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Yorodumi- EMDB-12912: Nog1-TAP associated immature ribosomal particles from S. cerevisi... -
+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-12912 | |||||||||
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Title | Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population A | |||||||||
Map data | Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population A, full map | |||||||||
Sample |
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Keywords | ribosomal assembly state / RIBOSOME | |||||||||
Function / homology | Function and homology information snoRNA release from pre-rRNA / exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / PeBoW complex / ATP-dependent activity, acting on RNA / rRNA primary transcript binding / positive regulation of ATP-dependent activity / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / proteasome binding ...snoRNA release from pre-rRNA / exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / PeBoW complex / ATP-dependent activity, acting on RNA / rRNA primary transcript binding / positive regulation of ATP-dependent activity / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / proteasome binding / maturation of 5.8S rRNA / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / ribosomal large subunit binding / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / nuclear-transcribed mRNA catabolic process / ATPase activator activity / L13a-mediated translational silencing of Ceruloplasmin expression / preribosome, large subunit precursor / translational elongation / ribosomal large subunit export from nucleus / 90S preribosome / ribosomal subunit export from nucleus / ribonucleoprotein complex binding / translation initiation factor activity / maturation of LSU-rRNA / proteasome complex / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / assembly of large subunit precursor of preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cytosolic ribosome assembly / ribosomal large subunit biogenesis / maturation of SSU-rRNA / small-subunit processome / macroautophagy / maintenance of translational fidelity / protein catabolic process / rRNA processing / nuclear envelope / ribosomal small subunit biogenesis / protein-macromolecule adaptor activity / ATPase binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / RNA helicase activity / rRNA binding / negative regulation of translation / ribosome / RNA helicase / structural constituent of ribosome / translation / GTPase activity / mRNA binding / nucleolus / GTP binding / ATP hydrolysis activity / DNA binding / RNA binding / nucleoplasm / ATP binding / identical protein binding / nucleus / metal ion binding / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | Saccharomyces cerevisiae S288C (yeast) / Saccharomyces cerevisiae S288c (yeast) / Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.3 Å | |||||||||
Authors | Milkereit P / Poell G | |||||||||
Funding support | Germany, 1 items
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Citation | Journal: PLoS One / Year: 2021 Title: Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors. Authors: Gisela Pöll / Michael Pilsl / Joachim Griesenbeck / Herbert Tschochner / Philipp Milkereit / Abstract: In yeast and human cells many of the ribosomal proteins (r-proteins) are required for the stabilisation and productive processing of rRNA precursors. Functional coupling of r-protein assembly with ...In yeast and human cells many of the ribosomal proteins (r-proteins) are required for the stabilisation and productive processing of rRNA precursors. Functional coupling of r-protein assembly with the stabilisation and maturation of subunit precursors potentially promotes the production of ribosomes with defined composition. To further decipher mechanisms of such an intrinsic quality control pathway we analysed here the contribution of three yeast large ribosomal subunit r-proteins rpL2 (uL2), rpL25 (uL23) and rpL34 (eL34) for intermediate nuclear subunit folding steps. Structure models obtained from single particle cryo-electron microscopy analyses provided evidence for specific and hierarchic effects on the stable positioning and remodelling of large ribosomal subunit domains. Based on these structural and previous biochemical data we discuss possible mechanisms of r-protein dependent hierarchic domain arrangement and the resulting impact on the stability of misassembled subunits. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_12912.map.gz | 192.8 MB | EMDB map data format | |
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Header (meta data) | emd-12912-v30.xml emd-12912.xml | 63.3 KB 63.3 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_12912_fsc.xml | 14.2 KB | Display | FSC data file |
Images | emd_12912.png | 105 KB | ||
Filedesc metadata | emd-12912.cif.gz | 15 KB | ||
Others | emd_12912_half_map_1.map.gz emd_12912_half_map_2.map.gz | 193.2 MB 193.2 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-12912 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-12912 | HTTPS FTP |
-Related structure data
Related structure data | 7ohxMC 7of1C 7oh3C 7ohpC 7ohqC 7ohrC 7ohsC 7ohtC 7ohuC 7ohvC 7ohwC 7ohyC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | |
EM raw data | EMPIAR-10775 (Title: Nog1-TAP associated immature ribosomal particles from S. cerevisiae depleted of rpL34 Data size: 6.2 TB Data #1: Unaligned multi-frame migrographs of Nog1-TAP associated immature ribosomes depleted of rpL34 [micrographs - multiframe]) |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_12912.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population A, full map | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.0635 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Half map: Nog1-TAP associated immature ribosomal particles from S. cerevisiae...
File | emd_12912_half_map_1.map | ||||||||||||
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Annotation | Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population A, half map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Nog1-TAP associated immature ribosomal particles from S. cerevisiae...
File | emd_12912_half_map_2.map | ||||||||||||
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Annotation | Nog1-TAP associated immature ribosomal particles from S. cerevisiae after rpL34 expression shut down, population A, half map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Nog1-TAP associated immature ribosomal particles from cells deple...
+Supramolecule #1: Nog1-TAP associated immature ribosomal particles from cells deple...
+Macromolecule #1: 25S rRNA
+Macromolecule #2: 5.8S rRNA
+Macromolecule #6: ITS2
+Macromolecule #3: Protein MAK16
+Macromolecule #4: Ribosomal RNA-processing protein 1
+Macromolecule #5: Ribosome biogenesis protein NSA1
+Macromolecule #7: 60S ribosomal protein L3
+Macromolecule #8: 60S ribosomal protein L4-A
+Macromolecule #9: ATP-dependent RNA helicase HAS1
+Macromolecule #10: 60S ribosomal protein L6-A
+Macromolecule #11: 60S ribosomal protein L7-A
+Macromolecule #12: 60S ribosomal protein L8-A
+Macromolecule #13: 60S ribosomal protein L9-A
+Macromolecule #14: Proteasome-interacting protein CIC1
+Macromolecule #15: 60S ribosomal protein L13-A
+Macromolecule #16: 60S ribosomal protein L14-A
+Macromolecule #17: 60S ribosomal protein L15-A
+Macromolecule #18: 60S ribosomal protein L16-A
+Macromolecule #19: 60S ribosomal protein L17-A
+Macromolecule #20: 60S ribosomal protein L18-A
+Macromolecule #21: 60S ribosomal protein L20-A
+Macromolecule #22: Ribosome assembly factor MRT4
+Macromolecule #23: 60S ribosomal protein L26-A
+Macromolecule #24: Nucleolar GTP-binding protein 1
+Macromolecule #25: 60S ribosomal protein L32
+Macromolecule #26: 60S ribosomal protein L33-A
+Macromolecule #27: 60S ribosomal protein L35-A
+Macromolecule #28: 60S ribosomal protein L36-A
+Macromolecule #29: 60S ribosomal protein L37-A
+Macromolecule #30: Ribosome biogenesis protein ERB1
+Macromolecule #31: Pescadillo homolog
+Macromolecule #32: Ribosome biogenesis protein 15
+Macromolecule #33: Ribosome biogenesis protein RLP7
+Macromolecule #34: Ribosome biogenesis protein RLP24
+Macromolecule #35: Nucleolar protein 16
+Macromolecule #36: Ribosome production factor 1
+Macromolecule #37: Eukaryotic translation initiation factor 6
+Macromolecule #38: ZINC ION
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 Component:
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Grid | Model: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 200 sec. / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.4 kPa | ||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average exposure time: 5.16 sec. / Average electron dose: 88.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |