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基本情報
登録情報 | データベース: EMDB / ID: EMD-12702 | |||||||||
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タイトル | Murine supercomplex CIII2CIV in the assembled locked conformation | |||||||||
![]() | CIIICIV full, locked conformation | |||||||||
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機能・相同性 | ![]() subthalamus development / pons development / TP53 Regulates Metabolic Genes / Respiratory electron transport / cerebellar Purkinje cell layer development / pyramidal neuron development / response to mercury ion / mitochondrial respiratory chain complex III assembly / respiratory chain complex IV assembly / thalamus development ...subthalamus development / pons development / TP53 Regulates Metabolic Genes / Respiratory electron transport / cerebellar Purkinje cell layer development / pyramidal neuron development / response to mercury ion / mitochondrial respiratory chain complex III assembly / respiratory chain complex IV assembly / thalamus development / Cytoprotection by HMOX1 / mitochondrial respirasome assembly / response to cobalamin / respiratory chain complex IV / Mitochondrial protein degradation / : / regulation of oxidative phosphorylation / : / response to alkaloid / cytochrome-c oxidase / oxidative phosphorylation / quinol-cytochrome-c reductase / response to copper ion / response to glucagon / cellular respiration / ubiquinol-cytochrome-c reductase activity / mitochondrial electron transport, cytochrome c to oxygen / cytochrome-c oxidase activity / midbrain development / hypothalamus development / mitochondrial electron transport, ubiquinol to cytochrome c / response to hyperoxia / animal organ regeneration / electron transport coupled proton transport / enzyme regulator activity / ATP synthesis coupled electron transport / response to cadmium ion / response to electrical stimulus / lactation / respiratory electron transport chain / cerebellum development / response to hormone / response to activity / central nervous system development / hippocampus development / response to calcium ion / mitochondrial membrane / response to toxic substance / metalloendopeptidase activity / 2 iron, 2 sulfur cluster binding / myelin sheath / response to ethanol / response to oxidative stress / oxidoreductase activity / response to hypoxia / mitochondrial inner membrane / response to xenobiotic stimulus / copper ion binding / ubiquitin protein ligase binding / heme binding / protein-containing complex binding / protein-containing complex / mitochondrion / proteolysis / nucleoplasm / membrane / metal ion binding / cytosol 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.2 Å | |||||||||
![]() | Vercellino I / Sazanov LA | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: Structure and assembly of the mammalian mitochondrial supercomplex CIIICIV. 著者: Irene Vercellino / Leonid A Sazanov / ![]() 要旨: The enzymes of the mitochondrial electron transport chain are key players of cell metabolism. Despite being active when isolated, in vivo they associate into supercomplexes, whose precise role is ...The enzymes of the mitochondrial electron transport chain are key players of cell metabolism. Despite being active when isolated, in vivo they associate into supercomplexes, whose precise role is debated. Supercomplexes CIIICIV (refs. ), CICIII (ref. ) and CICIIICIV (respirasome) exist in mammals, but in contrast to CICIII and the respirasome, to date the only known eukaryotic structures of CIIICIV come from Saccharomyces cerevisiae and plants, which have different organization. Here we present the first, to our knowledge, structures of mammalian (mouse and ovine) CIIICIV and its assembly intermediates, in different conformations. We describe the assembly of CIIICIV from the CIII precursor to the final CIIICIV conformation, driven by the insertion of the N terminus of the assembly factor SCAF1 (ref. ) deep into CIII, while its C terminus is integrated into CIV. Our structures (which include CICIII and the respirasome) also confirm that SCAF1 is exclusively required for the assembly of CIIICIV and has no role in the assembly of the respirasome. We show that CIII is asymmetric due to the presence of only one copy of subunit 9, which straddles both monomers and prevents the attachment of a second copy of SCAF1 to CIII, explaining the presence of one copy of CIV in CIIICIV in mammals. Finally, we show that CIII and CIV gain catalytic advantage when assembled into the supercomplex and propose a role for CIIICIV in fine tuning the efficiency of electron transfer in the electron transport chain. | |||||||||
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構造の表示
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構造ビューア | EMマップ: ![]() ![]() ![]() |
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マップデータ | ![]() | 4 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 43.6 KB 43.6 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 18.6 KB | 表示 | ![]() |
画像 | ![]() | 171.3 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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「今月の分子」の関連する項目 |
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注釈 | CIIICIV full, locked conformation | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.064 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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試料の構成要素
+全体 : Murine full supercomplex CIII2CIV in the locked conformation
+超分子 #1: Murine full supercomplex CIII2CIV in the locked conformation
+分子 #1: Cytochrome b-c1 complex subunit 1, mitochondrial
+分子 #2: Cytochrome b-c1 complex subunit 2, mitochondrial
+分子 #3: Cytochrome b
+分子 #4: Cytochrome c1, heme protein, mitochondrial
+分子 #5: Cytochrome b-c1 complex subunit Rieske, mitochondrial
+分子 #6: Cytochrome b-c1 complex subunit 7
+分子 #7: Cytochrome b-c1 complex subunit 8
+分子 #8: Cytochrome b-c1 complex subunit 6, mitochondrial
+分子 #9: Cytochrome b-c1 complex subunit 9
+分子 #10: Cytochrome b-c1 complex subunit 10
+分子 #11: Cytochrome b-c1 complex subunit 9
+分子 #12: Cox7a2l protein
+分子 #13: Cytochrome c oxidase subunit 1
+分子 #14: Cytochrome c oxidase subunit 2
+分子 #15: Cytochrome c oxidase subunit 3
+分子 #16: Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
+分子 #17: Cytochrome c oxidase subunit 5A, mitochondrial
+分子 #18: Cytochrome c oxidase subunit 5B, mitochondrial
+分子 #19: Cytochrome c oxidase subunit 6A2, mitochondrial
+分子 #20: Cytochrome c oxidase subunit 6B1
+分子 #21: Cytochrome c oxidase subunit 6C
+分子 #22: Cytochrome c oxidase subunit 7B, mitochondrial
+分子 #23: Cytochrome c oxidase subunit 7C, mitochondrial
+分子 #24: Cytochrome c oxidase subunit 8B, mitochondrial
+分子 #25: 1,2-Distearoyl-sn-glycerophosphoethanolamine
+分子 #26: CARDIOLIPIN
+分子 #27: PROTOPORPHYRIN IX CONTAINING FE
+分子 #28: HEME C
+分子 #29: FE2/S2 (INORGANIC) CLUSTER
+分子 #30: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
+分子 #31: TRISTEAROYLGLYCEROL
+分子 #32: COPPER (II) ION
+分子 #33: SODIUM ION
+分子 #34: HEME-A
+分子 #35: MAGNESIUM ION
+分子 #36: DINUCLEAR COPPER ION
+分子 #37: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
濃度 | 4 mg/mL | |||||||||||||||
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緩衝液 | pH: 7.7 構成要素:
詳細: CHAPS was added only upon freezing | |||||||||||||||
グリッド | モデル: Quantifoil R0.6/1 / 材質: COPPER / メッシュ: 300 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: CONTINUOUS / 支持フィルム - Film thickness: 1.0 nm / 前処理 - タイプ: GLOW DISCHARGE | |||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 検出モード: INTEGRATING / 撮影したグリッド数: 1 / 実像数: 7245 / 平均露光時間: 1.17 sec. / 平均電子線量: 90.66 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | C2レンズ絞り径: 50.0 µm / 最大 デフォーカス(補正後): 2.7 µm / 最小 デフォーカス(補正後): 0.2 µm / 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 1.6 µm / 最小 デフォーカス(公称値): 0.4 µm / 倍率(公称値): 75000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |