EMDB-3683: Structure of a pre-catalytic spliceosome (B2 map)

Basic information

• Entry: Database: EMDB / ID: EMD-3683
• Title: Structure of a pre-catalytic spliceosome (B2 map)
• Map data: unsharpened B2 map

Sample

• Complex: Pre-catalytic B complex Spliceosome
  • RNA: x 5 types
  • Protein or peptide: x 39 types
• Ligand: x 2 types

Function / homology

Function:

Sm-like protein family complex / spliceosomal conformational changes to generate catalytic conformation / mRNA decay by 5' to 3' exoribonuclease / snoRNA splicing / maturation of 5S rRNA / snoRNA guided rRNA 2'-O-methylation / Lsm1-7-Pat1 complex / U6 snRNP / box C/D sno(s)RNA 3'-end processing / deadenylation-dependent decapping of nuclear-transcribed mRNA / generation of catalytic spliceosome for first transesterification step / box C/D methylation guide snoRNP complex / splicing factor binding / U4/U6 snRNP / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / 7-methylguanosine cap hypermethylation / pICln-Sm protein complex / P-body assembly / sno(s)RNA-containing ribonucleoprotein complex / snRNP binding / small nuclear ribonucleoprotein complex / U4 snRNA binding / SMN-Sm protein complex / spliceosomal tri-snRNP complex / U2-type spliceosomal complex / mRNA cis splicing, via spliceosome / poly(U) RNA binding / commitment complex / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / U3 snoRNA binding / U1 snRNP / U2-type prespliceosome / tRNA processing / precatalytic spliceosome / Major pathway of rRNA processing in the nucleolus and cytosol / generation of catalytic spliceosome for second transesterification step / spliceosomal complex assembly / mRNA 5'-splice site recognition / nuclear-transcribed mRNA catabolic process / mRNA 3'-splice site recognition / spliceosomal tri-snRNP complex assembly / U5 snRNA binding / U5 snRNP / U2 snRNA binding / U6 snRNA binding / spliceosomal snRNP assembly / cellular response to glucose starvation / pre-mRNA intronic binding / U1 snRNA binding / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / spliceosomal complex / P-body / mRNA splicing, via spliceosome / rRNA processing / metallopeptidase activity / nucleic acid binding / RNA helicase activity / RNA helicase / response to xenobiotic stimulus / GTPase activity / mRNA binding / nucleolus / GTP binding / ATP hydrolysis activity / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / ATP binding / identical protein binding / nucleus / metal ion binding / cytosol / cytoplasm

Domain/homology:

Pre-mRNA-splicing factor Prp9, N-terminal / Pre-mRNA-splicing factor PRP9 N-terminus / U4/U6.U5 small nuclear ribonucleoprotein component Snu23 / Pre-mRNA-splicing factor 38 / PRP38 family / SNU66/SART1 family / HIND motif / SART-1 family / HIND motif / Sm-like protein Lsm8 / U6 snRNA-associated Sm-like protein Lsm1/8 / Pre-mRNA processing factor 4 (PRP4)-like / U6 snRNA-associated Sm-like protein LSm2 / Sm-like protein Lsm4 / Splicing Factor Motif, present in Prp18 and Pr04 / Sm-like protein Lsm7 / Sm-like protein LSm5 / Sm-like protein Lsm3 / U6 snRNA-associated Sm-like protein Lsm3 / RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) / Pre-mRNA-splicing factor 3 / U4/U6 small nuclear ribonucleoprotein Prp3 / pre-mRNA processing factor 3 domain / Prp31 C-terminal / U4/U6 small nuclear ribonucleoprotein Prp31 / Prp31 C terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / Dim1 family / Mitosis protein DIM1 / Mitosis protein DIM1 / PRP1 splicing factor, N-terminal / SF3A2 domain / : / PRP1 splicing factor, N-terminal / Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11) / Splicing factor SF3a60 /Prp9 subunit, C-terminal / SF3A3 domain / SF3a60/Prp9 C-terminal / Pre-mRNA-splicing factor SF3A3, of SF3a complex, Prp9 / SF3B4, RNA recognition motif 1 / : / Splicing factor 3A subunit 1, conserved domain / Splicing factor 3A subunit 1 / Pre-mRNA splicing factor PRP21 like protein / : / : / Domain of unknown function DUF382 / Domain of unknown function (DUF382) / SWAP/Surp / SWAP/Surp superfamily / Surp module / SURP motif repeat profile. / Suppressor-of-White-APricot splicing regulator / Zinc-finger of C2H2 type / PHF5-like / PHF5-like protein / PSP, proline-rich / PSP / proline-rich domain in spliceosome associated proteins / Splicing factor 3B subunit 5/RDS3 complex subunit 10 / Splicing factor 3B subunit 10 (SF3b10) / Splicing factor 3B subunit 1-like / Matrin/U1-C, C2H2-type zinc finger / Zinc finger matrin-type profile. / Zinc-finger double-stranded RNA-binding / Zinc finger, double-stranded RNA binding / H/ACA ribonucleoprotein complex, subunit Nhp2-like / NOSIC / NOSIC (NUC001) domain / Nop domain / Nop domain superfamily / Nop, C-terminal domain / snoRNA binding domain, fibrillarin / Nop domain profile. / U2A'/phosphoprotein 32 family A, C-terminal / occurring C-terminal to leucine-rich repeats / PPP2R1A-like HEAT repeat / Brr2, N-terminal helicase PWI domain / : / N-terminal helicase PWI domain / Pre-mRNA-splicing helicase BRR2 plug domain / Sec63 Brl domain / : / Leucine-rich repeat / Pre-mRNA-splicing factor Syf1-like / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / Snu114, GTP-binding domain / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Sec63 domain / Sec63 Brl domain / Small nuclear ribonucleoprotein Sm D3 / : / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Sm-like protein Lsm7/SmG

Component:

U6 snRNA-associated Sm-like protein LSm6 / RDS3 complex subunit 10 / Pre-mRNA-splicing factor 6 / Pre-mRNA-splicing factor PRP9 / U4/U6 small nuclear ribonucleoprotein PRP4 / Pre-mRNA-splicing factor PRP21 / Pre-mRNA-splicing helicase BRR2 / Pre-mRNA-splicing factor 8 / Pre-mRNA-splicing factor SNU114 / U6 snRNA-associated Sm-like protein LSm2 / Pre-mRNA-splicing factor SPP381 / 13 kDa ribonucleoprotein-associated protein / Small nuclear ribonucleoprotein-associated protein B / U6 snRNA-associated Sm-like protein LSm4 / U6 snRNA-associated Sm-like protein LSm5 / Small nuclear ribonucleoprotein G / U2 small nuclear ribonucleoprotein B'' / Small nuclear ribonucleoprotein Sm D3 / U6 snRNA-associated Sm-like protein LSm8 / Pre-mRNA-processing factor 31 / U2 snRNP component HSH155 / U6 snRNA-associated Sm-like protein LSm7 / Small nuclear ribonucleoprotein F / U6 snRNA-associated Sm-like protein LSm3 / Pre-mRNA-splicing factor 38 / Small nuclear ribonucleoprotein Sm D1 / Cold sensitive U2 snRNA suppressor 1 / U4/U6 small nuclear ribonucleoprotein PRP3 / Pre-mRNA-splicing factor RSE1 / Small nuclear ribonucleoprotein Sm D2 / Spliceosomal protein DIB1 / Pre-mRNA-splicing factor RDS3 / Pre-mRNA-splicing factor PRP11 / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein E / 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component / 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component / Protein HSH49

• Biological species: Saccharomyces cerevisiae (brewer's yeast) / Baker's yeast (brewer's yeast)
• Method: single particle reconstruction / cryo EM / Resolution: 7.2 Å
• Authors: Plaschka C / Lin P-C / Nagai K

Funding support

United Kingdom, 2 items

Organization	Grant number	Country
Medical Research Council (United Kingdom)	MC_U105184330	United Kingdom
European Research Council	693087 - SPLICE3D	United Kingdom

• Citation: Journal: Nature / Year: 2017; Title: Structure of a pre-catalytic spliceosome.; Authors: Clemens Plaschka / Pei-Chun Lin / Kiyoshi Nagai / ; Abstract: Intron removal requires assembly of the spliceosome on precursor mRNA (pre-mRNA) and extensive remodelling to form the spliceosome's catalytic centre. Here we report the cryo-electron microscopy structure of the yeast Saccharomyces cerevisiae pre-catalytic B complex spliceosome at near-atomic resolution. The mobile U2 small nuclear ribonucleoprotein particle (snRNP) associates with U4/U6.U5 tri-snRNP through the U2/U6 helix II and an interface between U4/U6 di-snRNP and the U2 snRNP SF3b-containing domain, which also transiently contacts the helicase Brr2. The 3' region of the U2 snRNP is flexibly attached to the SF3b-containing domain and protrudes over the concave surface of tri-snRNP, where the U1 snRNP may reside before its release from the pre-mRNA 5' splice site. The U6 ACAGAGA sequence forms a hairpin that weakly tethers the 5' splice site. The B complex proteins Prp38, Snu23 and Spp381 bind the Prp8 N-terminal domain and stabilize U6 ACAGAGA stem-pre-mRNA and Brr2-U4 small nuclear RNA interactions. These results provide important insights into the events leading to active site formation.

History

Deposition	Apr 24, 2017	-
Header (metadata) release	May 31, 2017	-
Map release	May 31, 2017	-
Update	May 24, 2023	-
Current status	May 24, 2023	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_3683.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: unsharpened B2 map
• Voxel size: X=Y=Z: 1.43 Å

Density

Contour Level	By AUTHOR: 0.0258 / Movie #1: 0.0258
Minimum - Maximum	-0.01883009 - 0.080454715
Average (Standard dev.)	-7.166218e-05 (±0.0032017468)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 480 480 480 Spacing 480 480 480
Cell	A=B=C: 686.39996 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.43	1.43	1.43
M x/y/z	480	480	480
origin x/y/z	0.000	0.000	0.000
length x/y/z	686.400	686.400	686.400
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	0	0	0
NX/NY/NZ	450	450	450
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	480	480	480
D min/max/mean	-0.019	0.080	-0.000

Supplemental data

Additional map: sharpened B2 map

• File: emd_3683_additional.map
• Annotation: sharpened B2 map

Sample components

Entire : Pre-catalytic B complex Spliceosome

• Entire: Name: Pre-catalytic B complex Spliceosome

Components

• Complex: Pre-catalytic B complex Spliceosome
  • RNA: U2 snRNA
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm3
  • RNA: U4 snRNA
  • RNA: U5 snRNA
  • RNA: U6 snRNA
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm7
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm8
  • Protein or peptide: Pre-mRNA-splicing factor 8
  • Protein or peptide: Pre-mRNA-splicing helicase BRR2
  • Protein or peptide: Pre-mRNA-splicing factor SNU114
  • Protein or peptide: Spliceosomal protein DIB1
  • Protein or peptide: 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component
  • Protein or peptide: Pre-mRNA-processing factor 31
  • Protein or peptide: U4/U6 small nuclear ribonucleoprotein PRP3
  • Protein or peptide: U4/U6 small nuclear ribonucleoprotein PRP4
  • RNA: Yeast UBC4 gene for ubiquitin-conjugating enzyme
  • Protein or peptide: Pre-mRNA-splicing factor 6
  • Protein or peptide: 13 kDa ribonucleoprotein-associated protein
  • Protein or peptide: 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component
  • Protein or peptide: Pre-mRNA-splicing factor 38
  • Protein or peptide: Pre-mRNA-splicing factor SPP381
  • Protein or peptide: U2 snRNP component HSH155
  • Protein or peptide: Pre-mRNA-splicing factor RSE1
  • Protein or peptide: Cold sensitive U2 snRNA suppressor 1
  • Protein or peptide: Protein HSH49
  • Protein or peptide: Pre-mRNA-splicing factor RDS3
  • Protein or peptide: Pre-mRNA-splicing factor PRP9
  • Protein or peptide: Pre-mRNA-splicing factor PRP11
  • Protein or peptide: Pre-mRNA-splicing factor PRP21
  • Protein or peptide: U2 small nuclear ribonucleoprotein A'
  • Protein or peptide: Unknown
  • Protein or peptide: U2 small nuclear ribonucleoprotein B''
  • Protein or peptide: RDS3 complex subunit 10
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm2
  • Protein or peptide: Small nuclear ribonucleoprotein-associated protein B
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
  • Protein or peptide: Small nuclear ribonucleoprotein E
  • Protein or peptide: Small nuclear ribonucleoprotein F
  • Protein or peptide: Small nuclear ribonucleoprotein G
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm4
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm5
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm6
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: ZINC ION

Supramolecule #1: Pre-catalytic B complex Spliceosome

• Supramolecule: Name: Pre-catalytic B complex Spliceosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: BCY123
• Molecular weight: Theoretical: 2.5 MDa

Macromolecule #1: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 1 / Details: Saccharomyces cerevisiae U2 snRNA / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 376.267406 KDa

Sequence

String:

ACGAAUCUCU UUGCCUUUUG GCUUAGAUCA AGUG(PSU)AGUAU C(PSU)G(PSU)UCUUUU CAGUGUAACA ACUGAAAU G ACCUCAAUGA GGCUCAUUAC CUUUUAAUUU GUUACAAUAC ACAUUUUUUG GCACCCAAAA UAAUAAAAUG GACGGGAAG AGACUUUUUA AGCAAGUUGU UUUCCGCUAA UGUCAGGUCU CACUACUUUU UGCUGCUAUU UUUCUUCGCU CAUGGUUUCU UCAUAAGGC GUUUUUAUGA UGGUUUUUCG AAAUUGGUUU UUGAGACGAC GGUUGCUCAA GGUUAUUGUU UUUGUUUUCU U CUGGUUGU UUUCUAUUUU CUUUUUUUUA GCUUUCUGUU UCUCCCUUAG UUUGGCUUUU UGCUUCAUAC UCUUCCCUGU CU UUCCGAG CCGUUUAUGU CCAACGCGGG AUUUGGUUUU UCUUUAUCGA UGGGAAGAAA UGGUGCUAUA GUAGGUUGGG AGA UAAUAU UUAUGGUAUG GGGUGCUAGU GCGGAUGGGG CGCUCUUAUU GUUGAUUUCU UCGCUCGUCU UCUUUUUCUG GUGG CGCUG CAAGAGGAAG UUUUUCGACU UUGUUAUGAU UUUUGGUUUG CAAGGAAAGG UGUCUUACGA UUCUUUUUUU GAUGU AAUA GGAUAAGCUU GCUUAUCCCC CAAGUAUCGG CCAAAGUUGU UGAUUUUCCU UUUGAAGUGU CCUCGGUUUG AGGGGG UGU AGGGUGGGGU UGGUCUACAA UAAGAGUGUU CCAUUGUUAA CGUGCUGGCG UCUUUUACUA UAUUUUUUUU CCCAGUU UA UUUUGUGCUU AUUUUCUCAU UGAGGAGAAG GAGCUCUUCU CGCAGGAUAU AAAUGGAGGU UUGCUAAAGG GGAGGAGA U GUGUUUGUGA GAAUACUGCU GAGAGAGUUC UGGAAGAGAA AAAAAGGAGG CAAUGGAAGG CGUUUGCUGG GAAAAGAGA AGAGCCAUGA CUGCAUCUGU UGUUUCAAGG CCAGUUUUAU UAACCGCCUA UGUCAUAGAG GCGUUUUUUU UGGAGGGAUU UGAAGAAUG CCGGCGGCAU CAAGAAACGG ACUUGAUGGU UGACGCCUGU UUUUAAAGUU AGAGACGUCG CGACCCUCGC A CUUGUGGA GUCGUUCUUG ACUUUUACUU UGGUCGCUUG AUGUUUCUCU CGUCUUCCCG UUCGCUCUU

Macromolecule #3: U4 snRNA

• Macromolecule: Name: U4 snRNA / type: rna / ID: 3 / Details: Saccharomyces cerevisiae U4 snRNA / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 51.186023 KDa

Sequence

String:

AUCCUUAUGC ACGGGAAAUA CGCAUAUCAG UGAGGAUUCG UCCGAGAUUG UGUUUUUGCU GGUUGAAAUU UAAUUAUAAA CCAGACCGU CUCCUCAUGG UCAAUUCGGU GUUCGCUUUU GAAUACUUCA AGACUAUGUA GGGAAUUUUU GGAAUACCUU U

Macromolecule #4: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 4 / Details: Saccharomyces cerevisiae U5 snRNA / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 68.643344 KDa

Sequence

String:

AAGCAGCUUU ACAGAUCAAU GGCGGAGGGA GGUCAACAUC AAGAACUGUG GGCCUUUUAU UGCCUAUAGA ACUUAUAACG AACAUGGUU CUUGCCUUUU ACCAGAACCA UCCGGGUGUU GUCUCCAUAG AAACAGGUAA AGCUGUCCGU UACUGUGGGC U UGCCAUAU UUUUUGGAAC UUUUCUGCCC UUUUUCUCAA UGAGUAAGGA GGGCGU

Macromolecule #5: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 5 / Details: Saccharomyces cerevisiae U6 snRNA / Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 35.883176 KDa

Sequence

String:

GUUCGCGAAG UAACCCUUCG UGGACAUUUG GUCAAUUUGA AACAAUACAG AGAUGAUCAG CAGUUCCCCU GCAUAAGGAU GAACCGUUU UACAAAGAGA UUUAUUUCGU UUU

Macromolecule #16: Yeast UBC4 gene for ubiquitin-conjugating enzyme

• Macromolecule: Name: Yeast UBC4 gene for ubiquitin-conjugating enzyme / type: rna / ID: 16 ; Details: Saccharomyces cerevisiae UBC4 pre-mRNA for ubiquitin-conjugating enzyme, in vitro transcribed.; Number of copies: 1
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 30.20073 KDa

Sequence

String:

GUAUGUCUAA AGUUAUGGCC ACGUUUCAAA UGCGUGCUUU UUUUUUAAAA CUUAUGCUCU UAUUUACUAA CAAAAUCAAC AUGCUAUUG AACUAG

Macromolecule #2: U6 snRNA-associated Sm-like protein LSm3

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm3 / type: protein_or_peptide / ID: 2 / Details: U6 snRNA-associated Sm-like protein LSm3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 10.039262 KDa

Sequence

String:

METPLDLLKL NLDERVYIKL RGARTLVGTL QAFDSHCNIV LSDAVETIYQ LNNEELSESE RRCEMVFIRG DTVTLISTPS EDDDGAVEI

Macromolecule #6: U6 snRNA-associated Sm-like protein LSm7

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm7 / type: protein_or_peptide / ID: 6 / Details: U6 snRNA-associated Sm-like protein LSm7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 13.027045 KDa

Sequence

String:

MHQQHSKSEN KPQQQRKKFE GPKREAILDL AKYKDSKIRV KLMGGKLVIG VLKGYDQLMN LVLDDTVEYM SNPDDENNTE LISKNARKL GLTVIRGTIL VSLSSAEGSD VLYMQK

Macromolecule #7: U6 snRNA-associated Sm-like protein LSm8

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm8 / type: protein_or_peptide / ID: 7 / Details: U6 snRNA-associated Sm-like protein LSm8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 12.403378 KDa

Sequence

String:

MSATLKDYLN KRVVIIKVDG ECLIASLNGF DKNTNLFITN VFNRISKEFI CKAQLLRGSE IALVGLIDAE NDDSLAPIDE KKVPMLKDT KNKIENEHVI WEKVYESKTK

Macromolecule #8: Pre-mRNA-splicing factor 8

• Macromolecule: Name: Pre-mRNA-splicing factor 8 / type: protein_or_peptide / ID: 8 / Details: Pre-mRNA-splicing factor 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 279.867469 KDa

Sequence

String:

MSGLPPPPPG FEEDSDLALP PPPPPPPGYE IEELDNPMVP SSVNEDTFLP PPPPPPSNFE INAEEIVDFT LPPPPPPPGL DELETKAEK KVELHGKRKL DIGKDTFVTR KSRKRAKKMT KKAKRSNLYT PKAEMPPEHL RKIINTHSDM ASKMYNTDKK A FLGALKYL PHAILKLLEN MPHPWEQAKE VKVLYHTSGA ITFVNETPRV IEPVYTAQWS ATWIAMRREK RDRTHFKRMR FP PFDDDEP PLSYEQHIEN IEPLDPINLP LDSQDDEYVK DWLYDSRPLE EDSKKVNGTS YKKWSFDLPE MSNLYRLSTP LRD EVTDKN YYYLFDKKSF FNGKALNNAI PGGPKFEPLY PREEEEDYNE FNSIDRVIFR VPIRSEYKVA FPHLYNSRPR SVRI PWYNN PVSCIIQNDE EYDTPALFFD PSLNPIPHFI DNNSSLNVSN TKENGDFTLP EDFAPLLAEE EELILPNTKD AMSLY HSPF PFNRTKGKMV RAQDVALAKK WFLQHPDEEY PVKVKVSYQK LLKNYVLNEL HPTLPTNHNK TKLLKSLKNT KYFQQT TID WVEAGLQLCR QGHNMLNLLI HRKGLTYLHL DYNFNLKPTK TLTTKERKKS RLGNSFHLMR ELLKMMKLIV DTHVQFR LG NVDAFQLADG IHYILNHIGQ LTGIYRYKYK VMHQIRACKD LKHIIYYKFN KNLGKGPGCG FWQPAWRVWL NFLRGTIP L LERYIGNLIT RQFEGRSNEI VKTTTKQRLD AYYDLELRNS VMDDILEMMP ESIRQKKART ILQHLSEAWR CWKANIPWD VPGMPAPIKK IIERYIKSKA DAWVSAAHYN RERIKRGAHV EKTMVKKNLG RLTRLWIKNE QERQRQIQKN GPEITPEEAT TIFSVMVEW LESRSFSPIP FPPLTYKNDT KILVLALEDL KDVYASKVRL NASEREELAL IEEAYDNPHD TLNRIKKYLL T QRVFKPVD ITMMENYQNI SPVYSVDPLE KITDAYLDQY LWYEADQRKL FPNWIKPSDS EIPPLLVYKW TQGINNLSEI WD VSRGQSA VLLETTLGEM AEKIDFTLLN RLLRLIVDPN IADYITAKNN VVINFKDMSH VNKYGLIRGL KFASFIFQYY GLV IDLLLL GQERATDLAG PANNPNEFMQ FKSKEVEKAH PIRLYTRYLD RIYMLFHFEE DEGEELTDEY LAENPDPNFE NSIG YNNRK CWPKDSRMRL IRQDVNLGRA VFWEIQSRVP TSLTSIKWEN AFVSVYSKNN PNLLFSMCGF EVRILPRQRM EEVVS NDEG VWDLVDERTK QRTAKAYLKV SEEEIKKFDS RIRGILMASG STTFTKVAAK WNTSLISLFT YFREAIVATE PLLDIL VKG ETRIQNRVKL GLNSKMPTRF PPAVFYTPKE LGGLGMISAS HILIPASDLS WSKQTDTGIT HFRAGMTHED EKLIPTI FR YITTWENEFL DSQRVWAEYA TKRQEAIQQN RRLAFEELEG SWDRGIPRIS TLFQRDRHTL AYDRGHRIRR EFKQYSLE R NSPFWWTNSH HDGKLWNLNA YRTDVIQALG GIETILEHTL FKGTGFNSWE GLFWEKASGF EDSMQFKKLT HAQRTGLSQ IPNRRFTLWW SPTINRANVY VGFLVQLDLT GIFLHGKIPT LKISLIQIFR AHLWQKIHES IVFDICQILD GELDVLQIES VTKETVHPR KSYKMNSSAA DITMESVHEW EVSKPSLLHE TNDSFKGLIT NKMWFDVQLR YGDYDSHDIS RYVRAKFLDY T TDNVSMYP SPTGVMIGID LAYNMYDAYG NWFNGLKPLI QNSMRTIMKA NPALYVLRER IRKGLQIYQS SVQEPFLNSS NY AELFNND IKLFVDDTNV YRVTVHKTFE GNVATKAING CIFTLNPKTG HLFLKIIHTS VWAGQKRLSQ LAKWKTAEEV SAL VRSLPK EEQPKQIIVT RKAMLDPLEV HMLDFPNIAI RPTELRLPFS AAMSIDKLSD VVMKATEPQM VLFNIYDDWL DRIS SYTAF SRLTLLLRAL KTNEESAKMI LLSDPTITIK SYHLWPSFTD EQWITIESQM RDLILTEYGR KYNVNISALT QTEIK DIIL GQNIKAPSVK RQKMAELEAA RSEKQNDEEA AGASTVMKTK TINAQGEEIV VVASADYESQ TFSSKNEWRK SAIANT LLY LRLKNIYVSA DDFVEEQNVY VLPKNLLKKF IEISDVKIQV AAFIYGMSAK DHPKVKEIKT VVLVPQLGHV GSVQISN IP DIGDLPDTEG LELLGWIHTQ TEELKFMAAS EVATHSKLFA DKKRDCIDIS IFSTPGSVSL SAYNLTDEGY QWGEENKD I MNVLSEGFEP TFSTHAQLLL SDRITGNFII PSGNVWNYTF MGTAFNQEGD YNFKYGIPLE FYNEMHRPVH FLQFSELAG DEELEAEQID VFS

Macromolecule #9: Pre-mRNA-splicing helicase BRR2

• Macromolecule: Name: Pre-mRNA-splicing helicase BRR2 / type: protein_or_peptide / ID: 9 / Details: Pre-mRNA-splicing helicase BRR2 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 246.470266 KDa

Sequence

String:

MTEHETKDKA KKIREIYRYD EMSNKVLKVD KRFMNTSQNP QRDAEISQPK SMSGRISAKD MGQGLCNNIN KGLKENDVAV EKTGKSASL KKIQQHNTIL NSSSDFRLHY YPKDPSNVET YEQILQWVTE VLGNDIPHDL IIGTADIFIR QLKENEENED G NIEERKEK IQHELGINID SLKFNELVKL MKNITDYETH PDNSNKQAVA ILADDEKSDE EEVTEMSNNA NVLGGEINDN ED DDEEYDY NDVEVNSKKK NKRALPNIEN DIIKLSDSKT SNIESVPIYS IDEFFLQRKL RSELGYKDTS VIQDLSEKIL NDI ETLEHN PVALEQKLVD LLKFENISLA EFILKNRSTI FWGIRLAKST ENEIPNLIEK MVAKGLNDLV EQYKFRETTH SKRE LDSGD DQPQSSEAKR TKFSNPAIPP VIDLEKIKFD ESSKLMTVTK VSLPEGSFKR VKPQYDEIHI PAPSKPVIDY ELKEI TSLP DWCQEAFPSS ETTSLNPIQS KVFHAAFEGD SNMLICAPTG SGKTNIALLT VLKALSHHYN PKTKKLNLSA FKIVYI APL KALVQEQVRE FQRRLAFLGI KVAELTGDSR LSRKQIDETQ VLVSTPEKWD ITTRNSNNLA IVELVRLLII DEIHLLH DD RGPVLESIVA RTFWASKYGQ EYPRIIGLSA TLPNYEDVGR FLRVPKEGLF YFDSSFRPCP LSQQFCGIKE RNSLKKLK A MNDACYEKVL ESINEGNQII VFVHSRKETS RTATWLKNKF AEENITHKLT KNDAGSKQIL KTEAANVLDP SLRKLIESG IGTHHAGLTR SDRSLSEDLF ADGLLQVLVC TATLAWGVNL PAHTVIIKGT DVYSPEKGSW EQLSPQDVLQ MLGRAGRPRY DTFGEGIII TDQSNVQYYL SVLNQQLPIE SQFVSKLVDN LNAEVVAGNI KCRNDAVNWL AYTYLYVRML ASPMLYKVPD I SSDGQLKK FRESLVHSAL CILKEQELVL YDAENDVIEA TDLGNIASSF YINHASMDVY NRELDEHTTQ IDLFRIFSMS EE FKYVSVR YEEKRELKQL LEKAPIPIRE DIDDPLAKVN VLLQSYFSQL KFEGFALNSD IVFIHQNAGR LLRAMFEICL KRG WGHPTR MLLNLCKSAT TKMWPTNCPL RQFKTCPVEV IKRLEASTVP WGDYLQLETP AEVGRAIRSE KYGKQVYDLL KRFP KMSVT CNAQPITRSV MRFNIEIIAD WIWDMNVHGS LEPFLLMLED TDGDSILYYD VLFITPDIVG HEFTLSFTYE LKQHN QNNL PPNFFLTLIS ENWWHSEFEI PVSFNGFKLP KKFPPPTPLL ENISISTSEL GNDDFSEVFE FKTFNKIQSQ VFESLY NSN DSVFVGSGKG TGKTAMAELA LLNHWRQNKG RAVYINPSGE KIDFLLSDWN KRFSHLAGGK IINKLGNDPS LNLKLLA KS HVLLATPVQF ELLSRRWRQR KNIQSLELMI YDDAHEISQG VYGAVYETLI SRMIFIATQL EKKIRFVCLS NCLANARD F GEWAGMTKSN IYNFSPSERI EPLEINIQSF KDVEHISFNF SMLQMAFEAS AAAAGNRNSS SVFLPSRKDC MEVASAFMK FSKAIEWDML NVEEEQIVPY IEKLTDGHLR APLKHGVGIL YKGMASNDER IVKRLYEYGA VSVLLISKDC SAFACKTDEV IILGTNLYD GAEHKYMPYT INELLEMVGL ASGNDSMAGK VLILTSHNMK AYYKKFLIEP LPTESYLQYI IHDTLNNEIA N SIIQSKQD CVDWFTYSYF YRRIHVNPSY YGVRDTSPHG ISVFLSNLVE TCLNDLVESS FIEIDDTEAE VTAEVNGGDD EA TEIISTL SNGLIASHYG VSFFTIQSFV SSLSNTSTLK NMLYVLSTAV EFESVPLRKG DRALLVKLSK RLPLRFPEHT SSG SVSFKV FLLLQAYFSR LELPVDFQND LKDILEKVVP LINVVVDILS ANGYLNATTA MDLAQMLIQG VWDVDNPLRQ IPHF NNKIL EKCKEINVET VYDIMALEDE ERDEILTLTD SQLAQVAAFV NNYPNVELTY SLNNSDSLIS GVKQKITIQL TRDVE PENL QVTSEKYPFD KLESWWLVLG EVSKKELYAI KKVTLNKETQ QYELEFDTPT SGKHNLTIWC VCDSYLDADK ELSFEI NVK

Macromolecule #10: Pre-mRNA-splicing factor SNU114

• Macromolecule: Name: Pre-mRNA-splicing factor SNU114 / type: protein_or_peptide / ID: 10 / Details: Pre-mRNA-splicing factor SNU114 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 114.174008 KDa

Sequence

String:

MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTP LVEPVTERTK LQEHTIFTQL KKNIPKTRYN RDYMLSMANI PERIINVGVI GPLHSGKTSL MDLLVIDSHK R IPDMSKNV ELGWKPLRYL DNLKQEIDRG LSIKLNGSTL LCTDLESKSR MINFLDAPGH VNFMDETAVA LAASDLVLIV ID VVEGVTF VVEQLIKQSI KNNVAMCFVI NKLDRLILDL KLPPMDAYLK LNHIIANINS FTKGNVFSPI DNNIIFASTK LGF TFTIKE FVSYYYAHSI PSSKIDDFTT RLWGSVYYHK GNFRTKPFEN VEKYPTFVEF ILIPLYKIFS YALSMEKDKL KNLL RSNFR VNLSQEALQY DPQPFLKHVL QLIFRQQTGL VDAITRCYQP FELFDNKTAH LSIPGKSTPE GTLWAHVLKT VDYGG AEWS LVRIYSGLLK RGDTVRILDT SQSESRQKRQ LHDISKTETS NEDEDEDDET PSCEVEEIGL LGGRYVYPVH EAHKGQ IVL IKGISSAYIK SATLYSVKSK EDMKQLKFFK PLDYITEAVF KIVLQPLLPR ELPKLLDALN KISKYYPGVI IKVEESG EH VILGNGELYM DCLLYDLRAS YAKIEIKISD PLTVFSESCS NESFASIPVS NSISRLGEEN LPGLSISVAA EPMDSKMI Q DLSRNTLGKG QNCLDIDGIM DNPRKLSKIL RTEYGWDSLA SRNVWSFYNG NVLINDTLPD EISPELLSKY KEQIIQGFY WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIY KTIKVAGTPL LEVRGQVPVI ESAGFETDLR LSTNGLGMCQ LYFWHKIWRK VPGDVLDKDA FIPKLKPAPI N SLSRDFVM KTRRRKGIST GGFMSNDGPT LEKYISAELY AQLRENGLVP

Macromolecule #11: Spliceosomal protein DIB1

• Macromolecule: Name: Spliceosomal protein DIB1 / type: protein_or_peptide / ID: 11 / Details: Spliceosomal protein DIB1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 16.798387 KDa

Sequence

String:

MASVLLPQLR TGWHVDQAIV TETKRLVVIR FGRKNDRQCM IMDELLSSIA ERVRNFAVIY LCDIDEVSDF DEMYELTDPM TVMFFYHNK HMMCDFGTGN NNKLNFIVDD KQEMIDILET IFRGARKNKG LVVSPYDYNH KRVS

Macromolecule #12: 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component

• Macromolecule: Name: 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component; type: protein_or_peptide / ID: 12 ; Details: 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 66.544047 KDa

Sequence

String:

MNKTENLSIE ETNEIREKLG MKPIPVFQEK NTDHKESLSI EETNELRASL GLKLIPPQQN FNSSPPNVHN TSKIDELREK ITKFQKKAN APLRMAHLLE ETNVNDDSSW LENMDAIPSS HESKRSSTLP RKGATKEDEN IDLHNVQVSY NIEALSPKKD T ILTLKESS IFDDTDSTEV LENVKAAEEN ADREKLRLRQ MNKDRRQKKK ILNVSSLDIE EEEEGEKHSI TTTHLIIGAE QG VMKAPNT ISAKPPTGKV KVNFDSANNM SDEDGGDFKP LKIKKRKIKD PRSTKARKSK ITDKMEIVKL VDEDESLSWM EEE QPVTII NPRTSSNNEL KGPEDLAREI EKARDEEKRR TESILKMREI SNSIVVDEKV TFLNTLDTSL SERSATENKV KVHG EGEKN IGDVTNGHTK EGSGNNTLTE AVNNEPNYEG DAENAPNFFS GLASTLGYLR KKSVFTTGDV DLKPGKDVNN SESLR RDVR NKEHTGTGTY TKDKLHGLEQ FTSSDSSNAN THSKRQDHYD PDIKLVYRDE KGNRLTTKEA YKKLSQKFHG TKSNKK KRA KMKSRIEARK NTPENGSLFE FDDN

Macromolecule #13: Pre-mRNA-processing factor 31

• Macromolecule: Name: Pre-mRNA-processing factor 31 / type: protein_or_peptide / ID: 13 / Details: Pre-mRNA-processing factor 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 56.382516 KDa

Sequence

String:

MSSEEDYFDE LEYDLADEVN EEKEDIQTKK LTTVNCQTEK FNPFEILPES IELFRTLALI SPDRLSLSET AQILPKIVDL KRILQQQEI DFIKLLPFFN EIIPLIKSNI KLMHNFLISL YSRRFPELSS LIPSPLQYSK VISILENENY SKNESDELFF H LENKAKLT REQILVLTMS MKTSFKNKEP LDIKTRTQIL EANSILENLW KLQEDIGQYI ASKISIIAPN VCFLVGPEIA AQ LIAHAGG VLEFSRIPSC NIASIGKNKH LSHELHTLES GVRQEGYLFA SDMIQKFPVS VHKQMLRMLC AKVSLAARVD AGQ KNGDRN TVLAHKWKAE LSKKARKLSE APSISETKAL PIPEDQPKKK RAGRKFRKYK EKFRLSHVRQ LQNRMEFGKQ EQTV LDSYG EEVGLGMSNT SLQQAVGATS GSRRSAGNQA KLTKVMKHRI SEANQQADEF LISLGHNTEQ PNLSPEMVQM HKKQH TNPE EETNWFSGHG

Macromolecule #14: U4/U6 small nuclear ribonucleoprotein PRP3

• Macromolecule: Name: U4/U6 small nuclear ribonucleoprotein PRP3 / type: protein_or_peptide / ID: 14 / Details: U4/U6 small nuclear ribonucleoprotein PRP3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 55.97432 KDa

Sequence

String:

MPPRNTYEKG NPKRQNSPYY KPSFLRREET TNDEEKFQGH GLKTELHSAL KSSNLNLIRR TYQTGENPYL SDPHDRGSSS RFNRRYERG LKFYQKGEIS KRIAQERTLQ KQQEEEELKR KLKQEEDEKD KRKLIESGDL PNLELHEDKF LLDLSKFKIY Y DNNHGYEW WDTAYLDEKG ELMEKYDMNG TSPAEEKLAE DIDEVDDDDD DEHPSIRYVA HPLPEKINEA KVSIKAYLTQ HE RKRLRRN RRKMAREARE IKIKLGLLPK PEPKVKLSNM MSVFENDQNI TDPTAWEKVV KDQVDLRKRK HLEENERRHE DAI KRRKEA VNMNVEKPTV YHCKVFQFKN LQNPKIRFKL KMNSKELSLK GLCLRIRDDG PGIIIVVGNE KSCKFYENLV MKRI KWNED FELHTNTGDI KMDMHNNSIS KTWEGYLQDC KFKGWFMKVC NDQDSLLRTL GQFDSEHFYS PVQT

Macromolecule #15: U4/U6 small nuclear ribonucleoprotein PRP4

• Macromolecule: Name: U4/U6 small nuclear ribonucleoprotein PRP4 / type: protein_or_peptide / ID: 15 / Details: U4/U6 small nuclear ribonucleoprotein PRP4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 52.506984 KDa

Sequence

String:

MSKYIALENL PVDLQHKGAT QNESTADILK QLPHERLQAV LEKIPEEDLE VRRLLSILKK PEVVENEDVQ QRRIRLAEIL MVDEIDLEN INNMENINGE EVDEEDDEDF FTPATSELIF ARRFLINYSL ERSRKRLQKE MERHQKFNTR QELLSRRTEL Q RMANLELA GSQLVSTKPI SAVSLSTDDM VVATGSWAGD LQVLNSQTLQ PLTQKLDSHV GKIGAIDWHP DSNNQMISCA ED GLIKNFQ YSNEEGGLRL LGDLVGHERR ISDVKYHPSG KFIGSASHDM TWRLWDASTH QELLLQEGHD KGVFSLSFQC DGS LVCSGG MDSLSMLWDI RSGSKVMTLA GHSKPIYTVA WSPNGYQVAT GGGDGIINVW DIRKRDEGQL NQILAHRNIV TQVR FSKED GGKKLVSCGY DNLINVYSSD TWLKMGSLAG HTDKIISLDI SNNSHFLVSG GWDRSIKLWN

Macromolecule #17: Pre-mRNA-splicing factor 6

• Macromolecule: Name: Pre-mRNA-splicing factor 6 / type: protein_or_peptide / ID: 17 / Details: Pre-mRNA-splicing factor 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 104.370133 KDa

Sequence

String:

MERPSFLDQE PPAGYVPGIG RGATGFSTKE KQVVSNDDKG RRIPKRYREN LNNHLQSQPK DDEDDEAANV FKTLELKLAQ KKKKRANEK DDDNSVDSSN VKRQFADLKE SLAAVTESEW MDIPDATDFT RRNKRNRIQE QLNRKTYAAP DSLIPGNVDL N KLTEEREK LLQSQIDENL AQLTKNASNP IQVNKPNAAT DALSYLKDLE NDRVNSLSDA TLEDLQKMRT ILKSYRKADP TN PQGWIAS ARLEEKARKF SVAKKIIENG CQECPRSSDI WLENIRLHES DVHYCKTLVA TAINFNPTSP LLWFKAIDLE STT VNKYRV VRKALQEIPR DEGLWKLAVS FEADKAQVIK MLEKATQFIP QSMDLLTAYT NLQSYHNAKM TLNSFRKILP QEPE IWIIS TLLEERNNPD IPVDKLVSLL KEGLLELSKN GYKATLSAWL KRAEALNDAP NSNLTCQAIV YAILEWLRES GEYES ELNN VDQILEKMPH SKVQIAVLKK LIQWDPCDTV LWSRLKMATE SYHKIEELLA FFQELLFQTK NSDDIRANMR EKSPGL LMM YVSEYWKAQK GDTRQTLVLI DQIIDFAPHN LDLRFFKIKL LGRSLQLDEL RDFFQQTFSS LEDFKISGTE RLYYKYV NF LRYQDLNEEA IKFLNERCLK SFPICHKFFL QLGQIYHSMG NIEMSRETYL SGTRLVPNCP LLWVSLSKID EIDLKNPV R ARSILDRGLL KNPDDVLFYI AKIQMEIRLG NLDQAELLVT QALQKFPSNA LLWVEQIKLF KHGNKSSLKK TIFQDALRR TQNDHRVLLE IGVSFYAEAQ YETSLKWLER ALKKCSRYGD TWVWLFRTYA RLGKDTVDLY NMFDQCEPTY GPEWIAASKN VKMQYCTPR EILLRLMNDK

Macromolecule #18: 13 kDa ribonucleoprotein-associated protein

• Macromolecule: Name: 13 kDa ribonucleoprotein-associated protein / type: protein_or_peptide / ID: 18 / Details: 13 kDa ribonucleoprotein-associated protein / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 13.582855 KDa

Sequence

String:

MSAPNPKAFP LADAALTQQI LDVVQQAANL RQLKKGANEA TKTLNRGISE FIIMAADCEP IEILLHLPLL CEDKNVPYVF VPSRVALGR ACGVSRPVIA ASITTNDASA IKTQIYAVKD KIETLLI

Macromolecule #19: 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component

• Macromolecule: Name: 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component; type: protein_or_peptide / ID: 19 ; Details: 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 22.717799 KDa

Sequence

String:

MSNFGRRTWD REEYAEQARS GYDDRSLKAT LTPIELQALK SKYTNYDHLI KGSLKDLNKR KLTANTESLS SFKRGKKFGF YCDICNLTF KDTLQYIDHL NHKVHAIKFE NLFDEPLIID IRDNDDVPQE EFELCYHNLI KDFVEVRSME TQSKRKRLLD T DVEKAKKV ATKPSIESES KVSQMMGFSN FATSKK

Macromolecule #20: Pre-mRNA-splicing factor 38

• Macromolecule: Name: Pre-mRNA-splicing factor 38 / type: protein_or_peptide / ID: 20 / Details: Pre-mRNA-splicing factor 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 27.995619 KDa

Sequence

String:

MAVNEFQVES NISPKQLNNQ SVSLVIPRLT RDKIHNSMYY KVNLSNESLR GNTMVELLKV MIGAFGTIKG QNGHLHMMVL GGIEFKCIL MKLIEIRPNF QQLNFLLNVK NENGFDSKYI IALLLVYARL QYYYLNGNNK NDDDENDLIK LFKVQLYKYS Q HYFKLKSF PLQVDCFAHS YNEELCIIHI DELVDWLATQ DHIWGIPLGK CQWNKIYNSD EESSSSESES NGDSEDDNDT SS ES

Macromolecule #21: Pre-mRNA-splicing factor SPP381

• Macromolecule: Name: Pre-mRNA-splicing factor SPP381 / type: protein_or_peptide / ID: 21 / Details: Pre-mRNA-splicing factor SPP381 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 33.830926 KDa

Sequence

String:

MSFRHFKRRL DTSSADESSS ADEEHPDQNV SLTEKSASLS HSDLGGEILN GTGKNRTPND GQESNESDGS PESDESPESE ESSDNSDSS DSDDMRPLPR PLFMKKKANN LQKATKIDQP WNAQDDARVL QTKKENMIKN IDKANQVAKN YETMKLRLNT N YSTNEELI KQCLLLDDND EVDSEKERQK WFERQNERKQ KHRRIQLAKQ RESEEYEAKR FEAMQKGKDG NTKYDVILDK EK EKLDHKK QRSAEKVEKS HNNNRYKITR TKNVEFGDLG KNSRDYEETE YSVI

Macromolecule #22: U2 snRNP component HSH155

• Macromolecule: Name: U2 snRNP component HSH155 / type: protein_or_peptide / ID: 22 / Details: U2 snRNP component HSH155 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 110.166672 KDa

Sequence

String:

MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR EDSYHKRRFD MKFEPDSDTQ TVTSSENTQ DAVVPRKRKS RWDVKGYEPP DESSTAVKEN SDSALVNVEG IHDLMFFKPS DHKYFADVIS KKPIDELNKD E KKERTLSM LLLKIKNGNT ASRRTSMRIL TDKAVTFGPE MIFNRLLPIL LDRSLEDQER HLMIKTIDRV LYQLGDLTKP YV HKILVVA APLLIDEDPM VRSTGQEIIT NLSTVAGLKT ILTVMRPDIE NEDEYVRNVT SRAAAVVAKA LGVNQLLPFI NAA CHSRKS WKARHTGIKI VQQIGILLGI GVLNHLTGLM SCIKDCLMDD HVPVRIVTAH TLSTLAENSY PYGIEVFNVV LEPL WKGIR SHRGKVLSSF LKAVGSMIPL MDPEYAGYYT TEAMRIIRRE FDSPDDEMKK TILLVLQKCS AVESITPKFL REEIA PEFF QKFWVRRVAL DRPLNKVVTY TTVTLAKKLG CSYTIDKLLT PLRDEAEPFR TMAVHAVTRT VNLLGTADLD ERLETR LID ALLIAFQEQT NSDSIIFKGF GAVTVSLDIR MKPFLAPIVS TILNHLKHKT PLVRQHAADL CAILIPVIKN CHEFEML NK LNIILYESLG EVYPEVLGSI INAMYCITSV MDLDKLQPPI NQILPTLTPI LRNKHRKVEV NTIKFVGLIG KLAPTYAP P KEWMRICFEL LELLKSTNKE IRRSANATFG FIAEAIGPHD VLVALLNNLK VQERQLRVCT AVAIGIVAKV CGPYNVLPV IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP LLEDALTDRD LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIF ETSPHAIMRI LEGLEALSQA LGPGLFMNYI WAGLFHPAKN VRKAFWRVYN NMYVMYQDAM VPFYPVTPDN N EEYIEELD LVL

Macromolecule #23: Pre-mRNA-splicing factor RSE1

• Macromolecule: Name: Pre-mRNA-splicing factor RSE1 / type: protein_or_peptide / ID: 23 / Details: Pre-mRNA-splicing factor RSE1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 153.956781 KDa

Sequence

String:

MWGGGKMAVV SLSPHTAKMR KLFGQASTTM AYDGLKREAE RRTRSDHNIT MVAKDDELYL YHLTLKKQTN FVHSCIGHFV DLEAGSKRE QSQLCVATET HLELYDTADG ELKLIAKFQN LFATITSMKS LDLPHSGSRA KASNWPTFLA LTSDSGNLSI V QIIMHAGA LRLKTLVNQP LTRTTLRRVS PISYMEIDPN GRCIILSSVE QNKLCFLVDY AQKLRISSPL EIIRPHMVTL DM AVVDVNF NNPCFVTLEI DNAATQLSVH LIFYVLELGL NHIVKKADYL VNPSANFVLS LPDLSRYNIT TSLSDNNYDA DYD TLFNPF VVIGFENHIL VKDMNGFFSL KVEIPKRSIT NSRHKNVTII SGIVQKLKND FFVLLQSNHG DLFKLTVSPD TNDR NRPLV QLSYFDTIQN SHQLHIFKNG YLFALSEMNN NFLFQFEKLG VEKNDFSNVL TSKDPNKSLV FEPSIKLQNL SILSQ QLNL NPSIKSQIVS DSPLSIATKH FTNNKIITLT NAVNYSNLIS TSLPPNATKL WLIPDPATTG DNNTLLFITF PKKTMI LQI DNESMEELTP DEATRSAFKL SQDTTIHTCL MGSHSIIQVC TAELRHIVPT GKSRYSNKLT WVPPAGIRIV CATSSKT QL IISLSNYELV YFKIDVSSDS LIELTTHPEL DTMPSKVAIV QDTQHADLLA IADNEGMIKI MSLKDQKEDF LTVISLQL V SEKISDMIMV RDSSIGQLNL HVGLENGVYM KFHIGDVDGS FTDIKRRFLG LKPVSLSYLR EISVSLNNEE EEEEEEDDD DEKEEEEINS SGAKWMSCVV CHSSSTWVSY TWKNVWTIRQ LKDQNMLSCS KFVNADVAIN GVCSISSSGR LNIGRVSNFP TLDNWFHVH ESSVNKQENG GGDESNEEEE DEMEEEMEML QISTFRPRTI LSFPNNPKSI LFIDNHSGKK QCRISLQIDG E CLKFGSSD HLYKILDDID CVSAAIIDFT RQADHLIICA GDKRLLTYKI LVNKDKLSFD IELLHQTEII SPIHAMLKFK NF LLTAMGS TIVLYGLGKK QLLRRSVTQT PVSITKIVSM HQWNYERLAV GDIHESVTLF IWDPAGNVFI PYVDDSVKRH VTV LKFLDE ATVIGADRYG NAWTLRSPPE CEKIMSNHDP SELSNGAIKY PLDVITLQQK LPNTYDCKFK FQLLNHFFVN DIIT DFHIL DSLSNSDRPG CIYMGLQGTV GCFIPLLSKG NVFMMGNIEN IMAEADDTFY LDYESRKKNN NMRKEDDEEE SGSVV LQGR HGIEDEIICE GSCSILGRDH QEYRSYYAPV RKVIDGDLCE NFLRLSLNEQ EFLAKNLKSV QVEDIIQTIN EVRTNY M

Macromolecule #24: Cold sensitive U2 snRNA suppressor 1

• Macromolecule: Name: Cold sensitive U2 snRNA suppressor 1 / type: protein_or_peptide / ID: 24 / Details: Cold sensitive U2 snRNA suppressor 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 50.210699 KDa

Sequence

String:

MARTKSRKRS GNNQNKNASV VNNKAEIAAM IDARRLEQKK KGGVTNSKGK TNKVVDAKLE KEFKDVLQRF QVQENDTPKE ITKDEKNNH VVIVEKNPVM NRKHTAEDEL EDTPSDGIEE HLSARKRRKT EKPSLSQLKS QVPYPQIIEW YDCDARYPGL L ASIKCTKN VIPVPSHWQS KKEYLSGRSL LGKRPFELPD IIKKTNIEQM RSTLPQSGLD GQDEKSLKEA SRARVQPKMG AL DLDYKKL HDVFFKIGAN WKPDHLLCFG DVYYENRNLF EETNWKRMVD HKRPGRISQE LRAIMNLPEG QLPPWCMKMK DIG LPTGYP DLKIAGLNWD ITNLKGDVYG KIIPNHHSRS KKQGRNYFGA LISFETPEFE NSKEDTQANA ENGRQDDKID DEVE HKLDH FQEDISEVTS AEEKLERNEE ESEKQLYTVL

Macromolecule #25: Protein HSH49

• Macromolecule: Name: Protein HSH49 / type: protein_or_peptide / ID: 25 / Details: Protein HSH49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 24.534152 KDa

Sequence

String:

MNYSADSGNT VYVGNIDPRI TKEQLYELFI QINPVLRIKY PKDKVLQAYQ GYAFIEFYNQ GDAQYAIKIM NNTVRLYDRL IKVRQVTNS TGTTNLPSNI SKDMILPIAK LFIKNLADSI DSDQLVKIFN KFGKLIREPE IFYLSNGKLK CAYVYFEDFE K ADLAIKSL NNQLVANNRI TVDYAFKENG KGNAKYGDDV DRLLNKEALK HNMLK

Macromolecule #26: Pre-mRNA-splicing factor RDS3

• Macromolecule: Name: Pre-mRNA-splicing factor RDS3 / type: protein_or_peptide / ID: 26 / Details: Pre-mRNA-splicing factor RDS3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 12.283573 KDa

Sequence

String:

MSRHQFDLIM CLKQPGVQTG LLCEKCDGKC PICDSYVRPK RKVRVCENCS FGKQAKNCII CNLNVGVNDA FYCWECCRLG KDKDGCPRI LNLGSNRLDR HFEKKKKV

Macromolecule #27: Pre-mRNA-splicing factor PRP9

• Macromolecule: Name: Pre-mRNA-splicing factor PRP9 / type: protein_or_peptide / ID: 27 / Details: Pre-mRNA-splicing factor PRP9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 63.126445 KDa

Sequence

String:

MNLLETRRSL LEEMEIIENA IAERIQRNPE LYYHYIQESS KVFPDTKLPR SSLIAENKIY KFKKVKRKRK QIILQQHEIN IFLRDYQEK QQTFNKINRP EETQEDDKDL PNFERKLQQL EKELKNEDEN FELDINSKKD KYALFSSSSD PSRRTNILSD R ARDLDLNE IFTRDEQYGE YMELEQFHSL WLNVIKRGDC SLLQFLDILE LFLDDEKYLL TPPMDRKNDR YMAFLLKLSK YV ETFFFKS YALLDAAAVE NLIKSDFEHS YCRGSLRSEA KGIYCPFCSR WFKTSSVFES HLVGKIHKKN ESKRRNFVYS EYK LHRYLK YLNDEFSRTR SFVERKLAFT ANERMAEMDI LTQKYEAPAY DSTEKEGAEQ VDGEQRDGQL QEEHLSGKSF DMPL GPDGL PMPYWLYKLH GLDREYRCEI CSNKVYNGRR TFERHFNEER HIYHLRCLGI EPSSVFKGIT KIKEAQELWK NMQGQ SQLT SIAAVPPKPN PSQLKVPTEL ELEEEDEEGN VMSKKVYDEL KKQGLV

Macromolecule #28: Pre-mRNA-splicing factor PRP11

• Macromolecule: Name: Pre-mRNA-splicing factor PRP11 / type: protein_or_peptide / ID: 28 / Details: Pre-mRNA-splicing factor PRP11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 29.962809 KDa

Sequence

String:

MNYLEGVGSK KGGGGIASES QFNLQRRKEV ESLLSKGENV PYTFQDEKDD QVRSNPYIYK NHSGKLVCKL CNTMHMSWSS VERHLGGKK HGLNVLRRGI SIEKSSLGRE GQTTHDFRQQ QKIIEAKQSL KNNGTIPVCK IATVKNPKNG SVGLAIQVNY S SEVKENSV DSDDKAKVPP LIRIVSGLEL SDTKQKGKKF LVIAYEPFEN IAIELPPNEI LFSENNDMDN NNDGVDELNK KC TFWDAIS KLYYVQFFFK QAEQEQADV

Macromolecule #29: Pre-mRNA-splicing factor PRP21

• Macromolecule: Name: Pre-mRNA-splicing factor PRP21 / type: protein_or_peptide / ID: 29 / Details: Pre-mRNA-splicing factor PRP21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 33.111512 KDa

Sequence

String:

MEPEDTQLKE DIKTTVNYIK QHGVEFENKL LEDERFSFIK KDDPLHEYYT KLMNEPTDTV SGEDNDRKSE REIARPPDFL FSQYDTGIS RRDMEVIKLT ARYYAKDKSI VEQMISKDGE ARLNFMNSSH PLHKTFTDFV AQYKRVYSFT GQEIKKSKRT I LDNCFERT QYWEFEKDKD REHDKLVELC KIQFAAIPWD KFTQVAKFSI PEDTEIFEGS LDLEQMRLRR VQTGIKLFDS IK PTNEEEK IVSDQGKQKG GDSKGKKRKI RAVGETRLKK SKK

Macromolecule #30: U2 small nuclear ribonucleoprotein A'

• Macromolecule: Name: U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 30 / Details: U2 small nuclear ribonucleoprotein A' / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 27.232252 KDa

Sequence

String:

MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL TKPTHILDLT NNDLIMIPDL SRRDDIHTLL LGRNNIVEV DGRLLPMNVQ NLTLSNNSIR RFEDLQRLRR APRTLKNLTL IGNQVCHLAN YREHVLRLVP HLETLDFQNV T AEERKSAM SFPRQADGDT LGPVNTAIRD NGSRDKTMEI MNLVVSKMTV ERRNELKKQL AEATSLEEIA RLEKLLSGGV

Macromolecule #31: Unknown

• Macromolecule: Name: Unknown / type: protein_or_peptide / ID: 31 / Details: Unknown protein helices within B complex / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Saccharomyces cerevisiae (brewer's yeast)
• Molecular weight: Theoretical: 7.907637 KDa

Sequence

String:

AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA

Macromolecule #32: U2 small nuclear ribonucleoprotein B''

• Macromolecule: Name: U2 small nuclear ribonucleoprotein B'' / type: protein_or_peptide / ID: 32 / Details: U2 small nuclear ribonucleoprotein B'' / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 12.850944 KDa

Sequence

String:

MVEPARKKQR IDRDTHHTVA EPVTEAKNTL YVSQLNEKIN MQRLRVNLFL LFATFGEVLK VSMNFKKQRG QAFITMRTID QASLAQISL NGERFFGKPL KVEFSKSETK TL

Macromolecule #33: RDS3 complex subunit 10

• Macromolecule: Name: RDS3 complex subunit 10 / type: protein_or_peptide / ID: 33 / Details: RDS3 complex subunit 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 10.045401 KDa

Sequence

String:

MAEKQRQLKL QKIYKQKYIG LGDESTTREQ WQRNVRNDTL NTLQGHSASL EYVSLSRGDL SIRDTRIHLL KSMSPGYKAY LREER

Macromolecule #34: U6 snRNA-associated Sm-like protein LSm2

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm2 / type: protein_or_peptide / ID: 34 / Details: U6 snRNA-associated Sm-like protein LSm2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 11.177888 KDa

Sequence

String:

MLFFSFFKTL VDQEVVVELK NDIEIKGTLQ SVDQFLNLKL DNISCTDEKK YPHLGSVRNI FIRGSTVRYV YLNKNMVDTN LLQDATRRE VMTERK

Macromolecule #35: Small nuclear ribonucleoprotein-associated protein B

• Macromolecule: Name: Small nuclear ribonucleoprotein-associated protein B / type: protein_or_peptide / ID: 35 ; Details: Small nuclear ribonucleoprotein-associated protein B; Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 22.42699 KDa

Sequence

String:

MSKIQVAHSS RLANLIDYKL RVLTQDGRVY IGQLMAFDKH MNLVLNECIE ERVPKTQLDK LRPRKDSKDG TTLNIKVEKR VLGLTILRG EQILSTVVED KPLLSKKERL VRDKKEKKQA QKQTKLRKEK EKKPGKIAKP NTANAKHTSS NSREIAQPSS S RYNGGNDN IGANRSRFNN EAPPQTRKFQ PPPGFKRK

Macromolecule #36: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 36 / Details: Small nuclear ribonucleoprotein Sm D3 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 11.240139 KDa

Sequence

String:

MTMNGIPVKL LNEAQGHIVS LELTTGATYR GKLVESEDSM NVQLRDVIAT EPQGAVTHMD QIFVRGSQIK FIVVPDLLKN APLFKKNSS RPMPPIRGPK RR

Macromolecule #37: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 37 / Details: Small nuclear ribonucleoprotein E / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 10.385098 KDa

Sequence

String:

MSNKVKTKAM VPPINCIFNF LQQQTPVTIW LFEQIGIRIK GKIVGFDEFM NVVIDEAVEI PVNSADGKED VEKGTPLGKI LLKGDNITL ITSAD

Macromolecule #38: Small nuclear ribonucleoprotein F

• Macromolecule: Name: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 38 / Details: Small nuclear ribonucleoprotein F / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 9.669945 KDa

Sequence

String:

MSESSDISAM QPVNPKPFLK GLVNHRVGVK LKFNSTEYRG TLVSTDNYFN LQLNEAEEFV AGVSHGTLGE IFIRCNNVLY IRELPN

Macromolecule #39: Small nuclear ribonucleoprotein G

• Macromolecule: Name: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 39 / Details: Small nuclear ribonucleoprotein G / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 8.490809 KDa

Sequence

String:

MVSTPELKKY MDKKILLNIN GSRKVAGILR GYDIFLNVVL DDAMEINGED PANNHQLGLQ TVIRGNSIIS LEALDAI

Macromolecule #40: Small nuclear ribonucleoprotein Sm D1

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 40 / Details: Small nuclear ribonucleoprotein Sm D1 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 16.296798 KDa

Sequence

String:

MKLVNFLKKL RNEQVTIELK NGTTVWGTLQ SVSPQMNAIL TDVKLTLPQP RLNKLNSNGI AMASLYLTGG QQPTASDNIA SLQYINIRG NTIRQIILPD SLNLDSLLVD QKQLNSLRRS GQIANDPSKK RRRDFGAPAN KRPRRGL

Macromolecule #41: Small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 41 / Details: Small nuclear ribonucleoprotein Sm D2 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 12.876066 KDa

Sequence

String:

MSSQIIDRPK HELSRAELEE LEEFEFKHGP MSLINDAMVT RTPVIISLRN NHKIIARVKA FDRHCNMVLE NVKELWTEKK GKNVINRER FISKLFLRGD SVIVVLKTPV E

Macromolecule #42: U6 snRNA-associated Sm-like protein LSm4

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm4 / type: protein_or_peptide / ID: 42 / Details: U6 snRNA-associated Sm-like protein LSm4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 21.29807 KDa

Sequence

String:

MLPLYLLTNA KGQQMQIELK NGEIIQGILT NVDNWMNLTL SNVTEYSEES AINSEDNAES SKAVKLNEIY IRGTFIKFIK LQDNIIDKV KQQINSNNNS NSNGPGHKRY YNNRDSNNNR GNYNRRNNNN GNSNRRPYSQ NRQYNNSNSS NINNSINSIN S NNQNMNNG LGGSVQHHFN SSSPQKVEF

Macromolecule #43: U6 snRNA-associated Sm-like protein LSm5

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm5 / type: protein_or_peptide / ID: 43 / Details: U6 snRNA-associated Sm-like protein LSm5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 10.432954 KDa

Sequence

String:

MSLPEILPLE VIDKTINQKV LIVLQSNREF EGTLVGFDDF VNVILEDAVE WLIDPEDESR NEKVMQHHGR MLLSGNNIAI LVPGGKKTP TEAL

Macromolecule #44: U6 snRNA-associated Sm-like protein LSm6

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm6 / type: protein_or_peptide / ID: 44 / Details: U6 snRNA-associated Sm-like protein LSm6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Baker's yeast (brewer's yeast)
• Molecular weight: Theoretical: 9.406579 KDa

Sequence

String:

MSGKASTEGS VTTEFLSDII GKTVNVKLAS GLLYSGRLES IDGFMNVALS SATEHYESNN NKLLNKFNSD VFLRGTQVMY ISEQKI

Macromolecule #45: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 45 / Number of copies: 1 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #46: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 46 / Number of copies: 7 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 1.5 mg/mL

Buffer

pH: 7.9
Component:

Concentration	Formula
20.0 mM	HEPES
50.0 mM	KCl
0.2 mM	EDTA
1.0 mM	DTT
2.5 mM	Desthiobiotin

Details: Buffer pH: HEPES, 7.9; EDTA, 8.0

• Grid: Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. / Pretreatment - Atmosphere: AIR
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK III; Details: Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity..

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Specialist optics: Energy filter - Name: GIF Quantum
• Image recording: Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Dimensions - Width: 3710 pixel / Digitization - Dimensions - Height: 3838 pixel / Digitization - Frames/image: 1-20 / Number real images: 5115 / Average exposure time: 16.0 sec. / Average electron dose: 56.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Calibrated defocus max: 5.3 µm / Calibrated defocus min: 0.35000000000000003 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal magnification: 81000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Details: Movies were binned once and aligned using MOTIONCORR.
• Particle selection: Number selected: 496581
• Startup model: Type of model: OTHER; Details: The negative stain map of human B-delta-U1 (EMD-1066) was used as an initial reference for three-dimensional refinement of a 12229 particle negative stain data set of the yeast B complex. A single round of 3D classification revealed a B complex density from 3707 particles that was refined to an estimated resolution of 50 A. This density was low-pass filtered to 60 A and used for processing of cryo-EM data.
• Final reconstruction: Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 7.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.0); Details: A temperature factor of -170 A2 was applied (sharpened B2 map).; Number images used: 9559
• Initial angle assignment: Type: PROJECTION MATCHING / Software - Name: RELION (ver. 2.0)
• Final angle assignment: Type: PROJECTION MATCHING / Software - Name: RELION (ver. 2.0)
• Final 3D classification: Software - Name: RELION (ver. 2.0)

Atomic model buiding 1

• Refinement: Space: REAL / Overall B value: 170

Output model

PDB-5nrl:
Structure of a pre-catalytic spliceosome.