登録情報 データベース : EMDB / ID : EMD-11824 構造の表示 ダウンロードとリンクタイトル Atomic structure of the poxvirus transcription pre-initiation complex in the initially melted state マップデータComposite map from Phenix.combine_focused_maps 詳細 試料複合体 : Vaccinia transcription pre-initiation complexタンパク質・ペプチド : x 11種DNA : x 2種リガンド : x 2種 詳細機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
virion component => GO:0044423 / hydrolase activity, acting on glycosyl bonds / viral transcription / RNA polymerase I activity / metabolic process / DNA-directed RNA polymerase complex / RNA polymerase II activity / nucleotidyltransferase activity / virion component / DNA-templated transcription termination ... virion component => GO:0044423 / hydrolase activity, acting on glycosyl bonds / viral transcription / RNA polymerase I activity / metabolic process / DNA-directed RNA polymerase complex / RNA polymerase II activity / nucleotidyltransferase activity / virion component / DNA-templated transcription termination / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / membrane => GO:0016020 / DNA-binding transcription factor activity / DNA-templated transcription / positive regulation of DNA-templated transcription / DNA binding / zinc ion binding / ATP binding / metal ion binding 類似検索 - 分子機能 Poxvirus early transcription factor, large subunit / Poxvirus early transcription factor (VETF), large subunit / RNA polymerase-associated transcription specificity factor Rap94 / RNA polymerase-associated transcription specificity factor, Rap94 / DNA-directed RNA polymerase, 18kDa subunit, poxviral / DNA-directed RNA polymerase, 35kDa subunit, poxviral / RNA polymerase, 22kDa subunit, poxviral / DNA-directed RNA polymerase, 19kDa subunit, poxviral / DNA-directed RNA polymerase, 7kDa polypeptide, chordopoxviral / RNA polymerase, 30kDa subunit, chordopox-type ... Poxvirus early transcription factor, large subunit / Poxvirus early transcription factor (VETF), large subunit / RNA polymerase-associated transcription specificity factor Rap94 / RNA polymerase-associated transcription specificity factor, Rap94 / DNA-directed RNA polymerase, 18kDa subunit, poxviral / DNA-directed RNA polymerase, 35kDa subunit, poxviral / RNA polymerase, 22kDa subunit, poxviral / DNA-directed RNA polymerase, 19kDa subunit, poxviral / DNA-directed RNA polymerase, 7kDa polypeptide, chordopoxviral / RNA polymerase, 30kDa subunit, chordopox-type / RNA polymerase, 132kDa subunit, poxvirus-type / RNA polymerase, 30kDa subunit, chordopox-type, N-terminal / Poxvirus DNA-directed RNA polymerase, 18 kD subunit / Poxvirus DNA-directed RNA polymerase, 35 kD subunit / Poxvirus RNA polymerase 22 kDa subunit / Poxvirus DNA-directed RNA polymerase 19 kDa subunit / Chordopoxvirus DNA-directed RNA polymerase 7 kDa polypeptide (RPO7) / Poxvirus DNA dependent RNA polymerase 30kDa subunit / Poxvirus DNA dependent RNA polymerase / Helicase/UvrB, N-terminal / Type III restriction enzyme, res subunit / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / DEAH-box subfamily ATP-dependent helicases signature. / Zinc finger TFIIS-type signature. / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 5 / Zinc finger, TFIIS-type / Transcription factor S-II (TFIIS) / Zinc finger TFIIS-type profile. / C2C2 Zinc finger / RNA polymerase Rpb1, clamp domain superfamily / RPB6/omega subunit-like superfamily / Helicase conserved C-terminal domain / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 4 / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 5 / RNA polymerase, beta subunit, conserved site / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerase Rpb2, OB-fold / RNA polymerase Rpb2, domain 7 / RNA polymerase Rpb2, domain 3 / RNA polymerases beta chain signature. / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain / DNA-directed RNA polymerase, subunit 2 / DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily / RNA polymerase Rpb2, domain 6 / helicase superfamily c-terminal domain / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性 DNA-directed RNA polymerase subunit / DNA-directed RNA polymerase 30 kDa polypeptide / RNA polymerase-associated transcription-specificity factor RAP94 / DNA-directed RNA polymerase 147 kDa polypeptide / DNA-directed RNA polymerase 35 kDa subunit / DNA-directed RNA polymerase / DNA-directed RNA polymerase 18 kDa subunit / ETF large subunit / DNA-directed RNA polymerase 19 kDa subunit / ATP-dependent helicase VETFS / DNA-directed RNA polymerase 7 kDa subunit 類似検索 - 構成要素生物種 Vaccinia virus GLV-1h68 (ウイルス)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 2.8 Å 詳細 データ登録者Grimm C / Bartuli J / Fischer U 資金援助 ドイツ, 1件 詳細 詳細を隠すOrganization Grant number 国 German Research Foundation (DFG) Fi-573/20-1 ドイツ
引用ジャーナル : Nat Struct Mol Biol / 年 : 2021タイトル : Structural basis of the complete poxvirus transcription initiation process.著者 : Clemens Grimm / Julia Bartuli / Bettina Boettcher / Aladar A Szalay / Utz Fischer / 要旨 : Poxviruses express their genes in the cytoplasm of infected cells using a virus-encoded multi-subunit polymerase (vRNAP) and unique transcription factors. We present cryo-EM structures that uncover ... Poxviruses express their genes in the cytoplasm of infected cells using a virus-encoded multi-subunit polymerase (vRNAP) and unique transcription factors. We present cryo-EM structures that uncover the complete transcription initiation phase of the poxvirus vaccinia. In the pre-initiation complex, the heterodimeric early transcription factor VETFs/l adopts an arc-like shape spanning the polymerase cleft and anchoring upstream and downstream promoter elements. VETFI emerges as a TBP-like protein that inserts asymmetrically into the DNA major groove, triggers DNA melting, ensures promoter recognition and enforces transcription directionality. The helicase VETFs fosters promoter melting and the phospho-peptide domain (PPD) of vRNAP subunit Rpo30 enables transcription initiation. An unprecedented upstream promoter scrunching mechanism assisted by the helicase NPH-I probably fosters promoter escape and transition into elongation. Our structures shed light on unique mechanisms of poxviral gene expression and aid the understanding of thus far unexplained universal principles in transcription. 履歴 登録 2020年10月9日 - ヘッダ(付随情報) 公開 2021年10月6日 - マップ公開 2021年10月6日 - 更新 2021年10月20日 - 現状 2021年10月20日 処理サイト : PDBe / 状態 : 公開
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