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基本情報
登録情報 | データベース: EMDB / ID: EMD-1669 | |||||||||
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タイトル | Cryo-EM structures of the idle yeast Ssh1 complex bound to the yeast 80S ribosome | |||||||||
![]() | This map represents a yeast 80S ribosome bound to the idle yeast Ssh1 complex | |||||||||
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![]() | Ribosome / protein exit tunnel / cotranslational protein translocation / protein conducting channel / signal sequence | |||||||||
機能・相同性 | ![]() : / translocon complex / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / rough endoplasmic reticulum membrane / Ssh1 translocon complex / Sec61 translocon complex / SRP-dependent cotranslational protein targeting to membrane / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / signal sequence binding ...: / translocon complex / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / rough endoplasmic reticulum membrane / Ssh1 translocon complex / Sec61 translocon complex / SRP-dependent cotranslational protein targeting to membrane / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / signal sequence binding / positive regulation of translational fidelity / Protein methylation / post-translational protein targeting to membrane, translocation / RMTs methylate histone arginines / Protein hydroxylation / SRP-dependent cotranslational protein targeting to membrane, translocation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / nuclear inner membrane / pre-mRNA 5'-splice site binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translational elongation / preribosome, small subunit precursor / response to cycloheximide / mRNA destabilization / Major pathway of rRNA processing in the nucleolus and cytosol / protein kinase activator activity / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / L13a-mediated translational silencing of Ceruloplasmin expression / preribosome, large subunit precursor / ribosomal large subunit export from nucleus / protein transmembrane transporter activity / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / G-protein alpha-subunit binding / regulation of translational fidelity / protein-RNA complex assembly / ribosomal small subunit export from nucleus / translation regulator activity / ribonucleoprotein complex binding / ribosomal subunit export from nucleus / translational termination / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / DNA-(apurinic or apyrimidinic site) endonuclease activity / rescue of stalled ribosome / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / guanyl-nucleotide exchange factor activity / maturation of LSU-rRNA / ribosomal large subunit biogenesis / maturation of SSU-rRNA / macroautophagy / small-subunit processome / positive regulation of apoptotic signaling pathway / protein kinase C binding / maintenance of translational fidelity / ribosomal large subunit assembly / modification-dependent protein catabolic process / cytoplasmic stress granule / rRNA processing / protein tag activity / large ribosomal subunit / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / protein ubiquitination / structural constituent of ribosome / translation / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / endoplasmic reticulum membrane / nucleolus / structural molecule activity / endoplasmic reticulum / mitochondrion / RNA binding 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / ネガティブ染色法 / 解像度: 8.8 Å | |||||||||
![]() | Becker T / Mandon E / Bhushan S / Jarasch A / Armache JP / Funes S / Jossinet F / Gumbart J / Mielke T / Berninghausen O ...Becker T / Mandon E / Bhushan S / Jarasch A / Armache JP / Funes S / Jossinet F / Gumbart J / Mielke T / Berninghausen O / Schulten K / Westhof E / Gilmore R / Beckmann R | |||||||||
![]() | ![]() タイトル: Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome. 著者: Thomas Becker / Shashi Bhushan / Alexander Jarasch / Jean-Paul Armache / Soledad Funes / Fabrice Jossinet / James Gumbart / Thorsten Mielke / Otto Berninghausen / Klaus Schulten / Eric ...著者: Thomas Becker / Shashi Bhushan / Alexander Jarasch / Jean-Paul Armache / Soledad Funes / Fabrice Jossinet / James Gumbart / Thorsten Mielke / Otto Berninghausen / Klaus Schulten / Eric Westhof / Reid Gilmore / Elisabet C Mandon / Roland Beckmann / ![]() 要旨: The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may ...The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. In combination with biochemical data, we found that in both idle and active states, the Sec complex is not oligomeric and interacts mainly via two cytoplasmic loops with the universal ribosomal adaptor site. In the active state, the ribosomal tunnel and a central pore of the monomeric PCC were occupied by the nascent chain, contacting loop 6 of the Sec complex. This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation. | |||||||||
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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マップデータ | ![]() | 21.5 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 12.4 KB 12.4 KB | 表示 表示 | ![]() |
画像 | ![]() ![]() | 91.2 KB 220.3 KB | ||
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文書・要旨 | ![]() | 356.4 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 356 KB | 表示 | |
XML形式データ | ![]() | 7.2 KB | 表示 | |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | This map represents a yeast 80S ribosome bound to the idle yeast Ssh1 complex | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.2375 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
-全体 : An idle yeast Ssh1 complex bound to a yeast 80S ribosome
全体 | 名称: An idle yeast Ssh1 complex bound to a yeast 80S ribosome |
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要素 |
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-超分子 #1000: An idle yeast Ssh1 complex bound to a yeast 80S ribosome
超分子 | 名称: An idle yeast Ssh1 complex bound to a yeast 80S ribosome タイプ: sample / ID: 1000 詳細: 80S ribosomes and the detergent solubilized Ssh1 complex were reconstituted in vitro by adding 1 pmol of ribosome and Ssh1 complex in 5 fold molar excess 集合状態: 80S Ribosome bound to one copy of the heterotrimeric Ssh1 complex Number unique components: 2 |
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分子量 | 実験値: 4.2 MDa / 理論値: 4.2 MDa / 手法: Known for 80S ribosomes |
-超分子 #1: Yeast 80S ribosome bound to the yeast Ssh1 complex
超分子 | 名称: Yeast 80S ribosome bound to the yeast Ssh1 complex / タイプ: complex / ID: 1 Name.synonym: Yeast 80S ribosome bound to the yeast Ssh1 complex Ribosome-details: ribosome-eukaryote: ALL |
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分子量 | 実験値: 4.2 MDa / 理論値: 4.2 MDa |
-分子 #1: Ssh1 complex
分子 | 名称: Ssh1 complex / タイプ: ligand / ID: 1 / Name.synonym: Ssh1 complex / 詳細: His FLAG-tagged / コピー数: 1 / 集合状態: Heterotrimer / 組換発現: Yes |
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由来(天然) | 生物種: ![]() ![]() |
分子量 | 理論値: 71.5 KDa |
組換発現 | 生物種: ![]() ![]() |
-実験情報
-構造解析
手法 | ネガティブ染色法, クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 詳細: 20 mM HEPES/KOH, pH 7.5 100 mM KOAc, 10 mM Mg(OAc)2, 1.5 mM DTT, 0.1 % (w/v) digitonin |
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染色 | タイプ: NEGATIVE / 詳細: Cryo-EM |
グリッド | 詳細: Quantifoil grids (3/3) with 2 nm carbon on top |
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 95 % / 装置: OTHER / 詳細: Vitrification instrument: Vitrobot 手法: Blot for 10 seconds before plunging, use 2 layer of filter paper |
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電子顕微鏡法
顕微鏡 | FEI POLARA 300 |
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温度 | 平均: 84 K |
アライメント法 | Legacy - 非点収差: Objective lens astigmatism was corrected at 100000 times magnification |
撮影 | カテゴリ: FILM / フィルム・検出器のモデル: KODAK SO-163 FILM / デジタル化 - スキャナー: PRIMESCAN / デジタル化 - サンプリング間隔: 4.76 µm / 実像数: 185 / 平均電子線量: 25 e/Å2 / 詳細: Scanned at 5334 dpi / Od range: 1.2 / ビット/ピクセル: 16 |
Tilt angle min | 0 |
Tilt angle max | 0 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 倍率(補正後): 38000 / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.26 mm / 最大 デフォーカス(公称値): 4.5 µm / 最小 デフォーカス(公称値): 1.2 µm / 倍率(公称値): 39000 |
試料ステージ | 試料ホルダー: FEI Polara cartridge system / 試料ホルダーモデル: OTHER |
実験機器 | ![]() モデル: Tecnai Polara / 画像提供: FEI Company |
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画像解析
詳細 | Particles were selected using the program SIGNATURE and visually inspected. This map resulted from sorting against the ES27 exit position and subsequent sorting for tRNA and the Ssh1 complex and represents the datasubset with the Ssh1 complex present without tRNA. |
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CTF補正 | 詳細: Defocus group volumes |
最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / アルゴリズム: OTHER / 解像度のタイプ: BY AUTHOR / 解像度: 8.8 Å / 解像度の算出法: FSC 0.5 CUT-OFF / ソフトウェア - 名称: SPIDER 詳細: Map was filtered between 8.3 and 10.3 Angstrom to better visualize the Ssh1 complex 使用した粒子像数: 20400 |
最終 角度割当 | 詳細: SPIDER |