Journal: Nat Commun / Year: 2023 Title: Structural landscape of the respiratory syncytial virus nucleocapsids. Authors: Lorène Gonnin / Ambroise Desfosses / Maria Bacia-Verloop / Didier Chevret / Marie Galloux / Jean-François Éléouët / Irina Gutsche / Abstract: Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and ...Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and a scaffold for the virion assembly. This cryo-electron microscopy analysis reveals the non-canonical arrangement of the HRSV nucleocapsid helix, composed of 16 nucleoproteins per asymmetric unit, and the resulting systematic variations in the RNA accessibility. We demonstrate that this unique helical symmetry originates from longitudinal interactions by the C-terminal arm of the HRSV nucleoprotein. We explore the polymorphism of the nucleocapsid-like assemblies, report five structures of the full-length particles and two alternative arrangements formed by a C-terminally truncated nucleoprotein mutant, and demonstrate the functional importance of the identified longitudinal interfaces. We put all these findings in the context of the HRSV RNA synthesis machinery and delineate the structural basis for its further investigation.
Entire : Double ring nucleocapsid of the human Respiratory Syncytial Virus
Entire
Name: Double ring nucleocapsid of the human Respiratory Syncytial Virus
Components
Complex: Double ring nucleocapsid of the human Respiratory Syncytial Virus
Protein or peptide: Nucleoprotein
RNA: RNA (70-mer)
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Supramolecule #1: Double ring nucleocapsid of the human Respiratory Syncytial Virus
Supramolecule
Name: Double ring nucleocapsid of the human Respiratory Syncytial Virus type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 Details: Double ring nucleocapsid formed by the nucleoprotein N of the human RSV upon overexpression in insect cells and encapsidation of cellular RNA
Source (natural)
Organism: Human respiratory syncytial virus A strain Long
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Macromolecule #1: Nucleoprotein
Macromolecule
Name: Nucleoprotein / type: protein_or_peptide / ID: 1 / Number of copies: 20 / Enantiomer: LEVO
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