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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-30843 | |||||||||
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Title | Complex of FMDV and M8 Nab | |||||||||
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Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.9 Å | |||||||||
![]() | Dong H / Liu P | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and molecular basis for foot-and-mouth disease virus neutralization by two potent protective antibodies. Authors: Hu Dong / Pan Liu / Manyuan Bai / Kang Wang / Rui Feng / Dandan Zhu / Yao Sun / Suyu Mu / Haozhou Li / Michiel Harmsen / Shiqi Sun / Xiangxi Wang / Huichen Guo / ![]() ![]() | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 226.2 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.1 KB 14.1 KB | Display Display | ![]() |
Images | ![]() | 71.7 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7dssMC ![]() 7dstC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 1.35 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : FMDV in complex with M8 Nab
Entire | Name: FMDV in complex with M8 Nab |
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Components |
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-Supramolecule #1: FMDV in complex with M8 Nab
Supramolecule | Name: FMDV in complex with M8 Nab / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
-Supramolecule #2: M8 Nab
Supramolecule | Name: M8 Nab / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
-Supramolecule #3: FMDV
Supramolecule | Name: FMDV / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2-#5 |
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-Macromolecule #1: M8 Nab
Macromolecule | Name: M8 Nab / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 13.932477 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: GGSQVQLQES GGGLVQPGGS LRLSCVASGT VFSINDISIN HLGWYRQAPG KERELVAAIT ADGTSAYEDS VKGRFIISRD DAKKMVYLQ MNSLKPEDTA VYYCNGLRAS NAGWEPRFGT WGQGTQVTVS S |
-Macromolecule #2: VP1 of O type FMDV capsid
Macromolecule | Name: VP1 of O type FMDV capsid / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 22.849139 KDa |
Sequence | String: TTSTGESADP VTATVENYGG ETQVQRRHHT DVSFILDRFV KVTPKDSINV LDLMQTPSHT LVGALLRTAT YYFADLEVAV KHKGDLTWV PNGAPVAALD NTTNPTAYHK APLTRLALPY TAPHRVLATV YNGKCKYAEG SLPNVRGDLQ VLAQKAARPL P TSFNYGAI ...String: TTSTGESADP VTATVENYGG ETQVQRRHHT DVSFILDRFV KVTPKDSINV LDLMQTPSHT LVGALLRTAT YYFADLEVAV KHKGDLTWV PNGAPVAALD NTTNPTAYHK APLTRLALPY TAPHRVLATV YNGKCKYAEG SLPNVRGDLQ VLAQKAARPL P TSFNYGAI KATRVTELLY RMKRAETYCP RPLLAVHPSA ARHKQKIVAP |
-Macromolecule #3: VP2 of O-type FMDV capsid
Macromolecule | Name: VP2 of O-type FMDV capsid / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 23.091078 KDa |
Sequence | String: RILTTRNGHT TSTTQSSVGI THGYATAEDF VNGPNTSGLE TRVVQAERFF KTHLFDWVTS DPFGRYYLLE LPTDHKGVYG SLTDSYAYM RNGWDVEVTA VGNQFNGGCL LVAMVPELCS IEQRELFQLT LFPHQFINPR TNMTAHIKVP FVGVNRYDQY K VHKPWTLV ...String: RILTTRNGHT TSTTQSSVGI THGYATAEDF VNGPNTSGLE TRVVQAERFF KTHLFDWVTS DPFGRYYLLE LPTDHKGVYG SLTDSYAYM RNGWDVEVTA VGNQFNGGCL LVAMVPELCS IEQRELFQLT LFPHQFINPR TNMTAHIKVP FVGVNRYDQY K VHKPWTLV VMVVAPLTVN TEGAPQIKVY ANIAPTNVHV AGEFPSKE |
-Macromolecule #4: VP3 of O-type FMDV capsid
Macromolecule | Name: VP3 of O-type FMDV capsid / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 23.704688 KDa |
Sequence | String: GIFPVACSDG YGGLVTTDPK TADPVYGKVF NPPRNMLPGR FTNLLDVAEA CPTFLHFDGD VPYVTTKTDS DRVLAQFDLS LAAKHMSNT FLAGLAQYYT QYSGTVNLHF MFTGPTDAKA RYMIAIAPPG MEPPKTPEAA AHCIHAEWDT GLNSKFTFSI P YLSAADYA ...String: GIFPVACSDG YGGLVTTDPK TADPVYGKVF NPPRNMLPGR FTNLLDVAEA CPTFLHFDGD VPYVTTKTDS DRVLAQFDLS LAAKHMSNT FLAGLAQYYT QYSGTVNLHF MFTGPTDAKA RYMIAIAPPG MEPPKTPEAA AHCIHAEWDT GLNSKFTFSI P YLSAADYA YTASDAAETT NVQGWVCLFQ ITHGKAEGDA LVVLASAGKD FELRLPVDAR Q |
-Macromolecule #5: VP4 of O-type FMDV capsid
Macromolecule | Name: VP4 of O-type FMDV capsid / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 7.963328 KDa |
Sequence | String: SQNQSGNTGS IINNYYMQQY QNSMDTQLGN NAISGGSNEG STDTTSTHTT NTQNNDWFSK LASSAFSGLF GALLA |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TALOS ARCTICA |
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Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 30.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: DARK FIELD |
Experimental equipment | ![]() Model: Talos Arctica / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 3000 |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |