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- EMDB-29006: human EMC:human Cav1.2 channel complex in GDN detergent at 3.1 An... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | human EMC:human Cav1.2 channel complex in GDN detergent at 3.1 Angstrom | |||||||||
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![]() | endoplasmic reticulum membrane protein complex / voltage-gated calcium channel / holdase / biogenesis / MEMBRANE PROTEIN | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
![]() | Chen Z / Mondal A / Minor DL | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural basis for Caαδ:gabapentin binding. Authors: Zhou Chen / Abhisek Mondal / Daniel L Minor / ![]() Abstract: Gabapentinoid drugs for pain and anxiety act on the Caαδ-1 and Caαδ-2 subunits of high-voltage-activated calcium channels (Ca1s and Ca2s). Here we present the cryo-EM structure of the gabapentin- ...Gabapentinoid drugs for pain and anxiety act on the Caαδ-1 and Caαδ-2 subunits of high-voltage-activated calcium channels (Ca1s and Ca2s). Here we present the cryo-EM structure of the gabapentin-bound brain and cardiac Ca1.2/Caβ/Caαδ-1 channel. The data reveal a binding pocket in the Caαδ-1 dCache1 domain that completely encapsulates gabapentin and define Caαδ isoform sequence variations that explain the gabapentin binding selectivity of Caαδ-1 and Caαδ-2. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 258.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.9 KB 16.9 KB | Display Display | ![]() |
Images | ![]() | 49.3 KB | ||
Others | ![]() ![]() | 259.5 MB 259.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 920.4 KB | Display | ![]() |
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Full document | ![]() | 920 KB | Display | |
Data in XML | ![]() | 16.8 KB | Display | |
Data in CIF | ![]() | 20 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 0.835 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_29006_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_29006_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Ternary complex of the human ER membrane protein complex (EMC) wi...
Entire | Name: Ternary complex of the human ER membrane protein complex (EMC) with human CaV alpha1C and rabbit CaV beta3 |
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Components |
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-Supramolecule #1: Ternary complex of the human ER membrane protein complex (EMC) wi...
Supramolecule | Name: Ternary complex of the human ER membrane protein complex (EMC) with human CaV alpha1C and rabbit CaV beta3 type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#11 |
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Source (natural) | Organism: ![]() |
-Supramolecule #2: Human CaV alpha1C
Supramolecule | Name: Human CaV alpha1C / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #9 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 187 KDa |
-Supramolecule #3: ER membrane protein complex subunit 1
Supramolecule | Name: ER membrane protein complex subunit 1 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 112 KDa |
-Supramolecule #4: Rabbit CaV beta3
Supramolecule | Name: Rabbit CaV beta3 / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #10 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 100 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1.7 mg/mL |
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Buffer | pH: 8 |
Grid | Model: Quantifoil R1.2/1.3 / Support film - Material: CARBON / Support film - topology: HOLEY |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 46.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.7 µm / Nominal defocus min: 0.9 µm / Nominal magnification: 105000 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: Details: 6WW7 (PDB ID) and 7MIY (PDB ID) used to generate the startup model. |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 383185 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |