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- EMDB-17035: Subsection of a helical nucleocapsid of the Respiratory Syncytial... -
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Open data
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Basic information
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Title | Subsection of a helical nucleocapsid of the Respiratory Syncytial Virus | |||||||||
![]() | Subsection of a helical nucleocapsid of the Respiratory Syncytial Virus. | |||||||||
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![]() | N-RNA / RSV / nucleoprotein / nucleocapsid / VIRAL PROTEIN | |||||||||
Function / homology | ![]() symbiont-mediated suppression of host PKR/eIFalpha signaling / protein serine/threonine kinase inhibitor activity / helical viral capsid / viral nucleocapsid / host cell cytoplasm / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / ribonucleoprotein complex / RNA binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.5 Å | |||||||||
![]() | Gonnin L / Desfosses A / Eleouet JF / Galloux M / Gutsche I | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural landscape of the respiratory syncytial virus nucleocapsids. Authors: Lorène Gonnin / Ambroise Desfosses / Maria Bacia-Verloop / Didier Chevret / Marie Galloux / Jean-François Éléouët / Irina Gutsche / ![]() Abstract: Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and ...Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and a scaffold for the virion assembly. This cryo-electron microscopy analysis reveals the non-canonical arrangement of the HRSV nucleocapsid helix, composed of 16 nucleoproteins per asymmetric unit, and the resulting systematic variations in the RNA accessibility. We demonstrate that this unique helical symmetry originates from longitudinal interactions by the C-terminal arm of the HRSV nucleoprotein. We explore the polymorphism of the nucleocapsid-like assemblies, report five structures of the full-length particles and two alternative arrangements formed by a C-terminally truncated nucleoprotein mutant, and demonstrate the functional importance of the identified longitudinal interfaces. We put all these findings in the context of the HRSV RNA synthesis machinery and delineate the structural basis for its further investigation. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 307 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.8 KB 15.8 KB | Display Display | ![]() |
Images | ![]() | 142.1 KB | ||
Others | ![]() ![]() | 301.7 MB 301.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8op1MC ![]() 8oouC ![]() 8op2C C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Subsection of a helical nucleocapsid of the Respiratory Syncytial Virus. | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.145 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Subsection of a helical nucleocapsid of the Respiratory...
File | emd_17035_half_map_1.map | ||||||||||||
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Annotation | Subsection of a helical nucleocapsid of the Respiratory Syncytial Virus. Half A. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Subsection of a helical nucleocapsid of the Respiratory...
File | emd_17035_half_map_2.map | ||||||||||||
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Annotation | Subsection of a helical nucleocapsid of the Respiratory Syncytial Virus. Half B. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : RSV recombinant nucleocapsid
Entire | Name: RSV recombinant nucleocapsid |
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Components |
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-Supramolecule #1: RSV recombinant nucleocapsid
Supramolecule | Name: RSV recombinant nucleocapsid / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Nucleoprotein
Macromolecule | Name: Nucleoprotein / type: protein_or_peptide / ID: 1 / Number of copies: 5 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 41.971152 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: ALSKVKLNDT LNKDQLLSSS KYTIQRSTGD SIDTPNYDVQ KHINKLCGML LITEDANHKF TGLIGMLYAM SRLGREDTIK ILRDAGYHV KANGVDVTTH RQDINGKEMK FEVLTLASLT TEIQINIEIE SRKSYKKMLK EMGEVAPEYR HDSPDCGMII L CIAALVIT ...String: ALSKVKLNDT LNKDQLLSSS KYTIQRSTGD SIDTPNYDVQ KHINKLCGML LITEDANHKF TGLIGMLYAM SRLGREDTIK ILRDAGYHV KANGVDVTTH RQDINGKEMK FEVLTLASLT TEIQINIEIE SRKSYKKMLK EMGEVAPEYR HDSPDCGMII L CIAALVIT KLAAGDRSGL TAVIRRANNV LKNEMKRYKG LLPKDIANSF YEVFEKHPHF IDVFVHFGIA QSSTRGGSRV EG IFAGLFM NAYGAGQVML RWGVLAKSVK NIMLGHASVQ AEMEQVVEVY EYAQKLGGEA GFYHILNNPK ASLLSLTQFP HFS SVVLGN AAGLGIMGEY RGTPRNQDLY DAAKAYAEQL KENGVINYSV LDLTAEELEA IK UniProtKB: Nucleoprotein |
-Macromolecule #2: RNA (5'-R(P*CP*CP*CP*CP*CP*CP*C)-3')
Macromolecule | Name: RNA (5'-R(P*CP*CP*CP*CP*CP*CP*C)-3') / type: rna / ID: 2 / Number of copies: 5 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 2.091315 KDa |
Sequence | String: CCCCCCC |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | filament |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | TFS GLACIOS |
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Image recording | Film or detector model: GATAN K2 BASE (4k x 4k) / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 0.9 µm |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 389540 |
Initial angle assignment | Type: PROJECTION MATCHING |
Final angle assignment | Type: PROJECTION MATCHING |