- EMDB-16837: Structure of the mammalian Pol II-SPT6-Elongin complex, lacking E... -
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基本情報
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データベース: EMDB / ID: EMD-16837
タイトル
Structure of the mammalian Pol II-SPT6-Elongin complex, lacking ELOA latch (composite map 2)
マップデータ
composite map for Pol II-SPT6-Elongin (NoN) model
試料
複合体: The Pol II-SPT6-Elongin transcription elongation complex
タンパク質・ペプチド: x 16種
DNA: x 2種
RNA: x 1種
リガンド: x 2種
キーワード
Transcription elongation / Elongin / RNA polymerase II / TRANSCRIPTION
機能・相同性
機能・相同性情報
regulation of isotype switching / regulation of mRNA export from nucleus / regulation of muscle cell differentiation / nucleosome organization / regulation of mRNA processing / B-WICH complex positively regulates rRNA expression / RNA Polymerase I Transcription Initiation / RNA Polymerase I Promoter Escape / RNA Polymerase I Transcription Termination / RNA Polymerase III Transcription Initiation From Type 1 Promoter ...regulation of isotype switching / regulation of mRNA export from nucleus / regulation of muscle cell differentiation / nucleosome organization / regulation of mRNA processing / B-WICH complex positively regulates rRNA expression / RNA Polymerase I Transcription Initiation / RNA Polymerase I Promoter Escape / RNA Polymerase I Transcription Termination / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / Formation of RNA Pol II elongation complex / Formation of the Early Elongation Complex / Transcriptional regulation by small RNAs / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / FGFR2 alternative splicing / RNA polymerase II transcribes snRNA genes / mRNA Capping / mRNA Splicing - Major Pathway / mRNA Splicing - Minor Pathway / Processing of Capped Intron-Containing Pre-mRNA / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Elongation / RNA Polymerase II Transcription Initiation And Promoter Clearance / RNA Pol II CTD phosphorylation and interaction with CE / Estrogen-dependent gene expression / Formation of TC-NER Pre-Incision Complex / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / target-directed miRNA degradation / elongin complex / blastocyst formation / VCB complex / Cul5-RING ubiquitin ligase complex / Cul2-RING ubiquitin ligase complex / transcription elongation-coupled chromatin remodeling / : / : / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / site of DNA damage / organelle membrane / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / transcription elongation by RNA polymerase I / Tat-mediated elongation of the HIV-1 transcript / mRNA transport / positive regulation of translational initiation / Formation of HIV-1 elongation complex containing HIV-1 Tat / RNA polymerase I complex / RNA polymerase III complex / transcription-coupled nucleotide-excision repair / Formation of HIV elongation complex in the absence of HIV Tat / RNA polymerase III activity / tRNA transcription by RNA polymerase III / RNA polymerase II, core complex / RNA polymerase I activity / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / RNA polymerase II activity / nucleosome binding / translation initiation factor binding / RNA Polymerase II Pre-transcription Events / RNA splicing / positive regulation of RNA splicing / transcription corepressor binding / transcription elongation factor complex / transcription elongation by RNA polymerase II / promoter-specific chromatin binding / transcription initiation at RNA polymerase II promoter / TP53 Regulates Transcription of DNA Repair Genes / positive regulation of transcription elongation by RNA polymerase II / Vif-mediated degradation of APOBEC3G / P-body / Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha / Inactivation of CSF3 (G-CSF) signaling / Evasion by RSV of host interferon responses / mRNA processing / ribonucleoside binding / fibrillar center / Regulation of expression of SLITs and ROBOs / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / Antigen processing: Ubiquitination & Proteasome degradation / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / protein-macromolecule adaptor activity / Neddylation / chromosome / single-stranded DNA binding / 加水分解酵素; エステル加水分解酵素; 5'-リン酸モノエステル産生エキソリボヌクレアーゼ / histone binding / ubiquitin-dependent protein catabolic process / protein-containing complex assembly / transcription by RNA polymerase II 類似検索 - 分子機能
RNA polymerase II subunit D / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase subunit beta / RNA polymerase II, I and III subunit K / DNA-directed RNA polymerase II subunit E ...RNA polymerase II subunit D / DNA-directed RNA polymerase subunit / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / DNA-directed RNA polymerase II subunit RPB11-a / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerase subunit beta / RNA polymerase II, I and III subunit K / DNA-directed RNA polymerase II subunit E / DNA-directed RNA polymerase II subunit RPB1 / DNA-directed RNA polymerase II subunit RPB9 / Elongin-A / Elongin-C / Elongin-B / Transcription elongation factor SPT6 類似検索 - 構成要素
生物種
Homo sapiens (ヒト) / Sus scrofa (ブタ) / synthetic construct (人工物)
ジャーナル: Nat Struct Mol Biol / 年: 2023 タイトル: Structure of the transcribing RNA polymerase II-Elongin complex. 著者: Ying Chen / Goran Kokic / Christian Dienemann / Olexandr Dybkov / Henning Urlaub / Patrick Cramer / 要旨: Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to ...Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongin subunit ELOA binds the RPB2 side of Pol II and anchors the ELOB-ELOC subunit heterodimer. ELOA contains a 'latch' that binds between the end of the Pol II bridge helix and funnel helices, thereby inducing a conformational change near the polymerase active center. The latch is required for the elongation-stimulatory activity of Elongin, but not for Pol II binding, indicating that Elongin functions by allosterically regulating the conformational mobility of the polymerase active center. Elongin binding to Pol II is incompatible with association of the super elongation complex, PAF1 complex and RTF1, which also contain an elongation-stimulatory latch element.
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB3
タンパク質・ペプチド: RNA polymerase II subunit D
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit E
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit F
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB7
タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC3
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB9
タンパク質・ペプチド: DNA-directed RNA polymerases I, II, and III subunit RPABC5
タンパク質・ペプチド: DNA-directed RNA polymerase II subunit RPB11-a
タンパク質・ペプチド: RNA polymerase II subunit K
DNA: Non-Template DNA
RNA: RNA
DNA: Template DNA
タンパク質・ペプチド: Elongin-A
タンパク質・ペプチド: Elongin-C
タンパク質・ペプチド: Elongin-B
タンパク質・ペプチド: Transcription elongation factor SPT6
リガンド: MAGNESIUM ION
リガンド: ZINC ION
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超分子 #1: The Pol II-SPT6-Elongin transcription elongation complex
超分子
名称: The Pol II-SPT6-Elongin transcription elongation complex タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#19 詳細: The map is generated from a sub-class of a Cryo-EM dataset for the Pol II-SPT6-Elongin complex.
由来(天然)
生物種: Homo sapiens (ヒト)
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分子 #1: DNA-directed RNA polymerase II subunit RPB1