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- EMDB-23926: The structure of the Tetrahymena thermophila outer dynein arm on ... -

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Basic information

Entry
Database: EMDB / ID: EMD-23926
TitleThe structure of the Tetrahymena thermophila outer dynein arm on doublet microtubule
Map data
Sample
  • Organelle or cellular component: Outer dynein arm complex from Tetrahymena thermophila
    • Protein or peptide: x 22 types
  • Ligand: x 5 types
Keywordscilia / doublet / axoneme / outer dynein arm / dynein / STRUCTURAL PROTEIN
Function / homology
Function and homology information


outer dynein arm / outer dynein arm assembly / dynein light chain binding / cilium movement / dynein heavy chain binding / axonemal dynein complex / dynein complex / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / cytoplasmic dynein complex ...outer dynein arm / outer dynein arm assembly / dynein light chain binding / cilium movement / dynein heavy chain binding / axonemal dynein complex / dynein complex / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / cytoplasmic dynein complex / motile cilium / dynein intermediate chain binding / microtubule-based movement / microtubule-based process / protein-disulfide reductase activity / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / structural constituent of cytoskeleton / cilium / microtubule cytoskeleton organization / mitotic cell cycle / microtubule / hydrolase activity / GTPase activity / centrosome / GTP binding / ATP hydrolysis activity / ATP binding / metal ion binding / cytoplasm
Similarity search - Function
Outer dynein arm-docking complex subunit 3 / Kelch motif / Galactose oxidase, central domain / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Dynein light chain type 1 / Dynein light chain, type 1/2 / Dynein light chain superfamily / Dynein light chain type 1 ...Outer dynein arm-docking complex subunit 3 / Kelch motif / Galactose oxidase, central domain / Dynein light chain Tctex-1 like / Tctex-1-like superfamily / Tctex-1 family / Dynein light chain type 1 / Dynein light chain, type 1/2 / Dynein light chain superfamily / Dynein light chain type 1 / Dynein heavy chain, AAA 5 extension domain / Dynein heavy chain AAA lid domain / Roadblock/LAMTOR2 domain / Roadblock/LC7 domain / Roadblock/LC7 domain / Dynein heavy chain 3, AAA+ lid domain / AAA+ lid domain / Dynein heavy chain, C-terminal domain / Dynein heavy chain, C-terminal domain, barrel region / Dynein heavy chain C-terminal domain / Filamin/ABP280 repeat / Filamin/ABP280 repeat profile. / Filamin/ABP280 repeat-like / P-loop containing dynein motor region / Dynein heavy chain, tail / Dynein heavy chain, N-terminal region 1 / Dynein heavy chain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, linker / Dynein heavy chain, AAA module D4 / Dynein heavy chain, coiled coil stalk / Dynein heavy chain, hydrolytic ATP-binding dynein motor region / Dynein heavy chain, ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Dynein heavy chain AAA lid domain superfamily / Dynein heavy chain, domain 2, N-terminal / Dynein heavy chain, linker, subdomain 3 / Dynein heavy chain, AAA1 domain, small subdomain / Dynein heavy chain region D6 P-loop domain / Dynein heavy chain, N-terminal region 2 / Hydrolytic ATP binding site of dynein motor region / Microtubule-binding stalk of dynein motor / P-loop containing dynein motor region D4 / ATP-binding dynein motor region / Dynein heavy chain AAA lid domain / Thioredoxin / IPT/TIG domain / Kelch-type beta propeller / ig-like, plexins, transcription factors / Thioredoxin / IPT domain / Thioredoxin, conserved site / Thioredoxin family active site. / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Thioredoxin domain profile. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Thioredoxin domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / Immunoglobulin E-set / Thioredoxin-like superfamily / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Immunoglobulin-like fold / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Dynein light chain tctex-type 1 protein / Dynein intermediate chain 2 / EF hand protein / Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative / Outer arm dynein beta heavy chain / Dynein light chain roadblock-type 2 protein / Tubulin alpha chain / Tubulin beta chain / Dynein light chain / Dynein light chain ...Dynein light chain tctex-type 1 protein / Dynein intermediate chain 2 / EF hand protein / Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative / Outer arm dynein beta heavy chain / Dynein light chain roadblock-type 2 protein / Tubulin alpha chain / Tubulin beta chain / Dynein light chain / Dynein light chain / Dynein light chain / Dynein light chain / Thioredoxin / Dynein light chain 2A / Dynein heavy chain, outer arm protein / Dynein light chain roadblock-type 2 protein / Dynein light chain / Uncharacterized protein / Outer dynein arm docking complex protein oda protein / Flagellar outer dynein arm intermediate protein, putative / Dynein light chain
Similarity search - Component
Biological speciesTetrahymena thermophila CU428 (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 8.0 Å
AuthorsKubo S / Yang SK / Ichikawa M / Bui KH
Funding support Canada, 2 items
OrganizationGrant numberCountry
Natural Sciences and Engineering Research Council (NSERC, Canada)RGPIN-2016-04954 Canada
Canadian Institutes of Health Research (CIHR)PJT-156354 Canada
CitationJournal: EMBO Rep / Year: 2021
Title: Remodeling and activation mechanisms of outer arm dyneins revealed by cryo-EM.
Authors: Shintaroh Kubo / Shun Kai Yang / Corbin S Black / Daniel Dai / Melissa Valente-Paterno / Jacek Gaertig / Muneyoshi Ichikawa / Khanh Huy Bui /
Abstract: Cilia are thin microtubule-based protrusions of eukaryotic cells. The swimming of ciliated protists and sperm cells is propelled by the beating of cilia. Cilia propagate the flow of mucus in the ...Cilia are thin microtubule-based protrusions of eukaryotic cells. The swimming of ciliated protists and sperm cells is propelled by the beating of cilia. Cilia propagate the flow of mucus in the trachea and protect the human body from viral infections. The main force generators of ciliary beating are the outer dynein arms (ODAs) which attach to the doublet microtubules. The bending of cilia is driven by the ODAs' conformational changes caused by ATP hydrolysis. Here, we report the native ODA complex structure attaching to the doublet microtubule by cryo-electron microscopy. The structure reveals how the ODA complex is attached to the doublet microtubule via the docking complex in its native state. Combined with coarse-grained molecular dynamic simulations, we present a model of how the attachment of the ODA to the doublet microtubule induces remodeling and activation of the ODA complex.
History
DepositionMay 3, 2021-
Header (metadata) releaseJul 14, 2021-
Map releaseJul 14, 2021-
UpdateMay 29, 2024-
Current statusMay 29, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 3.1
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 3.1
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7moq
  • Surface level: 3.1
  • Imaged by UCSF Chimera
  • Download
  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-7moq
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_23926.png

Downloads & links

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Map

FileDownload / File: emd_23926.map.gz / Format: CCP4 / Size: 6.3 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 2.73 Å
Density
Contour LevelBy AUTHOR: 3.1 / Movie #1: 3.1
Minimum - Maximum-5.3616934 - 14.861065999999999
Average (Standard dev.)0.6256219 (±2.3120282)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin7110073
Dimensions85169115
Spacing11585169
CellA: 313.95 Å / B: 232.05 Å / C: 461.37 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.732.732.73
M x/y/z11585169
origin x/y/z0.0000.0000.000
length x/y/z313.950232.050461.370
α/β/γ90.00090.00090.000
start NX/NY/NZ7371100
NX/NY/NZ11585169
MAP C/R/S321
start NC/NR/NS1007173
NC/NR/NS16985115
D min/max/mean-5.36214.8610.626

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Supplemental data

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Sample components

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Entire : Outer dynein arm complex from Tetrahymena thermophila

EntireName: Outer dynein arm complex from Tetrahymena thermophila
Components
  • Organelle or cellular component: Outer dynein arm complex from Tetrahymena thermophila
    • Protein or peptide: Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative
    • Protein or peptide: Outer arm dynein beta heavy chain
    • Protein or peptide: Dynein heavy chain, outer arm protein
    • Protein or peptide: Dynein intermediate chain 2
    • Protein or peptide: Flagellar outer dynein arm intermediate protein, putative
    • Protein or peptide: Dynein light chain roadblock-type 2 protein
    • Protein or peptide: Dynein light chain roadblock-type 2 protein
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain
    • Protein or peptide: Dynein light chain 2A
    • Protein or peptide: Dynein light chain tctex-type 1 protein
    • Protein or peptide: Thioredoxin
    • Protein or peptide: Tubulin beta chain
    • Protein or peptide: Tubulin alpha chain
    • Protein or peptide: Outer dynein arm docking complex protein oda protein
    • Protein or peptide: Docking complex 1/2 protein
    • Protein or peptide: Docking complex 1 protein
    • Protein or peptide: Outer dynein arm docking complex protein oda protein
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: GUANOSINE-5'-DIPHOSPHATE
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION

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Supramolecule #1: Outer dynein arm complex from Tetrahymena thermophila

SupramoleculeName: Outer dynein arm complex from Tetrahymena thermophila / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1-#22
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428 / Organelle: cilia / Location in cell: cilia
Molecular weightTheoretical: 2 MDa

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Macromolecule #1: Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protei...

MacromoleculeName: Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 475.554406 KDa
SequenceString: MPQPLVWTQL KQTGTTQPTA RSGHTIITVG KTHIMFGGLD NDKNNYKDGK IAPNNQVFTL KLTQNNCEWR QIACQGDVPL PRCYHASCA ISADKMLVFG GSYTSNLRFN DTYILKTTSY QWSKPANQIS GGEPKNAESK IGAPQPRYGH SATFFEGKVY I FGGHGGIN ...String:
MPQPLVWTQL KQTGTTQPTA RSGHTIITVG KTHIMFGGLD NDKNNYKDGK IAPNNQVFTL KLTQNNCEWR QIACQGDVPL PRCYHASCA ISADKMLVFG GSYTSNLRFN DTYILKTTSY QWSKPANQIS GGEPKNAESK IGAPQPRYGH SATFFEGKVY I FGGHGGIN YQRLAFNDLY VLETENFEWT RLEPKGNPPD PRGGHSAAMM ANKPQLMIFG GWSFTSQYSN IMIYDIEKDE WV DPEIAHE IPKWNLSGIM APSIPSWKYF IFGGSVGSFE EGGNRTNSRF VDDSFVLDID TLSWSSINLE ADETSKAVCK PRP RESASI FYDSGESRAI VFGGWANNWL NDLWALNVST ITGPPYAIFS IKPALGPLTG KTKVLIEGDG FKDTQNISVK FSGG KLEKE VNGTFVNEKE ISCETPTFDY PRSVEVTVCM NKGDYTITKS AFTYYLNTKA DKTIAYGPGL LTENLIGVQT TIVIQ ARNK NDQNRESGSD EFVVTIRNPA KIKKEEEVKE GDKANTKNTI KEDEEEEGED EEENKKKKEA EKAAAEKAAA EKAAEE EQG EAVDPSLVPF NIVDNDDGSY FIQFTSEEEA VLEIDIKFKD ENNELHSIRG NPFRCGFVKG SKPNNNDLTG SAVMNYI SK QLKDIQEFIE NTKENIEIRN KNIRENVSEL INVMINLEKV RVKNDDDVLT LDTVEEMLNF LKKKDFGKDS DIKKCKKL Q EEWKNLAKMA QAVKKDIQNP VKTESDKTKE NIKKFEEITL KEYANSLKKE SFFIYKTGVS ESFKRIGEVK QKVDEFEVQ LNQYEDFARM FEFPDAVIGS KKLMEQIRTD VSSVEKLWVR IEISEKTMDE YKKMKWGSIN SMDMEDEIKK LRKALTDLRG IDKRSNAFI GITEELKKWA TFLPLLGELK DPSMNSEDGR HWKKLKDLVK KEFDVSQELM LEIIWDLKLF DYKDGIEEIT D QAKQELKM EKALKKIIDF WRDIEFELVQ HKNTDIHTLK MSEENFETLE DHQLQINNML LSKYVAYFEK EVEKWKYDLG SV YDVVQLL LEVQKTWSFL ENLFIQSEEV KRELPNESAQ FVGIDKDMKE IMQKGCDIKN CLKFCTIEGM LKRLENIQAQ LKV CEKALN EFLDSKRRAF PRFYFVSVND LLDILSNGNS PAKINRHMSK IFQAIDNLQL KEDSSGGRPT ALKMISCVGT EEVD FSSPR LLQGKVESYL KDVIDTMIGT LKSVANSSFK NFQSMTRKEW LKSDPSQITL LVNNIIWSKA VEDCFLKLQS GDINA MKLF LDESIKQLTE LIGMVQGDLS KPLRQKIMCL ITIDTHSRDV VHRLINEHVR KAEEFQWQSQ LKFYWVDNDA KIKIAD ARF VYNYEYLGNG PRLVITPLTD RIYVTATQAL HLKMGCAPAG PAGTGKTETT KDLANALAKA CYVFNCSSEM NYESMGN IY KGLASSGCWG CFDEFNRLLP EVLSVCSVQF KAVTDAIKQN VERFIIEGDE ISLDPTCGVF ITMNPGYLGR AELPEGLK A LFRPITVVVP DLELICENML MAEGFIEAKI LAKKFVTLYM LCRDLLSKQL HYDWGLRAIK SVLVVAGGFK RSEPEIAEQ ALLMRALRDF NIPKIAFQDL YVFHGLLGDL FPGINIKPKK DLDFEKIITD VCIENKLDPD PEFVLKVVQL SELLAIRHCV FVMGPPGAG KSTTWKILAK AQDKTNKKTT LIDIDPKVVS TKDFYGYNLP SKEWKDGLFS KMLRSLAEQP DTNPKWICLD G DLDANWIE SMNSVMDDNK ILTLANNERI PLKPHMRALF EIRDLRFATP ATVSRAGILY ISDEVGYQWR SYVKSWIKQE FS QDQEMSK NLDTLFGKYV PDTLDHIKKH CRFLVPVSPI SQVISICKSL QTLLKGDVKN LEYLFVYALI WAIGGALAEK DSI DYRKDF STWWKGAWKT AVKFPSKGTI FDYYVDQSGD SSKFVEWSKR LENKEFDPQV ETMGNITVNT IETLATTEFI KSYL MVKHP SLLIGNSGCG KTQLAKGILK EIVQAKPENY AYQLINFNYY TDSTYLQGQI EQTLEKKAGR QYGPPGKVQL IYFID DLNM PQLDAYDTQT AIALLRQLAD YGHFYDVSKL ALKDIINTQV LAAMNPSAGS FFVNPRYQRH FWTISIPFPD NESLSL IYI TFLNGHLKRF KSTIQEYSNI IVRASLMLHQ AVTQNFRKTA INFHYEFNLR HMSNVFQGLL LSDPNKFTEP DKLIKLW IH ECERTYGDRL VSTDNLKTYK ENIFDIVKKS FSKFNFSRYF GNNPENLIYC NFIAGINSDR FYDQMPNNEM EKHISEAL K EYNDNNAFMG LVLFEDAMKH VCRICRIVLP SSGHALLVGV GGSGKQSLSK LASFIMGYTT FSITISATYS MVDLRNDLQ QLYFKCGPKE EGILFLFTEG QITNERFLVY INDLLSSGEI AELYTLDEKE AMINQVRAKV KGEGKPDTRE NCWNWFIDQV KKNLHMAIC FSPVGDMRRR ARQFPALVNC TVIDWFQPWP YEALFNVAKS FLEPVDLGDD KVREAVVKFM PFSFTLVNDL G LKLLEQER RYAYTTPKSF LELISLFTNM LAQKRESLER NKERYETGLV KLKETAEQVA IIEVEVKEKQ VEAEAKKKEA DA FAEVVGR EKDKVEKENS KATIEADKCG LIKQNVEAQK SSTQQDLDAA QPLVEQAKSA LNSISKKDFQ QAKSFASPPA GVP EVFAAT IYLLAGYFNE AIEIDKNKKP KDVSWKSSLK LMKSPEEFME KLLNFKDVVD ANQVPAANVN IVKNQYLNMP SFTP EQMAS KSAAAKGICS WVVNIVKYYD VIQDVEPKRK ALKEATEQLE EATVKLNEVE EVVRKLNEEL NKLKAENDKA IAERN AAIS EAERCARRLN LAQRLVTALS SENERWGKSI IQLEDQLKLM VGDVLVASSF VSYSGPFNKK FRNIMINQNF MKFMKE HTI PMSPDPNPIK ILTDESTIAL WNKQKLPSDS VSIENGTILT NSARYPLMID PQLQGITWIR EKEKANNLKI LRLGSKN IN RDLELSIENG YSAIIENMNE RIDAILMPII ARSFIKRGKN KIIKFAGKDL ILHPNFKLFL HTKLSNPHYP PEIQAEAA L INFTVTEAGL GDQLLSLVVA RERPDLAKMK IELITQQNDF KIKLKDLEDE LLYKLANAKG DILDDIELIE NLEYSKKLS VEIAEKVAAA KITEAKINET SENYRPAASR GALFYFLLSD LSKVHSFYKY SLESFIVVIN RAIDAISENK IYGKTTMVPY GDETYASNQ HEKEEEEEEE EHQQAQQQQN QEEQQEQKDE ENKGPVEEEQ PEGENKGPVE EEGAEGQNEG PVEEENAENQ E GEGEEEGE KQQKAKDSDE PMSPRSLKKR VDELIESLTY TAYQTTRRGL FESHKLIVAA MLCLRVLLRS EELNSDEVDH LI IGKVDVN PTPMPDALKS FLNDNIWAAC KALETIHQFQ GFCQSLETDV LQWKKWYSEE KAETADLPKA FKELSKFHRL LLL RALRPD RLPSALSQFV HDKMGERYIE QPPFNIFETF QETSKTVPIF FVLFPGVDPT PDVERVAATF DVSANNGRFI NISM GQGQE DRAKKALFDC AQKGHWIMLQ NVHLMQSWLY GLNGLEGFLE SVFASPKTHP NFRVFISSEP PNVLLPLMQI IPESI LQGS LKIANEAPQY LKANLRRAYN KFDQEFLDKC DKKPTEFKSC LFALCFFHSL MLGRKKFGTQ GWSRVYNFND GDLTIC ADV LYNYLSKYDQ VPWDDLRYIF GEIMYGGHIT DDWDRRTNRT YLKVLIRSEL LQQNFNLAPQ FKSPDPSKFD YEAYRKY IE EKLPIESPQM FGMHPNAEIG YLTQTCDQVF NTILEVQGGS SGGGASKKDD GVMVTLTDFK TRCPHDFNML LIEEKVKE K TPYIVVCLQE CERMNGLLKE IKTSLEDLRL GLTGALNMTD AMESLQQSLS FNKVPDTWEK KAYFSKKPLS SWFADLIER NIQLQEWCKE LVTPTSLCIS YLFNPMSYLT AIMQFTARAQ GLPLDGITIQ TNVTAMKGPE DVVNPAENGA YIHGLFLEGA AWEIGGQGQ DGYLIEQKPK ELHPKMPVIN AVAVPLDKKK KNGQYDCPTY VTSARGQTFV FTANLNMESD DSDPNKWILS G TCMLMSDD

UniProtKB: Dynein-1-alpha heavy chain, flagellar inner arm I1 complex protein, putative

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Macromolecule #2: Outer arm dynein beta heavy chain

MacromoleculeName: Outer arm dynein beta heavy chain / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: SB715
Molecular weightTheoretical: 530.111312 KDa
SequenceString: MGDHSQKDSP EDFIINRLSQ ALGIQKEKIK KSLETQQDDK GEVTNKDEFQ GFIQQDNSTN ILWVSGQSEK CTFYYGQLPP IDKFKKKGI AVIKLGLHKL TNENVAKDVV VVEITNNLLE HLNSVFNEIM SPVMQNPLNQ QGWTDLVAKD LMEKFNNYVA Q VYVLLGQI ...String:
MGDHSQKDSP EDFIINRLSQ ALGIQKEKIK KSLETQQDDK GEVTNKDEFQ GFIQQDNSTN ILWVSGQSEK CTFYYGQLPP IDKFKKKGI AVIKLGLHKL TNENVAKDVV VVEITNNLLE HLNSVFNEIM SPVMQNPLNQ QGWTDLVAKD LMEKFNNYVA Q VYVLLGQI KGKTMLPLPS HKLTSSDTTP DKDKAHVFEG SIITWTKQIK NVLKLEPEQL LKYGNDPGPL AEIEFWQNKR DN LNLIDSQ LKSVEVQNIL HFLDNNKSTY TTPFTKLQAE VKKARLEANE NYRYLFTLKD LFSKLQESQP SDFPTLYELF IPI MHTILL IYNKSKTYNQ PPRLVVLIRE ICNAIISNAQ AFVDKDTIFS LIDSKETTEA CDKLQVTLDV CSKFKDAYFE YKAK AGGNW KLTSNALFVR LDSFLERCQD ILHLTNTIVQ FNKLEKIELG GTKGKTLTES IAQIFKEFEE AVQAFTSVSY DIMNI AEKK FDDDFYEFRS KIKELERRLA SVITQGFDDY DTIYGRFKLL DNFEGLLTRP IIADELEKKH IVLLEMYKQD LKQVQS IFL EGKQFVDSMH ENAPLFLNMP PIAGALTWCK SLRDRIQEPI EKLAQLGQGI TEREEYKDVQ KLYTSITKSI KDYEDQK IL SWEKEVEDSS QDKLKQTLLC KDENDLIKVN FDPSLVRLLK EVKYFLLLRL EVPTTAKDIY TKAETYRTQI VALDMIVD N YNHIKTCLLP VEEPLVKKKI QDMEEEVKPG IEEIRWKSTN IDQFISKSKS IVDQLFETVN KMKDSLQKIH KSLANFNVK IIERKNRPMS PDDYDQFLKA IFSNKLTIVK DNGNQIQKLV KEVLDAVKAD KKQNSWKNYN DYVNVIVIEG ISTAIQTALL HLNEQINPV FIKRNDISPL FDIRLELGQS GIQFDPEIGE SSNQLTVRNT IRNWINDFFN IAGTIQRLDT TMPGDFLQEI R SFFEIKQC LAMITQNLEW IENECNQFRA RFDTYSYLWT EDEQISFNRF LDENEPKDED GKGGDDDEGE NTEKQNPLLK GC RAKIPNL DLFDEKITHL KAIQQEISRI KTPEDISWLR INLQPMKTAL DARVTRWIRV YTDFLVNQFR TTQKNLLDFI EKT KDGIKK NPADHENLHD KKLLMSVMKV ISDVKDVEPR REGIITRMKE MVTKLKKHNV PITEKGTDDP LQQIDNANSN FIEI YGRVF KVKADIIPLQ AEETQNIKRD LDIFMKEVES FRKEFMQKLP FDYTESMGYE NINNAYDTIM VYYHKLTAIE GRALE YNNL EKLFELQKSN YKQLKDCMND LKNLKTMWDA IALIHFQYND WKTKPWRQIK ADILLDTNKT LGTQIKNLPK EIRNFK GYN VIVEKVKNMG TVLPLVSALH SEFMEDRHWS QLKQITGTVF DHNSLSFYFE DILALNLYKY ENTVNEIVDV AQKEAKI EK KLKNIEQWWS KQVFEFTEYK ETKTFASLDN MMEVLDQHSL DLMGMKSQGK YVEFFYDRVE DWREKLGRVD VVVNEWLK V QKNWKILYNI FLLSEDIRMQ LPEDTKVFEG VDKEFKDMMS EVSANPSVVE ACTIERRDVL VGWSQAIKKC EKALNDYLE QKKKSFPRFY FLSNQSLLTI LSNGQNAPKV CEYLGDCFDG LKTLTFEPPA NPAETSKVGI GMISKDDEKV PFSSKFICEG AVEHWLLNL EFRMRETLQE ILEGAKNTAD LWDSGDKPRE EWVEGYNAQI ALLTTTIVWT EDVGRAFEDL AGGSETAMKE C QKLIEVRL ENLIKKVRGD LHILERWKII NIITIDVHSR DVVEKFVIQK VSEAESFAWL SQLKFYWENK PDSDMHLRQT LR FPWEKDK NKNKCIIRIV DWFRFYSYEY IGNAIRLVIT PLTDRCYITL TQALNLTMGG APAGPAGTGK TETTKDLGRA IGI PVMVFN CSDQMNKDSM AQIFMGLSQS GAWGCFDEFN RISIEVLSVV STQVKCVLDA LKEKKTKFSF VEEGEIQLQD TVGF FITMN PGYAGRTELP ENLKALFRSC AMVVPDLALI CENMLMSEGF TMARVLSRKF VSLYMLSREL LSKQKHYDWG LRAVK SVLR QAGKLKRGDP DMPEDPLLMR ALRDFNMPKI VTDDKVIFRR LIGDLFPKLD PPTKQNPELK KIVQDTTKKD MGLVAE ELF VTKVVQLAEI LEVRHCCFVI GPPGSGKTCV WKTLIKSYIN SGEDAEYDTL NPKAVTSDEL FGAYTKTKEW KNGVIAV IM KNQVKNEEKY KATHMHKWSV LDGDIDPEWI ESLNTVMDDN KVLTLVSNDR IFLTPQMRLI FEISNLRNAT PATVSRAG V LFINETDIGW MPYMNSWLER SQINILKQQK EMANMPEYPV IDDVAKSVFY RCFQSYFEQN IDVHDKNRVR HICPMVDIA MIQTICTILD ALLIQHLPKL KQMKEEDEKQ ALEAFFIFAG LWAIGGPVGG GQDDSKDMKE FNTVWKGAAK VKFPEQGLCY DYYYDINEN KWNTWKVEDY LPNDQPLFSK IYVATIHTTR LRYMIDIHLQ RRKPILFIGS AGTGKTAVVR DYLNSTRPEQ V SHKTINFS SFTDSLALQK NIESMVEKKN GRNYGSATNK VLICFIDDFN MPYVDKYGTQ SPIQLLRLIL DYGSIFNREQ LE ERKFLQD LLFFGCLNQK SGSFTVDLRL QRNFSVFSMY TPSSDVIKTI FGSILNAHLS TIDDKAQKMA FKLVEATYFT FDK ILKNTT AFPSAKRFHY QFNFRELARV CEGICRTTPG QYSGGDQGKL VRLWAHEMKR TFEDRFIANE HVEFFRRYLT EAIS KCIGE FPETENPIAE PLIFTGFVAA HQGLDQQYTQ CTIPVLKRVL DDKLEEYNEV KAQMNLVLFQ QAMEHVSRIC RILDM PGNN ALLVGVGGSG KQSLCRLSTF INGFEIDQLV VTASFTINDL RNNLQEIYKK IAKPNSIARV FMITDSQIKE EQFLIP IND MLNSGWIFDL FPKEDMDSLV SGVRNEAKGE GVDVNNLTAL TSYFLDKIRK NLKVVLCFSP VGDTMRIRSR KFPGIIN NT SIDWFHPWPH EALIDVAFRF LEEIEFPTEE IRQSISLNMA KVHSSIDTAN EKFLKLERRY NYTTPKSFLE LIDFYKKL L TEKRETIQRQ IQRYEMGLNI LAETQNKVQG LQEELKVKMV EVNKQREETD ILIEKVGKES ALAEEEQTIA NAEEEKTNV AAAEAEKISK EATEALAEAL PALRSAEAAV DCLKKPHVTE MKNLGSPPAG VIVTARVVLI LFNQGITLND PDEKVWKKAV TFMNNPQAF IDKVKSFDGE NIEPNIIEQS NKIIQDPSKK FNEKDMAGQS YAASKLCAWA VNIVTFNKIF KQVKPLQDAQ K QANEILEE KKKELAIVKQ RVAELNARVN SLKRQLEEAE ARKMIVEQDA ARCQSRLSAA ENLVNGLAGE NKRWTQNVKF LK ENIKSMI GDSLLASAFV SYIGAFSAKL RLELWKNTWL PDIIEKGIPI TEGIEPLKIL TTEAIKSKWK NEGLPADPMS LEN AAIITA CARWPLIIDP QLQGSTWIRG KQGENLTTIS LSQPKWLGAL TSSISSGRAV LIEGIQQEID ATLDPLLQRA VKKN GNQLQ LEIGGDPIDY DPNFKLFLMT KLINPHFRPE IAAQCTIINF IVTESGLEEQ FIAMVVNIEK NELEMAKQDL VKKQN EYAV TLDKLESDLL QSLSEADPAT ILDNTELIQN LDKTKKTTIE ITEQQQKAKV TEAEINIQRE HYRVVAAEGS MLYFLV ISL SVMDHMYQYS LESFITFFFK AINRTTVRDE NRIPTLILNI RQTIYQWISR GLFEKHKLIF LTLIVFRLMQ KKIIDVA YE VAEMDFLIKC PARPGVENTL DWLPNISWDQ IQGLINLEEF RNFAHQLEKE APNRFKDWYN ELQPEDQKLP LDWKRLDS M PFKKLLVLRC LRPDRMTISL NNFIRAVLPQ GDAFVEMDQK LAFSEILESV INEDSESTIP IFFILSPGSD PVKEVEKIA KKKRIEPGKN FFNIALGQGQ DEIARRRIEE GNKEGHWVML QNIHLMPTWL IELEKILDSY SGEAGGGNSE FRLFLSAEPS TGIPIGILD RSIKLTNEPP AGLKANMKRA WTYFSKEEIE DKDPKIKSIL FALCFFHSTL IERRRFGPKG WNMSYPFNMG D LRDSYLVM NRYMEQNQGG KVPFNDLIYI FGEIMYGGHI VDDWDRRLCN SYLFNTMHEQ LFDELELFPY IEGKGLSFKV PG QNPYEKY IEHIETSLKQ ETPLAYGLHP NAEIGFRTDQ CKTLFNTLLE LMPKEQSRDE KSSDIKSSNE MASDLIKQLL EDS ELKNKI FNMEEIKNKI DAENKGPYQN VFLQEIEYMN ALLSEIVKDL EEIGQGLSGL LTVSENMEMI IESIALSRVP ASWQ KLAYP SKRGLQSWLA NLFQRIEQLN IFRDDPYSIP RVVMISRFFN PQSFLTAIMQ VISRAKAYEL NKLYIQTEIT KRSIE EIEG AAKEGAYVYG FILEGARWDY QLGQLEESKP KEMFSVLPVT YCKAIPLPPE GKEDKSLYQC PVYKTEDRGN TYVFTA QLK TRFPPRKWIL AGVAIIMDVE GVSDEVKKDK K

UniProtKB: Outer arm dynein beta heavy chain

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Macromolecule #3: Dynein heavy chain, outer arm protein

MacromoleculeName: Dynein heavy chain, outer arm protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 534.328812 KDa
SequenceString: MLSSKYSKRI AWMKTTICDS LQLKDMIVEE SFQYEKNKNL LEQFLSGEGL NKIFAYYQVQ EQAQNDDIKD TGAQDPVLFF TTGDLEKIQ DKAVWFLRIT NPADDKKKAS QQDGNDNDII FGEITPNTVP MLNALMESVY SRQIDHIITE KIQFWGVAEE E QVLEFQQH ...String:
MLSSKYSKRI AWMKTTICDS LQLKDMIVEE SFQYEKNKNL LEQFLSGEGL NKIFAYYQVQ EQAQNDDIKD TGAQDPVLFF TTGDLEKIQ DKAVWFLRIT NPADDKKKAS QQDGNDNDII FGEITPNTVP MLNALMESVY SRQIDHIITE KIQFWGVAEE E QVLEFQQH SNKFSSEVRE AINLMSPGTE HFKLDYEAIS GLSESEKMQH YEMKFNEWIN LISSQLNDDS EVRKDEKDAG PA TELIYWR SRMQKITNWS EQLKSKDFQI VKASLQRHKN HDNQRPRGDE SLSKLMMEYN RLDLLLTDKL NEAKDNVKYL TTL EKFIEP LYNGTPQQII DTLPALMNAI KMIHTIARFY NTTDKMTGLF IKITNQMIKN CKDRILNKKD NGDNPSLYKM IWEQ DPAEL IEVLGSCIKL YCEYKKCYND TKEKVADMPK GKTFDFSDAQ IFGKFDTFVR RLQKLIEIFS NIQQFNALAK HNLEG MDVL TNKFKKIIDD FKKKGHNLLD TANNKFDRDW VEFNVEISHL DGELQNFIDN NFNRFRNIEY SLKLLHKFQS TIKRDS LKH NLTSRYNAIL HNYATELDTI QRVFQDQKSN PPLVRNMPPE AGKIIWARHL FQKITGPINI FPENVINSTE IRRYYGS YN TLGKQLTIYE MWFYQDWVNK IEQSKAALQA TLIVRHDENK KLYVNFDLEI MQLIREAKCL DRQGIEIPES ARIILLQE D KFKTYYNELL YALKEYERIN SKIKPICKNL LLPHIEDLDL KLRPGMVTLT WTSMNIESYL YYVHQGLKKL EQLIINVND IIENRIENNL KTVSKVVLVH LPQDTKPLSL DSFVQLQEEY INSKTDFLTS KNVEVERAVD DLLQTIMLYP LDPHVDPVLP EETKRIKRY YFWYFYQALL NSTQNSLNAM KYRVCGKKIP GANTLQNLKP FFQVEVQLNG DKVTLNPSLQ EIQKSINRAA T AVLRCSKH LYNWDQQNKD STDKATFYDM IACDKEIVKV ILLLTGSIQG TKNKVNEFLS GFTKFEWLCK ESIQESIKKF SK NGPTLQN YEDQLKKFSQ IEEEIEKIVP TYKIGAMELM THNICTSLST WAKEWKLQYS QDLHKRARQL LDSLTEQTKM LST KLSKPV KDIDSLGYVM ETLEQIRKEQ AEIDMKFNPV QEMYSLLDNY LPGGITDKDE MDARSLLRRN WDILIQQAEI KGKE YQHKQ AIYLKELKQS IKDFTNQVSI FRRDYEKNGP MVEGISPAEA MERLRRFEDE YDVKYQMYKI NARGENLFGL QNQKY PELE KTDAEIKNLN KLYNLYDSVI KNIQQFKEKS WQDVSKDDLA KMEEDAGKYG EQCSRLPKDL KEWQAYRDLK NYIDSL REQ LPLIISLKKP SIMPRHWEKI KEITNTKLNY ENPDQFYIEE IMGAKLLDFR EDIEDITESA DKQLKIRTGL DEINLYW ND MQFQFGIWGK RDVPCMLNGL IVGTILERLE EDQLQLSTFN SQRHVTPFKA EVENLIRTFS DVNDTLDMWV KVQKLWTS L EPVFTGGDIA RQMPLQAKQF QGIDKNWMKI MEKAVETKKV IPCCQNDMLK DFLPDLNRKL EDCQKMLEAY LEGKRKKFP RFYFVSNPTL LKILSQGSEP TSIQEDFEKL FDAITKVTFE SAKDKKNPAL KQITQIQQVI GRNEENISLT GYYVKCEGNI EDWLKKLEQ NMQQTLKDIA SAAAQQVFQV GLKEFVSSQA SQIALLGLQI LWTSKVNEGL ERLSRNERNA MDIKRNEIKE H MNILSSMC LEDLNGAVER TKVETLVTIQ VHQKDISMDL KCKDVNDFEW QKQTRIAWKT DIDECIISIT DWDSPYSYEF LG AKERLCI TPLTDRCYIT LAQAMSMYYG GAPAGPAGTG KTETVKDLGR TLGVFVVVTN CSDQHRYRDM AKIFKGLVQS GLW GCFDEF NRIDLEVLSV VAMQVESITT ARKQHMKKFM FPEEEIEIEL IPTVSYFITM NPGYAGRQEL PENLKVLFRG VSMM VPDRE IIIKVKLASV GYLQIDLLAK KFNVLYRLCE EQLSKQRHYD FGLRNILSVL RTAGNTKRQE IKSDEEMLLM RSLRD MNLS KLVADDIPLF NGLLADIFPK LKEVPKKLYP DVEKKIPEEI NAESYLINTP SFQLKIIQLY ETCLVRHGFM LVGPTG SGK STIMKILTEV LTKLGSPHKI VIMNPKAITA EQMYGVKSEI SDDWIPGVFS TIWAKSNNRA LKHTTWITCD GPVDAIW IE NLNTVLDDNK ILTLANGERI AMTENCKVVF EVENLNNASP ATVSRCGQVY VSPTDLGYEA VIEGWIRNRK ASGRAEES D KLGNILRKYL INMRFIELQS KECKEPMMDT SPVISVINIL NLLTGCLQYF VQTQRTLSEQ EYEKFIVYSM AWAIGGIYE AQDRVRFHEL LLAKNAPIPQ KGKENETVFD YYVSQDYLDW KICSPEEWVP PQSLQFSQLL LPTLDSFRAE MLLNFILTQP KSHTCSNSA LLIGGSGTAK TSSVLLYCNK FDPQKMLFKR TNFSSATSPF MFQSTIEAEC DFKVGKEFAP PGNKMMTIFI D DMSMPFVN KWGDQITLEL VRQLIETGGF YMLDKTQRGN QRKMKNLQYI GAMNHPGGGR NDIPNRLKRQ FFIFNMILPL SI EGIYGPI IKHMFKQKYF SDSTYKVIES LTSATIALWN KVKSTMLPTP AKFHYVFNMR ELSRIFKGIL TCKKDTINDA PKS MKIKPE LFLVGLWRHE AERVLADKLV NNKDKDTVMG YIQEVSLESF SQIENEILEK YSSEKTFLFC DFLRPDVINE DGII EEEAP KIYEAIDSLT ELRKRCNFLL SFYNDRNPSK KMPLVLFDDA LKHLLRISRI IRQPRSSGLL VGVGGSGKQS LTRLA GFIG KNLIQQIIVT KTYSDKDLKE DIKKGFDDAG HLGKQVTFLM TDSEVKKEEF LEYINMVLST GEIPNLLAKD EREVWL GDI SQAYCKEKNL GNIDPPQSEL WTYFVDRVRD NFHIMLCFSP VGQKFRERAR KFPALFNECT IDWFLPWPEE ALVSVAE TF IKNFDKLDTK EETKQELMKH MGNVHLMVNE ICDEYYQKMR RQVYVTPKSF LSYLNSYKTL YIEKYDELDQ QEESFKIG L NKIQEATITI NQMEISLKEE EIQLNEATEK TNQLLANLDK ESKKANQKGE EVAATNKQCE IQAEQISKEK EEAERELEA ALPALRRAQE AVDSIESKDI VELKANKKPL DIIKYIMDAV LVFFKARLIP IQIEERVFNK KEGKAVLFLK ESYDESGIQT LGDMNFMKK LKEFEKDSIN EETIELLEPY LNQSEDWFND TFATKASKAA AGILKWAFAI YEYHQKSKIV KPKRIQVAIA E GRQAIALK ELEKAREDLA QIQAYIKNLK DVYTKQMEEK NELEMKAAKT KKKINTARTL ITSLSGEKDR WGKGAQDISD QK RKLVGNV SLSTAFISYC GPFNAEYRNK LAQQRFVVDM KKRGVPVTPG LELTSFLIDD ATIGEWNLQG LPKDDLSIQN GIM VTNSAR YPLFIDPQGQ GQNWIRNKLS ASIIPERCIT TLSHPKFKDM FLKYCMESGL TLIVENIENE VDPMMDPVLE RQII VKGKT QFVNVAGTEM ELSKEFKLFM TCRLANPSFS PELSAKTTII DFTVTQSGLE QQLLGKVISK EQKALEDSLN QLLAD VNQN QKDLQRLDKN LLERLINSQG NLLDDTELMD VLNNTKTQAK EVAAKLIDAE IKTKEINEKR EQYRPVAIRG SAIYFT MIE VSLVNWMYNS SLEQFLKLFI ESIDLSEKAQ LPSNRVKNII SFLTFHVYRY VNRGLFEKDK ITFILMMAFK ILTTAGT IS SGDVSLFLKS GDALDIKSER QKQISYLEDN QWLNILALSK HTFSGQTLPF FKELPDLISR SENQWRNWID KNDPENFP I PDFAESINQE KEIGSFISLC LVRSLRNDRT LIATQNFISN VLGKEFTDPI SYPIEGIWQE SSNMDPVLFL LSAGADPTS SIDELAKKKK KFPCEKVSMG EGQERVARQV IMKGFVEGGW VILQNCHLGL KFMEEIETLV SPINQIHEDF RLWITCEQHP KFPLGLLQK TLKVTNEPPK GLKAGLYKTF TTIITQEFID KVDHSNWRSL IFTICFLHSI VIERKKFGPL GWCVPYEYNY S DLEASLLY IEKYLTNLMS TPQPNSHNLP ISMNVVRYMI CEVQYGGRIT DDLDRELFIT YGETYLKDGI FGNDYFFYDI MV DGSGQKF KYRIPQNPSA ELIKYQEYIA KVPTVDNPEV FGLHSNADLT FRLKESKEMI NTVMETRPKD SSVGGGKTRE EIV QDKAKD MLKNLPPDYN DVEVRELVSK LGGPNPKTST ERGMTVPLNI FLYQEVTRMQ RVIGLVRKTL QDTILAIDGQ IIMT PEILE AINAIYDAKV PNSWLYDPSG AEISWLLPNL GSWSTSLSDR NKQLNDWLRS GQRPILFWLT GFFNPQGFLT GMKQE VTRN HKKGDGKGGE AWSLDDVVYS TTVKEREKEK DIEQPPAEGV YIKGLYLEGC KWSKNGLDDS DPKKIFADLP ILHVSA INK KKTNEQDRMS NTYLCPVYKY PKRTDKYLIF RVGLPCEGSN NPSHWKLRGV ALLCSTE

UniProtKB: Dynein heavy chain, outer arm protein

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Macromolecule #4: Dynein intermediate chain 2

MacromoleculeName: Dynein intermediate chain 2 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 77.888219 KDa
SequenceString: MPPKQTKVVA SRKTVMPISR AGRAQIRRKD SNTQNNMNDQ GMEDEEIDQQ REGMKNQYEQ LTAQELNEDM PSKMLEPKNP QAPKNITVY DYYTRKFKTD ELVDQMIVHF SMDGDYIWKE SNEYKTQEEI RDTKKALIKE AMRKQESEEP GANHDEEAIK Q TLRNKFNY ...String:
MPPKQTKVVA SRKTVMPISR AGRAQIRRKD SNTQNNMNDQ GMEDEEIDQQ REGMKNQYEQ LTAQELNEDM PSKMLEPKNP QAPKNITVY DYYTRKFKTD ELVDQMIVHF SMDGDYIWKE SNEYKTQEEI RDTKKALIKE AMRKQESEEP GANHDEEAIK Q TLRNKFNY NTRECQTINP SIRERGVSTE PPPSDTICGN ITQWEIFDAY YAEIMKDHQI ENKKKKEVDQ DKKQDQSMYS TS FKRCCKI MERMVVQNDQ EDKYHDYRYY WSQGDNLEAG KNEGHLLPIW RFSNEKQRKK NVTSICWNPL YPDLFAVSLG SYD FTKQRM GLICLYSLKN TTHPEYAFNC EAGVMCLDFH PKSAALLAVG LYDGTVLVYD IRNKHKKPIY QSTVRNQKHT DPVW QVKWN PDTSKNYNFY SISSDGRVMN WILMKNKLEP EEVILLRLVG KNEEESTLIG LACGLCFDFN KFEPHIFLVG TEEGK IHKC SRAYSGQYQE TYNGHLLAVY KVKWNNFHPR TFISASADWT VRIWDSKYTS QIICFDLSMM VVDAVWAPYS STVFAC ATM DKVQVYDLNV DKLNKLAEQK IVKQPKLTNL SFNYKDPILL VGDSHGGVTL VKLSPNLCKS GPEIKQTEDK KAMEEFK NV KIEDYEREKM ENLLAVVSKW EREDA

UniProtKB: Dynein intermediate chain 2

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Macromolecule #5: Flagellar outer dynein arm intermediate protein, putative

MacromoleculeName: Flagellar outer dynein arm intermediate protein, putative
type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 77.178062 KDa
SequenceString: MAEYFTYSKK RKEFNNPINF QDTETRYGGI QNQVVNINQY VQRNPNFIDL DNIAELSEHS VNTERVKTGD RGMSHKEGGW PGNVDPNEA QETGRFKKRI EKDTSFPQAV KDLKEGVEKC IYQNNQIDLL EEYFEGETSE HVVENLSSKT LMLFKDEKEI C KRSVSEIS ...String:
MAEYFTYSKK RKEFNNPINF QDTETRYGGI QNQVVNINQY VQRNPNFIDL DNIAELSEHS VNTERVKTGD RGMSHKEGGW PGNVDPNEA QETGRFKKRI EKDTSFPQAV KDLKEGVEKC IYQNNQIDLL EEYFEGETSE HVVENLSSKT LMLFKDEKEI C KRSVSEIS WHPEGPTKVA VSYAIMRFQQ MPEKMPTQAY VWDLLNPNSP EIKLMSPSAV TNISYNQKIP DQIGGGCYNG LL AVWDGRK GENPIMISPV ENSHYEPVTH FHWLMSKTGS ECVTTSTDGK VMWWDTRKFE AGPVEKLNII EGLGENEEII GGT ALEYNV EAGPSKFLIG TESGSILTAN KKLKKPVEIT TRYGLDQGRH LGPVYSINRS NQNPKYFLSV GDWSCKIWVE DLKT PIIRT KYHGSYLSDG CWSPTRSGAF FLVRRDGWMD VWDYYYRQNE IAFSHKVSDS PLTCIKINQT GGAYHNSGKL CAIGD QDGT VTILELCDSL YTMQPKEKDI INEMFEREYR KEKNLETIKK QQELAKRQVQ KDMGSQKEKW EKKKLEMIET AEASFH ENL AKNPVNEEEF NELDSPSEKR KKTNQNQGRE QEEQSREEQE ASGNFNQQQQ QQQEEEQQQE GEQQHHQNQE HQNGQGH EN GQEEGEENGE EGNQQENEGQ EENEQQQE

UniProtKB: Flagellar outer dynein arm intermediate protein, putative

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Macromolecule #6: Dynein light chain roadblock-type 2 protein

MacromoleculeName: Dynein light chain roadblock-type 2 protein / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 14.751817 KDa
SequenceString:
MAQDINADDQ LKQLSALEGA NGYVIFNESG IPLKRHEKSI SHEKAVHIAA LVSDLWNVSK KVIQRDLKTP ENDIEVIRLR TKHSYEYII TQSGDFTMLA IQLCGKAIEE AKKAAAAAAA AAVVQEENKE KEKK

UniProtKB: Dynein light chain roadblock-type 2 protein

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Macromolecule #7: Dynein light chain roadblock-type 2 protein

MacromoleculeName: Dynein light chain roadblock-type 2 protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 18.490188 KDa
SequenceString:
MIYKTSKEIQ KSDQRIIQQR VENSIINNQI YQKSLKRKTS KRKVRHQANK KNFQKEMSEV EDTLNRIKTH KTVLGYLIVN SEGGVVRGA FKDEEESKNI ANSIPLLTKK ARSVVRDLDP TNDLVFLRIQ TKLNEIMVAP DDEFSLIVIQ TKGEKRDEND

UniProtKB: Dynein light chain roadblock-type 2 protein

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Macromolecule #8: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 10.780357 KDa
SequenceString:
MSHLDKVQPV IKNSDMSVEM QKEVEEVAKK AIDYCNTDKE IATFIKDDFR SRYHGTWHCI VGRNFGSFVT FERSYYIYLY VGQLAILLF KTG

UniProtKB: Dynein light chain

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Macromolecule #9: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 12.348086 KDa
SequenceString:
MEQEKAVTDM DINELRKLMI GKAIINSSDM QGDLLQEAQD VIQSGIENNS APVLNIEAAC KYIKENLDKK FGPTWQCIIG EGYAYDVTV QNNTLLFMFY NGNLAVLIFK S

UniProtKB: Dynein light chain

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Macromolecule #10: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 10.973408 KDa
SequenceString:
MGDHANEQII DMPENSEMKS MKNDAFSQAK FAVENYKFEN KISSHIKKFF DEKYGPNWHC VVGKHFNAYV SYDSKNFIFF YEGQLAILL YRKG

UniProtKB: Dynein light chain

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Macromolecule #11: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 13.336089 KDa
SequenceString:
MASNQQQKED PKEQQQQYKT FMGARVLWPP ECADDILEGA IRETQDALKK FEIAREGQKI AEHLKKYMDD HFDPYWHVFF GKNFGCQAV HNKNRFIYFY IEKTAFLMYQ TQ

UniProtKB: Dynein light chain

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Macromolecule #12: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 12.516457 KDa
SequenceString:
MSDSDSDEGR VVEEPLPPHI IRFNDMAQHL LKKVIRQADV LIKENPQGLE KDIALNLVKF VKSQPEFKIG DGEWQCIIGK NFGCSLTFD ANVLAFFDLL PSRKSILLFK SG

UniProtKB: Dynein light chain

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Macromolecule #13: Dynein light chain

MacromoleculeName: Dynein light chain / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 10.453167 KDa
SequenceString:
MNHEPEVKAT DMEEDMIKRV KEIAINAVKE YKQEKQIAHY IKYEFDKIDG YGWNCIVGRN FGSHIIHQTK KYIFFKINEL CLLLWKA

UniProtKB: Dynein light chain

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Macromolecule #14: Dynein light chain 2A

MacromoleculeName: Dynein light chain 2A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 15.60812 KDa
SequenceString:
MKGTYLYLNI YKRKREASLI TLNYIKNRFY PSKIQKIIKE LFEDRLKGVE YDPNNANQLS ERLVLELREK IKRGKVPRYK IGVQVVFGE IKGQGLRIAS KCLWDVQNDN YASYTYTSEK VYCTGIVFGC YFE

UniProtKB: Dynein light chain 2A

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Macromolecule #15: Dynein light chain tctex-type 1 protein

MacromoleculeName: Dynein light chain tctex-type 1 protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 13.202817 KDa
SequenceString:
MGDTDKEYIS EEVQKAIDDS VKQVFGIKDD SSQVTITYNK DKVNLWTQQI IDYTIRGLNK LGKHFKYCVT AILQQTNHAG ISVQITAYQ DTNTDGSLIQ CYEINDIYAI VSVFAMAV

UniProtKB: Dynein light chain tctex-type 1 protein

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Macromolecule #16: Thioredoxin

MacromoleculeName: Thioredoxin / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 12.855699 KDa
SequenceString:
MSHPYIEITS TKQFEDILEK NEYVLVDFFA SWCGPCKILA EQFEPIKKDH KNLTIVKVNI DEEDLESIVE SHNVSSLPHV FLYHKKTVV KQFIGNQKDQ LWEMAKLSDA K

UniProtKB: Thioredoxin

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Macromolecule #17: Tubulin beta chain

MacromoleculeName: Tubulin beta chain / type: protein_or_peptide / ID: 17 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 49.617676 KDa
SequenceString: MREIVHIQGG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGRYV PRAILMDLEP GTMDSVRAGP FGQLFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAEGCDC LQGFQITHSL GGGTGSGMGT LLISKVREEY P DRIMETFS ...String:
MREIVHIQGG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLERINVY YNEATGGRYV PRAILMDLEP GTMDSVRAGP FGQLFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAEGCDC LQGFQITHSL GGGTGSGMGT LLISKVREEY P DRIMETFS VVPSPKVSDT VVEPYNATLS VHQLVENADE CMVIDNEALY DICFRTLKLT TPTYGDLNHL VSAAMSGVTC CL RFPGQLN SDLRKLAVNL IPFPRLHFFM IGFAPLTSRG SQQYRALTVP ELTQQMFDAK NMMCAADPRH GRYLTASALF RGR MSTKEV DEQMLNVQNK NSSYFVEWIP NNIKSSICDI PPKGLKMAVT FVGNSTAIQE MFKRVAEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATAEEEGE FEEEEGEN

UniProtKB: Tubulin beta chain

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Macromolecule #18: Tubulin alpha chain

MacromoleculeName: Tubulin alpha chain / type: protein_or_peptide / ID: 18 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 49.639035 KDa
SequenceString: MREVISIHVG QGGIQVGNAC WELFCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRAVFLDL EPTVIDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLCL DRIRKLADNC TGLQGFLVFN SVGGGTGSGL GSLLLERLSV D YGKKSKLG ...String:
MREVISIHVG QGGIQVGNAC WELFCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRAVFLDL EPTVIDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLCL DRIRKLADNC TGLQGFLVFN SVGGGTGSGL GSLLLERLSV D YGKKSKLG FTIYPSPQVS TAVVEPYNSI LSTHSLLEHT DVAVMLDNEA IYDICRRNLD IERPTYTNLN RLIAQVISSL TA SLRFDGA LNVDITEFQT NLVPYPRIHF MLSSYAPIIS AEKAYHEQLS VAEITNSAFE PANMMAKCDP RHGKYMACSM MYR GDVVPK DVNASIATIK TKRTIQFVDW CPTGFKVGIN YQPPTVVPGG DLAKVMRAVC MISNSTAIAE VFSRLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GIETAEGEGE EEGY

UniProtKB: Tubulin alpha chain

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Macromolecule #19: Outer dynein arm docking complex protein oda protein

MacromoleculeName: Outer dynein arm docking complex protein oda protein / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 35.237121 KDa
SequenceString: MDKSKYGNTA SNMNQSKQGN TLNAASTMKK PSVMGQTANA SKNLKKTEKS SEEEIMALEI QQVEIARLAK VFDQLRMASE TYGQGGNTS SKDNFNNSNN NISSAQIKDN NQSTTKDNNA NKKEGSEGKD YFTAKDVAII LHDLGYVATK AEIEQMIWEV D ENLDNKVN ...String:
MDKSKYGNTA SNMNQSKQGN TLNAASTMKK PSVMGQTANA SKNLKKTEKS SEEEIMALEI QQVEIARLAK VFDQLRMASE TYGQGGNTS SKDNFNNSNN NISSAQIKDN NQSTTKDNNA NKKEGSEGKD YFTAKDVAII LHDLGYVATK AEIEQMIWEV D ENLDNKVN SYEFNLMYRR CRYDKTGLEP RNLYNLVQFL MYLKSDTNVN SSSGANTAVA NNEQSHQKKT ITVEDTLELL YV RFGRQNL DYEIEEIFGK EEKNAQGEEK SITLKEFLDK QRKRDFAERK KLEAERKKVK MPEKEDDGYG GY

UniProtKB: EF hand protein

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Macromolecule #20: Docking complex 1/2 protein

MacromoleculeName: Docking complex 1/2 protein / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 11.081651 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

+
Macromolecule #21: Docking complex 1 protein

MacromoleculeName: Docking complex 1 protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 64.756031 KDa
SequenceString: MRASSATKSQ KDLKTLEEEY IHQSKKSNLL ENDRKIFHKN AEETKNNNMQ IIESLKKENK QLKTLRDELI ANKRASTPGM SKTQGSLVS WSGDIKDENY WRRKFDEARH ATKNKKSQLL QLQDKLNEVS DAKFGAVEES PLMRQIRILE NRLDKVMIKF N EAQSIRKT ...String:
MRASSATKSQ KDLKTLEEEY IHQSKKSNLL ENDRKIFHKN AEETKNNNMQ IIESLKKENK QLKTLRDELI ANKRASTPGM SKTQGSLVS WSGDIKDENY WRRKFDEARH ATKNKKSQLL QLQDKLNEVS DAKFGAVEES PLMRQIRILE NRLDKVMIKF N EAQSIRKT YEQIVKRLKE ERVGYDNQLA AIERSLKGKE HDFEELLLLA HDATHAKELA AAELKKYEHK KAAVRELRKT YI AEKRKAI EQREAVISRM EKKDKDNDDR NLEKSQANNL NELNNPQIEP QNHQDATFQR QKLNDYDEAF RKLYEATGVT DVN EIIQKF TTQDETSKSL KDLQREYQDT IDDKKKQRDD LKAGLNALKY EGNENPNRKQ LDEIEKNVNN AVNKCDKAKL KYER VSKIL VDVKAGIEHL YEKLEFYKLE GKPNIVITDE TLVEGLSQIV EKMKLIFQPV KNDPSYNPED FKQTAKGVSN YINLN LRDK SGRIESISKN IRVKLPEKDE EEVSNDEIED DIDIETTTKL KQKYQAQAKQ EKAARNKQKK QLGSTQQGRK V

UniProtKB: Uncharacterized protein

+
Macromolecule #22: Outer dynein arm docking complex protein oda protein

MacromoleculeName: Outer dynein arm docking complex protein oda protein / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Tetrahymena thermophila CU428 (eukaryote) / Strain: CU428
Molecular weightTheoretical: 63.455445 KDa
SequenceString: MNENLEKKKK MEELEEYQRK FRNLESDRKA YAEETVALIK KQRGIVDKLK NENQQLKDII SKMNAQKIQQ SNTMYGKPSS DSLVEELKQ KIEVERRQQM EIEKHVVDFQ KKIIEKRSNI GGYNAGAEND SSLAKQIKIL ENRLDKANQK FNEAIAVNKQ L RQQIDSLR ...String:
MNENLEKKKK MEELEEYQRK FRNLESDRKA YAEETVALIK KQRGIVDKLK NENQQLKDII SKMNAQKIQQ SNTMYGKPSS DSLVEELKQ KIEVERRQQM EIEKHVVDFQ KKIIEKRSNI GGYNAGAEND SSLAKQIKIL ENRLDKANQK FNEAIAVNKQ L RQQIDSLR RERVIFDNLY KKLEKELHEK RKQMANIIET ANTAYEERDR ANDQIQNLKM LAKKESENFE KDLRELSHIM EK NKKALDY IKLTEKNRDD NKLNNDLLDS DKFARTTSQK LYKDRNVNQT QSEKIQRYEE DFAKIQAATK VNDFEKLVNT FIE NEEKNF QTFKFVNELS NEIEELEKQI GELRSELDQY KGGSNMDIQY KRKIKEFEEV MTRAENKSES YEFKRHDAQK LINS LTNWI ETLFNTIECD KKVAKELAGS HSVTDGNMMI FLAIIENKVN QIVQAFSAID AQGANENYHT LLQNVSNLST ALMAN KQRQ DAPDNDEFEE EEGEGDRILN IEDFRKKALE KLDDRKQTQQ SKKLPKAITN RRKR

UniProtKB: Outer dynein arm docking complex protein oda protein

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Macromolecule #23: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 23 / Number of copies: 2 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

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Macromolecule #24: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 24 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #25: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 25 / Number of copies: 6 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

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Macromolecule #26: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 26 / Number of copies: 6 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Macromolecule #27: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 27 / Number of copies: 6 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

Concentration3 mg/mL
BufferpH: 7.4
GridModel: C-flat-2/1 / Support film - Material: CARBON / Support film - topology: HOLEY
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK IV / Details: Blot force 3 for 5 seconds.

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-40 / Average exposure time: 4.0 sec. / Average electron dose: 45.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.2 µm / Nominal magnification: 59000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Startup modelType of model: EMDB MAP
EMDB ID:

8537

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 8.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 139548
Initial angle assignmentType: PROJECTION MATCHING / Software - Name: SPIDER (ver. 19.02) / Details: Hel
Final angle assignmentType: PROJECTION MATCHING / Software: (Name: RELION (ver. 3.0), FREALIGN (ver. 9.03))
Final 3D classificationNumber classes: 3 / Software - Name: FREALIGN (ver. 9.03)

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Atomic model buiding 1

RefinementSpace: REAL / Protocol: AB INITIO MODEL / Overall B value: 179
Output model

PDB-7moq:
The structure of the Tetrahymena thermophila outer dynein arm on doublet microtubule

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