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- PDB-1xk5: Crystal structure of the m3G-cap-binding domain of snurportin1 in... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1xk5 | ||||||
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Title | Crystal structure of the m3G-cap-binding domain of snurportin1 in complex with a m3GpppG-cap dinucleotide | ||||||
![]() | snurportin-1 | ||||||
![]() | TRANSPORT PROTEIN / Protein-RNA-complex | ||||||
Function / homology | ![]() RNA import into nucleus / RNA cap binding / snRNA import into nucleus / NLS-dependent protein nuclear import complex / nuclear import signal receptor activity / nuclear pore / protein import into nucleus / snRNP Assembly / nucleoplasm / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Strasser, A. / Dickmanns, A. / Luehrmann, R. / Ficner, R. | ||||||
![]() | ![]() Title: Structural basis for m(3)G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1 Authors: Strasser, A. / Dickmanns, A. / Luehrmann, R. / Ficner, R. #1: Journal: ACTA CRYSTALLOGR.,SECT.D / Year: 2004 Title: Purification, crystallization and preliminary crystallographic data of the m3G cap-binding domain of human snRNP import factor snurportin 1 Authors: Strasser, A. / Dickmanns, A. / Schmidt, U. / Penka, E. / Urlaub, H. / Sekine, M. / Luehrmann, R. / Ficner, R. #2: Journal: Embo J. / Year: 1998 Title: Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure Authors: Huber, J. / Cronshagen, U. / Kadokura, M. / Marshallsay, C. / Wada, T. / Sekine, M. / Luehrmann, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 57.2 KB | Display | ![]() |
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PDB format | ![]() | 41.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 882.9 KB | Display | ![]() |
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Full document | ![]() | 895.2 KB | Display | |
Data in XML | ![]() | 13.2 KB | Display | |
Data in CIF | ![]() | 17.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23149.438 Da / Num. of mol.: 1 Fragment: m3G-cap-binding domain comprising amino acids 97-300 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-TPG / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.4 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: 8% PEG20K, 100mM MES pH6.0 for initial crystals and 200mM sodium citrate pH5.5 for larger crystals after seeding, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Dec 18, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→30 Å / Num. obs: 249994 / Redundancy: 11 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 18.7 |
Reflection shell | Resolution: 2.4→2.49 Å |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.4→20 Å
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Refine LS restraints |
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LS refinement shell |
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