Major capsid protein Gp5 / P22 coat protein - gene protein 5 / viral procapsid / viral procapsid maturation / T=7 icosahedral viral capsid / viral capsid / identical protein binding / Major capsid protein / Major capsid protein
ジャーナル: Proc Natl Acad Sci U S A / 年: 2011 タイトル: Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus. 著者: Dong-Hua Chen / Matthew L Baker / Corey F Hryc / Frank DiMaio / Joanita Jakana / Weimin Wu / Matthew Dougherty / Cameron Haase-Pettingell / Michael F Schmid / Wen Jiang / David Baker / ...著者: Dong-Hua Chen / Matthew L Baker / Corey F Hryc / Frank DiMaio / Joanita Jakana / Weimin Wu / Matthew Dougherty / Cameron Haase-Pettingell / Michael F Schmid / Wen Jiang / David Baker / Jonathan A King / Wah Chiu / 要旨: Formation of many dsDNA viruses begins with the assembly of a procapsid, containing scaffolding proteins and a multisubunit portal but lacking DNA, which matures into an infectious virion. This ...Formation of many dsDNA viruses begins with the assembly of a procapsid, containing scaffolding proteins and a multisubunit portal but lacking DNA, which matures into an infectious virion. This process, conserved among dsDNA viruses such as herpes viruses and bacteriophages, is key to forming infectious virions. Bacteriophage P22 has served as a model system for this study in the past several decades. However, how capsid assembly is initiated, where and how scaffolding proteins bind to coat proteins in the procapsid, and the conformational changes upon capsid maturation still remain elusive. Here, we report Cα backbone models for the P22 procapsid and infectious virion derived from electron cryomicroscopy density maps determined at 3.8- and 4.0-Å resolution, respectively, and the first procapsid structure at subnanometer resolution without imposing symmetry. The procapsid structures show the scaffolding protein interacting electrostatically with the N terminus (N arm) of the coat protein through its C-terminal helix-loop-helix motif, as well as unexpected interactions between 10 scaffolding proteins and the 12-fold portal located at a unique vertex. These suggest a critical role for the scaffolding proteins both in initiating the capsid assembly at the portal vertex and propagating its growth on a T = 7 icosahedral lattice. Comparison of the procapsid and the virion backbone models reveals coordinated and complex conformational changes. These structural observations allow us to propose a more detailed molecular mechanism for the scaffolding-mediated capsid assembly initiation including portal incorporation, release of scaffolding proteins upon DNA packaging, and maturation into infectious virions.
#200 - 2016年8月 正二十面体型ウイルスの準対称性 (Quasisymmetry in Icosahedral Viruses) 類似性 (1)
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マップ
ファイル
ダウンロード / ファイル: emd_1824.map.gz / 形式: CCP4 / 大きさ: 2.3 GB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
This is the icosahedral density map for bacteriophage P22 procapsid solved by cryo-EM at 3.8-Angstrom resolution
ボクセルのサイズ
X=Y=Z: 1.06 Å
密度
表面レベル
登録者による: 0.6 / ムービー #1: 0.6
最小 - 最大
-1.63474905 - 4.04660702
平均 (標準偏差)
0.0003842 (±0.17108497)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-432
-432
-432
サイズ
864
864
864
Spacing
864
864
864
セル
A=B=C: 915.83997 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
1.06
1.06
1.06
M x/y/z
864
864
864
origin x/y/z
0.000
0.000
0.000
length x/y/z
915.840
915.840
915.840
α/β/γ
90.000
90.000
90.000
start NX/NY/NZ
-70
-70
-70
NX/NY/NZ
140
140
140
MAP C/R/S
1
2
3
start NC/NR/NS
-432
-432
-432
NC/NR/NS
864
864
864
D min/max/mean
-1.635
4.047
0.000
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添付データ
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試料の構成要素
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全体 : Bacteriophage P22 procapsid
全体
名称: Bacteriophage P22 procapsid
要素
試料: Bacteriophage P22 procapsid
ウイルス: Enterobacteria phage P22 (ファージ)
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超分子 #1000: Bacteriophage P22 procapsid
超分子
名称: Bacteriophage P22 procapsid / タイプ: sample / ID: 1000 詳細: This sample is wild-type P22 procapsid containing the portal and the scaffolding proteins, but the capsid shell has icosahedral symmetry Number unique components: 1
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超分子 #1: Enterobacteria phage P22
超分子
名称: Enterobacteria phage P22 / タイプ: virus / ID: 1 / Name.synonym: P22 / NCBI-ID: 10754 / 生物種: Enterobacteria phage P22 / ウイルスタイプ: OTHER / ウイルス・単離状態: STRAIN / ウイルス・エンベロープ: No / ウイルス・中空状態: No / Syn species name: P22