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-Structure paper
| タイトル | Asymmetric isopeptide bond steers directional genomic RNA egress from icosahedral virus. |
|---|---|
| ジャーナル・号・ページ | Sci Adv, Vol. 11, Issue 50, Page eady4104, Year 2025 |
| 掲載日 | 2025年12月12日 |
著者 | Sean M Braet / Varun Venkatakrishnan / Ranita Ramesh / Molly A Clawson / Tatiana N Laremore / Sek-Man Wong / Ganesh S Anand / ![]() |
| PubMed 要旨 | Icosahedral RNA viruses rely upon essential asymmetries for directional genome egress into the host cell. How these asymmetric egress points are built into the quaternary assembly of the virion is ...Icosahedral RNA viruses rely upon essential asymmetries for directional genome egress into the host cell. How these asymmetric egress points are built into the quaternary assembly of the virion is unknown. Here, we capture the structure and dynamics of a partially expanded virus disassembly intermediate, poised to release its spring-loaded genomic RNA. The structure shows highly localized density of RNA underneath surface fivefold axes on one-half of the viral particle. This polarity in RNA distribution is associated with a unique interchain isopeptide bond (glutamic acid-lysine), which flanks conserved high-affinity RNA binding sites. This singular isopeptide bond at the asymmetric egress site confers an essential "loaded-die" feature that is critical for steering genomic RNA egress into the host cell for virus propagation. |
リンク | Sci Adv / PubMed:41385643 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.87 - 3.62 Å |
| 構造データ | EMDB-72428, PDB-9y2z: EMDB-72430, PDB-9y31: |
| 由来 |
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キーワード | VIRUS / Icosahedral non-enveloped ssRNA virus / Expansion intermediate / VIRAL PROTEIN |
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turnip crinkle virus (ウイルス)
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