEKELYSAG LARVDIERAA DNVAVTVHVA KPGVVIGRGG ERIRVLREE LAKLTGKNVA LNVQEVQNPN LSAPLVAQRV AEQIERRFAV RRAIKQAVQR VMESGAKGAK VIVSGRIGGA E QARTEWAA ...String:
GNKIHPIGFR LGITRDWESR WYAGKKQYRH LLLEDQRIRG LLEKELYSAG LARVDIERAA DNVAVTVHVA KPGVVIGRGG ERIRVLREE LAKLTGKNVA LNVQEVQNPN LSAPLVAQRV AEQIERRFAV RRAIKQAVQR VMESGAKGAK VIVSGRIGGA E QARTEWAA QGRVPLHTLR ANIDYGFALA RTTYGVLGVK AYIFLGEVI

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Macromolecule #4: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 24.242254 KDa
SequenceString: GRYIGPVCRL CRREGVKLYL KGERCYSPKC AMERRPYPPG QHGQKRARRP SDYAVRLREK QKLRRIYGIS ERQFRNLFEE ASKKKGVTG SVFLGLLESR LDNVVYRLGF AVSRRQARQL VRHGHITVNG RRVDLPSYRV RPGDEIAVAE KSRNLELIRQ N LEAMKGRK ...String:
GRYIGPVCRL CRREGVKLYL KGERCYSPKC AMERRPYPPG QHGQKRARRP SDYAVRLREK QKLRRIYGIS ERQFRNLFEE ASKKKGVTG SVFLGLLESR LDNVVYRLGF AVSRRQARQL VRHGHITVNG RRVDLPSYRV RPGDEIAVAE KSRNLELIRQ N LEAMKGRK VGPWLSLDVE GMKGKFLRLP DREDLALPVN EQLVIEFYSR

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Macromolecule #5: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 16.460193 KDa
SequenceString:
DFEEKMILIR RTARMQAGGR RFRFGALVVV GDRQGRVGLG FGKAPEVPLA VQKAGYYARR NMVEVPLQNG TIPHEIEVEF GASKIVLKP AAPGTGVIAG AVPRAILELA GVTDILTKEL GSRNPINIAY ATMEALRQLR TKADVERLRK GE

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Macromolecule #6: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.988753 KDa
SequenceString:
MRRYEVNIVL NPNLDQSQLA LEKEIIQRAL ENYGARVEKV EELGLRRLAY PIAKDPQGYF LWYQVEMPED RVNDLARELR IRDNVRRVM VVKSQEPFLA NA

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Macromolecule #7: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 17.919775 KDa
SequenceString:
ARRRRAEVRQ LQPDLVYGDV LVTAFINKIM RDGKKNLAAR IFYDACKIIQ EKTGQEPLKV FKQAVENVKP RMEVRSRRVG GANYQVPME VSPRRQQSLA LRWLVQAANQ RPERRAAVRI AHELMDAAEG KGGAVKKKED VERMAEANRA YAHYRW

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Macromolecule #8: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 15.86857 KDa
SequenceString:
MLTDPIADML TRIRNATRVY KESTDVPASR FKEEILRILA REGFIKGYER VDVDGKPYLR VYLKYGPRRQ GPDPRPEQVI HHIRRISKP GRRVYVGVKE IPRVRRGLGI AILSTSKGVL TDREARKLGV GGELICEVW

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Macromolecule #9: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 14.298466 KDa
SequenceString:
EQYYGTGRRK EAVARVFLRP GNGKVTVNGQ DFNEYFQGLV RAVAALEPLR AVDALGRFDA YITVRGGGKS GQIDAIKLGI ARALVQYNP DYRAKLKPLG FLTRDARVVE RKKYGKHKAR RAPQYSKR

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Macromolecule #10: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.398308 KDa
SequenceString:
KIRIKLRGFD HKTLDASAQK IVEAARRSGA QVSGPIPLPT RVRRFTVIRG PFKHKDSREH FELRTHNRLV DIINPNRKTI EQLMTLDLP TGVEIEIKTV

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Macromolecule #11: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 12.606369 KDa
SequenceString:
KRQVASGRAY IHASYNNTIV TITDPDGNPI TWSSGGVIGY KGSRKGTPYA AQLAALDAAK KAMAYGMQSV DVIVRGTGAG REQAIRALQ ASGLQVKSIV DDTPVPHNGC RPKKKFRKAS

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Macromolecule #12: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 13.875388 KDa
SequenceString:
PTINQLVRKG REKVRKKSKV PALKGAPFRR GVCTVVRTVT PKKPNSALRK VAKVRLTSGY EVTAYIPGEG HNLQEHSVVL IRGGRVKDL PGVRYHIVRG VYDAAGVKDR KKSRSKYGTK KPKEAA

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Macromolecule #13: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 13.62392 KDa
SequenceString:
ARIAGVEIPR NKRVDVALTY IYGIGKARAK EALEKTGINP ATRVKDLTEA EVVRLREYVE NTWKLEGELR AEVAANIKRL MDIGCYRGL RHRRGLPVRG QRTRTNARTR KGPRKTVAGK K

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Macromolecule #14: 30S ribosomal protein S14 type Z

MacromoleculeName: 30S ribosomal protein S14 type Z / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 7.027529 KDa
SequenceString:
ARKALIEKAK RTPKFKVRAY TRCVRCGRAR SVYRFFGLCR ICLRELAHKG QLPGVRKASW

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Macromolecule #15: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 10.447213 KDa
SequenceString:
PITKEEKQKV IQEFARFPGD TGSTEVQVAL LTLRINRLSE HLKVHKKDHH SHRGLLMMVG QRRRLLRYLQ REDPERYRAL IEKLGIRG

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Macromolecule #16: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 9.995546 KDa
SequenceString:
MVKIRLARFG SKHNPHYRIV VTDARRKRDG KYIEKIGYYD PRKTTPDWLK VDVERARYWL SVGAQPTDTA RRLLRQAGVF RQEA

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Macromolecule #17: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.880098 KDa
SequenceString:
PKKVLTGVVV SDKMQKTVTV LVERQFPHPL YGKVIKRSKK YLAHDPEEKY KLGDVVEIIE SRPISKRKRF RVLRLVESGR MDLVEKYLI RRQNYESLSK R

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Macromolecule #18: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 8.155812 KDa
SequenceString:
KAKVKATLGE FDLRDYRNVE VLKRFLSETG KILPRRRTGL SAKEQRILAK TIKRARILGL LPFTEKLVRK

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Macromolecule #19: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 9.006486 KDa
SequenceString:
SLKKGVFVDD HLLEKVLELN AKGEKRLIKT WSRRSTIVPE MVGHTIAVYN GKQHVPVYIT ENMVGHKLGE FAPTRTYRG

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Macromolecule #20: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 10.921086 KDa
SequenceString:
RNLSALKRHR QSLKRRLRNK AKKSAIKTLS KKAIQLAQEG KAEEALKIMR KAESLIDKAA KGSTLHKNAA ARRKSRLMRK VRQLLEAAG APLIGGGLSA

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Macromolecule #21: 30S ribosomal protein Thx

MacromoleculeName: 30S ribosomal protein Thx / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 3.089655 KDa
SequenceString:
GKGDRRTRRG KIWRGTYGKY RPRKK

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Macromolecule #25: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 9.529074 KDa
SequenceString:
MAHKKGLGST RNGRDSQAKR LGVKRYEGQV VRAGNILVRQ RGTRFKPGKN VGMGRDFTLF ALVDGVVEFQ DRGRLGRYVH VRPLA

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Macromolecule #26: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 9.985846 KDa
SequenceString:
SGKRPIVANS IQRRGKAKRE GGVGKKTTGI SKRRQYPNLQ KVRVRVAGQE ITFRVAASHI PKVYELVERA KGLKLEGLSP KEIKKELLK

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Macromolecule #27: 50S ribosomal protein L29

MacromoleculeName: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 6.112247 KDa
SequenceString:
EARKLSPVEL EKLVREKKRE LMELRFQASI GQLSQNHKIR DLKRQIARLL T

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Macromolecule #28: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 6.799126 KDa
SequenceString:
MPRLKVKLVK SPIGYPKDQK AALKALGLRR LQQERVLEDT PAIRGNVEKV AHLVRVEVVE

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Macromolecule #29: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 6.640646 KDa
SequenceString:
KEGIHPKLVP ARIICGCGNV IETYSTKPEI YVEVCSKCHP FYTGQQRFVD TEGRVERF

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Macromolecule #30: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 6.590854 KDa
SequenceString:
AKHPVPKKKT SKARRDARRS HHALTPPTLV PCPECKAMKP PHTVCPECGY YAGRKVLEV

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Macromolecule #31: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 5.602612 KDa
SequenceString:
LLLECTECKR RNYATEKNKR NTPNKLELRK YCPWCRKHTV HREVK

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Macromolecule #32: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 6.132449 KDa
SequenceString:
MKRTWQPNRR KRAKTHGFRA RMRTPGGRKV LKRRRQKGRW RLTPAVRKR

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Macromolecule #33: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 7.374981 KDa
SequenceString:
PKMKTHKGAK KRVKITASGK VVAMKTGKRH LNWQKSGKEI RQKGRKFVLA KPEAERIKLL LPYE

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Macromolecule #36: 50S ribosomal protein L1

MacromoleculeName: 50S ribosomal protein L1 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 24.872721 KDa
SequenceString: MPKHGKRYRA LLEKVDPNKV YTIDEAARLV KELATAKFDE TVEVHAKLGI DPRRSDQNVR GTVSLPHGLG KQVRVLAIAK GEKIKEAEE AGADYVGGEE IIQKILDGWM DFDAVVATPD VMGAVGSKLG RILGPRGLLP NPKAGTVGFN IGEIIREIKA G RIEFRNDK ...String:
MPKHGKRYRA LLEKVDPNKV YTIDEAARLV KELATAKFDE TVEVHAKLGI DPRRSDQNVR GTVSLPHGLG KQVRVLAIAK GEKIKEAEE AGADYVGGEE IIQKILDGWM DFDAVVATPD VMGAVGSKLG RILGPRGLLP NPKAGTVGFN IGEIIREIKA G RIEFRNDK TGAIHAPVGK ASFPPEKLAD NIRAFIRALE AHKPEGAKGT FLRSVYVTTT MGPSVRINPH S

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Macromolecule #37: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 29.985799 KDa
SequenceString: AVKKFKPYTP SRRFMTVADF SEITKTEPEK SLVKPLKKTG GRNNQGRITV RFRGGGHKRL YRIIDFKRWD KVGIPAKVAA IEYDPNRSA RIALLHYVDG EKRYIIAPDG LQVGQQVVAG PDAPIQVGNA LPLRFIPVGT VVHAVELEPK KGAKLARAAG T SAQIQGRE ...String:
AVKKFKPYTP SRRFMTVADF SEITKTEPEK SLVKPLKKTG GRNNQGRITV RFRGGGHKRL YRIIDFKRWD KVGIPAKVAA IEYDPNRSA RIALLHYVDG EKRYIIAPDG LQVGQQVVAG PDAPIQVGNA LPLRFIPVGT VVHAVELEPK KGAKLARAAG T SAQIQGRE GDYVILRLPS GELRKVHGEC YATVGAVGNA DHKNIVLGKA GRSRWLGRRP HVRGAAMNPV DHPHGGGEGR AP RGRPPAS PWGWQTKGLK TRKRRKPSSR FIIAR

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Macromolecule #38: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 22.321031 KDa
SequenceString: MKGILGVKVG MTRIFRDDRA VPVTVILAGP CPVVQRRTPE KDGYTAVQLG FLPQNPKRVN RPLKGHFAKA GVEPVRILRE IRDFNPEGD TVTVEIFKPG ERVDVTGTSK GRGFAGVMKR WNFAGGPDSH GAHKIHRHPG SIGNRKTPGR VYKGKKMAGH Y GAERVTVM ...String:
MKGILGVKVG MTRIFRDDRA VPVTVILAGP CPVVQRRTPE KDGYTAVQLG FLPQNPKRVN RPLKGHFAKA GVEPVRILRE IRDFNPEGD TVTVEIFKPG ERVDVTGTSK GRGFAGVMKR WNFAGGPDSH GAHKIHRHPG SIGNRKTPGR VYKGKKMAGH Y GAERVTVM NLEVVDVIPE ENLLLVKGAV PGPNGGLVIV RETKKAA

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Macromolecule #39: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 23.071721 KDa
SequenceString: MKEVAVYQIP VLSPSGRREL AADLPAEINP HLLWEVVRWQ LAKRRRGTAS TKTRGEVAYS GRKIWPQKHT GRARHGDIGA PIFVGGGVV FGPKPRDYSY TLPKKVRKKG LAMAVADRAR EGKLLLVEAF AGVNGKTKEF LAWAKEAGLD GSESVLLVTG N ELVRRAAR ...String:
MKEVAVYQIP VLSPSGRREL AADLPAEINP HLLWEVVRWQ LAKRRRGTAS TKTRGEVAYS GRKIWPQKHT GRARHGDIGA PIFVGGGVV FGPKPRDYSY TLPKKVRKKG LAMAVADRAR EGKLLLVEAF AGVNGKTKEF LAWAKEAGLD GSESVLLVTG N ELVRRAAR NLPWVVTLAP EGLNVYDIVR TERLVMDLDA WEVFQNRIGG

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Macromolecule #40: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 20.9304 KDa
SequenceString:
PLDVALKRKY YEEVRPELIR RFGYQNVWEV PRLEKVVINQ GLGEAKEDAR ILEKAAQELA LITGQKPAVT RAKKSISNFK LRKGMPIGL RVTLRRDRMW IFLEKLLNVA LPRIRDFRGL NPNSFDGRGN YNLGLREQLI FPEITYDMVD ALRGMDIAVV T TAETDEEA RALLELLGFP FRK

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Macromolecule #41: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 17.476279 KDa
SequenceString:
PKGVSVEVAP GRVKVKGPKG ELEVPVSPEM RVVVEEGVVR VERPSDERRH KSLHGLTRTL IANAVKGVSE GYSKELLIKG IGYRARLVG RALELTVGFS HPVVVEPPEG ITFEVPEPTR VRVSGIDKQK VGQVAANIRA IRKPSAYHEK GIYYAGEPVR L

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Macromolecule #42: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 6.536709 KDa
SequenceString:
MKVILLEPLE NLGDVGQVVD VKPGYARNYL LPRGLAVLAT ESNLKALEAR IRAQAKRLAA

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Macromolecule #43: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 15.828771 KDa
SequenceString:
MKTYVPKQVE PRWVLIDAEG KTLGRLATKI ATLLRGKHRP DWTPNVAMGD FVVVVNADKI RVTGKKLEQK IYTRYSGYPG GLKKIPLEK MLATHPERVL EHAVKGMLPK GPLGRRLFKR LKVYAGPDHP HQAQRPEKLE

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Macromolecule #44: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 13.323612 KDa
SequenceString:
MIQPQTYLEV ADNTGARKIM CIRVLKGSNA KYATVGDVIV ASVKEAIPRG AVKEGDVVKA VVVRTKKEIK RPDGSAIRFD DNAAVIINN QLEPRGTRVF GPVARELREK GFMKIVSLAP EVL

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Macromolecule #45: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 15.858526 KDa
SequenceString:
DLRPNPGANK RRKRVGRGPG SGHGKTATRG HKGQKSRSGG LKDPRRFEGG RSTTLMRLPK RGMQGQVPGE IKRPRYQGVN LKDLARFEG EVTPELLVRA GLLKKGYRLK ILGEGEAKPL KVVAHAFSKS ALEKLKAAGG EPVLLEA

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Macromolecule #46: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 15.36405 KDa
SequenceString:
RMKYRKQQRG RLKGATKGGD YVAFGDYGLV ALEPAWITAQ QIEAARVAMV RHFRRGGKIF IRIFPDKPYT KKPLEVRMGK GKGNVEGYV AVVKPGRVMF EVAGVTEEQA MEALRIAGHK LPIKTKIVRR DAYDEAQ

+
Macromolecule #47: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 13.618952 KDa
SequenceString:
RHLKSGRKLN RHSSHRLALY RNQAKSLLTH GRITTTVPKA KELRGFVDHL IHLAKRGDLH ARRLVLRDLQ DVKLVRKLFD EIAPRYRDR QGGYTRVLKL AERRRGDGAP LALVELVE

+
Macromolecule #48: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 10.998918 KDa
SequenceString:
KFRVRNRIKR TGRLRLSVFR SLKHIYAQII DDEKGVTLVS ASSLALKLKG NKTEVARQVG RALAEKALAL GIKQVAFDRG PYKYHGRVK ALAEGAREGG

+
Macromolecule #49: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 16.289891 KDa
SequenceString:
MNRGALIKLV ESRYVRTDLP EFRPGDTVRV SYKVKEGNRT RIQDFEGIVI RIRRNGFNTT FTVRKVSYGV GVERIFPLHS PLIQKIDIV QRGRARRAKL YFIRNLSDRE IRRKLRADRK RIDQDRAAER AAKEEAQKA

+
Macromolecule #50: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 13.648081 KDa
SequenceString:
PRAKTGVVRR RKHKKILKLA KGYWGLRSKS FRKARETLFA AGNYAYAHRK RRKRDFRRLW IVRINAACRQ HGLNYSTFIH GLKKAGIEV DRKNLADLAV REPQVFAELV ERAKAAQG

+
Macromolecule #51: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.069153 KDa
SequenceString:
MFAIVKTGGK QYRVEPGLKL RVEKLDAEPG ATVELPVLLL GGEKTVVGTP VVEGASVVAE VLGHGRGKKI LVSKFKAKVQ YRRKKGHRQ PYTELLIKEI RG

+
Macromolecule #52: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 12.808055 KDa
SequenceString:
MEAKAIARYV RISPRKVRLV VDLIRGKSLE EARNILRYTN KRGAYFVAKV LESAAANAVN NHDMLEDRLY VKAAYVDEGP ALKRVLPRA RGRADIIKKR TSHITVILGE KHGK

+
Macromolecule #53: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 10.386274 KDa
SequenceString:
TAYDVILAPV LSEKAYAGFA EGKYTFWVHP KATKTEIKNA VETAFKVKVV KVNTLHVRGK KKRLGRYLGK RPDRKKAIVQ VAPGQKIEA LEGL

+
Macromolecule #54: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.181658 KDa
SequenceString:
RVKMHVKKGD TVLVASGKYK GRVGKVKEVL PKKYAVIVEG VNIVKKAVRV SPKYPQGGFI EKEAPLHASK VRPICPACGK PTRVRKKFL ENGKKIRVCA KC

+
Macromolecule #55: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 10.954641 KDa
SequenceString:
YRLKAYYREG EKPSALRRAG KLPGVMYNRH LNRKVYVDLV EFDKVFRQAS IHHVIVLELP DGQSLPTLVR QVNLDKRRRR PEHVDFFVL SDD

+
Macromolecule #56: SsrA-binding protein

MacromoleculeName: SsrA-binding protein / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 16.78549 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MAPVLENRRA RHDYEILETY EAGIALKGTE VKSLRAGKVD FTGSFARFED GELYLENLYI APYEKGSYAN VDPRRKRKLL LHKHELRRL LGKVEQKGLT LVPLKIYFNE RGYAKVLLGL ARGKKAYEKR REDKKEAVRR ALEEL

+
Macromolecule #57: Nascent peptide MET-ALA

MacromoleculeName: Nascent peptide MET-ALA / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 220.289 Da
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MA

+
Macromolecule #58: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) (bacteria)
Molecular weightTheoretical: 4.435411 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MKVRASVKRI CDKCKVIRRH GRVYVICENP KHKQRQG

+
Macromolecule #59: 50S RIBOSOMAL PROTEIN L10 AND L11

MacromoleculeName: 50S RIBOSOMAL PROTEIN L10 AND L11 / type: protein_or_peptide / ID: 59 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) (bacteria)
Molecular weightTheoretical: 11.932701 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

+
Macromolecule #61: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 61 / Number of copies: 637 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #62: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 62 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI POLARA 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 42.0 e/Å2
Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company

-
Image processing

CTF correctionSoftware - Name: Gctf
Startup modelType of model: EMDB MAP
EMDB ID:

3493

Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 24400

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