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基本情報
登録情報 | データベース: EMDB / ID: EMD-2860 | |||||||||
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タイトル | Electron cryo-microscopy of dynein/dynactin/GFP-BICD2N complex | |||||||||
![]() | Reconstruction of dynein tail/dynactin/GFP-BICD2N complex. | |||||||||
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![]() | dynein / dynactin / BICD2 / motor / transport | |||||||||
機能・相同性 | ![]() COPI-independent Golgi-to-ER retrograde traffic / RHOD GTPase cycle / Factors involved in megakaryocyte development and platelet production / retrograde axonal transport of mitochondrion / Gap junction degradation / Formation of annular gap junctions / Regulation of actin dynamics for phagocytic cup formation / EPHB-mediated forward signaling / Adherens junctions interactions / VEGFA-VEGFR2 Pathway ...COPI-independent Golgi-to-ER retrograde traffic / RHOD GTPase cycle / Factors involved in megakaryocyte development and platelet production / retrograde axonal transport of mitochondrion / Gap junction degradation / Formation of annular gap junctions / Regulation of actin dynamics for phagocytic cup formation / EPHB-mediated forward signaling / Adherens junctions interactions / VEGFA-VEGFR2 Pathway / Cell-extracellular matrix interactions / RHO GTPases Activate WASPs and WAVEs / MAP2K and MAPK activation / UCH proteinases / Clathrin-mediated endocytosis / RHOF GTPase cycle / dynactin complex / Regulation of PLK1 Activity at G2/M Transition / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / transport along microtubule / visual behavior / WASH complex / F-actin capping protein complex / positive regulation of intracellular transport / dynein light chain binding / negative regulation of filopodium assembly / regulation of metaphase plate congression / dynein heavy chain binding / establishment of spindle localization / axonemal dynein complex / positive regulation of spindle assembly / ciliary tip / structural constituent of postsynaptic actin cytoskeleton / dense body / Intraflagellar transport / dynein complex / Neutrophil degranulation / COPI-independent Golgi-to-ER retrograde traffic / P-body assembly / coronary vasculature development / minus-end-directed microtubule motor activity / barbed-end actin filament capping / dynein light intermediate chain binding / cytoplasmic dynein complex / regulation of cell morphogenesis / retrograde axonal transport / RHO GTPases activate IQGAPs / regulation of lamellipodium assembly / RHO GTPases Activate Formins / aorta development / nuclear migration / Recruitment of NuMA to mitotic centrosomes / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / COPI-mediated anterograde transport / ventricular septum development / centrosome localization / microtubule motor activity / dynein intermediate chain binding / dynein complex binding / NuA4 histone acetyltransferase complex / microtubule-based movement / cytoplasmic microtubule / Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal / COPI-mediated anterograde transport / stress granule assembly / stress fiber / Mitotic Prometaphase / cytoplasmic microtubule organization / EML4 and NUDC in mitotic spindle formation / regulation of mitotic spindle organization / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / axon cytoplasm / Recruitment of mitotic centrosome proteins and complexes / cytoskeleton organization / Recruitment of NuMA to mitotic centrosomes / Resolution of Sister Chromatid Cohesion / Anchoring of the basal body to the plasma membrane / HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / MHC class II antigen presentation / sarcomere / AURKA Activation by TPX2 / axonogenesis / cellular response to nerve growth factor stimulus / mitotic spindle organization / filopodium / RHO GTPases Activate Formins / cell motility / actin filament / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / cell morphogenesis / kinetochore / cilium / Aggrephagy / microtubule cytoskeleton organization 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 8.2 Å | |||||||||
![]() | Urnavicius L / Zhang K / Diamant AG / Motz C / Schlager MA / Yu M / Patel NA / Robinson CV / Carter AP | |||||||||
![]() | ![]() タイトル: The structure of the dynactin complex and its interaction with dynein. 著者: Linas Urnavicius / Kai Zhang / Aristides G Diamant / Carina Motz / Max A Schlager / Minmin Yu / Nisha A Patel / Carol V Robinson / Andrew P Carter / ![]() 要旨: Dynactin is an essential cofactor for the microtubule motor cytoplasmic dynein-1. We report the structure of the 23-subunit dynactin complex by cryo-electron microscopy to 4.0 angstroms. Our ...Dynactin is an essential cofactor for the microtubule motor cytoplasmic dynein-1. We report the structure of the 23-subunit dynactin complex by cryo-electron microscopy to 4.0 angstroms. Our reconstruction reveals how dynactin is built around a filament containing eight copies of the actin-related protein Arp1 and one of β-actin. The filament is capped at each end by distinct protein complexes, and its length is defined by elongated peptides that emerge from the α-helical shoulder domain. A further 8.2 angstrom structure of the complex between dynein, dynactin, and the motility-inducing cargo adaptor Bicaudal-D2 shows how the translational symmetry of the dynein tail matches that of the dynactin filament. The Bicaudal-D2 coiled coil runs between dynein and dynactin to stabilize the mutually dependent interactions between all three components. | |||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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ダウンロードとリンク
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マップデータ | ![]() | 10.9 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 10.8 KB 10.8 KB | 表示 表示 | ![]() |
画像 | ![]() | 2.2 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 200.8 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 199.9 KB | 表示 | |
XML形式データ | ![]() | 7.3 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 5afuMC ![]() 6f3aM ![]() 6zo4M ![]() 2854C ![]() 2855C ![]() 2856C ![]() 2857C ![]() 2861C ![]() 2862C ![]() 5adxC ![]() 5afrC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | Reconstruction of dynein tail/dynactin/GFP-BICD2N complex. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.34 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
-全体 : Cryo-EM structure of dynein tail/dynactin/GFP-BICD2N
全体 | 名称: Cryo-EM structure of dynein tail/dynactin/GFP-BICD2N |
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要素 |
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-超分子 #1000: Cryo-EM structure of dynein tail/dynactin/GFP-BICD2N
超分子 | 名称: Cryo-EM structure of dynein tail/dynactin/GFP-BICD2N タイプ: sample / ID: 1000 / 詳細: The sample was monodisperse / Number unique components: 3 |
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分子量 | 理論値: 2.6 MDa |
-分子 #1: Human cytoplasmic dynein 1 tail
分子 | 名称: Human cytoplasmic dynein 1 tail / タイプ: protein_or_peptide / ID: 1 / Name.synonym: TDB / コピー数: 1 / 組換発現: No |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 2.6 MDa |
-分子 #2: mouse BICD2N
分子 | 名称: mouse BICD2N / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 組換発現: No |
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由来(天然) | 生物種: ![]() ![]() |
-分子 #3: pig dynactin
分子 | 名称: pig dynactin / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 組換発現: No |
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由来(天然) | 生物種: ![]() ![]() |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 0.075 mg/mL |
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緩衝液 | pH: 7.4 詳細: 150mM KCl, 25mM HEPES-KOH, 1mM MgCl2, 0.1mM MgATP, 5mM DTT |
グリッド | 詳細: Quantifoil R1.2/1.3 400 mesh copper grid with thin carbon support, glow discharged |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 120 K / 装置: FEI VITROBOT MARK III / 手法: Blot for 3.5 seconds before plunging |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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温度 | 平均: 100 K |
アライメント法 | Legacy - 非点収差: Objective lens astigmatism was corrected at 120,000 times magnification. |
日付 | 2014年7月17日 |
撮影 | カテゴリ: CCD フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 実像数: 4259 / 平均電子線量: 1 e/Å2 / ビット/ピクセル: 16 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 倍率(補正後): 82353 / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 8.0 µm / 最小 デフォーカス(公称値): 3.0 µm / 倍率(公称値): 47000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
詳細 | The particles were selected using an automatic selection program. |
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CTF補正 | 詳細: Each particle |
最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / アルゴリズム: OTHER / 解像度のタイプ: BY AUTHOR / 解像度: 8.2 Å / 解像度の算出法: OTHER / ソフトウェア - 名称: Relion / 使用した粒子像数: 85744 |