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- PDB-1iay: CRYSTAL STRUCTURE OF ACC SYNTHASE COMPLEXED WITH COFACTOR PLP AND... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1iay | ||||||
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Title | CRYSTAL STRUCTURE OF ACC SYNTHASE COMPLEXED WITH COFACTOR PLP AND INHIBITOR AVG | ||||||
![]() | 1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 2 | ||||||
![]() | LYASE / protein-cofactor-inhibitor complex / V6-dependent enzyme | ||||||
Function / homology | ![]() 1-aminocyclopropane-1-carboxylate synthase / fruit ripening / 1-aminocyclopropane-1-carboxylate synthase activity / ethylene biosynthetic process / amino acid metabolic process / transaminase activity / pyridoxal phosphate binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huai, Q. / Xia, Y. / Chen, Y. / Callahan, B. / Li, N. / Ke, H. | ||||||
![]() | ![]() Title: Crystal structures of 1-aminocyclopropane-1-carboxylate (ACC) synthase in complex with aminoethoxyvinylglycine and pyridoxal-5'-phosphate provide new insight into catalytic mechanisms Authors: Huai, Q. / Xia, Y. / Chen, Y. / Callahan, B. / Li, N. / Ke, H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 91.3 KB | Display | ![]() |
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PDB format | ![]() | 73.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 48294.113 Da / Num. of mol.: 1 / Fragment: RESIDUES 11-438 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P18485, 1-aminocyclopropane-1-carboxylate synthase |
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#2: Chemical | ChemComp-PLP / |
#3: Chemical | ChemComp-AVG / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.42 Å3/Da / Density % sol: 49.12 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 0.1 M Na citrate, 18% PEG3350, 50 mM MgCl2, 10% glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 296K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: BRANDEIS - B4 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. all: 13199 / Num. obs: 13199 / % possible obs: 97.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.7 % / Rmerge(I) obs: 0.109 / Net I/σ(I): 6 |
Reflection shell | Resolution: 2.7→2.9 Å / Rmerge(I) obs: 0.445 / % possible all: 76.2 |
Reflection | *PLUS Num. measured all: 62671 |
Reflection shell | *PLUS % possible obs: 76.2 % / Mean I/σ(I) obs: 1.5 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 30.1 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→50 Å
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Refine LS restraints |
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||
Refine LS restraints | *PLUS
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