+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1uio | ||||||
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タイトル | ADENOSINE DEAMINASE (HIS 238 ALA MUTANT) | ||||||
要素 | ADENOSINE DEAMINASE | ||||||
キーワード | HYDROLASE / NUCLEOTIDE METABOLISM / ZINC COFACTOR TIM BARREL | ||||||
機能・相同性 | 機能・相同性情報 mature B cell apoptotic process / xanthine biosynthetic process / negative regulation of penile erection / Purine salvage / Ribavirin ADME / negative regulation of circadian sleep/wake cycle, non-REM sleep / negative regulation of mucus secretion / penile erection / purine nucleoside binding / positive regulation of germinal center formation ...mature B cell apoptotic process / xanthine biosynthetic process / negative regulation of penile erection / Purine salvage / Ribavirin ADME / negative regulation of circadian sleep/wake cycle, non-REM sleep / negative regulation of mucus secretion / penile erection / purine nucleoside binding / positive regulation of germinal center formation / negative regulation of adenosine receptor signaling pathway / histamine secretion / cytoplasmic vesicle lumen / inosine biosynthetic process / 2'-deoxyadenosine deaminase activity / amide catabolic process / adenosine deaminase / hypoxanthine biosynthetic process / germinal center B cell differentiation / adenosine catabolic process / adenosine deaminase activity / inhibition of non-skeletal tissue mineralization / hypoxanthine salvage / deoxyadenosine catabolic process / dAMP catabolic process / AMP catabolic process / adenosine metabolic process / positive regulation of T cell differentiation in thymus / dATP catabolic process / mucus secretion / negative regulation of leukocyte migration / regulation of cell-cell adhesion mediated by integrin / allantoin metabolic process / embryonic digestive tract development / trophectodermal cell differentiation / response to purine-containing compound / GMP salvage / IMP salvage / positive regulation of smooth muscle contraction / Peyer's patch development / germinal center formation / negative regulation of mature B cell apoptotic process / AMP salvage / regulation of T cell differentiation in thymus / negative regulation of thymocyte apoptotic process / anchoring junction / positive regulation of alpha-beta T cell differentiation / alpha-beta T cell differentiation / positive regulation of T cell differentiation / regulation of T cell differentiation / positive regulation of heart rate / leukocyte migration / lung alveolus development / positive regulation of T cell receptor signaling pathway / thymocyte apoptotic process / B cell proliferation / T cell differentiation / smooth muscle contraction / : / response to vitamin E / positive regulation of B cell proliferation / T cell activation / positive regulation of calcium-mediated signaling / dendrite cytoplasm / lung development / liver development / placenta development / calcium-mediated signaling / negative regulation of inflammatory response / positive regulation of T cell activation / T cell receptor signaling pathway / T cell differentiation in thymus / in utero embryonic development / lysosome / response to hypoxia / cell adhesion / external side of plasma membrane / neuronal cell body / negative regulation of apoptotic process / apoptotic process / extracellular space / zinc ion binding / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | Mus musculus (ハツカネズミ) | ||||||
手法 | X線回折 / 解像度: 2.4 Å | ||||||
データ登録者 | Wilson, D.K. / Quiocho, F.A. | ||||||
引用 | ジャーナル: Biochemistry / 年: 1996 タイトル: Site-directed mutagenesis of histidine 238 in mouse adenosine deaminase: substitution of histidine 238 does not impede hydroxylate formation. 著者: Sideraki, V. / Wilson, D.K. / Kurz, L.C. / Quiocho, F.A. / Rudolph, F.B. #1: ジャーナル: Biochemistry / 年: 1996 タイトル: Probing the Functional Role of Two Conserved Active Site Aspartates in Mouse Adenosine Deaminase 著者: Sideraki, V. / Mohamedali, K.A. / Wilson, D.K. / Chang, Z. / Kellems, R.E. / Quiocho, F.A. / Rudolph, F.B. #2: ジャーナル: Biochemistry / 年: 1993 タイトル: A Pre-Transition-State Mimic of an Enzyme: X-Ray Structure of Adenosine Deaminase with Bound 1-Deazaadenosine and Zinc-Activated Water 著者: Wilson, D.K. / Quiocho, F.A. #3: ジャーナル: Science / 年: 1991 タイトル: Atomic Structure of Adenosine Deaminase Complexed with a Transition-State Analog: Understanding Catalysis and Immunodeficiency Mutations 著者: Wilson, D.K. / Rudolph, F.B. / Quiocho, F.A. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1uio.cif.gz | 86.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1uio.ent.gz | 63.8 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1uio.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/ui/1uio ftp://data.pdbj.org/pub/pdb/validation_reports/ui/1uio | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 39648.117 Da / 分子数: 1 / 変異: H238A / 由来タイプ: 組換発現 / 由来: (組換発現) Mus musculus (ハツカネズミ) / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P03958, adenosine deaminase |
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#2: 化合物 | ChemComp-ZN / |
#3: 化合物 | ChemComp-HPR / |
#4: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: X線回折 |
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-試料調製
結晶 | マシュー密度: 3.38 Å3/Da / 溶媒含有率: 63.59 % | |||||||||||||||
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結晶化 | *PLUS pH: 4.2 / 手法: unknown / 詳細: Wilson, D.K., (1991) Science, 252, 1278. | |||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射光源 | 由来: SEALED TUBE / 波長: 1.5418 |
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検出器 | タイプ: XUONG-HAMLIN MULTIWIRE / 検出器: AREA DETECTOR / 日付: 1995年1月16日 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | Num. obs: 22029 / Observed criterion σ(I): 1 / 冗長度: 3.2 % / Rmerge(I) obs: 0.052 |
反射 | *PLUS Num. measured all: 71281 |
-解析
ソフトウェア |
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精密化 | 解像度: 2.4→10 Å / σ(F): 1 /
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精密化ステップ | サイクル: LAST / 解像度: 2.4→10 Å
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拘束条件 |
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