ジャーナル: Elife / 年: 2014 タイトル: Structure of catalase determined by MicroED. 著者: Brent L Nannenga / Dan Shi / Johan Hattne / Francis E Reyes / Tamir Gonen / 要旨: MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of ...MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of still diffraction patterns to determine the structure of lysozyme at 2.9 Å resolution with MicroED (Shi et al., 2013). Here we present the structure of bovine liver catalase determined from a single crystal at 3.2 Å resolution by MicroED. The data were collected by continuous rotation of the sample under constant exposure and were processed and refined using standard programs for X-ray crystallography. The ability of MicroED to determine the structure of bovine liver catalase, a protein that has long resisted atomic analysis by traditional electron crystallography, demonstrates the potential of this method for structure determination.
解像度: 3.2 Å / 解像度の算出法: DIFFRACTION PATTERN/LAYERLINES / 対称性のタイプ: 3D CRYSTAL
精密化
解像度: 3.2→125.06 Å / Cor.coef. Fo:Fc: 0.795 / Cor.coef. Fo:Fc free: 0.745 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R Free: 0.853 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.3079
1369
4.9 %
RANDOM
Rwork
0.2621
26732
-
-
obs
0.2644
28101
77.97 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK