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TitleStructural N- and O-glycans revealed by high-resolution cryo-EM analysis of tubular mastigonemes.
Journal, issue, pagesScience, Page eaef4958, Year 2026
Publish dateApr 23, 2026
AuthorsJunhao Huang / Hui Tao / Sheng Chen / Yahua Cui / Yiran Xu / Chuangye Yan / Nieng Yan /
PubMed AbstractThe chemical complexity and non-templated biosynthesis of glycans have posed significant challenges for establishing sequence-structure relationships. Here we report cryo-EM structures of tubular ...The chemical complexity and non-templated biosynthesis of glycans have posed significant challenges for establishing sequence-structure relationships. Here we report cryo-EM structures of tubular mastigonemes from a golden alga species, , in which a large number of N- and O-glycans are resolved at 1.8-2.2 Å resolution. Beyond high-mannose and complex N-glycans, we identify a non-canonical N-glycan on the Ala--Asp (AD) motif. The surface spikes comprise dense O-glycans coating PSXX tetrapeptide repeats, with two glycans linked on trihydroxylated proline and one on serine per repeat. In addition to various types of sugars and their covalent modifiers, water molecules (>10% of resolved volume) and cations are clearly resolved and mediate the structural assembly. Our study establishes a framework for investigating glycan folding in high-order biological assemblies.
External linksScience / PubMed:42024774
MethodsEM (single particle)
Resolution2.17 Å
Structure data

64106

9ufe

EMDB-64106, PDB-9ufe:
Cryo-EM structure of the tubular mastigoneme (the central tube) from golden algae 2.17 angstrom resolution
Method: EM (single particle) / Resolution: 2.17 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-2PO:
PHOSPHONATE

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • ochromonas danica (eukaryote)
KeywordsCARBOHYDRATE / Cryo-EM / Tubular Mastigoneme / Golden algae / N-Glycans

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