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- PDB-1ws2: urate oxidase from aspergillus flavus complexed with 5,6-diaminouracil -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ws2 | ||||||
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Title | urate oxidase from aspergillus flavus complexed with 5,6-diaminouracil | ||||||
![]() | Uricase | ||||||
![]() | OXIDOREDUCTASE / uric acid degradation / dimeric barrel / tunnel-shaped protein | ||||||
Function / homology | ![]() purine nucleobase catabolic process / factor-independent urate hydroxylase / urate oxidase activity / urate catabolic process / peroxisome Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Retailleau, P. / Colloc'h, N. / Vivares, D. / Bonnete, F. / Castro, B. / El Hajji, M. / Prange, T. | ||||||
![]() | ![]() Title: Urate oxidase from Aspergillus flavus: new crystal-packing contacts in relation to the content of the active site. Authors: Retailleau, P. / Colloc'h, N. / Vivares, D. / Bonnete, F. / Castro, B. / El Hajji, M. / Prange, T. #1: Journal: Nat.Struct.Biol. / Year: 1997 Title: Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 A resolution Authors: Colloc'h, N. / El Hajji, M. / Bachet, B. / L'Hermite, G. / Schiltz, M. / Castro, B. / Mornon, J.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 241.1 KB | Display | ![]() |
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PDB format | ![]() | 196.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 489.6 KB | Display | ![]() |
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Full document | ![]() | 528.7 KB | Display | |
Data in XML | ![]() | 47.3 KB | Display | |
Data in CIF | ![]() | 62.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1wrrC ![]() 1ws3C ![]() 1xt4C ![]() 1xxjC ![]() 1xy3C ![]() 1r51S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The asymmetric unit contains the biological homotetrameric assembly |
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Components
#1: Protein | Mass: 34199.586 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q00511, factor-independent urate hydroxylase #2: Chemical | ChemComp-URN / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 61.25 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8 Details: pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 291 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 13, 2003 / Details: CURVATED MIRRORS |
Radiation | Monochromator: SI (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.972 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→32.1 Å / Num. obs: 48554 / % possible obs: 99.9 % / Redundancy: 12.5 % / Rsym value: 0.087 / Net I/σ(I): 15.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1R51 Resolution: 2.7→15 Å / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 41.923 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.77 Å
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