+Open data
-Basic information
Entry | Database: PDB / ID: 1uio | ||||||
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Title | ADENOSINE DEAMINASE (HIS 238 ALA MUTANT) | ||||||
Components | ADENOSINE DEAMINASE | ||||||
Keywords | HYDROLASE / NUCLEOTIDE METABOLISM / ZINC COFACTOR TIM BARREL | ||||||
Function / homology | Function and homology information mature B cell apoptotic process / xanthine biosynthetic process / negative regulation of penile erection / Purine salvage / Ribavirin ADME / negative regulation of circadian sleep/wake cycle, non-REM sleep / negative regulation of mucus secretion / penile erection / purine nucleoside binding / positive regulation of germinal center formation ...mature B cell apoptotic process / xanthine biosynthetic process / negative regulation of penile erection / Purine salvage / Ribavirin ADME / negative regulation of circadian sleep/wake cycle, non-REM sleep / negative regulation of mucus secretion / penile erection / purine nucleoside binding / positive regulation of germinal center formation / negative regulation of adenosine receptor signaling pathway / histamine secretion / cytoplasmic vesicle lumen / inosine biosynthetic process / 2'-deoxyadenosine deaminase activity / amide catabolic process / adenosine deaminase / hypoxanthine biosynthetic process / germinal center B cell differentiation / adenosine catabolic process / adenosine deaminase activity / inhibition of non-skeletal tissue mineralization / hypoxanthine salvage / deoxyadenosine catabolic process / dAMP catabolic process / AMP catabolic process / adenosine metabolic process / positive regulation of T cell differentiation in thymus / dATP catabolic process / mucus secretion / negative regulation of leukocyte migration / regulation of cell-cell adhesion mediated by integrin / allantoin metabolic process / embryonic digestive tract development / trophectodermal cell differentiation / response to purine-containing compound / GMP salvage / IMP salvage / positive regulation of smooth muscle contraction / Peyer's patch development / germinal center formation / negative regulation of mature B cell apoptotic process / AMP salvage / regulation of T cell differentiation in thymus / negative regulation of thymocyte apoptotic process / anchoring junction / positive regulation of alpha-beta T cell differentiation / alpha-beta T cell differentiation / positive regulation of T cell differentiation / regulation of T cell differentiation / positive regulation of heart rate / leukocyte migration / lung alveolus development / positive regulation of T cell receptor signaling pathway / thymocyte apoptotic process / B cell proliferation / T cell differentiation / smooth muscle contraction / : / response to vitamin E / positive regulation of B cell proliferation / T cell activation / positive regulation of calcium-mediated signaling / dendrite cytoplasm / lung development / liver development / placenta development / calcium-mediated signaling / negative regulation of inflammatory response / positive regulation of T cell activation / T cell receptor signaling pathway / T cell differentiation in thymus / in utero embryonic development / lysosome / response to hypoxia / cell adhesion / external side of plasma membrane / neuronal cell body / negative regulation of apoptotic process / apoptotic process / extracellular space / zinc ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.4 Å | ||||||
Authors | Wilson, D.K. / Quiocho, F.A. | ||||||
Citation | Journal: Biochemistry / Year: 1996 Title: Site-directed mutagenesis of histidine 238 in mouse adenosine deaminase: substitution of histidine 238 does not impede hydroxylate formation. Authors: Sideraki, V. / Wilson, D.K. / Kurz, L.C. / Quiocho, F.A. / Rudolph, F.B. #1: Journal: Biochemistry / Year: 1996 Title: Probing the Functional Role of Two Conserved Active Site Aspartates in Mouse Adenosine Deaminase Authors: Sideraki, V. / Mohamedali, K.A. / Wilson, D.K. / Chang, Z. / Kellems, R.E. / Quiocho, F.A. / Rudolph, F.B. #2: Journal: Biochemistry / Year: 1993 Title: A Pre-Transition-State Mimic of an Enzyme: X-Ray Structure of Adenosine Deaminase with Bound 1-Deazaadenosine and Zinc-Activated Water Authors: Wilson, D.K. / Quiocho, F.A. #3: Journal: Science / Year: 1991 Title: Atomic Structure of Adenosine Deaminase Complexed with a Transition-State Analog: Understanding Catalysis and Immunodeficiency Mutations Authors: Wilson, D.K. / Rudolph, F.B. / Quiocho, F.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1uio.cif.gz | 86.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1uio.ent.gz | 63.8 KB | Display | PDB format |
PDBx/mmJSON format | 1uio.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ui/1uio ftp://data.pdbj.org/pub/pdb/validation_reports/ui/1uio | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 39648.117 Da / Num. of mol.: 1 / Mutation: H238A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Production host: Escherichia coli (E. coli) / References: UniProt: P03958, adenosine deaminase |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-HPR / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.38 Å3/Da / Density % sol: 63.59 % | |||||||||||||||
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Crystal grow | *PLUS pH: 4.2 / Method: unknown / Details: Wilson, D.K., (1991) Science, 252, 1278. | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SEALED TUBE / Wavelength: 1.5418 |
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Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Jan 16, 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 22029 / Observed criterion σ(I): 1 / Redundancy: 3.2 % / Rmerge(I) obs: 0.052 |
Reflection | *PLUS Num. measured all: 71281 |
-Processing
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Refinement | Resolution: 2.4→10 Å / σ(F): 1 /
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Refinement step | Cycle: LAST / Resolution: 2.4→10 Å
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Refine LS restraints |
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