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TitleStructural basis for the assembly and translocation of the Vip1-Vip2 insecticidal toxin from Bacillus thuringiensis.
Journal, issue, pagesNat Commun, Year 2026
Publish dateMar 30, 2026
AuthorsTing Zhao / Zeyu Wang / Jing Ren / Xianle Han / Linpeng Li / Zheng Liu / Jie Zhang / Peng Chen /
PubMed AbstractInsecticidal toxins from Bacillus thuringiensis (Bt) have been extensively and successfully used in genetically engineered crops for decades but continue to face challenges from the adaptive ...Insecticidal toxins from Bacillus thuringiensis (Bt) have been extensively and successfully used in genetically engineered crops for decades but continue to face challenges from the adaptive resistance in the insect population. The Bt binary toxin Vip1Ad1(Vip1) and Vip2Ag1(Vip2), a promising next-generation candidate gene combination for transgenic crops, have demonstrated high efficacy against the destructive coleopteran pest white grubs, however, their mode of action remains largely elusive. In this study, we report cryo-EM structures of the heptameric Vip1-pore and Vip2-bound Vip1-pore complex, capturing a series of putative assembly-related intermediates that suggest a binary toxin assembly and translocation pathway. Together with structure-guided mutagenesis, these data provide insights into a sequential assembly of binary complex and a sequence-independent translocation mechanism. Proof-of-principle experiments showed successful delivery of a desired protein cargo into host cells based on the mini-Vip2-Vip1 pore system, paving the way for developing much needed extracellular pesticidal protein delivery platforms. These findings not only clarify the assembly and translocation mechanism of the binary insecticidal toxin pair but also offer an excellent alternative model to investigate human-pathogenic pore-forming toxins because of its similarity and biosafety.
External linksNat Commun / PubMed:41912579
MethodsEM (single particle)
Resolution2.4 - 3.31 Å
Structure data

65007

9veg

EMDB-65007, PDB-9veg:
Structural basis for the assembly and translocation of the Vip1-Vip2 insecticidal binary toxin from Bacillus thuringiensis
Method: EM (single particle) / Resolution: 3.05 Å

65009

9vej

EMDB-65009, PDB-9vej:
Structural basis for the assembly and translocation of the Vip1-Vip2 insecticidal binary toxin from Bacillus thuringiensis
Method: EM (single particle) / Resolution: 2.4 Å

65010

9vek

EMDB-65010, PDB-9vek:
Structural basis for the assembly and translocation of the Vip1-Vip2 insecticidal binary toxin from Bacillus thuringiensis
Method: EM (single particle) / Resolution: 3.31 Å

Chemicals

ChemComp-CA:
Unknown entry

Source
  • bacillus thuringiensis (bacteria)
KeywordsTOXIN / binary toxin / biopesticide

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