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- PDB-1e8g: STRUCTURE OF THE H61T DOUBLE MUTANT OF VANILLYL-ALCOHOL OXIDASE I... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1e8g | ||||||
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Title | STRUCTURE OF THE H61T DOUBLE MUTANT OF VANILLYL-ALCOHOL OXIDASE IN COMPLEX WITH FLUORO-CRESOL | ||||||
![]() | VANILLYL-ALCOHOL OXIDASE | ||||||
![]() | OXIDOREDUCTASE / FLAVOENZYME / SPECIFICITY | ||||||
Function / homology | ![]() vanillyl-alcohol oxidase / vanillyl-alcohol oxidase activity / methanol metabolic process / D-lactate dehydrogenase (cytochrome) activity / lactate catabolic process / D-lactate dehydrogenase activity / FAD binding / peroxisome / mitochondrion Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fraaije, M.W. / Mattevi, A. | ||||||
![]() | ![]() Title: Structural Analysis of Flavinylation in Vanillyl-Alcohol Oxidase Authors: Fraaije, M.W. / Van Der Heuvel, R.H.H. / Van Berkel, W.J.H. / Mattevi, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 238.6 KB | Display | ![]() |
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PDB format | ![]() | 188.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1e8fC ![]() 1e8hC ![]() 1vaoS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.230532, -0.972879, 0.019026), Vector: |
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Components
#1: Protein | Mass: 62969.934 Da / Num. of mol.: 2 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | Compound details | CHAIN A, B ENGINEERED MUTATION HIS61THR CATALYZES THE CONVERSION OF VANILLIN ALCOHOL TO VANILLIN, ...CHAIN A, B ENGINEERED | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 44.92 % | |||||||||||||||
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Crystal grow | pH: 4.6 / Details: pH 4.60 | |||||||||||||||
Crystal grow | *PLUS pH: 5.1 / Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Feb 15, 1999 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→15 Å / Num. obs: 57201 / % possible obs: 98.9 % / Redundancy: 2.5 % / Rmerge(I) obs: 0.06 / Rsym value: 0.06 / Net I/σ(I): 6.2 |
Reflection shell | Resolution: 2.1→2.25 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.297 / Mean I/σ(I) obs: 2.7 / Rsym value: 0.297 / % possible all: 99.7 |
Reflection | *PLUS Num. measured all: 151791 |
Reflection shell | *PLUS % possible obs: 99.7 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1VAO Resolution: 2.1→15 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.1→15 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: p_plane_restr / Dev ideal: 0.009 |