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- EMDB-2917: EM structure of ribosome-SRP-FtsY complex in "closed" state -

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Basic information

Entry
Database: EMDB / ID: EMD-2917
TitleEM structure of ribosome-SRP-FtsY complex in "closed" state
Map dataReconstruction of ribosome-SRP-FtsY complex in the "closed" state. The map has been b-factor sharpened. The authors report resolution of 5.7 angstrom according to FSC=0.143, which was obtained from RELION post-process (for b-factor sharpening). Since the RELION post-process does not generate new half maps, the maps uploaded for FSC validation were the half maps before b-factor sharpening.
Sample
  • Sample: E. coli SRP-FtsY binds to E. coli ribosome with Lep50 nascent chain
  • Complex: E. coli 70S ribosome displaying Lep50 nascent chain
  • Protein or peptide: Signal recongnition particle and SRP receptor
Keywordsprotein targeting / signal recognition particle / signal sequence / ribosome
Function / homology
Function and homology information


signal recognition particle / signal-recognition-particle GTPase / 7S RNA binding / SRP-dependent cotranslational protein targeting to membrane / protein targeting to membrane / negative regulation of cytoplasmic translational initiation / stringent response / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity ...signal recognition particle / signal-recognition-particle GTPase / 7S RNA binding / SRP-dependent cotranslational protein targeting to membrane / protein targeting to membrane / negative regulation of cytoplasmic translational initiation / stringent response / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis / translational termination / negative regulation of cytoplasmic translation / DnaA-L2 complex / translation repressor activity / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / assembly of large subunit precursor of preribosome / ribosome assembly / cytosolic ribosome assembly / response to reactive oxygen species / translational initiation / regulation of cell growth / DNA-templated transcription termination / response to radiation / mRNA 5'-UTR binding / large ribosomal subunit / ribosome binding / transferase activity / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / response to antibiotic / negative regulation of DNA-templated transcription / mRNA binding / GTPase activity / GTP binding / ATP hydrolysis activity / DNA binding / RNA binding / zinc ion binding / cytosol / cytoplasm
Similarity search - Function
Signal recognition particle protein / Signal recognition particle, SRP54 subunit / Signal recognition particle, SRP54 subunit, M-domain / Signal recognition particle, SRP54 subunit, M-domain superfamily / Signal peptide binding domain / SRP54-type proteins GTP-binding domain signature. / Signal recognition particle SRP54, helical bundle / Signal recognition particle SRP54, N-terminal domain superfamily / SRP54-type protein, helical bundle domain / SRP54-type protein, helical bundle domain ...Signal recognition particle protein / Signal recognition particle, SRP54 subunit / Signal recognition particle, SRP54 subunit, M-domain / Signal recognition particle, SRP54 subunit, M-domain superfamily / Signal peptide binding domain / SRP54-type proteins GTP-binding domain signature. / Signal recognition particle SRP54, helical bundle / Signal recognition particle SRP54, N-terminal domain superfamily / SRP54-type protein, helical bundle domain / SRP54-type protein, helical bundle domain / Signal recognition particle, SRP54 subunit, GTPase domain / SRP54-type protein, GTPase domain / SRP54-type protein, GTPase domain / Ribosomal protein L11, bacterial-type / Ribosomal protein L25, short-form / Ribosomal protein L31 type A / Ribosomal protein L31 signature. / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / : / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L5, bacterial-type / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal protein L33 superfamily / Ribosomal protein L16 / Ribosomal protein L30, bacterial-type / L28p-like / : / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L20 / Ribosomal L32p protein family / Ribosomal protein L19 / Ribosomal protein L19 / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L19 superfamily / Ribosomal protein L21
Similarity search - Domain/homology
Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL34 ...Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL36A / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL6 / Signal recognition particle protein / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein bL25
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.7 Å
Authorsvon Loeffelholz O / Jiang Q / Ariosa A / Karuppasamy M / Huard K / Berger I / Shan S / Schaffitzel C
CitationJournal: PROC.NAT.ACAD.SCI.USA / Year: 2015
Title: Ribosome-SRP-FtsY cotranslational targeting complex in the closed state.
Authors: von Loeffelholz O / Jiang Q / Ariosa A / Karuppasamy M / Huard K / Berger I / Shan SO / Schaffitzel C
History
DepositionFeb 22, 2015-
Header (metadata) releaseMar 18, 2015-
Map releaseMar 25, 2015-
UpdateApr 15, 2015-
Current statusApr 15, 2015Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5aka
  • Surface level: 0.03
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol

Downloads & links

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Map

FileDownload / File: emd_2917.map.gz / Format: CCP4 / Size: 21.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationReconstruction of ribosome-SRP-FtsY complex in the "closed" state. The map has been b-factor sharpened. The authors report resolution of 5.7 angstrom according to FSC=0.143, which was obtained from RELION post-process (for b-factor sharpening). Since the RELION post-process does not generate new half maps, the maps uploaded for FSC validation were the half maps before b-factor sharpening.
Voxel sizeX=Y=Z: 2 Å
Density
Contour LevelBy EMDB: 0.02 / Movie #1: 0.03
Minimum - Maximum-0.09933925 - 0.15849756
Average (Standard dev.)0.00025307 (±0.01427202)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-90-90-90
Dimensions180180180
Spacing180180180
CellA=B=C: 360.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z222
M x/y/z180180180
origin x/y/z0.0000.0000.000
length x/y/z360.000360.000360.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS-90-90-90
NC/NR/NS180180180
D min/max/mean-0.0990.1580.000

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Supplemental data

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Sample components

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Entire : E. coli SRP-FtsY binds to E. coli ribosome with Lep50 nascent chain

EntireName: E. coli SRP-FtsY binds to E. coli ribosome with Lep50 nascent chain
Components
  • Sample: E. coli SRP-FtsY binds to E. coli ribosome with Lep50 nascent chain
  • Complex: E. coli 70S ribosome displaying Lep50 nascent chain
  • Protein or peptide: Signal recongnition particle and SRP receptor

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Supramolecule #1000: E. coli SRP-FtsY binds to E. coli ribosome with Lep50 nascent chain

SupramoleculeName: E. coli SRP-FtsY binds to E. coli ribosome with Lep50 nascent chain
type: sample / ID: 1000 / Number unique components: 2
Molecular weightTheoretical: 2.7 MDa

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Supramolecule #1: E. coli 70S ribosome displaying Lep50 nascent chain

SupramoleculeName: E. coli 70S ribosome displaying Lep50 nascent chain / type: complex / ID: 1 / Name.synonym: RNC
Details: Ribosome-nascent chain complexes (RNCs) were prepared by in vitro transcription and translation from pUC19StrepLep50 and purified by sucrose gradient centrifugation and affinity chromatography.
Recombinant expression: No / Ribosome-details: ribosome-prokaryote: ALL
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 2.5 MDa

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Macromolecule #1: Signal recongnition particle and SRP receptor

MacromoleculeName: Signal recongnition particle and SRP receptor / type: protein_or_peptide / ID: 1 / Name.synonym: scSRP219
Details: This is a single-chain construct comprising E.coli FtsY219 (truncated version lacking the N-terminal A-domain and the first helix of the N-domain) fused via a 31-amino acid glycine-serine- ...Details: This is a single-chain construct comprising E.coli FtsY219 (truncated version lacking the N-terminal A-domain and the first helix of the N-domain) fused via a 31-amino acid glycine-serine-rich linker to full-length Ffh.
Number of copies: 1 / Recombinant expression: Yes
Source (natural)Organism: Escherichia coli (E. coli)
Molecular weightTheoretical: 150 KDa
Recombinant expressionOrganism: Escherichia coli BL21 (bacteria) / Recombinant plasmid: pET24 and pUC19

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.5 mg/mL
BufferpH: 7.5
Details: 50mM Hepes-KOH, 100mM KOAc, 8mM Mg(OAc)2, 500ug/ml chloramphenicol
GridDetails: 300 mesh quantifoil grid type R1.2/1.3 coated with a thin continuous carbon layer, glow-discharged for 30 s
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 77 K / Instrument: FEI VITROBOT MARK IV / Method: Blot for 2 seconds before plunging

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Electron microscopy

MicroscopeFEI TITAN KRIOS
DateJan 24, 2014
Image recordingCategory: CCD / Film or detector model: FEI FALCON II (4k x 4k) / Number real images: 2840 / Average electron dose: 24 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 77769 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.6 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionDetails: per micrograph
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 5.7 Å / Resolution method: OTHER / Software - Name: Xmipp, 3.0, RELION
Details: The final reconstruction has been done in RELION auto-refine. Final map has been b-factor sharpened in RELION using an automatically calculated b-factor value of -236.9
Number images used: 32170

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Atomic model buiding 1

Initial modelPDB ID:

2aw4

SoftwareName: Chimera, Coot
DetailsThe structures were fitted by initial rigid body fitting in Chimera, followed by local refinement in Coot.
RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-5aka:
EM structure of ribosome-SRP-FtsY complex in closed state

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Atomic model buiding 2

Initial modelPDB ID:

2xxa


Chain - Chain ID: A
SoftwareName: Chimera, Coot, HHpred, CNS
DetailsThe structures were fitted by initial rigid body fitting in Chimera, followed by local refinement in Coot. The finger loop region was modelled in HHpred. The whole structure was finally energy minimization in CNS Version 1.0.
RefinementSpace: REAL / Protocol: FLEXIBLE FIT
Output model

PDB-5aka:
EM structure of ribosome-SRP-FtsY complex in closed state

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