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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-2209 | |||||||||
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Title | Organization of the Influenza Virus Replication Machinery | |||||||||
![]() | Influenza RNP Central filament region | |||||||||
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![]() | Influenza / RNP / Polymerase | |||||||||
Function / homology | ![]() helical viral capsid / viral penetration into host nucleus / host cell / viral nucleocapsid / ribonucleoprotein complex / symbiont entry into host cell / host cell nucleus / structural molecule activity / RNA binding / identical protein binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 21.0 Å | |||||||||
![]() | Moeller A / Kirchdoerfer RN / Potter CS / Carragher B / Wilson IA | |||||||||
![]() | ![]() Title: Organization of the influenza virus replication machinery. Authors: Arne Moeller / Robert N Kirchdoerfer / Clinton S Potter / Bridget Carragher / Ian A Wilson / ![]() Abstract: Influenza virus ribonucleoprotein complexes (RNPs) are central to the viral life cycle and in adaptation to new host species. RNPs are composed of the viral genome, viral polymerase, and many copies ...Influenza virus ribonucleoprotein complexes (RNPs) are central to the viral life cycle and in adaptation to new host species. RNPs are composed of the viral genome, viral polymerase, and many copies of the viral nucleoprotein. In vitro cell expression of all RNP protein components with four of the eight influenza virus gene segments enabled structural determination of native influenza virus RNPs by means of cryogenic electron microscopy (cryo-EM). The cryo-EM structure reveals the architecture and organization of the native RNP, defining the attributes of its largely helical structure and how polymerase interacts with nucleoprotein and the viral genome. Observations of branched-RNP structures in negative-stain electron microscopy and their putative identification as replication intermediates suggest a mechanism for viral replication by a second polymerase on the RNP template. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 10.3 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 9.2 KB 9.2 KB | Display Display | ![]() |
Images | ![]() | 431.2 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ymnMC ![]() 2211C ![]() 2212C ![]() 2213C C: citing same article ( M: atomic model generated by this map |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | Influenza RNP Central filament region | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.64 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : influenza virus ribonucleoprotein complex central filament region
Entire | Name: influenza virus ribonucleoprotein complex central filament region |
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Components |
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-Supramolecule #1000: influenza virus ribonucleoprotein complex central filament region
Supramolecule | Name: influenza virus ribonucleoprotein complex central filament region type: sample / ID: 1000 / Number unique components: 2 |
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-Macromolecule #1: influenza virus ribonucleoprotein complex
Macromolecule | Name: influenza virus ribonucleoprotein complex / type: protein_or_peptide / ID: 1 / Name.synonym: Influenza RNP Details: Helical assembly of one strand of single stranded RNA bound to several nucleoproteins Recombinant expression: Yes |
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Source (natural) | Organism: ![]() |
Recombinant expression | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | helical reconstruction |
Aggregation state | filament |
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Sample preparation
Vitrification | Cryogen name: ETHANE / Chamber humidity: 85 % / Instrument: FEI VITROBOT MARK II |
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Electron microscopy
Microscope | FEI TECNAI 20 |
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Date | Aug 27, 2010 |
Image recording | Category: CCD / Film or detector model: GENERIC CCD / Number real images: 3437 / Average electron dose: 15 e/Å2 |
Electron beam | Acceleration voltage: 120 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal magnification: 50000 |
Sample stage | Specimen holder model: GATAN LIQUID NITROGEN |
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Image processing
Final reconstruction | Algorithm: OTHER / Resolution.type: BY AUTHOR / Resolution: 21.0 Å / Resolution method: FSC 0.5 CUT-OFF Software - Name: IHRSR, XMIPP, IMAGIC, SPIDER, APPION, CTFFIND3 |
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CTF correction | Details: wholeimage |