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Open data
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Basic information
Entry | Database: PDB / ID: 1fkw | ||||||
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Title | MURINE ADENOSINE DEAMINASE (D295E) | ||||||
![]() | ADENOSINE DEAMINASE | ||||||
![]() | AMINOHYDROLASE / ZINC COFACTOR / TIM BARREL | ||||||
Function / homology | ![]() mature B cell apoptotic process / xanthine biosynthetic process / negative regulation of penile erection / Purine salvage / Ribavirin ADME / negative regulation of circadian sleep/wake cycle, non-REM sleep / negative regulation of mucus secretion / penile erection / purine nucleoside binding / positive regulation of germinal center formation ...mature B cell apoptotic process / xanthine biosynthetic process / negative regulation of penile erection / Purine salvage / Ribavirin ADME / negative regulation of circadian sleep/wake cycle, non-REM sleep / negative regulation of mucus secretion / penile erection / purine nucleoside binding / positive regulation of germinal center formation / negative regulation of adenosine receptor signaling pathway / histamine secretion / cytoplasmic vesicle lumen / 2'-deoxyadenosine deaminase activity / inosine biosynthetic process / amide catabolic process / adenosine deaminase / hypoxanthine biosynthetic process / germinal center B cell differentiation / adenosine catabolic process / adenosine deaminase activity / inhibition of non-skeletal tissue mineralization / hypoxanthine salvage / deoxyadenosine catabolic process / dAMP catabolic process / adenosine metabolic process / positive regulation of T cell differentiation in thymus / AMP catabolic process / dATP catabolic process / mucus secretion / negative regulation of leukocyte migration / regulation of cell-cell adhesion mediated by integrin / response to purine-containing compound / embryonic digestive tract development / allantoin metabolic process / trophectodermal cell differentiation / GMP salvage / IMP salvage / positive regulation of smooth muscle contraction / Peyer's patch development / germinal center formation / negative regulation of mature B cell apoptotic process / AMP salvage / regulation of T cell differentiation in thymus / negative regulation of thymocyte apoptotic process / anchoring junction / positive regulation of alpha-beta T cell differentiation / positive regulation of T cell differentiation / alpha-beta T cell differentiation / regulation of T cell differentiation / positive regulation of heart rate / leukocyte migration / lung alveolus development / positive regulation of T cell receptor signaling pathway / thymocyte apoptotic process / B cell proliferation / T cell differentiation / smooth muscle contraction / : / response to vitamin E / positive regulation of calcium-mediated signaling / positive regulation of B cell proliferation / T cell activation / dendrite cytoplasm / liver development / calcium-mediated signaling / lung development / placenta development / negative regulation of inflammatory response / positive regulation of T cell activation / T cell receptor signaling pathway / T cell differentiation in thymus / in utero embryonic development / lysosome / cell adhesion / response to hypoxia / external side of plasma membrane / neuronal cell body / apoptotic process / negative regulation of apoptotic process / extracellular space / zinc ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Wilson, D.K. / Quiocho, F.A. | ||||||
![]() | ![]() Title: Probing the functional role of two conserved active site aspartates in mouse adenosine deaminase. Authors: Sideraki, V. / Mohamedali, K.A. / Wilson, D.K. / Chang, Z. / Kellems, R.E. / Quiocho, F.A. / Rudolph, F.B. #1: ![]() Title: A Pre-Transition-State Mimic of an Enzyme: X-Ray Structure of Adenosine Deaminase with Bound 1-Deazaadenosine and Zinc-Activated Water Authors: Wilson, D.K. / Quiocho, F.A. #2: ![]() Title: Atomic Structure of Adenosine Deaminase Complexed with a Transition-State Analog: Understanding Catalysis and Immunodeficiency Mutations Authors: Wilson, D.K. / Rudolph, F.B. / Quiocho, F.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 84.2 KB | Display | ![]() |
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PDB format | ![]() | 62.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.9 KB | Display | ![]() |
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Full document | ![]() | 457.5 KB | Display | |
Data in XML | ![]() | 9.3 KB | Display | |
Data in CIF | ![]() | 13.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 39729.215 Da / Num. of mol.: 1 / Mutation: D295E Source method: isolated from a genetically manipulated source Details: LIGANDED TO ZINC, PURINE RIBOSIDE / Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-PUR / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.55 Å3/Da / Density % sol: 65.31 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 4.2 / Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Sep 6, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 24014 / Redundancy: 1.7 % / Rmerge(I) obs: 0.0512 |
Reflection | *PLUS Highest resolution: 2.4 Å / Num. measured all: 40777 / Rmerge(I) obs: 0.051 |
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Processing
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Refinement | Resolution: 2.4→8 Å / σ(F): 1 Details: THE "EFFECTOR" REGION (RESIDUES 40 - 67) IS INVISIBLE AND DOMAIN III IS DISORDERED AND POORLY VISIBLE IN MAPS. ASSIGNMENT OF SEQUENCE OF THE MODEL OF DOMAIN III IS VERY UNCERTAIN (RESIDUES ...Details: THE "EFFECTOR" REGION (RESIDUES 40 - 67) IS INVISIBLE AND DOMAIN III IS DISORDERED AND POORLY VISIBLE IN MAPS. ASSIGNMENT OF SEQUENCE OF THE MODEL OF DOMAIN III IS VERY UNCERTAIN (RESIDUES 400 - 475). TEMPERATURE FACTORS HAVE NOT BEEN REFINED AND SOLVENT MOLECULES HAVE NOT BEEN INCLUDED.
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Refinement step | Cycle: LAST / Resolution: 2.4→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_deg / Dev ideal: 1.71 |