ジャーナル: Nat Commun / 年: 2023 タイトル: Structural landscape of the respiratory syncytial virus nucleocapsids. 著者: Lorène Gonnin / Ambroise Desfosses / Maria Bacia-Verloop / Didier Chevret / Marie Galloux / Jean-François Éléouët / Irina Gutsche / 要旨: Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and ...Human Respiratory Syncytial Virus (HRSV) is a prevalent cause of severe respiratory infections in children and the elderly. The helical HRSV nucleocapsid is a template for the viral RNA synthesis and a scaffold for the virion assembly. This cryo-electron microscopy analysis reveals the non-canonical arrangement of the HRSV nucleocapsid helix, composed of 16 nucleoproteins per asymmetric unit, and the resulting systematic variations in the RNA accessibility. We demonstrate that this unique helical symmetry originates from longitudinal interactions by the C-terminal arm of the HRSV nucleoprotein. We explore the polymorphism of the nucleocapsid-like assemblies, report five structures of the full-length particles and two alternative arrangements formed by a C-terminally truncated nucleoprotein mutant, and demonstrate the functional importance of the identified longitudinal interfaces. We put all these findings in the context of the HRSV RNA synthesis machinery and delineate the structural basis for its further investigation.
全体 : Helical nucleocapsid of the human Respiratory Syncytial Virus
全体
名称: Helical nucleocapsid of the human Respiratory Syncytial Virus
要素
複合体: Helical nucleocapsid of the human Respiratory Syncytial Virus
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超分子 #1: Helical nucleocapsid of the human Respiratory Syncytial Virus
超分子
名称: Helical nucleocapsid of the human Respiratory Syncytial Virus タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#2 詳細: Helical nucleocapsid with a non-canonical symmetry formed by the nucleoprotein N of the human RSV upon overexpression in insect cells and encapsidation of cellular RNA
由来(天然)
生物種: Human respiratory syncytial virus A strain Long (ウイルス)
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実験情報
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構造解析
手法
クライオ電子顕微鏡法
解析
らせん対称体再構成法
試料の集合状態
particle
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試料調製
緩衝液
pH: 7.4
凍結
凍結剤: ETHANE
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電子顕微鏡法
顕微鏡
TFS GLACIOS
撮影
フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 42.0 e/Å2