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データを開く
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基本情報
登録情報 | データベース: EMDB / ID: EMD-12799 | |||||||||
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タイトル | SRP-SR at the distal site conformation | |||||||||
![]() | SRP-SR complex from 3D focused refinement on ribosome signal subtracted images | |||||||||
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![]() | SRP SRP receptor / co-translational protein targeting / endoplasmic reticulum / signal peptide / RNA BINDING PROTEIN | |||||||||
機能・相同性 | ![]() SRP-dependent cotranslational protein targeting to membrane / signal recognition particle receptor complex / SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition / signal recognition particle, endoplasmic reticulum targeting / endoplasmic reticulum signal peptide binding / granulocyte differentiation / signal recognition particle binding / signal-recognition-particle GTPase / protein targeting to ER / 7S RNA binding ...SRP-dependent cotranslational protein targeting to membrane / signal recognition particle receptor complex / SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition / signal recognition particle, endoplasmic reticulum targeting / endoplasmic reticulum signal peptide binding / granulocyte differentiation / signal recognition particle binding / signal-recognition-particle GTPase / protein targeting to ER / 7S RNA binding / exocrine pancreas development / SRP-dependent cotranslational protein targeting to membrane / SRP-dependent cotranslational protein targeting to membrane, translocation / cytoplasmic microtubule / aminopeptidase activity / vesicle-mediated transport / neutrophil chemotaxis / intracellular protein transport / GDP binding / membrane => GO:0016020 / nuclear speck / serine-type endopeptidase activity / GTPase activity / endoplasmic reticulum membrane / nucleolus / GTP binding / Golgi apparatus / endoplasmic reticulum / ATP hydrolysis activity / nucleoplasm / nucleus / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.9 Å | |||||||||
![]() | Jomaa A / Ban N | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Molecular mechanism of cargo recognition and handover by the mammalian signal recognition particle. 著者: Ahmad Jomaa / Simon Eitzinger / Zikun Zhu / Sowmya Chandrasekar / Kan Kobayashi / Shu-Ou Shan / Nenad Ban / ![]() ![]() 要旨: Co-translational protein targeting to membranes by the signal recognition particle (SRP) is a universally conserved pathway from bacteria to humans. In mammals, SRP and its receptor (SR) have many ...Co-translational protein targeting to membranes by the signal recognition particle (SRP) is a universally conserved pathway from bacteria to humans. In mammals, SRP and its receptor (SR) have many additional RNA features and protein components compared to the bacterial system, which were recently shown to play regulatory roles. Due to its complexity, the mammalian SRP targeting process is mechanistically not well understood. In particular, it is not clear how SRP recognizes translating ribosomes with exposed signal sequences and how the GTPase activity of SRP and SR is regulated. Here, we present electron cryo-microscopy structures of SRP and SRP·SR in complex with the translating ribosome. The structures reveal the specific molecular interactions between SRP and the emerging signal sequence and the elements that regulate GTPase activity of SRP·SR. Our results suggest the molecular mechanism of how eukaryote-specific elements regulate the early and late stages of SRP-dependent protein targeting. #1: ![]() タイトル: Molecular mechanism of cargo recognition and handover by the mammalian signal recognition particle 著者: Jomaa A / Eitzinger S / Zhu Z / Chandrasekar S / Kobayashi K / Shan S / Ban N | |||||||||
履歴 |
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構造の表示
ムービー |
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構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
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ダウンロードとリンク
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マップデータ | ![]() | 320.9 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 24.6 KB 24.6 KB | 表示 表示 | ![]() |
画像 | ![]() | 87 KB | ||
Filedesc metadata | ![]() | 8.3 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | SRP-SR complex from 3D focused refinement on ribosome signal subtracted images | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.07 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
+全体 : SRP-SR complex
+超分子 #1: SRP-SR complex
+超分子 #2: SRP-SR complex
+超分子 #3: EM14S01-3B_G0054400.mRNA.1.CDS.1
+超分子 #4: Signal recognition particle receptor subunit
+分子 #1: SRP RNA
+分子 #2: Signal recognition particle 19
+分子 #3: EM14S01-3B_G0054400.mRNA.1.CDS.1
+分子 #4: Signal recognition particle subunit SRP68,Signal recognition part...
+分子 #5: Signal recognition particle receptor subunit beta
+分子 #6: Signal recognition particle 54 kDa protein
+分子 #7: SRP receptor subunit alpha
+分子 #8: Signal recognition particle subunit SRP72
+分子 #9: MAGNESIUM ION
+分子 #10: GUANOSINE-5'-TRIPHOSPHATE
+分子 #11: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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グリッド | モデル: Quantifoil R2/2 / 支持フィルム - 材質: CARBON / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 15 sec. |
凍結 | 凍結剤: ETHANE-PROPANE |
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電子顕微鏡法
顕微鏡 | TFS KRIOS |
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撮影 | フィルム・検出器のモデル: GATAN K3 (6k x 4k) / 平均電子線量: 50.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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画像解析
初期モデル | モデルのタイプ: OTHER / 詳細: From initial reconstruction |
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最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 3.9 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION / 使用した粒子像数: 155989 |
初期 角度割当 | タイプ: MAXIMUM LIKELIHOOD / ソフトウェア - 名称: RELION |
最終 角度割当 | タイプ: MAXIMUM LIKELIHOOD |