EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Reversible lipid-mediated pH-gating of connexin-46/50 by cryo-EM.
ジャーナル・号・ページ	Nat Commun, Year 2026
掲載日	2026年1月12日
著者	Joshua M Jarodsky / Janette B Myers / Steve L Reichow /
PubMed 要旨	Gap junctions, formed by connexin proteins, establish direct electrical and metabolic coupling between cells, enabling coordinated tissue responses. These channels universally respond to ...Gap junctions, formed by connexin proteins, establish direct electrical and metabolic coupling between cells, enabling coordinated tissue responses. These channels universally respond to intracellular pH changes, closing under acidic conditions to limit the spread of cytotoxic signals during cellular stress, such as ischemia. Using cryo-electron microscopy (cryo-EM), we uncover insights into the structural mechanism of pH-gating in native lens connexin-46/50 (Cx46/50) gap junctions. Mild acidification drives lipid infiltration into the channel pore, displacing the N-terminal (NT) domain and stabilizing pore closure. Lipid involvement is shown to be both essential and fully reversible. Structural transitions involve an ensemble of gated states formed through non-cooperative NT domain movement as well as minor populations of a distinct destabilized open-state. These findings provide molecular insights into pH-gating dynamics, illustrating how structural changes may regulate gap junction function under cellular stress and linking Cx46/50 dysregulation to age-related cataract formation.
リンク	Nat Commun / PubMed:41526355
手法	EM (単粒子)
解像度	2.0 - 2.7 Å
構造データ	73876 9z7p 9z7w EMDB-73876: Stable open sheep connexin-46/50 in amphipol at low pH PDB-9z7p: Stable open sheep connexin-46 in amphipol at low pH PDB-9z7w: Stable open sheep connexin-50 in amphipol at low pH 手法: EM (単粒子) / 解像度: 2.7 Å 73885 9z81 9z82 EMDB-73885: Stable open state sheep connexin-46/50 in DMPC nanodiscs at neutral pH PDB-9z81: Stable open state sheep connexin-46 in DMPC nanodiscs at neutral pH PDB-9z82: Stable open state sheep connexin-50 in DMPC nanodiscs at neutral pH 手法: EM (単粒子) / 解像度: 2.0 Å 73896 9z8f 9z8l EMDB-73896, PDB-9z8f: Destabilized open state sheep connexin-46 in DMPC nanodiscs at neutral pH PDB-9z8l: Destabilized open state sheep connexin-50 in DMPC nanodiscs at neutral pH 手法: EM (単粒子) / 解像度: 2.2 Å 73900 9z8m 9z9b EMDB-73900: Gated state sheep connexin-46/50 in DMPC nanodiscs at neutral pH PDB-9z8m: Gated state sheep connexin-46 in DMPC nanodiscs at neutral pH PDB-9z9b: Gated state sheep connexin-50 in DMPC nanodiscs at neutral pH 手法: EM (単粒子) / 解像度: 2.7 Å 73942 9z9g 9z9h EMDB-73942: Stable open state sheep connexin-46/50 in DMPC nanodiscs at low pH PDB-9z9g: Stable open state sheep connexin-46 in DMPC nanodiscs at low pH PDB-9z9h: Stable open state sheep connexin-50 in DMPC nanodiscs at low pH 手法: EM (単粒子) / 解像度: 2.5 Å 73957 9z9s 9z9w EMDB-73957: Destabilized open state sheep connexin-46/50 in DMPC nanodiscs at low pH PDB-9z9s: Destabilized open state sheep connexin-46 in DMPC nanodiscs at low pH PDB-9z9w: Destabilized open state sheep connexin-50 in DMPC nanodiscs at low pH 手法: EM (単粒子) / 解像度: 2.2 Å 73962 9z9x EMDB-73962: Gated state sheep connexin-46/50 in DMPC nanodiscs at low pH PDB-9z9x: Gated state sheep connexin-46 in DMPC nanodiscs at low pH 手法: EM (単粒子) / 解像度: 2.3 Å 73965 9za3 9za4 EMDB-73965: Asymmetrically gated state sheep connexin-46/50 in DMPC nanodiscs at low pH PDB-9za3: Asymmetrically gated state sheep connexin-46 in DMPC nanodiscs at low pH PDB-9za4: Asymmetrically gated state sheep connexin-50 in DMPC nanodiscs at low pH 手法: EM (単粒子) / 解像度: 2.4 Å PDB-9z9y: Gated state sheep connexin-50 in DMPC nanodiscs at low pH 手法: ELECTRON MICROSCOPY / 解像度: 2.3 Å
化合物	ChemComp-MC3: 1,2-DIMYRISTOYL-RAC-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-HOH*: WATER
由来	ovis aries (ヒツジ)
キーワード	MEMBRANE PROTEIN / connexin / gap junction / cryo-EM / pH regulation / lipid gating / large-pore channel