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Yorodumi- EMDB-13638: Structure of pentameric S-layer protein from Halofaerax volcanii -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-13638 | ||||||||||||
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Title | Structure of pentameric S-layer protein from Halofaerax volcanii | ||||||||||||
Map data | Non-postprocessed, non-sharpened full map. | ||||||||||||
Sample |
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Function / homology | Surface glycoprotein signal peptide / Major cell surface glycoprotein / PGF-CTERM archaeal protein-sorting signal / PGF-CTERM motif / S-layer / cell wall organization / extracellular region / plasma membrane / Cell surface glycoprotein Function and homology information | ||||||||||||
Biological species | Haloferax volcanii DS2 (archaea) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.87 Å | ||||||||||||
Authors | von Kuegelgen A / Bharat TAM | ||||||||||||
Funding support | United Kingdom, 3 items
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Citation | Journal: Cell Rep / Year: 2021 Title: Complete atomic structure of a native archaeal cell surface. Authors: Andriko von Kügelgen / Vikram Alva / Tanmay A M Bharat / Abstract: Many prokaryotic cells are covered by an ordered, proteinaceous, sheet-like structure called a surface layer (S-layer). S-layer proteins (SLPs) are usually the highest copy number macromolecules in ...Many prokaryotic cells are covered by an ordered, proteinaceous, sheet-like structure called a surface layer (S-layer). S-layer proteins (SLPs) are usually the highest copy number macromolecules in prokaryotes, playing critical roles in cellular physiology such as blocking predators, scaffolding membranes, and facilitating environmental interactions. Using electron cryomicroscopy of two-dimensional sheets, we report the atomic structure of the S-layer from the archaeal model organism Haloferax volcanii. This S-layer consists of a hexagonal array of tightly interacting immunoglobulin-like domains, which are also found in SLPs across several classes of archaea. Cellular tomography reveal that the S-layer is nearly continuous on the cell surface, completed by pentameric defects in the hexagonal lattice. We further report the atomic structure of the SLP pentamer, which shows markedly different relative arrangements of SLP domains needed to complete the S-layer. Our structural data provide a framework for understanding cell surfaces of archaea at the atomic level. | ||||||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_13638.map.gz | 96.8 MB | EMDB map data format | |
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Header (meta data) | emd-13638-v30.xml emd-13638.xml | 26.4 KB 26.4 KB | Display Display | EMDB header |
Images | emd_13638.png | 140.9 KB | ||
Masks | emd_13638_msk_1.map | 125 MB | Mask map | |
Others | emd_13638_additional_1.map.gz emd_13638_half_map_1.map.gz emd_13638_half_map_2.map.gz | 116.9 MB 98 MB 98 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-13638 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-13638 | HTTPS FTP |
-Related structure data
Related structure data | 7ptuMC 7ptpC 7ptrC 7pttC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_13638.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Non-postprocessed, non-sharpened full map. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | emd_13638_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: Postprocessed, sharpened map with an applied B-factor of -170 A^2
File | emd_13638_additional_1.map | ||||||||||||
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Annotation | Postprocessed, sharpened map with an applied B-factor of -170 A^2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 1, non-postprocessed, non sharpened.
File | emd_13638_half_map_1.map | ||||||||||||
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Annotation | Half map 1, non-postprocessed, non sharpened. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map 2, non-postprocessed, non sharpened.
File | emd_13638_half_map_2.map | ||||||||||||
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Annotation | Half map 2, non-postprocessed, non sharpened. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Structure of pentameric S-layer protein csg
Entire | Name: Structure of pentameric S-layer protein csg |
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Components |
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-Supramolecule #1: Structure of pentameric S-layer protein csg
Supramolecule | Name: Structure of pentameric S-layer protein csg / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 / Details: Structure of pentameric S-layer protein csg |
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Source (natural) | Organism: Haloferax volcanii DS2 (archaea) / Location in cell: Cell surface |
-Macromolecule #1: Cell surface glycoprotein
Macromolecule | Name: Cell surface glycoprotein / type: protein_or_peptide / ID: 1 / Number of copies: 5 / Enantiomer: LEVO |
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Source (natural) | Organism: Haloferax volcanii DS2 (archaea) |
Molecular weight | Theoretical: 81.755602 KDa |
Sequence | String: ERGNLDADSE SFNKTIQSGD RVFLGEEIST DAGLGASNPL LTGTAGNSEG VSLDLSSPIP QTTENQPLGT YDVDGSGSAT TPNVTLLAP RITDSEILTS SGGDVTGSAI SSSDAGNLYV NADYNYESAE KVEVTVEDPS GTDITNEVLS GTDTFVDDGS I GSTSSTGG ...String: ERGNLDADSE SFNKTIQSGD RVFLGEEIST DAGLGASNPL LTGTAGNSEG VSLDLSSPIP QTTENQPLGT YDVDGSGSAT TPNVTLLAP RITDSEILTS SGGDVTGSAI SSSDAGNLYV NADYNYESAE KVEVTVEDPS GTDITNEVLS GTDTFVDDGS I GSTSSTGG GVGIDMSDQD AGEYTIILEG AEDLDFGDAT ETMTLTISSQ DEIGIELDSE SVTQGTDVQY TVTNGIDGNE HV VAMDLSD LQNDATTEQA KEVFRNIGDT SEVGIANSSA TNTSGSSTGP TVETADIAYA VVEIDGASAV GGIETQYLDD SEV DLEVYD AGVSATAAVG QDATNDITLT IEEGGTTLSS PTGQYVVGSE VDINGTATSS DSVAIYVRDD GDWQLLEIGG DNEI SVDSD DTFEEEDIAL SGLSGDGSSI LSLTGTYRIG VIDASDADVG GDGSVDDSLT TSEFTSGVSS SNSIRVTDQA LTGQF TTIN GQVAPVETGT VDINGTASGA NSVLVIFVDE RGNVNYQEVS VDSDGTYDED DITVGLTQGR VTAHILSVGR DSAIGD GSL PSGPSNGATL NDLTGYLDTL DQNNNNGEQI NELIASETVD ETASDDLIVT ETFRLAESST SIDSIYPDAA EAAGINP VA TGETMVIAGS TNLKPDDNTI SIEVTNEDGT SVALEDTDEW NNDGQWMVEI DTTDFETGTF TVEADDGDNT DTVNVEVV S EREDTTTSSD NATDTTTTTD GPTETTTTAE PTETTEEPTE ETTTSSNTPG FGIAVALVAL VGAALLALRR EN |
-Macromolecule #2: beta-D-glucopyranose
Macromolecule | Name: beta-D-glucopyranose / type: ligand / ID: 2 / Number of copies: 5 / Formula: BGC |
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Molecular weight | Theoretical: 180.156 Da |
Chemical component information | ChemComp-BGC: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 2.5 mg/mL | |||||||||||||||
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Buffer | pH: 7.5 Component:
Details: Buffer solutions were prepared fresh from sterile filtered concentrated stocksolutions. Solutions were filtered through a 0.22 um filter to avoid microbial contamination and degassed using a ...Details: Buffer solutions were prepared fresh from sterile filtered concentrated stocksolutions. Solutions were filtered through a 0.22 um filter to avoid microbial contamination and degassed using a vacuum fold pump. The pH of the HEPES stock solution was adjusted with sodium hydroxide at 4 deg C. 1.75 mM holmium chloride was added 2 hours before vitrification. | |||||||||||||||
Grid | Model: Quantifoil R2/2 / Material: COPPER/RHODIUM / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Details: 20 seconds, 15 mA | |||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 283.15 K / Instrument: FEI VITROBOT MARK IV Details: Vitrobot options: Blot time 5 seconds, Blot force -10,1, Wait time 10 seconds, Drain time 0.5 seconds. | |||||||||||||||
Details | Purified csg protein mixed with 1.75 mM HoCl3 after 2 hour incubation. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Temperature | Min: 70.0 K / Max: 70.0 K |
Specialist optics | Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV |
Details | EPU software with faster acquisition mode AFIS (Aberration Free Image Shift). |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number grids imaged: 2 / Number real images: 11871 / Average exposure time: 3.6 sec. / Average electron dose: 53.9 e/Å2 Details: Images were collected in two sessions in movie-mode and subjected to 3.6 seconds of exposure where a total dose of 53.45 or 53.9 e-/A2 was applied, and 40 frames were recorded per movie. A ...Details: Images were collected in two sessions in movie-mode and subjected to 3.6 seconds of exposure where a total dose of 53.45 or 53.9 e-/A2 was applied, and 40 frames were recorded per movie. A total of 11871 movies were collected in two sessions with the same microscope and settings. |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 50.0 µm / Calibrated defocus max: 4.0 µm / Calibrated defocus min: 1.0 µm / Calibrated magnification: 81000 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 81000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
+Image processing
-Atomic model buiding 1
Details | The initial manual build of D1-D2 was performed independently using the csg pentameric cryo-EM map, which served as an additional validation of the manual building performed in the csg hexamer in the related deposition. The manual building exercise yielded a nearly identical result to the hexamer; thus, the final refined hexameric structures of D1-D2, along with D3-D4 were taken and fitted into the pentameric map (~3.87 angstrom resolution in D1-D3, lower in D4 which is partially resolved). Five copies of these D1-D4 were used for refinement and model building as for the hexamer, except D3 and D4 was restrained in position, due to steadily deteriorating resolution in this part of the map. D5-D6 were not resolved in the pentameric structure and were thus not included in the refinements. Model validation was performed in PHENIX and CCP-EM. |
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Refinement | Space: REAL / Protocol: AB INITIO MODEL / Overall B value: 179.68 / Target criteria: Best Fit |
Output model | PDB-7ptu: |