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TitleThe single-particle cryo-EM structures of a bacterial cyanide dihydratase and a fungal cyanide hydratase.
Journal, issue, pagesStructure, Year 2026
Publish dateFeb 17, 2026
AuthorsSantiago Justo Arevalo / Valeria Valle-Riestra Felice / Mikaela Barahona Acuña / Katia Ordinola Flores / Mauro Quiñones Aguilar / Andrea Balan / Chuck Shaker Farah /
PubMed AbstractCyanide is widely used in industries due to its affinity for metals, a property that also underlies its toxicity. Industries, therefore, must reduce cyanide concentration before the final disposal of ...Cyanide is widely used in industries due to its affinity for metals, a property that also underlies its toxicity. Industries, therefore, must reduce cyanide concentration before the final disposal of wastewater. Physical, chemical, and biological methods have been developed for this; however, knowledge about the structure of enzymes involved in cyanide degradation remains limited. Structural characterization of these proteins could facilitate the development of enzymes with enhanced bioremediation potential. Here, we present the single-particle cryo-electron microscopy structures of a cyanide dihydratase from Bacillus safensis and a cyanide hydratase from Gloeocercospora sorghi at 2.2 Å and 2.0 Å resolution, respectively. We provide a comprehensive description and comparative analysis alongside all previously determined nitrilase structures. Importantly, our full-length structures reveal new features in the C-terminal as well as specific intermolecular interactions between protomer interfaces and within the helix lumen. Finally, our findings offer insights into the reaction mechanisms of these two enzymes.
External linksStructure / PubMed:41709456
MethodsEM (single particle)
Resolution2.04 Å
Structure data

70376

9odt

EMDB-70376, PDB-9odt:
The structure of a Bacterial Cyanide Dihydratase from Bacillus safensis PER-URP-08
Method: EM (single particle) / Resolution: 2.04 Å

70421

9ofa

EMDB-70421, PDB-9ofa:
The structure of a Fungal Cyanide Hydratase from Gloeocercospora sorghi
Method: EM (single particle) / Resolution: 2.04 Å

Source
  • bacillus safensis (bacteria)
  • microdochium sorghi (fungus)
KeywordsHYDROLASE / CynD / nitrilase / biorremediation / oligomer / CynH

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