Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Reversible lipid-mediated pH-gating of connexin-46/50 by cryo-EM.
Journal, issue, pages	Nat Commun, Year 2026
Publish date	Jan 12, 2026
Authors	Joshua M Jarodsky / Janette B Myers / Steve L Reichow /
PubMed Abstract	Gap junctions, formed by connexin proteins, establish direct electrical and metabolic coupling between cells, enabling coordinated tissue responses. These channels universally respond to ...Gap junctions, formed by connexin proteins, establish direct electrical and metabolic coupling between cells, enabling coordinated tissue responses. These channels universally respond to intracellular pH changes, closing under acidic conditions to limit the spread of cytotoxic signals during cellular stress, such as ischemia. Using cryo-electron microscopy (cryo-EM), we uncover insights into the structural mechanism of pH-gating in native lens connexin-46/50 (Cx46/50) gap junctions. Mild acidification drives lipid infiltration into the channel pore, displacing the N-terminal (NT) domain and stabilizing pore closure. Lipid involvement is shown to be both essential and fully reversible. Structural transitions involve an ensemble of gated states formed through non-cooperative NT domain movement as well as minor populations of a distinct destabilized open-state. These findings provide molecular insights into pH-gating dynamics, illustrating how structural changes may regulate gap junction function under cellular stress and linking Cx46/50 dysregulation to age-related cataract formation.
External links	Nat Commun / PubMed:41526355
Methods	EM (single particle)
Resolution	2.0 - 2.7 Å
Structure data	73876 9z7p 9z7w EMDB-73876: Stable open sheep connexin-46/50 in amphipol at low pH PDB-9z7p: Stable open sheep connexin-46 in amphipol at low pH PDB-9z7w: Stable open sheep connexin-50 in amphipol at low pH Method: EM (single particle) / Resolution: 2.7 Å 73885 9z81 9z82 EMDB-73885: Stable open state sheep connexin-46/50 in DMPC nanodiscs at neutral pH PDB-9z81: Stable open state sheep connexin-46 in DMPC nanodiscs at neutral pH PDB-9z82: Stable open state sheep connexin-50 in DMPC nanodiscs at neutral pH Method: EM (single particle) / Resolution: 2.0 Å 73896 9z8f 9z8l EMDB-73896, PDB-9z8f: Destabilized open state sheep connexin-46 in DMPC nanodiscs at neutral pH PDB-9z8l: Destabilized open state sheep connexin-50 in DMPC nanodiscs at neutral pH Method: EM (single particle) / Resolution: 2.2 Å 73900 9z8m 9z9b EMDB-73900: Gated state sheep connexin-46/50 in DMPC nanodiscs at neutral pH PDB-9z8m: Gated state sheep connexin-46 in DMPC nanodiscs at neutral pH PDB-9z9b: Gated state sheep connexin-50 in DMPC nanodiscs at neutral pH Method: EM (single particle) / Resolution: 2.7 Å 73942 9z9g 9z9h EMDB-73942: Stable open state sheep connexin-46/50 in DMPC nanodiscs at low pH PDB-9z9g: Stable open state sheep connexin-46 in DMPC nanodiscs at low pH PDB-9z9h: Stable open state sheep connexin-50 in DMPC nanodiscs at low pH Method: EM (single particle) / Resolution: 2.5 Å 73957 9z9s 9z9w EMDB-73957: Destabilized open state sheep connexin-46/50 in DMPC nanodiscs at low pH PDB-9z9s: Destabilized open state sheep connexin-46 in DMPC nanodiscs at low pH PDB-9z9w: Destabilized open state sheep connexin-50 in DMPC nanodiscs at low pH Method: EM (single particle) / Resolution: 2.2 Å 73962 9z9x EMDB-73962: Gated state sheep connexin-46/50 in DMPC nanodiscs at low pH PDB-9z9x: Gated state sheep connexin-46 in DMPC nanodiscs at low pH Method: EM (single particle) / Resolution: 2.3 Å 73965 9za3 9za4 EMDB-73965: Asymmetrically gated state sheep connexin-46/50 in DMPC nanodiscs at low pH PDB-9za3: Asymmetrically gated state sheep connexin-46 in DMPC nanodiscs at low pH PDB-9za4: Asymmetrically gated state sheep connexin-50 in DMPC nanodiscs at low pH Method: EM (single particle) / Resolution: 2.4 Å PDB-9z9y: Gated state sheep connexin-50 in DMPC nanodiscs at low pH Method: ELECTRON MICROSCOPY / Resolution: 2.3 Å
Chemicals	ChemComp-MC3: 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE / phospholipidYM ChemComp-HOH*: WATER
Source	ovis aries (sheep)
Keywords	MEMBRANE PROTEIN / connexin / gap junction / cryo-EM / pH regulation / lipid gating / large-pore channel