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TitleWhen VPS34 complexes double down: Two RAB5s for VPS34-CII, two RAB1s for NRBF2-dimerized VPS34-CI
Journal, issue, pagesTo Be Published
Publish dateJul 9, 2025 (structure data deposition date)
AuthorsSpokaite S / Ohashi Y / Dessus AN / Bourguet M / Williams RL
External linksSearch PubMed
MethodsEM (single particle)
Resolution3.33 - 3.8 Å
Structure data

EMDB-54307: VPS34-CI bound to NRBF2 and RAB1A, focused refinement on the kinase arm
Method: EM (single particle) / Resolution: 3.55 Å

EMDB-54308: VPS34-CI bound to NRBF2 and RAB1A, focused refinement on the adaptor arm
Method: EM (single particle) / Resolution: 3.68 Å

EMDB-54309: VPS34-CI bound to NRBF2 and RAB1A, focused refinement on the interface between RAB1A and VPS34
Method: EM (single particle) / Resolution: 3.49 Å

EMDB-54310: VPS34-CI bound to NRBF2 and RAB1A, consensus refinement
Method: EM (single particle) / Resolution: 3.67 Å

EMDB-54311: VPS34-CI bound to NRBF2 and RAB1A; composite unsharpened map
Method: EM (single particle) / Resolution: 3.55 Å

EMDB-54323: VPS34-CI bound to NRBF2 MIT domain (residues 1-79), focused refinement on the VPS34 kinase domain and VPS15 pseudokinase domain
Method: EM (single particle) / Resolution: 3.54 Å

EMDB-54324: VPS34-CI bound to NRBF2 MIT domain (residues 1-79), focused refinement on the VPS15 WD40 domain, BECLIN1 BARA domain, and ATG14L CTD
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-54325: VPS34-CI bound to NRBF2 MIT domain (residues 1-79, focused refinement on the VPS15 helical solenoid, and ATG14L/BECLIN1 CC1 and N-terminal domains
Method: EM (single particle) / Resolution: 3.33 Å

EMDB-54326: VPS34-CI bound to NRBF2 MIT domain (residues 1-79), consensus refinement
Method: EM (single particle) / Resolution: 3.78 Å

EMDB-54357: VPS34-CI bound to NRBF2 and RAB1A, primary composite map processed with CryoTEN
PDB-9rx4: VPS34-CI bound to NRBF2 and RAB1A
Method: EM (single particle) / Resolution: 3.67 Å

EMDB-54360: VPS34-CI bound to NRBF2 MIT domain (residues 1-79), unsharpened composite EM map
PDB-9rx7: VPS34-CI bound to NRBF2 MIT domain (residues 1-79)
Method: EM (single particle) / Resolution: 3.33 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-MYR:
MYRISTIC ACID

ChemComp-ZN:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Source
  • homo sapiens (human)
KeywordsSIGNALING PROTEIN / Lipid kinase / GTPase / kinase / autophagy

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