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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1f42 | |||||||||
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タイトル | THE P40 DOMAIN OF HUMAN INTERLEUKIN-12 | |||||||||
![]() | INTERLEUKIN-12 BETA CHAIN | |||||||||
![]() | CYTOKINE | |||||||||
機能・相同性 | ![]() late endosome lumen / regulation of tyrosine phosphorylation of STAT protein / interleukin-12 alpha subunit binding / interleukin-12 complex / interleukin-23 complex / natural killer cell activation involved in immune response / positive regulation of natural killer cell activation / positive regulation of lymphocyte proliferation / positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target / negative regulation of vascular endothelial growth factor signaling pathway ...late endosome lumen / regulation of tyrosine phosphorylation of STAT protein / interleukin-12 alpha subunit binding / interleukin-12 complex / interleukin-23 complex / natural killer cell activation involved in immune response / positive regulation of natural killer cell activation / positive regulation of lymphocyte proliferation / positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target / negative regulation of vascular endothelial growth factor signaling pathway / negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis / positive regulation of tissue remodeling / positive regulation of activation of Janus kinase activity / sexual reproduction / positive regulation of T-helper 1 type immune response / positive regulation of NK T cell activation / positive regulation of smooth muscle cell apoptotic process / positive regulation of mononuclear cell proliferation / interleukin-12 receptor binding / positive regulation of memory T cell differentiation / natural killer cell activation / T-helper cell differentiation / positive regulation of osteoclast differentiation / Interleukin-23 signaling / negative regulation of interleukin-17 production / positive regulation of T-helper 17 type immune response / positive regulation of NK T cell proliferation / interleukin-12-mediated signaling pathway / Interleukin-12 signaling / response to UV-B / positive regulation of granulocyte macrophage colony-stimulating factor production / positive regulation of natural killer cell proliferation / cytokine receptor activity / T-helper 1 type immune response / negative regulation of interleukin-10 production / cytokine binding / positive regulation of activated T cell proliferation / defense response to protozoan / positive regulation of interleukin-17 production / Interleukin-10 signaling / positive regulation of interleukin-10 production / positive regulation of cell adhesion / negative regulation of protein secretion / positive regulation of T-helper 17 cell lineage commitment / T cell proliferation / positive regulation of T cell proliferation / positive regulation of tyrosine phosphorylation of STAT protein / negative regulation of inflammatory response to antigenic stimulus / positive regulation of defense response to virus by host / regulation of cytokine production / positive regulation of interleukin-12 production / cytokine activity / negative regulation of smooth muscle cell proliferation / cytokine-mediated signaling pathway / cellular response to type II interferon / positive regulation of inflammatory response / positive regulation of non-canonical NF-kappaB signal transduction / positive regulation of T cell mediated cytotoxicity / positive regulation of tumor necrosis factor production / positive regulation of type II interferon production / cell migration / cellular response to lipopolysaccharide / Interleukin-4 and Interleukin-13 signaling / defense response to virus / defense response to Gram-negative bacterium / receptor complex / protein heterodimerization activity / endoplasmic reticulum lumen / external side of plasma membrane / protein-containing complex binding / extracellular space / extracellular region / identical protein binding / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | ![]() | |||||||||
![]() | Yoon, C. / Johnston, S.C. / Tang, J. / Tobin, J.F. / Somers, W.S. | |||||||||
![]() | ![]() タイトル: Charged residues dominate a unique interlocking topography in the heterodimeric cytokine interleukin-12. 著者: Yoon, C. / Johnston, S.C. / Tang, J. / Stahl, M. / Tobin, J.F. / Somers, W.S. | |||||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 70.4 KB | 表示 | ![]() |
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PDB形式 | ![]() | 55.5 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 470.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 472.5 KB | 表示 | |
XML形式データ | ![]() | 8.3 KB | 表示 | |
CIF形式データ | ![]() | 12.3 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 34739.945 Da / 分子数: 1 / 断片: RESIDUES 23-328 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 参照: UniProt: P29460 | ||
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#2: 多糖 | alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | ||
#3: 化合物 | #4: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.55 Å3/Da / 溶媒含有率: 51.67 % | ||||||||||||||||||||||||||||||
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結晶化 | 温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6.5 詳細: 18% PEG8000, 0.2M Calcium Acetate, 0.1M Na Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||
結晶化 | *PLUS 温度: 18 ℃ / pH: 8 | ||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: RIGAKU RAXIS IIC / 検出器: IMAGE PLATE / 日付: 1997年6月1日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.542 Å / 相対比: 1 |
反射 | 解像度: 2.5→15 Å / Num. all: 12537 / Num. obs: 90266 / % possible obs: 97 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / 冗長度: 7.2 % / Biso Wilson estimate: 40 Å2 / Rmerge(I) obs: 0.054 / Net I/σ(I): 35 |
反射 シェル | 解像度: 2.5→2.59 Å / 冗長度: 3 % / Rmerge(I) obs: 0.16 / % possible all: 79 |
反射 | *PLUS Num. obs: 12537 / Num. measured all: 90266 |
反射 シェル | *PLUS 最高解像度: 2.5 Å / % possible obs: 79 % / Rmerge(I) obs: 0.16 |
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解析
ソフトウェア |
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精密化 | 解像度: 2.5→15 Å / σ(F): 2 / σ(I): 0 / 立体化学のターゲット値: Engh & Huber
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精密化ステップ | サイクル: LAST / 解像度: 2.5→15 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: CNS / 分類: refinement | ||||||||||||||||||||
精密化 | *PLUS 最高解像度: 2.5 Å / 最低解像度: 15 Å / σ(F): 2 / Rfactor obs: 0.227 | ||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||
原子変位パラメータ | *PLUS |