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- EMDB-3683: Structure of a pre-catalytic spliceosome (B2 map) -

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Basic information

Entry
Database: EMDB / ID: EMD-3683
TitleStructure of a pre-catalytic spliceosome (B2 map)
Map dataunsharpened B2 map
Sample
  • Complex: Pre-catalytic B complex Spliceosome
    • RNA: x 5 types
    • Protein or peptide: x 39 types
  • Ligand: x 2 types
Function / homology
Function and homology information


Sm-like protein family complex / maturation of 5S rRNA / spliceosomal conformational changes to generate catalytic conformation / mRNA decay by 5' to 3' exoribonuclease / snoRNA splicing / Lsm1-7-Pat1 complex / U6 snRNP / snoRNA guided rRNA 2'-O-methylation / positive regulation of RNA binding / box C/D sno(s)RNA 3'-end processing ...Sm-like protein family complex / maturation of 5S rRNA / spliceosomal conformational changes to generate catalytic conformation / mRNA decay by 5' to 3' exoribonuclease / snoRNA splicing / Lsm1-7-Pat1 complex / U6 snRNP / snoRNA guided rRNA 2'-O-methylation / positive regulation of RNA binding / box C/D sno(s)RNA 3'-end processing / generation of catalytic spliceosome for first transesterification step / deadenylation-dependent decapping of nuclear-transcribed mRNA / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / box C/D methylation guide snoRNP complex / splicing factor binding / U4/U6 snRNP / P-body assembly / 7-methylguanosine cap hypermethylation / pICln-Sm protein complex / spliceosomal tri-snRNP complex / small nuclear ribonucleoprotein complex / sno(s)RNA-containing ribonucleoprotein complex / U4 snRNA binding / SMN-Sm protein complex / mRNA cis splicing, via spliceosome / U2-type spliceosomal complex / U2-type prespliceosome assembly / commitment complex / U2-type catalytic step 2 spliceosome / U4 snRNP / U2 snRNP / poly(U) RNA binding / nuclear-transcribed mRNA catabolic process / U1 snRNP / U3 snoRNA binding / U2-type prespliceosome / tRNA processing / precatalytic spliceosome / spliceosomal complex assembly / generation of catalytic spliceosome for second transesterification step / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA 3'-splice site recognition / mRNA 5'-splice site recognition / spliceosomal tri-snRNP complex assembly / U5 snRNA binding / U5 snRNP / U2 snRNA binding / spliceosomal snRNP assembly / U6 snRNA binding / pre-mRNA intronic binding / maturation of SSU-rRNA / U1 snRNA binding / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / U4/U6 x U5 tri-snRNP complex / catalytic step 2 spliceosome / small-subunit processome / P-body / spliceosomal complex / mRNA splicing, via spliceosome / rRNA processing / metallopeptidase activity / cytosolic large ribosomal subunit / RNA helicase activity / nucleic acid binding / RNA helicase / response to xenobiotic stimulus / mRNA binding / GTPase activity / GTP binding / nucleolus / ATP hydrolysis activity / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / ATP binding / identical protein binding / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Pre-mRNA-splicing factor Prp9, N-terminal / Pre-mRNA-splicing factor PRP9 N-terminus / U4/U6.U5 small nuclear ribonucleoprotein component Snu23 / Pre-mRNA-splicing factor 38 / PRP38 family / SNU66/SART1 family / HIND motif / SART-1 family / HIND motif / Pre-mRNA processing factor 4 (PRP4)-like ...Pre-mRNA-splicing factor Prp9, N-terminal / Pre-mRNA-splicing factor PRP9 N-terminus / U4/U6.U5 small nuclear ribonucleoprotein component Snu23 / Pre-mRNA-splicing factor 38 / PRP38 family / SNU66/SART1 family / HIND motif / SART-1 family / HIND motif / Pre-mRNA processing factor 4 (PRP4)-like / Splicing Factor Motif, present in Prp18 and Pr04 / Prp31 C-terminal / U4/U6 small nuclear ribonucleoprotein Prp31 / Prp31 C terminal domain / Pre-mRNA-splicing factor 3 / U4/U6 small nuclear ribonucleoprotein Prp3 / Sm-like protein Lsm8 / pre-mRNA processing factor 3 domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / U6 snRNA-associated Sm-like protein Lsm1/8 / Dim1 family / U6 snRNA-associated Sm-like protein LSm2 / Sm-like protein Lsm4 / Mitosis protein DIM1 / Mitosis protein DIM1 / Sm-like protein Lsm7 / PRP1 splicing factor, N-terminal / Sm-like protein LSm5 / Sm-like protein Lsm3 / U6 snRNA-associated Sm-like protein Lsm3 / PRP1 splicing factor, N-terminal / Splicing factor 3A subunit 1 / Splicing factor 3A subunit 1, conserved domain / Pre-mRNA splicing factor PRP21 like protein / SF3A2 domain / Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11) / Splicing factor SF3a60 /Prp9 subunit, C-terminal / SF3A3 domain / SF3B4, RNA recognition motif 1 / SF3a60/Prp9 C-terminal / Pre-mRNA-splicing factor SF3A3, of SF3a complex, Prp9 / SWAP/Surp / SWAP/Surp superfamily / Surp module / SURP motif repeat profile. / Suppressor-of-White-APricot splicing regulator / Domain of unknown function DUF382 / Domain of unknown function (DUF382) / RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) / Zinc-finger of C2H2 type / PHF5-like / PHF5-like protein / PSP, proline-rich / PSP / proline-rich domain in spliceosome associated proteins / Splicing factor 3B subunit 5/RDS3 complex subunit 10 / Splicing factor 3B subunit 10 (SF3b10) / Matrin/U1-C, C2H2-type zinc finger / Splicing factor 3B subunit 1-like / Zinc finger matrin-type profile. / U2A'/phosphoprotein 32 family A, C-terminal / occurring C-terminal to leucine-rich repeats / Leucine-rich repeat / Pre-mRNA-splicing factor Syf1-like / NOSIC / Brr2, N-terminal helicase PWI domain / : / N-terminal helicase PWI domain / Pre-mRNA-splicing helicase BRR2 plug domain / NOSIC (NUC001) domain / Nop domain / Nop domain superfamily / Nop, C-terminal domain / snoRNA binding domain, fibrillarin / Nop domain profile. / H/ACA ribonucleoprotein complex, subunit Nhp2-like / Snu114, GTP-binding domain / Zinc-finger double-stranded RNA-binding / Zinc finger, double-stranded RNA binding / Sec63 Brl domain / 116kDa U5 small nuclear ribonucleoprotein component, N-terminal / 116kDa U5 small nuclear ribonucleoprotein component, C-terminal / 116 kDa U5 small nuclear ribonucleoprotein component N-terminus / Small nuclear ribonucleoprotein Sm D3 / Sec63 domain / Sec63 Brl domain / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein G / Small nuclear ribonucleoprotein F / Sm-like protein Lsm7/SmG / Like-Sm (LSM) domain containing protein, LSm4/SmD1/SmD3 / Matrin/U1-C-like, C2H2-type zinc finger / U1-like zinc finger / Sm-like protein Lsm6/SmF / LSM domain / LSM domain, eukaryotic/archaea-type / snRNP Sm proteins / HAT (Half-A-TPR) repeat
Similarity search - Domain/homology
U6 snRNA-associated Sm-like protein LSm6 / RDS3 complex subunit 10 / Pre-mRNA-splicing factor 6 / Pre-mRNA-splicing factor PRP9 / U4/U6 small nuclear ribonucleoprotein PRP4 / Pre-mRNA-splicing factor PRP21 / Pre-mRNA-splicing helicase BRR2 / Pre-mRNA-splicing factor 8 / Pre-mRNA-splicing factor SNU114 / U6 snRNA-associated Sm-like protein LSm2 ...U6 snRNA-associated Sm-like protein LSm6 / RDS3 complex subunit 10 / Pre-mRNA-splicing factor 6 / Pre-mRNA-splicing factor PRP9 / U4/U6 small nuclear ribonucleoprotein PRP4 / Pre-mRNA-splicing factor PRP21 / Pre-mRNA-splicing helicase BRR2 / Pre-mRNA-splicing factor 8 / Pre-mRNA-splicing factor SNU114 / U6 snRNA-associated Sm-like protein LSm2 / Pre-mRNA-splicing factor SPP381 / 13 kDa ribonucleoprotein-associated protein / Small nuclear ribonucleoprotein-associated protein B / U6 snRNA-associated Sm-like protein LSm4 / U6 snRNA-associated Sm-like protein LSm5 / Small nuclear ribonucleoprotein G / U2 small nuclear ribonucleoprotein B'' / Small nuclear ribonucleoprotein Sm D3 / U6 snRNA-associated Sm-like protein LSm8 / Pre-mRNA-processing factor 31 / U2 snRNP component HSH155 / U6 snRNA-associated Sm-like protein LSm7 / Small nuclear ribonucleoprotein F / U6 snRNA-associated Sm-like protein LSm3 / Pre-mRNA-splicing factor 38 / Small nuclear ribonucleoprotein Sm D1 / Cold sensitive U2 snRNA suppressor 1 / U4/U6 small nuclear ribonucleoprotein PRP3 / Pre-mRNA-splicing factor RSE1 / Small nuclear ribonucleoprotein Sm D2 / Spliceosomal protein DIB1 / Pre-mRNA-splicing factor RDS3 / Pre-mRNA-splicing factor PRP11 / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein E / 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component / 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component / Protein HSH49
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast) / Baker's yeast (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 7.2 Å
AuthorsPlaschka C / Lin P-C / Nagai K
Funding support United Kingdom, 2 items
OrganizationGrant numberCountry
Medical Research Council (United Kingdom)MC_U105184330 United Kingdom
European Research Council693087 - SPLICE3D United Kingdom
CitationJournal: Nature / Year: 2017
Title: Structure of a pre-catalytic spliceosome.
Authors: Clemens Plaschka / Pei-Chun Lin / Kiyoshi Nagai /
Abstract: Intron removal requires assembly of the spliceosome on precursor mRNA (pre-mRNA) and extensive remodelling to form the spliceosome's catalytic centre. Here we report the cryo-electron microscopy ...Intron removal requires assembly of the spliceosome on precursor mRNA (pre-mRNA) and extensive remodelling to form the spliceosome's catalytic centre. Here we report the cryo-electron microscopy structure of the yeast Saccharomyces cerevisiae pre-catalytic B complex spliceosome at near-atomic resolution. The mobile U2 small nuclear ribonucleoprotein particle (snRNP) associates with U4/U6.U5 tri-snRNP through the U2/U6 helix II and an interface between U4/U6 di-snRNP and the U2 snRNP SF3b-containing domain, which also transiently contacts the helicase Brr2. The 3' region of the U2 snRNP is flexibly attached to the SF3b-containing domain and protrudes over the concave surface of tri-snRNP, where the U1 snRNP may reside before its release from the pre-mRNA 5' splice site. The U6 ACAGAGA sequence forms a hairpin that weakly tethers the 5' splice site. The B complex proteins Prp38, Snu23 and Spp381 bind the Prp8 N-terminal domain and stabilize U6 ACAGAGA stem-pre-mRNA and Brr2-U4 small nuclear RNA interactions. These results provide important insights into the events leading to active site formation.
History
DepositionApr 24, 2017-
Header (metadata) releaseMay 31, 2017-
Map releaseMay 31, 2017-
UpdateMay 24, 2023-
Current statusMay 24, 2023Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0258
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.0258
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5nrl
  • Surface level: 0.0258
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_3683.png

Downloads & links

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Map

FileDownload / File: emd_3683.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationunsharpened B2 map
Voxel sizeX=Y=Z: 1.43 Å
Density
Contour LevelBy AUTHOR: 0.0258 / Movie #1: 0.0258
Minimum - Maximum-0.01883009 - 0.080454715
Average (Standard dev.)-7.166218e-05 (±0.0032017468)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 686.39996 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.431.431.43
M x/y/z480480480
origin x/y/z0.0000.0000.000
length x/y/z686.400686.400686.400
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ450450450
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS480480480
D min/max/mean-0.0190.080-0.000

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Supplemental data

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Additional map: sharpened B2 map

Fileemd_3683_additional.map
Annotationsharpened B2 map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Pre-catalytic B complex Spliceosome

EntireName: Pre-catalytic B complex Spliceosome
Components
  • Complex: Pre-catalytic B complex Spliceosome
    • RNA: U2 snRNAU2 spliceosomal RNA
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm3
    • RNA: U4 snRNAU4 spliceosomal RNA
    • RNA: U5 snRNAU5 spliceosomal RNA
    • RNA: U6 snRNAU6 spliceosomal RNA
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm7
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm8
    • Protein or peptide: Pre-mRNA-splicing factor 8
    • Protein or peptide: Pre-mRNA-splicing helicase BRR2
    • Protein or peptide: Pre-mRNA-splicing factor SNU114
    • Protein or peptide: Spliceosomal protein DIB1Spliceosome
    • Protein or peptide: 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component
    • Protein or peptide: Pre-mRNA-processing factor 31
    • Protein or peptide: U4/U6 small nuclear ribonucleoprotein PRP3
    • Protein or peptide: U4/U6 small nuclear ribonucleoprotein PRP4
    • RNA: Yeast UBC4 gene for ubiquitin-conjugating enzyme
    • Protein or peptide: Pre-mRNA-splicing factor 6
    • Protein or peptide: 13 kDa ribonucleoprotein-associated protein
    • Protein or peptide: 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component
    • Protein or peptide: Pre-mRNA-splicing factor 38
    • Protein or peptide: Pre-mRNA-splicing factor SPP381
    • Protein or peptide: U2 snRNP component HSH155
    • Protein or peptide: Pre-mRNA-splicing factor RSE1
    • Protein or peptide: Cold sensitive U2 snRNA suppressor 1
    • Protein or peptide: Protein HSH49
    • Protein or peptide: Pre-mRNA-splicing factor RDS3
    • Protein or peptide: Pre-mRNA-splicing factor PRP9
    • Protein or peptide: Pre-mRNA-splicing factor PRP11
    • Protein or peptide: Pre-mRNA-splicing factor PRP21
    • Protein or peptide: U2 small nuclear ribonucleoprotein A'
    • Protein or peptide: Unknown
    • Protein or peptide: U2 small nuclear ribonucleoprotein B''
    • Protein or peptide: RDS3 complex subunit 10
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm2
    • Protein or peptide: Small nuclear ribonucleoprotein-associated protein B
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
    • Protein or peptide: Small nuclear ribonucleoprotein E
    • Protein or peptide: Small nuclear ribonucleoprotein F
    • Protein or peptide: Small nuclear ribonucleoprotein G
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
    • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm4
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm5
    • Protein or peptide: U6 snRNA-associated Sm-like protein LSm6
  • Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
  • Ligand: ZINC ION

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Supramolecule #1: Pre-catalytic B complex Spliceosome

SupramoleculeName: Pre-catalytic B complex Spliceosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast) / Strain: BCY123
Molecular weightTheoretical: 2.5 MDa

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Macromolecule #1: U2 snRNA

MacromoleculeName: U2 snRNA / type: rna / ID: 1 / Details: Saccharomyces cerevisiae U2 snRNA / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 376.267406 KDa
SequenceString: ACGAAUCUCU UUGCCUUUUG GCUUAGAUCA AGUG(PSU)AGUAU C(PSU)G(PSU)UCUUUU CAGUGUAACA ACUGAAAU G ACCUCAAUGA GGCUCAUUAC CUUUUAAUUU GUUACAAUAC ACAUUUUUUG GCACCCAAAA UAAUAAAAUG GACGGGAAG AGACUUUUUA ...String:
ACGAAUCUCU UUGCCUUUUG GCUUAGAUCA AGUG(PSU)AGUAU C(PSU)G(PSU)UCUUUU CAGUGUAACA ACUGAAAU G ACCUCAAUGA GGCUCAUUAC CUUUUAAUUU GUUACAAUAC ACAUUUUUUG GCACCCAAAA UAAUAAAAUG GACGGGAAG AGACUUUUUA AGCAAGUUGU UUUCCGCUAA UGUCAGGUCU CACUACUUUU UGCUGCUAUU UUUCUUCGCU CAUGGUUUCU UCAUAAGGC GUUUUUAUGA UGGUUUUUCG AAAUUGGUUU UUGAGACGAC GGUUGCUCAA GGUUAUUGUU UUUGUUUUCU U CUGGUUGU UUUCUAUUUU CUUUUUUUUA GCUUUCUGUU UCUCCCUUAG UUUGGCUUUU UGCUUCAUAC UCUUCCCUGU CU UUCCGAG CCGUUUAUGU CCAACGCGGG AUUUGGUUUU UCUUUAUCGA UGGGAAGAAA UGGUGCUAUA GUAGGUUGGG AGA UAAUAU UUAUGGUAUG GGGUGCUAGU GCGGAUGGGG CGCUCUUAUU GUUGAUUUCU UCGCUCGUCU UCUUUUUCUG GUGG CGCUG CAAGAGGAAG UUUUUCGACU UUGUUAUGAU UUUUGGUUUG CAAGGAAAGG UGUCUUACGA UUCUUUUUUU GAUGU AAUA GGAUAAGCUU GCUUAUCCCC CAAGUAUCGG CCAAAGUUGU UGAUUUUCCU UUUGAAGUGU CCUCGGUUUG AGGGGG UGU AGGGUGGGGU UGGUCUACAA UAAGAGUGUU CCAUUGUUAA CGUGCUGGCG UCUUUUACUA UAUUUUUUUU CCCAGUU UA UUUUGUGCUU AUUUUCUCAU UGAGGAGAAG GAGCUCUUCU CGCAGGAUAU AAAUGGAGGU UUGCUAAAGG GGAGGAGA U GUGUUUGUGA GAAUACUGCU GAGAGAGUUC UGGAAGAGAA AAAAAGGAGG CAAUGGAAGG CGUUUGCUGG GAAAAGAGA AGAGCCAUGA CUGCAUCUGU UGUUUCAAGG CCAGUUUUAU UAACCGCCUA UGUCAUAGAG GCGUUUUUUU UGGAGGGAUU UGAAGAAUG CCGGCGGCAU CAAGAAACGG ACUUGAUGGU UGACGCCUGU UUUUAAAGUU AGAGACGUCG CGACCCUCGC A CUUGUGGA GUCGUUCUUG ACUUUUACUU UGGUCGCUUG AUGUUUCUCU CGUCUUCCCG UUCGCUCUU

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Macromolecule #3: U4 snRNA

MacromoleculeName: U4 snRNA / type: rna / ID: 3 / Details: Saccharomyces cerevisiae U4 snRNA / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 51.186023 KDa
SequenceString:
AUCCUUAUGC ACGGGAAAUA CGCAUAUCAG UGAGGAUUCG UCCGAGAUUG UGUUUUUGCU GGUUGAAAUU UAAUUAUAAA CCAGACCGU CUCCUCAUGG UCAAUUCGGU GUUCGCUUUU GAAUACUUCA AGACUAUGUA GGGAAUUUUU GGAAUACCUU U

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Macromolecule #4: U5 snRNA

MacromoleculeName: U5 snRNA / type: rna / ID: 4 / Details: Saccharomyces cerevisiae U5 snRNA / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 68.643344 KDa
SequenceString: AAGCAGCUUU ACAGAUCAAU GGCGGAGGGA GGUCAACAUC AAGAACUGUG GGCCUUUUAU UGCCUAUAGA ACUUAUAACG AACAUGGUU CUUGCCUUUU ACCAGAACCA UCCGGGUGUU GUCUCCAUAG AAACAGGUAA AGCUGUCCGU UACUGUGGGC U UGCCAUAU ...String:
AAGCAGCUUU ACAGAUCAAU GGCGGAGGGA GGUCAACAUC AAGAACUGUG GGCCUUUUAU UGCCUAUAGA ACUUAUAACG AACAUGGUU CUUGCCUUUU ACCAGAACCA UCCGGGUGUU GUCUCCAUAG AAACAGGUAA AGCUGUCCGU UACUGUGGGC U UGCCAUAU UUUUUGGAAC UUUUCUGCCC UUUUUCUCAA UGAGUAAGGA GGGCGU

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Macromolecule #5: U6 snRNA

MacromoleculeName: U6 snRNA / type: rna / ID: 5 / Details: Saccharomyces cerevisiae U6 snRNA / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 35.883176 KDa
SequenceString:
GUUCGCGAAG UAACCCUUCG UGGACAUUUG GUCAAUUUGA AACAAUACAG AGAUGAUCAG CAGUUCCCCU GCAUAAGGAU GAACCGUUU UACAAAGAGA UUUAUUUCGU UUU

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Macromolecule #16: Yeast UBC4 gene for ubiquitin-conjugating enzyme

MacromoleculeName: Yeast UBC4 gene for ubiquitin-conjugating enzyme / type: rna / ID: 16
Details: Saccharomyces cerevisiae UBC4 pre-mRNA for ubiquitin-conjugating enzyme, in vitro transcribed.
Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 30.20073 KDa
SequenceString:
GUAUGUCUAA AGUUAUGGCC ACGUUUCAAA UGCGUGCUUU UUUUUUAAAA CUUAUGCUCU UAUUUACUAA CAAAAUCAAC AUGCUAUUG AACUAG

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Macromolecule #2: U6 snRNA-associated Sm-like protein LSm3

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm3 / type: protein_or_peptide / ID: 2 / Details: U6 snRNA-associated Sm-like protein LSm3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 10.039262 KDa
SequenceString:
METPLDLLKL NLDERVYIKL RGARTLVGTL QAFDSHCNIV LSDAVETIYQ LNNEELSESE RRCEMVFIRG DTVTLISTPS EDDDGAVEI

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Macromolecule #6: U6 snRNA-associated Sm-like protein LSm7

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm7 / type: protein_or_peptide / ID: 6 / Details: U6 snRNA-associated Sm-like protein LSm7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 13.027045 KDa
SequenceString:
MHQQHSKSEN KPQQQRKKFE GPKREAILDL AKYKDSKIRV KLMGGKLVIG VLKGYDQLMN LVLDDTVEYM SNPDDENNTE LISKNARKL GLTVIRGTIL VSLSSAEGSD VLYMQK

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Macromolecule #7: U6 snRNA-associated Sm-like protein LSm8

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm8 / type: protein_or_peptide / ID: 7 / Details: U6 snRNA-associated Sm-like protein LSm8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 12.403378 KDa
SequenceString:
MSATLKDYLN KRVVIIKVDG ECLIASLNGF DKNTNLFITN VFNRISKEFI CKAQLLRGSE IALVGLIDAE NDDSLAPIDE KKVPMLKDT KNKIENEHVI WEKVYESKTK

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Macromolecule #8: Pre-mRNA-splicing factor 8

MacromoleculeName: Pre-mRNA-splicing factor 8 / type: protein_or_peptide / ID: 8 / Details: Pre-mRNA-splicing factor 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 279.867469 KDa
SequenceString: MSGLPPPPPG FEEDSDLALP PPPPPPPGYE IEELDNPMVP SSVNEDTFLP PPPPPPSNFE INAEEIVDFT LPPPPPPPGL DELETKAEK KVELHGKRKL DIGKDTFVTR KSRKRAKKMT KKAKRSNLYT PKAEMPPEHL RKIINTHSDM ASKMYNTDKK A FLGALKYL ...String:
MSGLPPPPPG FEEDSDLALP PPPPPPPGYE IEELDNPMVP SSVNEDTFLP PPPPPPSNFE INAEEIVDFT LPPPPPPPGL DELETKAEK KVELHGKRKL DIGKDTFVTR KSRKRAKKMT KKAKRSNLYT PKAEMPPEHL RKIINTHSDM ASKMYNTDKK A FLGALKYL PHAILKLLEN MPHPWEQAKE VKVLYHTSGA ITFVNETPRV IEPVYTAQWS ATWIAMRREK RDRTHFKRMR FP PFDDDEP PLSYEQHIEN IEPLDPINLP LDSQDDEYVK DWLYDSRPLE EDSKKVNGTS YKKWSFDLPE MSNLYRLSTP LRD EVTDKN YYYLFDKKSF FNGKALNNAI PGGPKFEPLY PREEEEDYNE FNSIDRVIFR VPIRSEYKVA FPHLYNSRPR SVRI PWYNN PVSCIIQNDE EYDTPALFFD PSLNPIPHFI DNNSSLNVSN TKENGDFTLP EDFAPLLAEE EELILPNTKD AMSLY HSPF PFNRTKGKMV RAQDVALAKK WFLQHPDEEY PVKVKVSYQK LLKNYVLNEL HPTLPTNHNK TKLLKSLKNT KYFQQT TID WVEAGLQLCR QGHNMLNLLI HRKGLTYLHL DYNFNLKPTK TLTTKERKKS RLGNSFHLMR ELLKMMKLIV DTHVQFR LG NVDAFQLADG IHYILNHIGQ LTGIYRYKYK VMHQIRACKD LKHIIYYKFN KNLGKGPGCG FWQPAWRVWL NFLRGTIP L LERYIGNLIT RQFEGRSNEI VKTTTKQRLD AYYDLELRNS VMDDILEMMP ESIRQKKART ILQHLSEAWR CWKANIPWD VPGMPAPIKK IIERYIKSKA DAWVSAAHYN RERIKRGAHV EKTMVKKNLG RLTRLWIKNE QERQRQIQKN GPEITPEEAT TIFSVMVEW LESRSFSPIP FPPLTYKNDT KILVLALEDL KDVYASKVRL NASEREELAL IEEAYDNPHD TLNRIKKYLL T QRVFKPVD ITMMENYQNI SPVYSVDPLE KITDAYLDQY LWYEADQRKL FPNWIKPSDS EIPPLLVYKW TQGINNLSEI WD VSRGQSA VLLETTLGEM AEKIDFTLLN RLLRLIVDPN IADYITAKNN VVINFKDMSH VNKYGLIRGL KFASFIFQYY GLV IDLLLL GQERATDLAG PANNPNEFMQ FKSKEVEKAH PIRLYTRYLD RIYMLFHFEE DEGEELTDEY LAENPDPNFE NSIG YNNRK CWPKDSRMRL IRQDVNLGRA VFWEIQSRVP TSLTSIKWEN AFVSVYSKNN PNLLFSMCGF EVRILPRQRM EEVVS NDEG VWDLVDERTK QRTAKAYLKV SEEEIKKFDS RIRGILMASG STTFTKVAAK WNTSLISLFT YFREAIVATE PLLDIL VKG ETRIQNRVKL GLNSKMPTRF PPAVFYTPKE LGGLGMISAS HILIPASDLS WSKQTDTGIT HFRAGMTHED EKLIPTI FR YITTWENEFL DSQRVWAEYA TKRQEAIQQN RRLAFEELEG SWDRGIPRIS TLFQRDRHTL AYDRGHRIRR EFKQYSLE R NSPFWWTNSH HDGKLWNLNA YRTDVIQALG GIETILEHTL FKGTGFNSWE GLFWEKASGF EDSMQFKKLT HAQRTGLSQ IPNRRFTLWW SPTINRANVY VGFLVQLDLT GIFLHGKIPT LKISLIQIFR AHLWQKIHES IVFDICQILD GELDVLQIES VTKETVHPR KSYKMNSSAA DITMESVHEW EVSKPSLLHE TNDSFKGLIT NKMWFDVQLR YGDYDSHDIS RYVRAKFLDY T TDNVSMYP SPTGVMIGID LAYNMYDAYG NWFNGLKPLI QNSMRTIMKA NPALYVLRER IRKGLQIYQS SVQEPFLNSS NY AELFNND IKLFVDDTNV YRVTVHKTFE GNVATKAING CIFTLNPKTG HLFLKIIHTS VWAGQKRLSQ LAKWKTAEEV SAL VRSLPK EEQPKQIIVT RKAMLDPLEV HMLDFPNIAI RPTELRLPFS AAMSIDKLSD VVMKATEPQM VLFNIYDDWL DRIS SYTAF SRLTLLLRAL KTNEESAKMI LLSDPTITIK SYHLWPSFTD EQWITIESQM RDLILTEYGR KYNVNISALT QTEIK DIIL GQNIKAPSVK RQKMAELEAA RSEKQNDEEA AGASTVMKTK TINAQGEEIV VVASADYESQ TFSSKNEWRK SAIANT LLY LRLKNIYVSA DDFVEEQNVY VLPKNLLKKF IEISDVKIQV AAFIYGMSAK DHPKVKEIKT VVLVPQLGHV GSVQISN IP DIGDLPDTEG LELLGWIHTQ TEELKFMAAS EVATHSKLFA DKKRDCIDIS IFSTPGSVSL SAYNLTDEGY QWGEENKD I MNVLSEGFEP TFSTHAQLLL SDRITGNFII PSGNVWNYTF MGTAFNQEGD YNFKYGIPLE FYNEMHRPVH FLQFSELAG DEELEAEQID VFS

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Macromolecule #9: Pre-mRNA-splicing helicase BRR2

MacromoleculeName: Pre-mRNA-splicing helicase BRR2 / type: protein_or_peptide / ID: 9 / Details: Pre-mRNA-splicing helicase BRR2 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 246.470266 KDa
SequenceString: MTEHETKDKA KKIREIYRYD EMSNKVLKVD KRFMNTSQNP QRDAEISQPK SMSGRISAKD MGQGLCNNIN KGLKENDVAV EKTGKSASL KKIQQHNTIL NSSSDFRLHY YPKDPSNVET YEQILQWVTE VLGNDIPHDL IIGTADIFIR QLKENEENED G NIEERKEK ...String:
MTEHETKDKA KKIREIYRYD EMSNKVLKVD KRFMNTSQNP QRDAEISQPK SMSGRISAKD MGQGLCNNIN KGLKENDVAV EKTGKSASL KKIQQHNTIL NSSSDFRLHY YPKDPSNVET YEQILQWVTE VLGNDIPHDL IIGTADIFIR QLKENEENED G NIEERKEK IQHELGINID SLKFNELVKL MKNITDYETH PDNSNKQAVA ILADDEKSDE EEVTEMSNNA NVLGGEINDN ED DDEEYDY NDVEVNSKKK NKRALPNIEN DIIKLSDSKT SNIESVPIYS IDEFFLQRKL RSELGYKDTS VIQDLSEKIL NDI ETLEHN PVALEQKLVD LLKFENISLA EFILKNRSTI FWGIRLAKST ENEIPNLIEK MVAKGLNDLV EQYKFRETTH SKRE LDSGD DQPQSSEAKR TKFSNPAIPP VIDLEKIKFD ESSKLMTVTK VSLPEGSFKR VKPQYDEIHI PAPSKPVIDY ELKEI TSLP DWCQEAFPSS ETTSLNPIQS KVFHAAFEGD SNMLICAPTG SGKTNIALLT VLKALSHHYN PKTKKLNLSA FKIVYI APL KALVQEQVRE FQRRLAFLGI KVAELTGDSR LSRKQIDETQ VLVSTPEKWD ITTRNSNNLA IVELVRLLII DEIHLLH DD RGPVLESIVA RTFWASKYGQ EYPRIIGLSA TLPNYEDVGR FLRVPKEGLF YFDSSFRPCP LSQQFCGIKE RNSLKKLK A MNDACYEKVL ESINEGNQII VFVHSRKETS RTATWLKNKF AEENITHKLT KNDAGSKQIL KTEAANVLDP SLRKLIESG IGTHHAGLTR SDRSLSEDLF ADGLLQVLVC TATLAWGVNL PAHTVIIKGT DVYSPEKGSW EQLSPQDVLQ MLGRAGRPRY DTFGEGIII TDQSNVQYYL SVLNQQLPIE SQFVSKLVDN LNAEVVAGNI KCRNDAVNWL AYTYLYVRML ASPMLYKVPD I SSDGQLKK FRESLVHSAL CILKEQELVL YDAENDVIEA TDLGNIASSF YINHASMDVY NRELDEHTTQ IDLFRIFSMS EE FKYVSVR YEEKRELKQL LEKAPIPIRE DIDDPLAKVN VLLQSYFSQL KFEGFALNSD IVFIHQNAGR LLRAMFEICL KRG WGHPTR MLLNLCKSAT TKMWPTNCPL RQFKTCPVEV IKRLEASTVP WGDYLQLETP AEVGRAIRSE KYGKQVYDLL KRFP KMSVT CNAQPITRSV MRFNIEIIAD WIWDMNVHGS LEPFLLMLED TDGDSILYYD VLFITPDIVG HEFTLSFTYE LKQHN QNNL PPNFFLTLIS ENWWHSEFEI PVSFNGFKLP KKFPPPTPLL ENISISTSEL GNDDFSEVFE FKTFNKIQSQ VFESLY NSN DSVFVGSGKG TGKTAMAELA LLNHWRQNKG RAVYINPSGE KIDFLLSDWN KRFSHLAGGK IINKLGNDPS LNLKLLA KS HVLLATPVQF ELLSRRWRQR KNIQSLELMI YDDAHEISQG VYGAVYETLI SRMIFIATQL EKKIRFVCLS NCLANARD F GEWAGMTKSN IYNFSPSERI EPLEINIQSF KDVEHISFNF SMLQMAFEAS AAAAGNRNSS SVFLPSRKDC MEVASAFMK FSKAIEWDML NVEEEQIVPY IEKLTDGHLR APLKHGVGIL YKGMASNDER IVKRLYEYGA VSVLLISKDC SAFACKTDEV IILGTNLYD GAEHKYMPYT INELLEMVGL ASGNDSMAGK VLILTSHNMK AYYKKFLIEP LPTESYLQYI IHDTLNNEIA N SIIQSKQD CVDWFTYSYF YRRIHVNPSY YGVRDTSPHG ISVFLSNLVE TCLNDLVESS FIEIDDTEAE VTAEVNGGDD EA TEIISTL SNGLIASHYG VSFFTIQSFV SSLSNTSTLK NMLYVLSTAV EFESVPLRKG DRALLVKLSK RLPLRFPEHT SSG SVSFKV FLLLQAYFSR LELPVDFQND LKDILEKVVP LINVVVDILS ANGYLNATTA MDLAQMLIQG VWDVDNPLRQ IPHF NNKIL EKCKEINVET VYDIMALEDE ERDEILTLTD SQLAQVAAFV NNYPNVELTY SLNNSDSLIS GVKQKITIQL TRDVE PENL QVTSEKYPFD KLESWWLVLG EVSKKELYAI KKVTLNKETQ QYELEFDTPT SGKHNLTIWC VCDSYLDADK ELSFEI NVK

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Macromolecule #10: Pre-mRNA-splicing factor SNU114

MacromoleculeName: Pre-mRNA-splicing factor SNU114 / type: protein_or_peptide / ID: 10 / Details: Pre-mRNA-splicing factor SNU114 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 114.174008 KDa
SequenceString: MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTP LVEPVTERTK LQEHTIFTQL KKNIPKTRYN RDYMLSMANI PERIINVGVI GPLHSGKTSL MDLLVIDSHK R IPDMSKNV ...String:
MEGDDLFDEF GNLIGVDPFD SDEEESVLDE QEQYQTNTFE GSGNNNEIES RQLTSLGSKK ELGISLEHPY GKEVEVLMET KNTQSPQTP LVEPVTERTK LQEHTIFTQL KKNIPKTRYN RDYMLSMANI PERIINVGVI GPLHSGKTSL MDLLVIDSHK R IPDMSKNV ELGWKPLRYL DNLKQEIDRG LSIKLNGSTL LCTDLESKSR MINFLDAPGH VNFMDETAVA LAASDLVLIV ID VVEGVTF VVEQLIKQSI KNNVAMCFVI NKLDRLILDL KLPPMDAYLK LNHIIANINS FTKGNVFSPI DNNIIFASTK LGF TFTIKE FVSYYYAHSI PSSKIDDFTT RLWGSVYYHK GNFRTKPFEN VEKYPTFVEF ILIPLYKIFS YALSMEKDKL KNLL RSNFR VNLSQEALQY DPQPFLKHVL QLIFRQQTGL VDAITRCYQP FELFDNKTAH LSIPGKSTPE GTLWAHVLKT VDYGG AEWS LVRIYSGLLK RGDTVRILDT SQSESRQKRQ LHDISKTETS NEDEDEDDET PSCEVEEIGL LGGRYVYPVH EAHKGQ IVL IKGISSAYIK SATLYSVKSK EDMKQLKFFK PLDYITEAVF KIVLQPLLPR ELPKLLDALN KISKYYPGVI IKVEESG EH VILGNGELYM DCLLYDLRAS YAKIEIKISD PLTVFSESCS NESFASIPVS NSISRLGEEN LPGLSISVAA EPMDSKMI Q DLSRNTLGKG QNCLDIDGIM DNPRKLSKIL RTEYGWDSLA SRNVWSFYNG NVLINDTLPD EISPELLSKY KEQIIQGFY WAVKEGPLAE EPIYGVQYKL LSISVPSDVN IDVMKSQIIP LMKKACYVGL LTAIPILLEP IYEVDITVHA PLLPIVEELM KKRRGSRIY KTIKVAGTPL LEVRGQVPVI ESAGFETDLR LSTNGLGMCQ LYFWHKIWRK VPGDVLDKDA FIPKLKPAPI N SLSRDFVM KTRRRKGIST GGFMSNDGPT LEKYISAELY AQLRENGLVP

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Macromolecule #11: Spliceosomal protein DIB1

MacromoleculeName: Spliceosomal protein DIB1 / type: protein_or_peptide / ID: 11 / Details: Spliceosomal protein DIB1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 16.798387 KDa
SequenceString:
MASVLLPQLR TGWHVDQAIV TETKRLVVIR FGRKNDRQCM IMDELLSSIA ERVRNFAVIY LCDIDEVSDF DEMYELTDPM TVMFFYHNK HMMCDFGTGN NNKLNFIVDD KQEMIDILET IFRGARKNKG LVVSPYDYNH KRVS

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Macromolecule #12: 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component

MacromoleculeName: 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component
type: protein_or_peptide / ID: 12
Details: 66 kDa U4/U6.U5 small nuclear ribonucleoprotein component
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 66.544047 KDa
SequenceString: MNKTENLSIE ETNEIREKLG MKPIPVFQEK NTDHKESLSI EETNELRASL GLKLIPPQQN FNSSPPNVHN TSKIDELREK ITKFQKKAN APLRMAHLLE ETNVNDDSSW LENMDAIPSS HESKRSSTLP RKGATKEDEN IDLHNVQVSY NIEALSPKKD T ILTLKESS ...String:
MNKTENLSIE ETNEIREKLG MKPIPVFQEK NTDHKESLSI EETNELRASL GLKLIPPQQN FNSSPPNVHN TSKIDELREK ITKFQKKAN APLRMAHLLE ETNVNDDSSW LENMDAIPSS HESKRSSTLP RKGATKEDEN IDLHNVQVSY NIEALSPKKD T ILTLKESS IFDDTDSTEV LENVKAAEEN ADREKLRLRQ MNKDRRQKKK ILNVSSLDIE EEEEGEKHSI TTTHLIIGAE QG VMKAPNT ISAKPPTGKV KVNFDSANNM SDEDGGDFKP LKIKKRKIKD PRSTKARKSK ITDKMEIVKL VDEDESLSWM EEE QPVTII NPRTSSNNEL KGPEDLAREI EKARDEEKRR TESILKMREI SNSIVVDEKV TFLNTLDTSL SERSATENKV KVHG EGEKN IGDVTNGHTK EGSGNNTLTE AVNNEPNYEG DAENAPNFFS GLASTLGYLR KKSVFTTGDV DLKPGKDVNN SESLR RDVR NKEHTGTGTY TKDKLHGLEQ FTSSDSSNAN THSKRQDHYD PDIKLVYRDE KGNRLTTKEA YKKLSQKFHG TKSNKK KRA KMKSRIEARK NTPENGSLFE FDDN

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Macromolecule #13: Pre-mRNA-processing factor 31

MacromoleculeName: Pre-mRNA-processing factor 31 / type: protein_or_peptide / ID: 13 / Details: Pre-mRNA-processing factor 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 56.382516 KDa
SequenceString: MSSEEDYFDE LEYDLADEVN EEKEDIQTKK LTTVNCQTEK FNPFEILPES IELFRTLALI SPDRLSLSET AQILPKIVDL KRILQQQEI DFIKLLPFFN EIIPLIKSNI KLMHNFLISL YSRRFPELSS LIPSPLQYSK VISILENENY SKNESDELFF H LENKAKLT ...String:
MSSEEDYFDE LEYDLADEVN EEKEDIQTKK LTTVNCQTEK FNPFEILPES IELFRTLALI SPDRLSLSET AQILPKIVDL KRILQQQEI DFIKLLPFFN EIIPLIKSNI KLMHNFLISL YSRRFPELSS LIPSPLQYSK VISILENENY SKNESDELFF H LENKAKLT REQILVLTMS MKTSFKNKEP LDIKTRTQIL EANSILENLW KLQEDIGQYI ASKISIIAPN VCFLVGPEIA AQ LIAHAGG VLEFSRIPSC NIASIGKNKH LSHELHTLES GVRQEGYLFA SDMIQKFPVS VHKQMLRMLC AKVSLAARVD AGQ KNGDRN TVLAHKWKAE LSKKARKLSE APSISETKAL PIPEDQPKKK RAGRKFRKYK EKFRLSHVRQ LQNRMEFGKQ EQTV LDSYG EEVGLGMSNT SLQQAVGATS GSRRSAGNQA KLTKVMKHRI SEANQQADEF LISLGHNTEQ PNLSPEMVQM HKKQH TNPE EETNWFSGHG

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Macromolecule #14: U4/U6 small nuclear ribonucleoprotein PRP3

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein PRP3 / type: protein_or_peptide / ID: 14 / Details: U4/U6 small nuclear ribonucleoprotein PRP3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 55.97432 KDa
SequenceString: MPPRNTYEKG NPKRQNSPYY KPSFLRREET TNDEEKFQGH GLKTELHSAL KSSNLNLIRR TYQTGENPYL SDPHDRGSSS RFNRRYERG LKFYQKGEIS KRIAQERTLQ KQQEEEELKR KLKQEEDEKD KRKLIESGDL PNLELHEDKF LLDLSKFKIY Y DNNHGYEW ...String:
MPPRNTYEKG NPKRQNSPYY KPSFLRREET TNDEEKFQGH GLKTELHSAL KSSNLNLIRR TYQTGENPYL SDPHDRGSSS RFNRRYERG LKFYQKGEIS KRIAQERTLQ KQQEEEELKR KLKQEEDEKD KRKLIESGDL PNLELHEDKF LLDLSKFKIY Y DNNHGYEW WDTAYLDEKG ELMEKYDMNG TSPAEEKLAE DIDEVDDDDD DEHPSIRYVA HPLPEKINEA KVSIKAYLTQ HE RKRLRRN RRKMAREARE IKIKLGLLPK PEPKVKLSNM MSVFENDQNI TDPTAWEKVV KDQVDLRKRK HLEENERRHE DAI KRRKEA VNMNVEKPTV YHCKVFQFKN LQNPKIRFKL KMNSKELSLK GLCLRIRDDG PGIIIVVGNE KSCKFYENLV MKRI KWNED FELHTNTGDI KMDMHNNSIS KTWEGYLQDC KFKGWFMKVC NDQDSLLRTL GQFDSEHFYS PVQT

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Macromolecule #15: U4/U6 small nuclear ribonucleoprotein PRP4

MacromoleculeName: U4/U6 small nuclear ribonucleoprotein PRP4 / type: protein_or_peptide / ID: 15 / Details: U4/U6 small nuclear ribonucleoprotein PRP4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 52.506984 KDa
SequenceString: MSKYIALENL PVDLQHKGAT QNESTADILK QLPHERLQAV LEKIPEEDLE VRRLLSILKK PEVVENEDVQ QRRIRLAEIL MVDEIDLEN INNMENINGE EVDEEDDEDF FTPATSELIF ARRFLINYSL ERSRKRLQKE MERHQKFNTR QELLSRRTEL Q RMANLELA ...String:
MSKYIALENL PVDLQHKGAT QNESTADILK QLPHERLQAV LEKIPEEDLE VRRLLSILKK PEVVENEDVQ QRRIRLAEIL MVDEIDLEN INNMENINGE EVDEEDDEDF FTPATSELIF ARRFLINYSL ERSRKRLQKE MERHQKFNTR QELLSRRTEL Q RMANLELA GSQLVSTKPI SAVSLSTDDM VVATGSWAGD LQVLNSQTLQ PLTQKLDSHV GKIGAIDWHP DSNNQMISCA ED GLIKNFQ YSNEEGGLRL LGDLVGHERR ISDVKYHPSG KFIGSASHDM TWRLWDASTH QELLLQEGHD KGVFSLSFQC DGS LVCSGG MDSLSMLWDI RSGSKVMTLA GHSKPIYTVA WSPNGYQVAT GGGDGIINVW DIRKRDEGQL NQILAHRNIV TQVR FSKED GGKKLVSCGY DNLINVYSSD TWLKMGSLAG HTDKIISLDI SNNSHFLVSG GWDRSIKLWN

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Macromolecule #17: Pre-mRNA-splicing factor 6

MacromoleculeName: Pre-mRNA-splicing factor 6 / type: protein_or_peptide / ID: 17 / Details: Pre-mRNA-splicing factor 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 104.370133 KDa
SequenceString: MERPSFLDQE PPAGYVPGIG RGATGFSTKE KQVVSNDDKG RRIPKRYREN LNNHLQSQPK DDEDDEAANV FKTLELKLAQ KKKKRANEK DDDNSVDSSN VKRQFADLKE SLAAVTESEW MDIPDATDFT RRNKRNRIQE QLNRKTYAAP DSLIPGNVDL N KLTEEREK ...String:
MERPSFLDQE PPAGYVPGIG RGATGFSTKE KQVVSNDDKG RRIPKRYREN LNNHLQSQPK DDEDDEAANV FKTLELKLAQ KKKKRANEK DDDNSVDSSN VKRQFADLKE SLAAVTESEW MDIPDATDFT RRNKRNRIQE QLNRKTYAAP DSLIPGNVDL N KLTEEREK LLQSQIDENL AQLTKNASNP IQVNKPNAAT DALSYLKDLE NDRVNSLSDA TLEDLQKMRT ILKSYRKADP TN PQGWIAS ARLEEKARKF SVAKKIIENG CQECPRSSDI WLENIRLHES DVHYCKTLVA TAINFNPTSP LLWFKAIDLE STT VNKYRV VRKALQEIPR DEGLWKLAVS FEADKAQVIK MLEKATQFIP QSMDLLTAYT NLQSYHNAKM TLNSFRKILP QEPE IWIIS TLLEERNNPD IPVDKLVSLL KEGLLELSKN GYKATLSAWL KRAEALNDAP NSNLTCQAIV YAILEWLRES GEYES ELNN VDQILEKMPH SKVQIAVLKK LIQWDPCDTV LWSRLKMATE SYHKIEELLA FFQELLFQTK NSDDIRANMR EKSPGL LMM YVSEYWKAQK GDTRQTLVLI DQIIDFAPHN LDLRFFKIKL LGRSLQLDEL RDFFQQTFSS LEDFKISGTE RLYYKYV NF LRYQDLNEEA IKFLNERCLK SFPICHKFFL QLGQIYHSMG NIEMSRETYL SGTRLVPNCP LLWVSLSKID EIDLKNPV R ARSILDRGLL KNPDDVLFYI AKIQMEIRLG NLDQAELLVT QALQKFPSNA LLWVEQIKLF KHGNKSSLKK TIFQDALRR TQNDHRVLLE IGVSFYAEAQ YETSLKWLER ALKKCSRYGD TWVWLFRTYA RLGKDTVDLY NMFDQCEPTY GPEWIAASKN VKMQYCTPR EILLRLMNDK

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Macromolecule #18: 13 kDa ribonucleoprotein-associated protein

MacromoleculeName: 13 kDa ribonucleoprotein-associated protein / type: protein_or_peptide / ID: 18 / Details: 13 kDa ribonucleoprotein-associated protein / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 13.582855 KDa
SequenceString:
MSAPNPKAFP LADAALTQQI LDVVQQAANL RQLKKGANEA TKTLNRGISE FIIMAADCEP IEILLHLPLL CEDKNVPYVF VPSRVALGR ACGVSRPVIA ASITTNDASA IKTQIYAVKD KIETLLI

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Macromolecule #19: 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component

MacromoleculeName: 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component
type: protein_or_peptide / ID: 19
Details: 23 kDa U4/U6.U5 small nuclear ribonucleoprotein component
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 22.717799 KDa
SequenceString: MSNFGRRTWD REEYAEQARS GYDDRSLKAT LTPIELQALK SKYTNYDHLI KGSLKDLNKR KLTANTESLS SFKRGKKFGF YCDICNLTF KDTLQYIDHL NHKVHAIKFE NLFDEPLIID IRDNDDVPQE EFELCYHNLI KDFVEVRSME TQSKRKRLLD T DVEKAKKV ...String:
MSNFGRRTWD REEYAEQARS GYDDRSLKAT LTPIELQALK SKYTNYDHLI KGSLKDLNKR KLTANTESLS SFKRGKKFGF YCDICNLTF KDTLQYIDHL NHKVHAIKFE NLFDEPLIID IRDNDDVPQE EFELCYHNLI KDFVEVRSME TQSKRKRLLD T DVEKAKKV ATKPSIESES KVSQMMGFSN FATSKK

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Macromolecule #20: Pre-mRNA-splicing factor 38

MacromoleculeName: Pre-mRNA-splicing factor 38 / type: protein_or_peptide / ID: 20 / Details: Pre-mRNA-splicing factor 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 27.995619 KDa
SequenceString: MAVNEFQVES NISPKQLNNQ SVSLVIPRLT RDKIHNSMYY KVNLSNESLR GNTMVELLKV MIGAFGTIKG QNGHLHMMVL GGIEFKCIL MKLIEIRPNF QQLNFLLNVK NENGFDSKYI IALLLVYARL QYYYLNGNNK NDDDENDLIK LFKVQLYKYS Q HYFKLKSF ...String:
MAVNEFQVES NISPKQLNNQ SVSLVIPRLT RDKIHNSMYY KVNLSNESLR GNTMVELLKV MIGAFGTIKG QNGHLHMMVL GGIEFKCIL MKLIEIRPNF QQLNFLLNVK NENGFDSKYI IALLLVYARL QYYYLNGNNK NDDDENDLIK LFKVQLYKYS Q HYFKLKSF PLQVDCFAHS YNEELCIIHI DELVDWLATQ DHIWGIPLGK CQWNKIYNSD EESSSSESES NGDSEDDNDT SS ES

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Macromolecule #21: Pre-mRNA-splicing factor SPP381

MacromoleculeName: Pre-mRNA-splicing factor SPP381 / type: protein_or_peptide / ID: 21 / Details: Pre-mRNA-splicing factor SPP381 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 33.830926 KDa
SequenceString: MSFRHFKRRL DTSSADESSS ADEEHPDQNV SLTEKSASLS HSDLGGEILN GTGKNRTPND GQESNESDGS PESDESPESE ESSDNSDSS DSDDMRPLPR PLFMKKKANN LQKATKIDQP WNAQDDARVL QTKKENMIKN IDKANQVAKN YETMKLRLNT N YSTNEELI ...String:
MSFRHFKRRL DTSSADESSS ADEEHPDQNV SLTEKSASLS HSDLGGEILN GTGKNRTPND GQESNESDGS PESDESPESE ESSDNSDSS DSDDMRPLPR PLFMKKKANN LQKATKIDQP WNAQDDARVL QTKKENMIKN IDKANQVAKN YETMKLRLNT N YSTNEELI KQCLLLDDND EVDSEKERQK WFERQNERKQ KHRRIQLAKQ RESEEYEAKR FEAMQKGKDG NTKYDVILDK EK EKLDHKK QRSAEKVEKS HNNNRYKITR TKNVEFGDLG KNSRDYEETE YSVI

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Macromolecule #22: U2 snRNP component HSH155

MacromoleculeName: U2 snRNP component HSH155 / type: protein_or_peptide / ID: 22 / Details: U2 snRNP component HSH155 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 110.166672 KDa
SequenceString: MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR EDSYHKRRFD MKFEPDSDTQ TVTSSENTQ DAVVPRKRKS RWDVKGYEPP DESSTAVKEN SDSALVNVEG IHDLMFFKPS DHKYFADVIS KKPIDELNKD E KKERTLSM ...String:
MSHPIQFVNA NNSDKSHQLG GQYSIPQDLR ENLQKEAARI GENEKDVLQE KMETRTVQNR EDSYHKRRFD MKFEPDSDTQ TVTSSENTQ DAVVPRKRKS RWDVKGYEPP DESSTAVKEN SDSALVNVEG IHDLMFFKPS DHKYFADVIS KKPIDELNKD E KKERTLSM LLLKIKNGNT ASRRTSMRIL TDKAVTFGPE MIFNRLLPIL LDRSLEDQER HLMIKTIDRV LYQLGDLTKP YV HKILVVA APLLIDEDPM VRSTGQEIIT NLSTVAGLKT ILTVMRPDIE NEDEYVRNVT SRAAAVVAKA LGVNQLLPFI NAA CHSRKS WKARHTGIKI VQQIGILLGI GVLNHLTGLM SCIKDCLMDD HVPVRIVTAH TLSTLAENSY PYGIEVFNVV LEPL WKGIR SHRGKVLSSF LKAVGSMIPL MDPEYAGYYT TEAMRIIRRE FDSPDDEMKK TILLVLQKCS AVESITPKFL REEIA PEFF QKFWVRRVAL DRPLNKVVTY TTVTLAKKLG CSYTIDKLLT PLRDEAEPFR TMAVHAVTRT VNLLGTADLD ERLETR LID ALLIAFQEQT NSDSIIFKGF GAVTVSLDIR MKPFLAPIVS TILNHLKHKT PLVRQHAADL CAILIPVIKN CHEFEML NK LNIILYESLG EVYPEVLGSI INAMYCITSV MDLDKLQPPI NQILPTLTPI LRNKHRKVEV NTIKFVGLIG KLAPTYAP P KEWMRICFEL LELLKSTNKE IRRSANATFG FIAEAIGPHD VLVALLNNLK VQERQLRVCT AVAIGIVAKV CGPYNVLPV IMNEYTTPET NVQNGVLKAM SFMFEYIGNM SKDYIYFITP LLEDALTDRD LVHRQTASNV ITHLALNCSG TGHEDAFIHL MNLLIPNIF ETSPHAIMRI LEGLEALSQA LGPGLFMNYI WAGLFHPAKN VRKAFWRVYN NMYVMYQDAM VPFYPVTPDN N EEYIEELD LVL

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Macromolecule #23: Pre-mRNA-splicing factor RSE1

MacromoleculeName: Pre-mRNA-splicing factor RSE1 / type: protein_or_peptide / ID: 23 / Details: Pre-mRNA-splicing factor RSE1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 153.956781 KDa
SequenceString: MWGGGKMAVV SLSPHTAKMR KLFGQASTTM AYDGLKREAE RRTRSDHNIT MVAKDDELYL YHLTLKKQTN FVHSCIGHFV DLEAGSKRE QSQLCVATET HLELYDTADG ELKLIAKFQN LFATITSMKS LDLPHSGSRA KASNWPTFLA LTSDSGNLSI V QIIMHAGA ...String:
MWGGGKMAVV SLSPHTAKMR KLFGQASTTM AYDGLKREAE RRTRSDHNIT MVAKDDELYL YHLTLKKQTN FVHSCIGHFV DLEAGSKRE QSQLCVATET HLELYDTADG ELKLIAKFQN LFATITSMKS LDLPHSGSRA KASNWPTFLA LTSDSGNLSI V QIIMHAGA LRLKTLVNQP LTRTTLRRVS PISYMEIDPN GRCIILSSVE QNKLCFLVDY AQKLRISSPL EIIRPHMVTL DM AVVDVNF NNPCFVTLEI DNAATQLSVH LIFYVLELGL NHIVKKADYL VNPSANFVLS LPDLSRYNIT TSLSDNNYDA DYD TLFNPF VVIGFENHIL VKDMNGFFSL KVEIPKRSIT NSRHKNVTII SGIVQKLKND FFVLLQSNHG DLFKLTVSPD TNDR NRPLV QLSYFDTIQN SHQLHIFKNG YLFALSEMNN NFLFQFEKLG VEKNDFSNVL TSKDPNKSLV FEPSIKLQNL SILSQ QLNL NPSIKSQIVS DSPLSIATKH FTNNKIITLT NAVNYSNLIS TSLPPNATKL WLIPDPATTG DNNTLLFITF PKKTMI LQI DNESMEELTP DEATRSAFKL SQDTTIHTCL MGSHSIIQVC TAELRHIVPT GKSRYSNKLT WVPPAGIRIV CATSSKT QL IISLSNYELV YFKIDVSSDS LIELTTHPEL DTMPSKVAIV QDTQHADLLA IADNEGMIKI MSLKDQKEDF LTVISLQL V SEKISDMIMV RDSSIGQLNL HVGLENGVYM KFHIGDVDGS FTDIKRRFLG LKPVSLSYLR EISVSLNNEE EEEEEEDDD DEKEEEEINS SGAKWMSCVV CHSSSTWVSY TWKNVWTIRQ LKDQNMLSCS KFVNADVAIN GVCSISSSGR LNIGRVSNFP TLDNWFHVH ESSVNKQENG GGDESNEEEE DEMEEEMEML QISTFRPRTI LSFPNNPKSI LFIDNHSGKK QCRISLQIDG E CLKFGSSD HLYKILDDID CVSAAIIDFT RQADHLIICA GDKRLLTYKI LVNKDKLSFD IELLHQTEII SPIHAMLKFK NF LLTAMGS TIVLYGLGKK QLLRRSVTQT PVSITKIVSM HQWNYERLAV GDIHESVTLF IWDPAGNVFI PYVDDSVKRH VTV LKFLDE ATVIGADRYG NAWTLRSPPE CEKIMSNHDP SELSNGAIKY PLDVITLQQK LPNTYDCKFK FQLLNHFFVN DIIT DFHIL DSLSNSDRPG CIYMGLQGTV GCFIPLLSKG NVFMMGNIEN IMAEADDTFY LDYESRKKNN NMRKEDDEEE SGSVV LQGR HGIEDEIICE GSCSILGRDH QEYRSYYAPV RKVIDGDLCE NFLRLSLNEQ EFLAKNLKSV QVEDIIQTIN EVRTNY M

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Macromolecule #24: Cold sensitive U2 snRNA suppressor 1

MacromoleculeName: Cold sensitive U2 snRNA suppressor 1 / type: protein_or_peptide / ID: 24 / Details: Cold sensitive U2 snRNA suppressor 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 50.210699 KDa
SequenceString: MARTKSRKRS GNNQNKNASV VNNKAEIAAM IDARRLEQKK KGGVTNSKGK TNKVVDAKLE KEFKDVLQRF QVQENDTPKE ITKDEKNNH VVIVEKNPVM NRKHTAEDEL EDTPSDGIEE HLSARKRRKT EKPSLSQLKS QVPYPQIIEW YDCDARYPGL L ASIKCTKN ...String:
MARTKSRKRS GNNQNKNASV VNNKAEIAAM IDARRLEQKK KGGVTNSKGK TNKVVDAKLE KEFKDVLQRF QVQENDTPKE ITKDEKNNH VVIVEKNPVM NRKHTAEDEL EDTPSDGIEE HLSARKRRKT EKPSLSQLKS QVPYPQIIEW YDCDARYPGL L ASIKCTKN VIPVPSHWQS KKEYLSGRSL LGKRPFELPD IIKKTNIEQM RSTLPQSGLD GQDEKSLKEA SRARVQPKMG AL DLDYKKL HDVFFKIGAN WKPDHLLCFG DVYYENRNLF EETNWKRMVD HKRPGRISQE LRAIMNLPEG QLPPWCMKMK DIG LPTGYP DLKIAGLNWD ITNLKGDVYG KIIPNHHSRS KKQGRNYFGA LISFETPEFE NSKEDTQANA ENGRQDDKID DEVE HKLDH FQEDISEVTS AEEKLERNEE ESEKQLYTVL

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Macromolecule #25: Protein HSH49

MacromoleculeName: Protein HSH49 / type: protein_or_peptide / ID: 25 / Details: Protein HSH49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 24.534152 KDa
SequenceString: MNYSADSGNT VYVGNIDPRI TKEQLYELFI QINPVLRIKY PKDKVLQAYQ GYAFIEFYNQ GDAQYAIKIM NNTVRLYDRL IKVRQVTNS TGTTNLPSNI SKDMILPIAK LFIKNLADSI DSDQLVKIFN KFGKLIREPE IFYLSNGKLK CAYVYFEDFE K ADLAIKSL ...String:
MNYSADSGNT VYVGNIDPRI TKEQLYELFI QINPVLRIKY PKDKVLQAYQ GYAFIEFYNQ GDAQYAIKIM NNTVRLYDRL IKVRQVTNS TGTTNLPSNI SKDMILPIAK LFIKNLADSI DSDQLVKIFN KFGKLIREPE IFYLSNGKLK CAYVYFEDFE K ADLAIKSL NNQLVANNRI TVDYAFKENG KGNAKYGDDV DRLLNKEALK HNMLK

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Macromolecule #26: Pre-mRNA-splicing factor RDS3

MacromoleculeName: Pre-mRNA-splicing factor RDS3 / type: protein_or_peptide / ID: 26 / Details: Pre-mRNA-splicing factor RDS3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 12.283573 KDa
SequenceString:
MSRHQFDLIM CLKQPGVQTG LLCEKCDGKC PICDSYVRPK RKVRVCENCS FGKQAKNCII CNLNVGVNDA FYCWECCRLG KDKDGCPRI LNLGSNRLDR HFEKKKKV

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Macromolecule #27: Pre-mRNA-splicing factor PRP9

MacromoleculeName: Pre-mRNA-splicing factor PRP9 / type: protein_or_peptide / ID: 27 / Details: Pre-mRNA-splicing factor PRP9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 63.126445 KDa
SequenceString: MNLLETRRSL LEEMEIIENA IAERIQRNPE LYYHYIQESS KVFPDTKLPR SSLIAENKIY KFKKVKRKRK QIILQQHEIN IFLRDYQEK QQTFNKINRP EETQEDDKDL PNFERKLQQL EKELKNEDEN FELDINSKKD KYALFSSSSD PSRRTNILSD R ARDLDLNE ...String:
MNLLETRRSL LEEMEIIENA IAERIQRNPE LYYHYIQESS KVFPDTKLPR SSLIAENKIY KFKKVKRKRK QIILQQHEIN IFLRDYQEK QQTFNKINRP EETQEDDKDL PNFERKLQQL EKELKNEDEN FELDINSKKD KYALFSSSSD PSRRTNILSD R ARDLDLNE IFTRDEQYGE YMELEQFHSL WLNVIKRGDC SLLQFLDILE LFLDDEKYLL TPPMDRKNDR YMAFLLKLSK YV ETFFFKS YALLDAAAVE NLIKSDFEHS YCRGSLRSEA KGIYCPFCSR WFKTSSVFES HLVGKIHKKN ESKRRNFVYS EYK LHRYLK YLNDEFSRTR SFVERKLAFT ANERMAEMDI LTQKYEAPAY DSTEKEGAEQ VDGEQRDGQL QEEHLSGKSF DMPL GPDGL PMPYWLYKLH GLDREYRCEI CSNKVYNGRR TFERHFNEER HIYHLRCLGI EPSSVFKGIT KIKEAQELWK NMQGQ SQLT SIAAVPPKPN PSQLKVPTEL ELEEEDEEGN VMSKKVYDEL KKQGLV

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Macromolecule #28: Pre-mRNA-splicing factor PRP11

MacromoleculeName: Pre-mRNA-splicing factor PRP11 / type: protein_or_peptide / ID: 28 / Details: Pre-mRNA-splicing factor PRP11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 29.962809 KDa
SequenceString: MNYLEGVGSK KGGGGIASES QFNLQRRKEV ESLLSKGENV PYTFQDEKDD QVRSNPYIYK NHSGKLVCKL CNTMHMSWSS VERHLGGKK HGLNVLRRGI SIEKSSLGRE GQTTHDFRQQ QKIIEAKQSL KNNGTIPVCK IATVKNPKNG SVGLAIQVNY S SEVKENSV ...String:
MNYLEGVGSK KGGGGIASES QFNLQRRKEV ESLLSKGENV PYTFQDEKDD QVRSNPYIYK NHSGKLVCKL CNTMHMSWSS VERHLGGKK HGLNVLRRGI SIEKSSLGRE GQTTHDFRQQ QKIIEAKQSL KNNGTIPVCK IATVKNPKNG SVGLAIQVNY S SEVKENSV DSDDKAKVPP LIRIVSGLEL SDTKQKGKKF LVIAYEPFEN IAIELPPNEI LFSENNDMDN NNDGVDELNK KC TFWDAIS KLYYVQFFFK QAEQEQADV

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Macromolecule #29: Pre-mRNA-splicing factor PRP21

MacromoleculeName: Pre-mRNA-splicing factor PRP21 / type: protein_or_peptide / ID: 29 / Details: Pre-mRNA-splicing factor PRP21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 33.111512 KDa
SequenceString: MEPEDTQLKE DIKTTVNYIK QHGVEFENKL LEDERFSFIK KDDPLHEYYT KLMNEPTDTV SGEDNDRKSE REIARPPDFL FSQYDTGIS RRDMEVIKLT ARYYAKDKSI VEQMISKDGE ARLNFMNSSH PLHKTFTDFV AQYKRVYSFT GQEIKKSKRT I LDNCFERT ...String:
MEPEDTQLKE DIKTTVNYIK QHGVEFENKL LEDERFSFIK KDDPLHEYYT KLMNEPTDTV SGEDNDRKSE REIARPPDFL FSQYDTGIS RRDMEVIKLT ARYYAKDKSI VEQMISKDGE ARLNFMNSSH PLHKTFTDFV AQYKRVYSFT GQEIKKSKRT I LDNCFERT QYWEFEKDKD REHDKLVELC KIQFAAIPWD KFTQVAKFSI PEDTEIFEGS LDLEQMRLRR VQTGIKLFDS IK PTNEEEK IVSDQGKQKG GDSKGKKRKI RAVGETRLKK SKK

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Macromolecule #30: U2 small nuclear ribonucleoprotein A'

MacromoleculeName: U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 30 / Details: U2 small nuclear ribonucleoprotein A' / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 27.232252 KDa
SequenceString: MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL TKPTHILDLT NNDLIMIPDL SRRDDIHTLL LGRNNIVEV DGRLLPMNVQ NLTLSNNSIR RFEDLQRLRR APRTLKNLTL IGNQVCHLAN YREHVLRLVP HLETLDFQNV T AEERKSAM ...String:
MKFTPSIVID APQYYVDHFN GKYNVDKCVI LRDLQLETDS ESMPSSLKHL TKPTHILDLT NNDLIMIPDL SRRDDIHTLL LGRNNIVEV DGRLLPMNVQ NLTLSNNSIR RFEDLQRLRR APRTLKNLTL IGNQVCHLAN YREHVLRLVP HLETLDFQNV T AEERKSAM SFPRQADGDT LGPVNTAIRD NGSRDKTMEI MNLVVSKMTV ERRNELKKQL AEATSLEEIA RLEKLLSGGV

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Macromolecule #31: Unknown

MacromoleculeName: Unknown / type: protein_or_peptide / ID: 31 / Details: Unknown protein helices within B complex / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 7.907637 KDa
SequenceString:
AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA

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Macromolecule #32: U2 small nuclear ribonucleoprotein B''

MacromoleculeName: U2 small nuclear ribonucleoprotein B'' / type: protein_or_peptide / ID: 32 / Details: U2 small nuclear ribonucleoprotein B'' / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 12.850944 KDa
SequenceString:
MVEPARKKQR IDRDTHHTVA EPVTEAKNTL YVSQLNEKIN MQRLRVNLFL LFATFGEVLK VSMNFKKQRG QAFITMRTID QASLAQISL NGERFFGKPL KVEFSKSETK TL

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Macromolecule #33: RDS3 complex subunit 10

MacromoleculeName: RDS3 complex subunit 10 / type: protein_or_peptide / ID: 33 / Details: RDS3 complex subunit 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 10.045401 KDa
SequenceString:
MAEKQRQLKL QKIYKQKYIG LGDESTTREQ WQRNVRNDTL NTLQGHSASL EYVSLSRGDL SIRDTRIHLL KSMSPGYKAY LREER

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Macromolecule #34: U6 snRNA-associated Sm-like protein LSm2

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm2 / type: protein_or_peptide / ID: 34 / Details: U6 snRNA-associated Sm-like protein LSm2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 11.177888 KDa
SequenceString:
MLFFSFFKTL VDQEVVVELK NDIEIKGTLQ SVDQFLNLKL DNISCTDEKK YPHLGSVRNI FIRGSTVRYV YLNKNMVDTN LLQDATRRE VMTERK

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Macromolecule #35: Small nuclear ribonucleoprotein-associated protein B

MacromoleculeName: Small nuclear ribonucleoprotein-associated protein B / type: protein_or_peptide / ID: 35
Details: Small nuclear ribonucleoprotein-associated protein B
Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 22.42699 KDa
SequenceString: MSKIQVAHSS RLANLIDYKL RVLTQDGRVY IGQLMAFDKH MNLVLNECIE ERVPKTQLDK LRPRKDSKDG TTLNIKVEKR VLGLTILRG EQILSTVVED KPLLSKKERL VRDKKEKKQA QKQTKLRKEK EKKPGKIAKP NTANAKHTSS NSREIAQPSS S RYNGGNDN ...String:
MSKIQVAHSS RLANLIDYKL RVLTQDGRVY IGQLMAFDKH MNLVLNECIE ERVPKTQLDK LRPRKDSKDG TTLNIKVEKR VLGLTILRG EQILSTVVED KPLLSKKERL VRDKKEKKQA QKQTKLRKEK EKKPGKIAKP NTANAKHTSS NSREIAQPSS S RYNGGNDN IGANRSRFNN EAPPQTRKFQ PPPGFKRK

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Macromolecule #36: Small nuclear ribonucleoprotein Sm D3

MacromoleculeName: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 36 / Details: Small nuclear ribonucleoprotein Sm D3 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 11.240139 KDa
SequenceString:
MTMNGIPVKL LNEAQGHIVS LELTTGATYR GKLVESEDSM NVQLRDVIAT EPQGAVTHMD QIFVRGSQIK FIVVPDLLKN APLFKKNSS RPMPPIRGPK RR

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Macromolecule #37: Small nuclear ribonucleoprotein E

MacromoleculeName: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 37 / Details: Small nuclear ribonucleoprotein E / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 10.385098 KDa
SequenceString:
MSNKVKTKAM VPPINCIFNF LQQQTPVTIW LFEQIGIRIK GKIVGFDEFM NVVIDEAVEI PVNSADGKED VEKGTPLGKI LLKGDNITL ITSAD

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Macromolecule #38: Small nuclear ribonucleoprotein F

MacromoleculeName: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 38 / Details: Small nuclear ribonucleoprotein F / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 9.669945 KDa
SequenceString:
MSESSDISAM QPVNPKPFLK GLVNHRVGVK LKFNSTEYRG TLVSTDNYFN LQLNEAEEFV AGVSHGTLGE IFIRCNNVLY IRELPN

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Macromolecule #39: Small nuclear ribonucleoprotein G

MacromoleculeName: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 39 / Details: Small nuclear ribonucleoprotein G / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 8.490809 KDa
SequenceString:
MVSTPELKKY MDKKILLNIN GSRKVAGILR GYDIFLNVVL DDAMEINGED PANNHQLGLQ TVIRGNSIIS LEALDAI

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Macromolecule #40: Small nuclear ribonucleoprotein Sm D1

MacromoleculeName: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 40 / Details: Small nuclear ribonucleoprotein Sm D1 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 16.296798 KDa
SequenceString:
MKLVNFLKKL RNEQVTIELK NGTTVWGTLQ SVSPQMNAIL TDVKLTLPQP RLNKLNSNGI AMASLYLTGG QQPTASDNIA SLQYINIRG NTIRQIILPD SLNLDSLLVD QKQLNSLRRS GQIANDPSKK RRRDFGAPAN KRPRRGL

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Macromolecule #41: Small nuclear ribonucleoprotein Sm D2

MacromoleculeName: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 41 / Details: Small nuclear ribonucleoprotein Sm D2 / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 12.876066 KDa
SequenceString:
MSSQIIDRPK HELSRAELEE LEEFEFKHGP MSLINDAMVT RTPVIISLRN NHKIIARVKA FDRHCNMVLE NVKELWTEKK GKNVINRER FISKLFLRGD SVIVVLKTPV E

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Macromolecule #42: U6 snRNA-associated Sm-like protein LSm4

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm4 / type: protein_or_peptide / ID: 42 / Details: U6 snRNA-associated Sm-like protein LSm4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 21.29807 KDa
SequenceString:
MLPLYLLTNA KGQQMQIELK NGEIIQGILT NVDNWMNLTL SNVTEYSEES AINSEDNAES SKAVKLNEIY IRGTFIKFIK LQDNIIDKV KQQINSNNNS NSNGPGHKRY YNNRDSNNNR GNYNRRNNNN GNSNRRPYSQ NRQYNNSNSS NINNSINSIN S NNQNMNNG LGGSVQHHFN SSSPQKVEF

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Macromolecule #43: U6 snRNA-associated Sm-like protein LSm5

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm5 / type: protein_or_peptide / ID: 43 / Details: U6 snRNA-associated Sm-like protein LSm5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 10.432954 KDa
SequenceString:
MSLPEILPLE VIDKTINQKV LIVLQSNREF EGTLVGFDDF VNVILEDAVE WLIDPEDESR NEKVMQHHGR MLLSGNNIAI LVPGGKKTP TEAL

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Macromolecule #44: U6 snRNA-associated Sm-like protein LSm6

MacromoleculeName: U6 snRNA-associated Sm-like protein LSm6 / type: protein_or_peptide / ID: 44 / Details: U6 snRNA-associated Sm-like protein LSm6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Baker's yeast (brewer's yeast)
Molecular weightTheoretical: 9.406579 KDa
SequenceString:
MSGKASTEGS VTTEFLSDII GKTVNVKLAS GLLYSGRLES IDGFMNVALS SATEHYESNN NKLLNKFNSD VFLRGTQVMY ISEQKI

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Macromolecule #45: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 45 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

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Macromolecule #46: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 46 / Number of copies: 7 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.5 mg/mL
BufferpH: 7.9
Component:
ConcentrationFormula
20.0 mMHEPES
50.0 mMKCl
0.2 mMEDTAEthylenediaminetetraacetic acid
1.0 mMDTT
2.5 mMDesthiobiotin

Details: Buffer pH: HEPES, 7.9; EDTA, 8.0
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 15 sec. / Pretreatment - Atmosphere: AIR
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK III
Details: Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ...Details: Grids were glow-discharged for 15 s before deposition of 3 microliter sample (~1.5 mg mL-1), and subsequently incubated for 2-3.5 s before blotting and vitrification by plunging into liquid ethane with a Vitrobot Mark III (FEI) operated at 4 degrees Celsius and 100% humidity..

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated defocus max: 5.3 µm / Calibrated defocus min: 0.35000000000000003 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal magnification: 81000
Specialist opticsEnergy filter - Name: GIF Quantum
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Dimensions - Width: 3710 pixel / Digitization - Dimensions - Height: 3838 pixel / Digitization - Frames/image: 1-20 / Number real images: 5115 / Average exposure time: 16.0 sec. / Average electron dose: 56.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 496581
Startup modelType of model: OTHER
Details: The negative stain map of human B-delta-U1 (EMD-1066) was used as an initial reference for three-dimensional refinement of a 12229 particle negative stain data set of the yeast B complex. A ...Details: The negative stain map of human B-delta-U1 (EMD-1066) was used as an initial reference for three-dimensional refinement of a 12229 particle negative stain data set of the yeast B complex. A single round of 3D classification revealed a B complex density from 3707 particles that was refined to an estimated resolution of 50 A. This density was low-pass filtered to 60 A and used for processing of cryo-EM data.
Initial angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 2.0)
Final 3D classificationSoftware - Name: RELION (ver. 2.0)
Final angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 2.0)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 7.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.0)
Details: A temperature factor of -170 A2 was applied (sharpened B2 map).
Number images used: 9559
DetailsMovies were binned once and aligned using MOTIONCORR.

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Atomic model buiding 1

RefinementSpace: REAL / Overall B value: 170
Output model

PDB-5nrl:
Structure of a pre-catalytic spliceosome.

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